Rorug02G0652200

Protein of unknown function (DUF1068)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0016358
Physical Location & Seq
Reverse (-)
75981580 .. 75989419
7840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0652200.1

Sequence Viewer

Length: 3105 bp
ATGGCGACCACGACTCCTCCTTTACTCTTCTTATTCTGTGTACTTTTCTCTGTCGGGATTCTAGTCGCCGACTCCAAATTTATGGTCTATAACACCTCCCAAGGGATAGTCCCCGGTAAACTTAACGTCCACTTGGTCCCTCACACCCACGATGACGTCGGATGGTTGAAGACCGTTGATCAGTATTATGTTGGCTCCAACAATTCCATCCAGGGGGCTTGTGTGCAAAATGTGTTGGACTCTTTGATTCCTGCTCTGTTGGCTGACAAGAATCGGAAATTCATCTACGTTGAAATTGCCTTTTTCCAGCGCTGGTGGAGAGATCAGAGTGATGCCGTCCAGCGTATAGTCACGGACCTTGTCAGCTCGGGTCAACTAGAATTCATAAATGGGGGCATGTGTATGCATGATGAAGCAGCTACGCATTATGTTGACATGATTGATCAGACCACTCTTGGGCATCGCTTTATTAAAAGGGAATTCGATGTCACTCCAAGAATTGGATGGCAAATTGATCCTTTTGGACATTCGGCTGTCCAGGCCTACTTGTTGGGAGCACAAGTTGGATTCGACTCACTTTACTTTGGTCGAATAGATTACCAGGACAGGGAAAAACGCAAAAATGACAAGAGTCTTGAGTTTGTTTGGCAGGGTTCTAAGAGTCTCGGTTTATCTGCTGAGATTTTTTCTGGTGCTTTCCCTGAGAATTATGAACCTCCTTCTGGTTTCTATTTTGAAGTTAATGATAAATCCCCAATAGTCCAGGATGACATCACTTTGTTTGATTACAATGTTCAAGAACGAGTTAATGACTTTGTTGCTGCTGCAGTATCACAGGCTAACATAACTCGAACAAATCATATTATGTGGACCATGGGAACAGACTTCAAGTATCAGTATGCACACACCTGGTTCCGTCAAATGGACAAGCTCATTCATTATGTCAACAAGGATGGACGTGTTAATGCTCTGTACTCTACTCCTTCCATATATACTGATGCAAAATATGCTACAAATGAATCTTGGCCAATCAAGACTGGTGACTTCTTTCCTTATGCAGATCGCATTAATGCTTACTGGACAGGATACTTCACAAGCAGACCAGCCTTGAAACACTACGTTAGAACAATGAGTGGCTATTATTTGGCTGCTAGGCAACTAGAATTTTTTAAAGGAAGGAGTACTTCAGGGCCCAATACGGACTCATTGGCAGATGCCTTAGCAATTGCTCAACATCATGATGCTGTCACTGGTACAGAAAAGCAGCACGTTGCCAATGATTATGCAAAAAGATTGTCAATAGGATACATGGAGTCTGAGCAACTAGTTGCAACTTCACTTGCTCACTTGGTTGAGTCTGCATCATATACTGGATCTGGAAACCCAACTACAAAGTTTCAACAGTGTCTACTTCTGAATATAAGCTATTGCCCTGCATCAGAAGTAACTTTGTCTCAGGGGAAAAAATTGATTGTCGTGGTGTACAATTCTCTTGGATGGAAGAGGGATGACACAGTCCGAATTCCTGTTATCAATGAAGATGTCATTGTTCATGATTCTGAGGGAAAAGCAATCGAGTCACAGCTTCTTCCTCTAGATGATGTCCATCTGGGCTTAAGGAACTACTATGTTAAGGCATACTTGGGTCAAACTCCAACAAAGACACCAAAGTATTGGCTTGTGTTTACAGTATCCGTACCACCACTTGGTTTTAGCACATACACTATCTCGGGTGCCGAAGGGGCAGGTGCTAGCTCAACCAGATCATCTGTATACACCTTTCAAAGCAGAGAAAAGTCGACTATTGAAGTTGGTCAAGGAAATGTGAAACTCTCTTTTTCCACAGATCAAGGCAAAATGACTAATTATGTCAATAGCAGAAGCTTGGTGGAAGAATCGGTTCAGCAGTCTTACAGCTTTTATAATGGGTATAATGGCAGTAATGATAAGGCTCCGCTGATTCCTCAGAATTCTGGGGCATATGTTTTCCGGCCTAATGGAACATTTCTCATAACACCCGAAGAAAAGGCTTCTTTGACTGTTATACGTGGACCAGTAATAGATGAAGTACATCAACAGATTAATTCGTGGATACATCAGATCACTAGACTTCACAAGGAAAAGGAGCATGTTGAAGTTGAGTTTATTGTTGGCCCTATACCTACTGATGATGGAATTGGAAAAGAAGTTGTAACTCAATTAGACACTGCCATGGCTACCAACAAAACGTTCTACACTGATTCTAATGGGCGTGATTTTATCAAAAGGATTCGCGACTATAGAACAGATTGGGACTTCAAAGTGAACCAACCTGTTGCTGGAAATTATTACCCAATTAATCTGGGGATTTACATGCAAGATAATAGGACAGAATTTTCAGTTTTGGTGGATCGGTCACTAGGGGGATCCAGCACAGTGGATGGTCAGATAGAGCTGATGCTTCACAGGAGACTACTACTTGATGACTCAAGAGGTGTTGCCGAGGCTCTAAATGAAACTGTCTGTGTTTCCGATGAATGCACAGGACTAAGAGTCCTAGGGAAATTTTACTTCAGAATTGACCCCTTGGGAGATGGTGCTAAGTGGCGTCGTTCTTTTGGCCAGGAGATATATTCACCTCTTCTCCTAGCCTTTGCTGAACAGGATGGAGATAACTGGAAGAATTCTCATGCAACCACTTTTTCTGGGATTGATTCCTCGTATAGTTTACCTGATAATGTTGCTCTTATAACTCTTCAGGAGCTTGATGATGGAAAAATTCTCATTCGCTTAGCACATTTATACGAGGTTGGAGAGGACAAGGATTTTTCTGTAATGGCAAATGTAGAGCTGAAGAAGCTTTTCCCCAGGAAGAAGATTGGCAAAGCGACAGAAATGAACTTATCAGCTAATCAAGAAAGGACAGAAATGGAGAGAAAGAGACTGGTATGGAAAAAGGAAGGGGGCTCGTCAGGAACGGAAAAGGGTGTGAGGGGTGGACCTGTTGATCCTGCAAAACTAGTTGTGGAACTTGCCCCAATGGAGATCCGCACCTTTCTCATAGACTTGGAGCAGAAGTTCTTCCACCGCCGCCATCATCATCATCATGATGTATCTGATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1068

Protein Analysis

1034

Amino Acids

116.68

Weight (kDa)

5.95

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 42 - 353 4.4e-83 Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid PF09261 358 - 450 3.3e-21 Alpha mannosidase middle domain
Laman-like_dom PF21260 525 - 576 5.7e-12 Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C PF07748 605 - 824 5.9e-47 Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2 PF17677 906 - 1012 1e-08 Glycosyl hydrolases family 38 C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016358)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05250
malus_domestica MD04G1210200.v1.1
prunus_persica Prupe.6G331100_v2.0.a1 Prupe.6G331100_v2.0.a1
pyrus_communis pycom04g18630
rosa_chinensis RchiOBHm_Chr3g0453981
rosa_multiflora Rmu_sc0003556.1_g000034
rosa_roxburghii Rroxscaffold_6G00430800
rosa_rugosa Rorug02G0652100 Rorug02G0652200
rosa_samantha Rh3AG054700 Rh3BG056300 Rh3CG055500 Rh3DG056800
rosa_wichuraiana Rw3G004200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1923, 2729
AarI CACCTGC 1 cut(s) 1736
AatII GACGTC 1 cut(s) 159
Acc36I ACCTGC 1 cut(s) 1736
AccB1I GGYRCC 1 cut(s) 1733
