FvH4_6g05250

Protein of unknown function (DUF1068)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016358
Physical Location & Seq
Forward (+)
2962166 .. 2963841
1676 bp
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UTR
Exon/CDS
Intron
FvH4_6g05250.t1

Sequence Viewer

Length: 519 bp
ATGGCTCATCCTCAAGACCATGGCTTTTGTTCTAATGGTGCTGCGAGGCTGGCTTTGGTTTTGGTTGGTGTGTGTTTGGTTGGTTACACCGTACGTCAATCACTACATTGGCGCTTTATTATGGAGACTAGTTCATGTCCTCCATGTCTTTGTGACTGTTCAGATGAATCTGCCCTCGTACCTATAGATATCATCAACAGCTCATTTACAGACTGTGGAAAACATGAACCAGAAATGAAAGAAGAAATGGAGAAGGATATCGTCGCATTGATGTCGGAAGAGCTAAGTTTGCAGGAGGCTGTTGCAAATGAAACCCTGCAACGGAGCGAAGAGTTGTTGAGAACTGCTAGGAGTGTCTCTTTACACTACCAAAAAGAAGCTGAAAAATGTACTGCAGGAGTGGAAACTTGTGAAGAAGCAAGGGAGAGGGCTCAAGGGCAACTTGCAGAAGAGCTCAAGGCTTCAGAAATATGGGAGAGACGAGCTCGAGAGCTCGGTTGGGAAGAAATGACAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1068

Protein Analysis

173

Amino Acids

19.33

Weight (kDa)

4.72

Isoelectric Point (pI)

