Rroxscaffold_6G00430800

Protein of unknown function (DUF1068)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0016358
Physical Location & Seq
Forward (+)
50903879 .. 50905403
1525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430800.1

Sequence Viewer

Length: 510 bp
ATGGCTCATCCACAAGACCATGGCTTTTGTTCTAACAGTGCTGTGAGGCTGGCTGTGATTATGATAGGTGTGTGTTTGGTTGGTTACACCGTACGTCAATCATTTTATTGGCGCTTTAAGGAGAGTAGTTCATGCCCTTCATGTCACTGTGACTGTTCAAAGGACTCTGTCCTCATACCTATAGATTGTGGTAAACACGAACCAGAAATGAAAGAGGAAATGGAGAAGGATATCATAGCATTGATGTCCGAAGAGCTAAGTTTGCAGCAGACTGTTGCCAATGAAACCTTGCAACAGAGTGAAGCACTGTTGATGAGTGCTAGGAAAGTCTCTTCACACTACCAAAAAGAAGCAGAAAAATGCACTGCTGGAGTGGAAACTTGTGAAGAAGCAAGGAGAGGGCTCAAGCGCAACTTGCAGAAGAGCTTAAGGCTTCAGAAGTGTGGGAGAGACGAGCTCGGGAGCTTGGGTGGGAAGATACGAGATTATATTGATGAACGTACTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1068

Protein Analysis

169

Amino Acids

19.02

Weight (kDa)

6.22

Isoelectric Point (pI)