AccB7I CCANNNNNTGG 2 cut(s) 500, 1706
AccI GTMKAC 3 cut(s) 1408, 1773, 1799
AccII CGCG 1 cut(s) 2274
AciI CCGC 4 cut(s) 1955, 3028, 3067, 3070
AclI AACGTT 1 cut(s) 2228
AclWI GGATC 7 cut(s) 509, 1381, 2397, 2400, 2413, 2981, 3019
AcoI YGGCCR 2 cut(s) 1025, 2599
AcuI CTGAAG 4 cut(s) 1170, 2536, 2720, 2852
AcyI GRCGYC 2 cut(s) 156, 2587
AfaI GTAC 7 cut(s) 42, 974, 1183, 1255, 1484, 1698, 2070
AfeI AGCGCT 1 cut(s) 311
AfiI CCNNNNNNNGG 9 cut(s) 102, 213, 456, 500, 607, 722, 922, 1706, 2318
AflII CTTAAG 1 cut(s) 1615
AflIII ACRYGT 1 cut(s) 958
AhdI GACNNNNNGTC 1 cut(s) 2531
AhlI ACTAGT 2 cut(s) 1324, 2998
AjiI CACGTC 1 cut(s) 959
AjnI CCWGG 7 cut(s) 210, 537, 600, 762, 908, 2601, 2846
AjuI GAANNNNNNNTTGG 2 cut(s) 2213, 2245
AloI GAACNNNNNNTCC 2 cut(s) 3041, 3073
Alw21I GWGCWC 1 cut(s) 559
Alw26I GTCTC 4 cut(s) 668, 1458, 2443, 2914
AlwI GGATC 7 cut(s) 509, 1381, 2397, 2400, 2413, 2981, 3019
Ama87I CYCGRG 2 cut(s) 367, 1729
Aor51HI AGCGCT 1 cut(s) 311
AoxI GGCC 6 cut(s) 540, 1025, 1190, 1991, 2152, 2599
ApaI GGGCCC 1 cut(s) 1194
ApeKI GCWGC 5 cut(s) 416, 821, 824, 1148, 1264
ArsI GACNNNNNNTTYG 4 cut(s) 69, 101, 1280, 1312
AseI ATTAAT 3 cut(s) 1068, 2082, 2337
Asp700I GAANNNNTTC 3 cut(s) 1899, 2840, 3059
AspA2I CCTAGG 1 cut(s) 2536
AspLEI GCGC 1 cut(s) 312
AspS9I GGNCC 8 cut(s) 136, 355, 870, 1190, 1191, 2051, 2153, 2978
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 2 cut(s) 1052, 2607
AsuNHI GCTAGC 1 cut(s) 1751
AvaI CYCGRG 2 cut(s) 367, 1729
AvaII GGWCC 5 cut(s) 136, 355, 870, 2051, 2978
AvrII CCTAGG 1 cut(s) 2536
BaeGI GKGCMC 1 cut(s) 1194
BalI TGGCCA 2 cut(s) 1027, 2601
BamHI GGATCC 1 cut(s) 2405
BanI GGYRCC 1 cut(s) 1733
BanII GRGCYC 2 cut(s) 1194, 2948
BbsI GAAGAC 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 559
BbvI GCAGC 5 cut(s) 428, 808, 811, 1135, 1276
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 7 cut(s) 212, 539, 602, 764, 910, 2603, 2848
BciVI GTATCC 4 cut(s) 1079, 1298, 1702, 2085
BclI TGATCA 2 cut(s) 178, 442
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 4 cut(s) 668, 1458, 2443, 2914
BcuI ACTAGT 2 cut(s) 1324, 2998
BfmI CTRYAG 2 cut(s) 825, 2278
BfoI RGCGCY 1 cut(s) 313
BfrI CTTAAG 1 cut(s) 1615
BfuAI ACCTGC 1 cut(s) 1736
BfuI GTATCC 4 cut(s) 1079, 1298, 1702, 2085
BglI GCCNNNNNGGC 1 cut(s) 1742
