MD04G1210200.v1.1

Protein of unknown function (DUF1068)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016358
Physical Location & Seq
Reverse (-)
29482499 .. 29484120
1622 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1210200.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGCTCATCCGACAAAACATGGGATTTGCAGTAATGGTGTTCTGAGGCTGGTTTTTGTGTTAGTAGGTTTGTGCCTGGTCGGGTACGCAGTCGTACATCCAACGTTGTACTGGAGCTTTAAGGAGCACAGTTCGTGTCCGCAATGTCCTTGTGATTGTTCAACGGATTTCGCTTCCGCACCTGTAGACTGCGGAAAACATGAACCAGAAATGAAAGAGGAGATGGAGAAGGACATCATCGCCTTGATGTCGGAAGAGCTAAGTTTGCAGCAAGCCGTAGCCAACGAAACCCTGCAACGGGCCGATGCAGCGTTAATGGGAGTTAGGAAAGTCTCTTCACTCTTCCAAAAGGAGGCAGAAAAATGTAATGTAGGAGTGGAAACTTGTGAAGAAGCGAGGGAGAGGGCAGAAGCAGAACTTGCAGAAGAGCTTAGGCTTTCGGAAGTATGGGAGAAACGAGCTCAGGAGCTCGGGTGGAACGATAAAAGAGCATACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1068

Protein Analysis

167

Amino Acids

18.55

Weight (kDa)