61.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1068 PF06364 14 - 169 2.2e-55 Protein of unknown function (DUF1068)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016358)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05250
malus_domestica MD04G1210200.v1.1
prunus_persica Prupe.6G331100_v2.0.a1 Prupe.6G331100_v2.0.a1
pyrus_communis pycom04g18630
rosa_chinensis RchiOBHm_Chr3g0453981
rosa_multiflora Rmu_sc0003556.1_g000034
rosa_roxburghii Rroxscaffold_6G00430800
rosa_rugosa Rorug02G0652100 Rorug02G0652200
rosa_samantha Rh3AG054700 Rh3BG056300 Rh3CG055500 Rh3DG056800
rosa_wichuraiana Rw3G004200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 447
AfaI GTAC 3 cut(s) 93, 180, 391
AfiI CCNNNNNNNGG 1 cut(s) 321
AhlI ACTAGT 1 cut(s) 128
AluBI AGCT 6 cut(s) 201, 283, 380, 454, 485, 493
AluI AGCT 6 cut(s) 201, 283, 380, 454, 485, 493
Alw21I GWGCWC 3 cut(s) 456, 487, 495
Alw26I GTCTC 3 cut(s) 119, 361, 472
Ama87I CYCGRG 1 cut(s) 486
ApeKI GCWGC 1 cut(s) 41
AspLEI GCGC 1 cut(s) 114
AvaI CYCGRG 1 cut(s) 486
BanII GRGCYC 4 cut(s) 433, 456, 487, 495
Bbv12I GWGCWC 3 cut(s) 456, 487, 495
BbvI GCAGC 1 cut(s) 28
BcgI CGANNNNNNTGC 2 cut(s) 255, 289
BcoDI GTCTC 3 cut(s) 119, 361, 472
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 2 cut(s) 129, 348
BfmI CTRYAG 2 cut(s) 183, 393
BfoI RGCGCY 1 cut(s) 115
BisI GCNGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 43
BmeT110I CYCGRG 1 cut(s) 486
BplI GAGNNNNNCTC 2 cut(s) 469, 501
BpuEI CTTGAG 2 cut(s) 417, 440
BsaJI CCNNGG 1 cut(s) 19
Bsc4I CCNNNNNNNGG 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 19
BseGI GGATG 1 cut(s) 7
BseLI CCNNNNNNNGG 1 cut(s) 321
BseXI GCAGC 1 cut(s) 28
BsiHKAI GWGCWC 3 cut(s) 456, 487, 495
BsiHKCI CYCGRG 1 cut(s) 486
BsiWI CGTACG 1 cut(s) 91
BslI CCNNNNNNNGG 1 cut(s) 321
BsmAI GTCTC 3 cut(s) 119, 361, 472
BsmBI CGTCTC 1 cut(s) 472
BsoBI CYCGRG 1 cut(s) 486
Bsp1286I GDGCHC 4 cut(s) 433, 456, 487, 495
Bsp19I CCATGG 1 cut(s) 19
BspMAI CTGCAG 1 cut(s) 397
BspQI GCTCTTC 2 cut(s) 273, 444
BssECI CCNNGG 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 19
Bst4CI ACNGT 3 cut(s) 91, 158, 215
Bst6I CTCTTC 3 cut(s) 273, 324, 444
BstC8I GCNNGC 1 cut(s) 51
BstDEI CTNAG 1 cut(s) 284
BstDSI CCRYGG 1 cut(s) 19
BstF5I GGATG 1 cut(s) 7
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 1 cut(s) 114
BstMAI GTCTC 3 cut(s) 119, 361, 472
BstMWI GCNNNNNNNGC 2 cut(s) 50, 289
BstSFI CTRYAG 2 cut(s) 183, 393
BstV1I GCAGC 1 cut(s) 28
BtgI CCRYGG 1 cut(s) 19
BtsCI GGATG 1 cut(s) 7
Cac8I GCNNGC 1 cut(s) 51
CfoI GCGC 1 cut(s) 114
Csp6I GTAC 3 cut(s) 92, 179, 390
CviAII CATG 4 cut(s) 20, 135, 144, 224
CviQI GTAC 3 cut(s) 92, 179, 390
DdeI CTNAG 1 cut(s) 284
Eam1104I CTCTTC 3 cut(s) 273, 324, 444
EarI CTCTTC 3 cut(s) 273, 324, 444
Ecl136II GAGCTC 3 cut(s) 454, 485, 493
Eco130I CCWWGG 1 cut(s) 19
Eco24I GRGCYC 4 cut(s) 433, 456, 487, 495
Eco32I GATATC 2 cut(s) 190, 259
Eco53kI GAGCTC 3 cut(s) 454, 485, 493
Eco57I CTGAAG 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 486
EcoICRI GAGCTC 3 cut(s) 454, 485, 493
EcoRV GATATC 2 cut(s) 190, 259
EcoT14I CCWWGG 1 cut(s) 19
EcoT38I GRGCYC 4 cut(s) 433, 456, 487, 495
ErhI CCWWGG 1 cut(s) 19
Esp3I CGTCTC 1 cut(s) 472
FaeI CATG 4 cut(s) 23, 138, 147, 227
FaiI YATR 8 cut(s) 21, 122, 136, 145, 185, 225, 472, 517
FalI AAGNNNNNCTT 2 cut(s) 426, 458
FatI CATG 4 cut(s) 19, 134, 143, 223
Fnu4HI GCNGC 1 cut(s) 42
FriOI GRGCYC 4 cut(s) 433, 456, 487, 495
Fsp4HI GCNGC 1 cut(s) 42
FspBI CTAG 2 cut(s) 129, 348
GlaI GCGC 1 cut(s) 113
GluI GCNGC 1 cut(s) 42
HaeII RGCGCY 1 cut(s) 115
HhaI GCGC 1 cut(s) 114
Hin1II CATG 4 cut(s) 23, 138, 147, 227
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HinfI GANTC 1 cut(s) 167
Hpy188I TCNGA 3 cut(s) 163, 277, 466
Hpy188III TCNNGA 2 cut(s) 14, 488
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 1 cut(s) 247
HpyCH4III ACNGT 3 cut(s) 91, 158, 215
HpyCH4IV ACGT 1 cut(s) 94
HpyCH4V TGCA 5 cut(s) 292, 305, 319, 395, 446
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 289
HpyF3I CTNAG 1 cut(s) 284
HpySE526I ACGT 1 cut(s) 94
Hsp92II CATG 4 cut(s) 23, 138, 147, 227
HspAI GCGC 1 cut(s) 112
LguI GCTCTTC 2 cut(s) 273, 444
LmnI GCTCC 1 cut(s) 324
LpnPI CCDG 5 cut(s) 35, 243, 278, 329, 381
Lsp1109I GCAGC 1 cut(s) 28
MaeI CTAG 2 cut(s) 129, 348
MaeII ACGT 1 cut(s) 94
MaeIII GTNAC 2 cut(s) 83, 152
MboII GAAGA 6 cut(s) 254, 290, 341, 425, 461, 515
MhlI GDGCHC 4 cut(s) 433, 456, 487, 495
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 6 cut(s) 21, 39, 150, 185, 289, 420
MwoI GCNNNNNNNGC 2 cut(s) 50, 289
NcoI CCATGG 1 cut(s) 19
NlaIII CATG 4 cut(s) 23, 138, 147, 227
NmuCI GTSAC 1 cut(s) 152
PaeR7I CTCGAG 1 cut(s) 486
PciSI GCTCTTC 2 cut(s) 273, 444
PfeI GAWTC 1 cut(s) 167
Pfl23II CGTACG 1 cut(s) 91
PkrI GCNGC 1 cut(s) 43
Psp124BI GAGCTC 3 cut(s) 456, 487, 495
PspLI CGTACG 1 cut(s) 91
PstI CTGCAG 1 cut(s) 397
RsaI GTAC 3 cut(s) 93, 180, 391
RsaNI GTAC 3 cut(s) 92, 179, 390
SacI GAGCTC 3 cut(s) 456, 487, 495
SapI GCTCTTC 2 cut(s) 273, 444
SatI GCNGC 1 cut(s) 42
SduI GDGCHC 4 cut(s) 433, 456, 487, 495
SetI ASST 8 cut(s) 97, 184, 203, 285, 382, 456, 487, 495
SfcI CTRYAG 2 cut(s) 183, 393
Sfr274I CTCGAG 1 cut(s) 486
SlaI CTCGAG 1 cut(s) 486
SmlI CTYRAG 4 cut(s) 12, 432, 455, 486
SmoI CTYRAG 4 cut(s) 12, 432, 455, 486
SpeI ACTAGT 1 cut(s) 128
SspMI CTAG 2 cut(s) 129, 348
SstI GAGCTC 3 cut(s) 456, 487, 495
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 3 cut(s) 91, 158, 215
TaiI ACGT 1 cut(s) 97
TaqI TCGA 1 cut(s) 487
TatI WGTACW 1 cut(s) 389
TfiI GAWTC 1 cut(s) 167
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 1 cut(s) 41
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 5 cut(s) 123, 180, 240, 251, 324
TspGWI ACGGA 1 cut(s) 337
XhoI CTCGAG 1 cut(s) 486
XspI CTAG 2 cut(s) 129, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.