BisI GCNGC 6 cut(s) 417, 822, 825, 1149, 1265, 3070
BlnI CCTAGG 1 cut(s) 2536
BlpI GCTNAGC 1 cut(s) 2770
BlsI GCNGC 6 cut(s) 418, 823, 826, 1150, 1266, 3071
BmcAI AGTACT 1 cut(s) 1183
Bme1390I CCNGG 8 cut(s) 114, 212, 539, 602, 764, 910, 2603, 2848
Bme18I GGWCC 5 cut(s) 136, 355, 870, 2051, 2978
BmeRI GACNNNNNGTC 1 cut(s) 2531
BmeT110I CYCGRG 2 cut(s) 367, 1729
BmgBI CACGTC 1 cut(s) 959
BmgT120I GGNCC 8 cut(s) 136, 355, 870, 1190, 1191, 2051, 2153, 2978
BmiI GGNNCC 7 cut(s) 138, 196, 914, 1192, 1735, 1953, 2407
BmrFI CCNGG 8 cut(s) 114, 212, 539, 602, 764, 910, 2603, 2848
BmsI GCATC 8 cut(s) 322, 469, 988, 1204, 1231, 1370, 1445, 2427
BmtI GCTAGC 1 cut(s) 1755
BoxI GACNNNNGTC 1 cut(s) 630
BpiI GAAGAC 1 cut(s) 176
Bpu10I CCTNAGC 1 cut(s) 1219
Bpu1102I GCTNAGC 1 cut(s) 2770
BpuEI CTTGAG 2 cut(s) 656, 2452
BpuMI CCSGG 1 cut(s) 114
BsaAI YACGTR 1 cut(s) 2048
BsaBI GATNNNNATC 1 cut(s) 1605
BsaHI GRCGYC 2 cut(s) 156, 2587
BsaJI CCNNGG 9 cut(s) 100, 112, 211, 873, 2211, 2481, 2536, 2565, 2846
BsaXI ACNNNNNCTCC 8 cut(s) 28, 31, 546, 576, 2607, 2637, 3041, 3071
Bsc4I CCNNNNNNNGG 9 cut(s) 102, 213, 456, 500, 607, 722, 922, 1706, 2318
Bse1I ACTGG 7 cut(s) 1042, 1082, 1255, 1375, 2054, 2660, 2928
Bse8I GATNNNNATC 1 cut(s) 1605
BseBI CCWGG 7 cut(s) 212, 539, 602, 764, 910, 2603, 2848
BseDI CCNNGG 9 cut(s) 100, 112, 211, 873, 2211, 2481, 2536, 2565, 2846
BseGI GGATG 9 cut(s) 167, 207, 509, 772, 958, 1502, 1513, 2425, 2650
BseJI GATNNNNATC 1 cut(s) 1605
BseLI CCNNNNNNNGG 9 cut(s) 102, 213, 456, 500, 607, 722, 922, 1706, 2318
BseMII CTCAG 6 cut(s) 669, 693, 1308, 1469, 1551, 1979
BseNI ACTGG 7 cut(s) 1042, 1082, 1255, 1375, 2054, 2660, 2928
BseRI GAGGAG 1 cut(s) 6
BseSI GKGCMC 1 cut(s) 1194
BseXI GCAGC 5 cut(s) 428, 808, 811, 1135, 1276
Bsh1236I CGCG 1 cut(s) 2274
BshFI GGCC 6 cut(s) 542, 1027, 1192, 1993, 2154, 2601
BshNI GGYRCC 1 cut(s) 1733
BsiHKAI GWGCWC 1 cut(s) 559
BsiHKCI CYCGRG 2 cut(s) 367, 1729
BsiSI CCGG 2 cut(s) 114, 1990
BslFI GGGAC 3 cut(s) 95, 122, 2306
BslI CCNNNNNNNGG 9 cut(s) 102, 213, 456, 500, 607, 722, 922, 1706, 2318
BsmAI GTCTC 4 cut(s) 668, 1458, 2443, 2914
BsmFI GGGAC 3 cut(s) 95, 122, 2306
BsmI GAATGC 1 cut(s) 2522
BsnI GGCC 6 cut(s) 542, 1027, 1192, 1993, 2154, 2601
BsoBI CYCGRG 2 cut(s) 367, 1729
Bsp120I GGGCCC 1 cut(s) 1190
Bsp1286I GDGCHC 3 cut(s) 559, 1194, 2948