5.0

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1068 PF06364 13 - 161 1.4e-47 Protein of unknown function (DUF1068)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016358)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05250
malus_domestica MD04G1210200.v1.1
prunus_persica Prupe.6G331100_v2.0.a1 Prupe.6G331100_v2.0.a1
pyrus_communis pycom04g18630
rosa_chinensis RchiOBHm_Chr3g0453981
rosa_multiflora Rmu_sc0003556.1_g000034
rosa_roxburghii Rroxscaffold_6G00430800
rosa_rugosa Rorug02G0652100 Rorug02G0652200
rosa_samantha Rh3AG054700 Rh3BG056300 Rh3CG055500 Rh3DG056800
rosa_wichuraiana Rw3G004200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 186
AciI CCGC 3 cut(s) 140, 177, 192
AclI AACGTT 1 cut(s) 104
AfaI GTAC 3 cut(s) 86, 96, 110
AfiI CCNNNNNNNGG 3 cut(s) 297, 298, 352
AgsI TTSAA 1 cut(s) 162
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 5 cut(s) 117, 259, 430, 461, 469
AluI AGCT 5 cut(s) 117, 259, 430, 461, 469
Alw21I GWGCWC 3 cut(s) 129, 463, 471
Alw26I GTCTC 1 cut(s) 337
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 2 cut(s) 268, 308
AspS9I GGNCC 1 cut(s) 300
AvaI CYCGRG 1 cut(s) 470
BanII GRGCYC 2 cut(s) 463, 471
Bbv12I GWGCWC 3 cut(s) 129, 463, 471
BbvI GCAGC 2 cut(s) 280, 320
BccI CCATC 1 cut(s) 217
BceAI ACGGC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 77
BcoDI GTCTC 1 cut(s) 337
BfmI CTRYAG 1 cut(s) 183
BisI GCNGC 2 cut(s) 269, 309
BlsI GCNGC 2 cut(s) 270, 310
Bme1390I CCNGG 1 cut(s) 77
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 300
BmrFI CCNGG 1 cut(s) 77
BmsI GCATC 1 cut(s) 295
BpmI CTGGAG 1 cut(s) 133
Bpu10I CCTNAGC 2 cut(s) 431, 462
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 3 cut(s) 297, 298, 352
Bse1I ACTGG 1 cut(s) 116
Bse3DI GCAATG 1 cut(s) 149
BseBI CCWGG 1 cut(s) 77
BseGI GGATG 2 cut(s) 7, 97
BseLI CCNNNNNNNGG 3 cut(s) 297, 298, 352
BseMI GCAATG 1 cut(s) 149
BseMII CTCAG 2 cut(s) 35, 476
BseNI ACTGG 1 cut(s) 116
BseRI GAGGAG 1 cut(s) 233
BseXI GCAGC 2 cut(s) 280, 320
BshFI GGCC 1 cut(s) 302
BsiHKAI GWGCWC 3 cut(s) 129, 463, 471
BsiHKCI CYCGRG 1 cut(s) 470
BslI CCNNNNNNNGG 3 cut(s) 297, 298, 352
BsmAI GTCTC 1 cut(s) 337
BsnI GGCC 1 cut(s) 302
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 3 cut(s) 129, 463, 471
BspACI CCGC 3 cut(s) 140, 177, 192
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 2 cut(s) 36, 475
BspQI GCTCTTC 2 cut(s) 249, 420
BsrDI GCAATG 1 cut(s) 149
BsrI ACTGG 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 4 cut(s) 249, 340, 347, 420
BstAPI GCANNNNNTGC 1 cut(s) 419
BstC8I GCNNGC 1 cut(s) 273
BstDEI CTNAG 4 cut(s) 44, 260, 431, 462
BstF5I GGATG 2 cut(s) 7, 97
BstMAI GTCTC 1 cut(s) 337
BstMWI GCNNNNNNNGC 3 cut(s) 265, 308, 419
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 75
BstSFI CTRYAG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 280, 320
BsuRI GGCC 1 cut(s) 302
BtgZI GCGATG 1 cut(s) 223
BtsCI GGATG 2 cut(s) 7, 97
Cac8I GCNNGC 1 cut(s) 273
Cfr13I GGNCC 1 cut(s) 300
Csp6I GTAC 3 cut(s) 85, 95, 109
CviAII CATG 2 cut(s) 20, 200
CviQI GTAC 3 cut(s) 85, 95, 109
DdeI CTNAG 4 cut(s) 44, 260, 431, 462
Eam1104I CTCTTC 4 cut(s) 249, 340, 347, 420
EarI CTCTTC 4 cut(s) 249, 340, 347, 420
Ecl136II GAGCTC 2 cut(s) 461, 469
Eco24I GRGCYC 2 cut(s) 463, 471
Eco53kI GAGCTC 2 cut(s) 461, 469
Eco88I CYCGRG 1 cut(s) 470
EcoICRI GAGCTC 2 cut(s) 461, 469
EcoRII CCWGG 1 cut(s) 75
EcoT38I GRGCYC 2 cut(s) 463, 471
FaeI CATG 2 cut(s) 23, 203
FaiI YATR 4 cut(s) 21, 201, 448, 493
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FatI CATG 2 cut(s) 19, 199
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 2 cut(s) 269, 309
FokI GGATG 1 cut(s) 84
FriOI GRGCYC 2 cut(s) 463, 471
Fsp4HI GCNGC 2 cut(s) 269, 309
GluI GCNGC 2 cut(s) 269, 309
GsuI CTGGAG 1 cut(s) 133
HaeIII GGCC 1 cut(s) 302
Hin1II CATG 2 cut(s) 23, 203
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 4 cut(s) 12, 45, 253, 442
Hpy188III TCNNGA 2 cut(s) 464, 498
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 1 cut(s) 223
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 1 cut(s) 104
HpyCH4V TGCA 5 cut(s) 30, 268, 295, 308, 422
HpyF10VI GCNNNNNNNGC 3 cut(s) 265, 308, 419
HpyF3I CTNAG 4 cut(s) 44, 260, 431, 462
HpySE526I ACGT 1 cut(s) 104
Hsp92II CATG 2 cut(s) 23, 203
LguI GCTCTTC 2 cut(s) 249, 420
LmnI GCTCC 3 cut(s) 114, 124, 466
LpnPI CCDG 8 cut(s) 35, 62, 89, 97, 195, 219, 305, 449
Lsp1109I GCAGC 2 cut(s) 280, 320
LweI GCATC 1 cut(s) 295
MaeII ACGT 1 cut(s) 104
MboII GAAGA 5 cut(s) 266, 327, 334, 401, 437
MhlI GDGCHC 3 cut(s) 129, 463, 471
MmeI TCCRAC 3 cut(s) 35, 125, 231
MnlI CCTC 5 cut(s) 39, 211, 346, 390, 396
MseI TTAA 2 cut(s) 120, 314
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
MwoI GCNNNNNNNGC 3 cut(s) 265, 308, 419
NlaIII CATG 2 cut(s) 23, 203
PciSI GCTCTTC 2 cut(s) 249, 420
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PkrI GCNGC 2 cut(s) 270, 310
Psp124BI GAGCTC 2 cut(s) 463, 471
Psp1406I AACGTT 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspPI GGNCC 1 cut(s) 300
RsaI GTAC 3 cut(s) 86, 96, 110
RsaNI GTAC 3 cut(s) 85, 95, 109
SacI GAGCTC 2 cut(s) 463, 471
SapI GCTCTTC 2 cut(s) 249, 420
SaqAI TTAA 2 cut(s) 120, 314
SatI GCNGC 2 cut(s) 269, 309
Sau96I GGNCC 1 cut(s) 300
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 3 cut(s) 129, 463, 471
SetI ASST 8 cut(s) 70, 107, 119, 184, 261, 432, 463, 471
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 1 cut(s) 183
SsiI CCGC 3 cut(s) 140, 177, 192
SstI GAGCTC 2 cut(s) 463, 471
StyD4I CCNGG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 1 cut(s) 107
TatI WGTACW 1 cut(s) 108
Tru1I TTAA 2 cut(s) 120, 314
Tru9I TTAA 2 cut(s) 120, 314
TseI GCWGC 2 cut(s) 268, 308
TspDTI ATGAA 2 cut(s) 216, 227
TspGWI ACGGA 1 cut(s) 179
XcmI CCANNNNNNNNNTGG 1 cut(s) 108
XmiI GTMKAC 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.