Bsp1407I TGTACA 1 cut(s) 1482
Bsp1720I GCTNAGC 1 cut(s) 2770
Bsp19I CCATGG 2 cut(s) 873, 2211
Bsp68I TCGCGA 1 cut(s) 2274
BspACI CCGC 4 cut(s) 1955, 3028, 3067, 3070
BspANI GGCC 6 cut(s) 542, 1027, 1192, 1993, 2154, 2601
BspCNI CTCAG 6 cut(s) 670, 694, 1309, 1468, 1552, 1978
BspFNI CGCG 1 cut(s) 2274
BspHI TCATGA 3 cut(s) 1237, 1552, 3085
BspLI GGNNCC 7 cut(s) 138, 196, 914, 1192, 1735, 1953, 2407
BspMAI CTGCAG 1 cut(s) 829
BspMI ACCTGC 1 cut(s) 1736
BspOI GCTAGC 1 cut(s) 1755
BspPI GGATC 7 cut(s) 509, 1381, 2397, 2400, 2413, 2981, 3019
BspT107I GGYRCC 1 cut(s) 1733
BspTI CTTAAG 1 cut(s) 1615
BsrGI TGTACA 1 cut(s) 1482
BsrI ACTGG 7 cut(s) 1042, 1082, 1255, 1375, 2054, 2660, 2928
BssECI CCNNGG 9 cut(s) 100, 112, 211, 873, 2211, 2481, 2536, 2565, 2846
BssNAI GTATAC 1 cut(s) 1774
BssNI GRCGYC 2 cut(s) 156, 2587
BssT1I CCWWGG 5 cut(s) 100, 873, 2211, 2536, 2565
Bst1107I GTATAC 1 cut(s) 1774
Bst2UI CCWGG 7 cut(s) 212, 539, 602, 764, 910, 2603, 2848
Bst4CI ACNGT 7 cut(s) 175, 1404, 1516, 1690, 2041, 2416, 2500
Bst6I CTCTTC 4 cut(s) 32, 1496, 2625, 2739
BstACI GRCGYC 2 cut(s) 156, 2587
BstAFI CTTAAG 1 cut(s) 1615
BstAPI GCANNNNNTGC 1 cut(s) 1007
BstAUI TGTACA 1 cut(s) 1482
BstBAI YACGTR 1 cut(s) 2048
BstC8I GCNNGC 1 cut(s) 1753
BstDSI CCRYGG 2 cut(s) 873, 2211
BstF5I GGATG 9 cut(s) 167, 207, 509, 772, 958, 1502, 1513, 2425, 2650
BstFNI CGCG 1 cut(s) 2274
BstH2I RGCGCY 1 cut(s) 313
BstHHI GCGC 1 cut(s) 312
BstMAI GTCTC 4 cut(s) 668, 1458, 2443, 2914
BstMWI GCNNNNNNNGC 5 cut(s) 260, 539, 1007, 1742, 2836
BstNI CCWGG 7 cut(s) 212, 539, 602, 764, 910, 2603, 2848
BstNSI RCATGY 3 cut(s) 400, 2132, 2356
BstPAI GACNNNNGTC 1 cut(s) 630
BstSCI CCNGG 8 cut(s) 112, 210, 537, 600, 762, 908, 2601, 2846
BstSFI CTRYAG 2 cut(s) 825, 2278
BstSLI GKGCMC 1 cut(s) 1194
BstUI CGCG 1 cut(s) 2274
BstV1I GCAGC 5 cut(s) 428, 808, 811, 1135, 1276
BstV2I GAAGAC 1 cut(s) 176
BstX2I RGATCY 3 cut(s) 1373, 2405, 3024
BstXI CCANNNNNNTGG 3 cut(s) 82, 1674, 2416
BstYI RGATCY 3 cut(s) 1373, 2405, 3024
BstZ17I GTATAC 1 cut(s) 1774
BsuI GTATCC 4 cut(s) 1079, 1298, 1702, 2085
BsuRI GGCC 6 cut(s) 542, 1027, 1192, 1993, 2154, 2601
BtgI CCRYGG 2 cut(s) 873, 2211
BtgZI GCGATG 1 cut(s) 446
BtrI CACGTC 1 cut(s) 959
BtsCI GGATG 9 cut(s) 167, 207, 509, 772, 958, 1502, 1513, 2425, 2650
BtsI GCAGTG 1 cut(s) 2205
BtsIMutI CAGTG 5 cut(s) 1248, 1409, 2205, 2235, 2421
BtuMI TCGCGA 1 cut(s) 2274
BveI ACCTGC 1 cut(s) 1736
Cac8I GCNNGC 1 cut(s) 1753
CciI TCATGA 3 cut(s) 1237, 1552, 3085
CfoI GCGC 1 cut(s) 312
Cfr13I GGNCC 8 cut(s) 136, 355, 870, 1190, 1191, 2051, 2153, 2978
CseI GACGC 1 cut(s) 2576
CsiI ACCWGGT 1 cut(s) 908
Csp6I GTAC 7 cut(s) 41, 973, 1182, 1254, 1483, 1697, 2069
CviQI GTAC 7 cut(s) 41, 973, 1182, 1254, 1483, 1697, 2069
DraI TTTAAA 1 cut(s) 1171
DriI GACNNNNNGTC 1 cut(s) 2531
EaeI YGGCCR 2 cut(s) 1025, 2599
Eam1104I CTCTTC 4 cut(s) 32, 1496, 2625, 2739
Eam1105I GACNNNNNGTC 1 cut(s) 2531
EarI CTCTTC 4 cut(s) 32, 1496, 2625, 2739
Eco130I CCWWGG 5 cut(s) 100, 873, 2211, 2536, 2565
Eco147I AGGCCT 1 cut(s) 542
Eco24I GRGCYC 2 cut(s) 1194, 2948
Eco47I GGWCC 5 cut(s) 136, 355, 870, 2051, 2978
Eco47III AGCGCT 1 cut(s) 311
Eco57I CTGAAG 4 cut(s) 1170, 2536, 2720, 2852
Eco88I CYCGRG 2 cut(s) 367, 1729
EcoO109I RGGNCCY 1 cut(s) 1190
EcoRI GAATTC 5 cut(s) 380, 479, 1521, 1969, 2662
EcoRII CCWGG 7 cut(s) 210, 537, 600, 762, 908, 2601, 2846
EcoT14I CCWWGG 5 cut(s) 100, 873, 2211, 2536, 2565
EcoT22I ATGCAT 1 cut(s) 408
EcoT38I GRGCYC 2 cut(s) 1194, 2948
ErhI CCWWGG 5 cut(s) 100, 873, 2211, 2536, 2565
FalI AAGNNNNNCTT 4 cut(s) 1168, 1200, 1625, 1657
FaqI GGGAC 3 cut(s) 95, 122, 2306
FauNDI CATATG 1 cut(s) 1981
FbaI TGATCA 2 cut(s) 178, 442
FblI GTMKAC 3 cut(s) 1408, 1773, 1799
Fnu4HI GCNGC 6 cut(s) 417, 822, 825, 1149, 1265, 3070
FokI GGATG 9 cut(s) 174, 194, 516, 779, 965, 1509, 1520, 2432, 2657
FriOI GRGCYC 2 cut(s) 1194, 2948
Fsp4HI GCNGC 6 cut(s) 417, 822, 825, 1149, 1265, 3070
GlaI GCGC 1 cut(s) 311
GluI GCNGC 6 cut(s) 417, 822, 825, 1149, 1265, 3070
HaeII RGCGCY 1 cut(s) 313
HaeIII GGCC 6 cut(s) 542, 1027, 1192, 1993, 2154, 2601
HapII CCGG 2 cut(s) 114, 1990
HgaI GACGC 1 cut(s) 2576
HhaI GCGC 1 cut(s) 312
Hin1I GRCGYC 2 cut(s) 156, 2587
Hin6I GCGC 1 cut(s) 310
HinP1I GCGC 1 cut(s) 310
HincII GTYRAC 4 cut(s) 374, 433, 946, 1800
HindII GTYRAC 4 cut(s) 374, 433, 946, 1800
HindIII AAGCTT 2 cut(s) 1882, 2837
HpaII CCGG 2 cut(s) 114, 1990
HphI GGTGA 2 cut(s) 1052, 2607
Hpy99I CGWCG 2 cut(s) 161, 2592
HpyAV CCTTC 5 cut(s) 729, 993, 1170, 1733, 2933
HpyCH4III ACNGT 7 cut(s) 175, 1404, 1516, 1690, 2041, 2416, 2500
HpyCH4IV ACGT 8 cut(s) 126, 156, 288, 958, 1119, 1269, 2047, 2228
HpyF10VI GCNNNNNNNGC 5 cut(s) 260, 539, 1007, 1742, 2836
HpySE526I ACGT 8 cut(s) 126, 156, 288, 958, 1119, 1269, 2047, 2228
Hsp92I GRCGYC 2 cut(s) 156, 2587
HspAI GCGC 1 cut(s) 310
Ksp22I TGATCA 2 cut(s) 178, 442
LmnI GCTCC 6 cut(s) 200, 554, 1957, 2125, 2740, 3049
Lsp1109I GCAGC 5 cut(s) 428, 808, 811, 1135, 1276
LweI GCATC 8 cut(s) 322, 469, 988, 1204, 1231, 1370, 1445, 2427
MabI ACCWGGT 1 cut(s) 908
MaeII ACGT 8 cut(s) 126, 156, 288, 958, 1119, 1269, 2047, 2228
MaeIII GTNAC 8 cut(s) 349, 487, 1040, 1246, 1444, 1578, 2191, 2394
MfeI CAATTG 1 cut(s) 1224
MflI RGATCY 3 cut(s) 1373, 2405, 3024
MhlI GDGCHC 3 cut(s) 559, 1194, 2948
MlsI TGGCCA 2 cut(s) 1027, 2601
MluNI TGGCCA 2 cut(s) 1027, 2601
MmeI TCCRAC 6 cut(s) 139, 216, 222, 544, 1679, 2770
Mox20I TGGCCA 2 cut(s) 1027, 2601
Mph1103I ATGCAT 1 cut(s) 408
MroXI GAANNNNTTC 3 cut(s) 1899, 2840, 3059
MscI TGGCCA 2 cut(s) 1027, 2601
MslI CAYNNNNRTG 5 cut(s) 401, 1239, 2210, 3084, 3087
Msp20I TGGCCA 2 cut(s) 1027, 2601
MspA1I CMGCKG 1 cut(s) 1957
MspCI CTTAAG 1 cut(s) 1615
MspI CCGG 2 cut(s) 114, 1990
MspR9I CCNGG 8 cut(s) 114, 212, 539, 602, 764, 910, 2603, 2848
MunI CAATTG 1 cut(s) 1224
Mva1269I GAATGC 1 cut(s) 2522
MvaI CCWGG 7 cut(s) 212, 539, 602, 764, 910, 2603, 2848
MvnI CGCG 1 cut(s) 2274
MwoI GCNNNNNNNGC 5 cut(s) 260, 539, 1007, 1742, 2836
NciI CCSGG 1 cut(s) 114
NcoI CCATGG 2 cut(s) 873, 2211
NdeI CATATG 1 cut(s) 1981
NheI GCTAGC 1 cut(s) 1751
NlaIV GGNNCC 7 cut(s) 138, 196, 914, 1192, 1735, 1953, 2407
NmeAIII GCCGAG 1 cut(s) 2506
NmuCI GTSAC 6 cut(s) 349, 487, 1040, 1246, 1578, 2394
NruI TCGCGA 1 cut(s) 2274
NsiI ATGCAT 1 cut(s) 408
NspI RCATGY 3 cut(s) 400, 2132, 2356
PagI TCATGA 3 cut(s) 1237, 1552, 3085
PaqCI CACCTGC 1 cut(s) 1736
PceI AGGCCT 1 cut(s) 542
PctI GAATGC 1 cut(s) 2522
PdmI GAANNNNTTC 3 cut(s) 1899, 2840, 3059
PflFI GACNNNGTC 2 cut(s) 359, 1514
PflMI CCANNNNNTGG 2 cut(s) 500, 1706
PfoI TCCNGGA 1 cut(s) 762
PkrI GCNGC 6 cut(s) 418, 823, 826, 1150, 1266, 3071
Ppu21I YACGTR 1 cut(s) 2048
PshAI GACNNNNGTC 1 cut(s) 630
PshBI ATTAAT 3 cut(s) 1068, 2082, 2337
PsiI TTATAA 2 cut(s) 1923, 2729
Psp1406I AACGTT 1 cut(s) 2228
Psp6I CCWGG 7 cut(s) 210, 537, 600, 762, 908, 2601, 2846
PspGI CCWGG 7 cut(s) 210, 537, 600, 762, 908, 2601, 2846
PspN4I GGNNCC 7 cut(s) 138, 196, 914, 1192, 1735, 1953, 2407
PspOMI GGGCCC 1 cut(s) 1190
PspPI GGNCC 8 cut(s) 136, 355, 870, 1190, 1191, 2051, 2153, 2978
PstI CTGCAG 1 cut(s) 829
PsuI RGATCY 3 cut(s) 1373, 2405, 3024
PsyI GACNNNGTC 2 cut(s) 359, 1514
RruI TCGCGA 1 cut(s) 2274
RsaI GTAC 7 cut(s) 42, 974, 1183, 1255, 1484, 1698, 2070
RsaNI GTAC 7 cut(s) 41, 973, 1182, 1254, 1483, 1697, 2069
RseI CAYNNNNRTG 5 cut(s) 401, 1239, 2210, 3084, 3087
SalI GTCGAC 1 cut(s) 1798
SatI GCNGC 6 cut(s) 417, 822, 825, 1149, 1265, 3070
Sau96I GGNCC 8 cut(s) 136, 355, 870, 1190, 1191, 2051, 2153, 2978
ScaI AGTACT 1 cut(s) 1183
ScrFI CCNGG 8 cut(s) 114, 212, 539, 602, 764, 910, 2603, 2848
SduI GDGCHC 3 cut(s) 559, 1194, 2948
SexAI ACCWGGT 1 cut(s) 908
SfaNI GCATC 8 cut(s) 322, 469, 988, 1204, 1231, 1370, 1445, 2427
SfcI CTRYAG 2 cut(s) 825, 2278
SinI GGWCC 5 cut(s) 136, 355, 870, 2051, 2978
SmiMI CAYNNNNRTG 5 cut(s) 401, 1239, 2210, 3084, 3087
SmlI CTYRAG 3 cut(s) 635, 1615, 2467
SmoI CTYRAG 3 cut(s) 635, 1615, 2467
SpeI ACTAGT 2 cut(s) 1324, 2998
SseBI AGGCCT 1 cut(s) 542
SsiI CCGC 4 cut(s) 1955, 3028, 3067, 3070
StuI AGGCCT 1 cut(s) 542
StyD4I CCNGG 8 cut(s) 112, 210, 537, 600, 762, 908, 2601, 2846
StyI CCWWGG 5 cut(s) 100, 873, 2211, 2536, 2565
TaaI ACNGT 7 cut(s) 175, 1404, 1516, 1690, 2041, 2416, 2500
TaiI ACGT 8 cut(s) 129, 159, 291, 961, 1122, 1272, 2050, 2231
TaqI TCGA 6 cut(s) 483, 570, 589, 850, 1575, 1799
TaqII GACCGA 1 cut(s) 2382
TatI WGTACW 5 cut(s) 40, 972, 1181, 1482, 2068
TauI GCSGC 1 cut(s) 3072
TscAI CASTG 5 cut(s) 1255, 1409, 2212, 2242, 2421
TseFI GTSAC 6 cut(s) 349, 487, 1040, 1246, 1578, 2394
TseI GCWGC 5 cut(s) 416, 821, 824, 1148, 1264
Tsp45I GTSAC 6 cut(s) 349, 487, 1040, 1246, 1578, 2394
TspGWI ACGGA 5 cut(s) 368, 905, 1214, 1684, 2972
TspRI CASTG 5 cut(s) 1255, 1409, 2212, 2242, 2421
Tth111I GACNNNGTC 2 cut(s) 359, 1514
Van91I CCANNNNNTGG 2 cut(s) 500, 1706
Vha464I CTTAAG 1 cut(s) 1615
VpaK11BI GGWCC 5 cut(s) 136, 355, 870, 2051, 2978
VspI ATTAAT 3 cut(s) 1068, 2082, 2337
XbaI TCTAGA 1 cut(s) 1594
XceI RCATGY 3 cut(s) 400, 2132, 2356
XcmI CCANNNNNNNNNTGG 2 cut(s) 501, 2315
XmaJI CCTAGG 1 cut(s) 2536
XmiI GTMKAC 3 cut(s) 1408, 1773, 1799
XmnI GAANNNNTTC 3 cut(s) 1899, 2840, 3059
ZraI GACGTC 1 cut(s) 157
ZrmI AGTACT 1 cut(s) 1183
Zsp2I ATGCAT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.