67.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1068 PF06364 13 - 145 6.9e-38 Protein of unknown function (DUF1068)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016358)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05250
malus_domestica MD04G1210200.v1.1
prunus_persica Prupe.6G331100_v2.0.a1 Prupe.6G331100_v2.0.a1
pyrus_communis pycom04g18630
rosa_chinensis RchiOBHm_Chr3g0453981
rosa_multiflora Rmu_sc0003556.1_g000034
rosa_roxburghii Rroxscaffold_6G00430800
rosa_rugosa Rorug02G0652100 Rorug02G0652200
rosa_samantha Rh3AG054700 Rh3BG056300 Rh3CG055500 Rh3DG056800
rosa_wichuraiana Rw3G004200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 419
AfaI GTAC 2 cut(s) 93, 502
AflII CTTAAG 1 cut(s) 427
AgsI TTSAA 1 cut(s) 159
AluBI AGCT 4 cut(s) 256, 426, 457, 465
AluI AGCT 4 cut(s) 256, 426, 457, 465
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 2 cut(s) 334, 444
Ama87I CYCGRG 1 cut(s) 458
ApeKI GCWGC 1 cut(s) 265
AspLEI GCGC 2 cut(s) 114, 411
AvaI CYCGRG 1 cut(s) 458
BanII GRGCYC 2 cut(s) 405, 459
Bbv12I GWGCWC 1 cut(s) 459
BbvI GCAGC 1 cut(s) 277
BcoDI GTCTC 2 cut(s) 334, 444
BfaI CTAG 1 cut(s) 321
BfmI CTRYAG 1 cut(s) 180
BfoI RGCGCY 1 cut(s) 115
BfrI CTTAAG 1 cut(s) 427
BisI GCNGC 1 cut(s) 266
BlsI GCNGC 1 cut(s) 267
BmeT110I CYCGRG 1 cut(s) 458
BplI GAGNNNNNCTC 2 cut(s) 441, 473
BpmI CTGGAG 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 19
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
BseDI CCNNGG 1 cut(s) 19
BseGI GGATG 1 cut(s) 7
BseXI GCAGC 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 459
BsiHKCI CYCGRG 1 cut(s) 458
BsiWI CGTACG 1 cut(s) 91
BsmAI GTCTC 2 cut(s) 334, 444
BsmBI CGTCTC 1 cut(s) 444
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 2 cut(s) 405, 459
Bsp19I CCATGG 1 cut(s) 19
BspQI GCTCTTC 2 cut(s) 246, 416
BspTI CTTAAG 1 cut(s) 427
BssECI CCNNGG 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 19
Bst4CI ACNGT 6 cut(s) 38, 91, 149, 155, 274, 309
Bst6I CTCTTC 3 cut(s) 246, 337, 416
BstAFI CTTAAG 1 cut(s) 427
BstC8I GCNNGC 1 cut(s) 51
BstDEI CTNAG 1 cut(s) 257
BstDSI CCRYGG 1 cut(s) 19
BstF5I GGATG 1 cut(s) 7
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 2 cut(s) 114, 411
BstMAI GTCTC 2 cut(s) 334, 444
BstMWI GCNNNNNNNGC 2 cut(s) 262, 415
BstSFI CTRYAG 1 cut(s) 180
BstV1I GCAGC 1 cut(s) 277
BtgI CCRYGG 1 cut(s) 19
BtsCI GGATG 1 cut(s) 7
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 4 cut(s) 43, 145, 305, 363
Cac8I GCNNGC 1 cut(s) 51
CfoI GCGC 2 cut(s) 114, 411
Csp6I GTAC 2 cut(s) 92, 501
CviAII CATG 3 cut(s) 20, 132, 141
CviQI GTAC 2 cut(s) 92, 501
DdeI CTNAG 1 cut(s) 257
Eam1104I CTCTTC 3 cut(s) 246, 337, 416
EarI CTCTTC 3 cut(s) 246, 337, 416
Ecl136II GAGCTC 1 cut(s) 457
Eco130I CCWWGG 1 cut(s) 19
Eco24I GRGCYC 2 cut(s) 405, 459
Eco32I GATATC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 419
Eco88I CYCGRG 1 cut(s) 458
EcoICRI GAGCTC 1 cut(s) 457
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 19
EcoT38I GRGCYC 2 cut(s) 405, 459
ErhI CCWWGG 1 cut(s) 19
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 3 cut(s) 23, 135, 144
FaiI YATR 8 cut(s) 21, 62, 133, 142, 176, 182, 236, 489
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FatI CATG 3 cut(s) 19, 131, 140
Fnu4HI GCNGC 1 cut(s) 266
FriOI GRGCYC 2 cut(s) 405, 459
Fsp4HI GCNGC 1 cut(s) 266
FspBI CTAG 1 cut(s) 321
GlaI GCGC 2 cut(s) 113, 410
GluI GCNGC 1 cut(s) 266
GsuI CTGGAG 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 115
HhaI GCGC 2 cut(s) 114, 411
Hin1II CATG 3 cut(s) 23, 135, 144
Hin6I GCGC 2 cut(s) 112, 409
HinP1I GCGC 2 cut(s) 112, 409
HinfI GANTC 1 cut(s) 164
Hpy166II GTNNAC 1 cut(s) 194
Hpy188I TCNGA 2 cut(s) 250, 438
Hpy188III TCNNGA 1 cut(s) 460
Hpy8I GTNNAC 1 cut(s) 194
HpyAV CCTTC 2 cut(s) 147, 220
HpyCH4III ACNGT 6 cut(s) 38, 91, 149, 155, 274, 309
HpyCH4IV ACGT 2 cut(s) 94, 499
HpyCH4V TGCA 4 cut(s) 265, 292, 363, 418
HpyF10VI GCNNNNNNNGC 2 cut(s) 262, 415
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 2 cut(s) 94, 499
Hsp92II CATG 3 cut(s) 23, 135, 144
HspAI GCGC 2 cut(s) 112, 409
LguI GCTCTTC 2 cut(s) 246, 416
LmnI GCTCC 1 cut(s) 462
LpnPI CCDG 3 cut(s) 35, 216, 354
Lsp1109I GCAGC 1 cut(s) 277
MaeI CTAG 1 cut(s) 321
MaeII ACGT 2 cut(s) 94, 499
MaeIII GTNAC 3 cut(s) 83, 143, 149
MboII GAAGA 5 cut(s) 263, 324, 398, 433, 487
MhlI GDGCHC 2 cut(s) 405, 459
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 4 cut(s) 39, 182, 208, 392
MseI TTAA 3 cut(s) 117, 428, 508
MspCI CTTAAG 1 cut(s) 427
MwoI GCNNNNNNNGC 2 cut(s) 262, 415
NcoI CCATGG 1 cut(s) 19
NlaIII CATG 3 cut(s) 23, 135, 144
NmuCI GTSAC 2 cut(s) 143, 149
PciSI GCTCTTC 2 cut(s) 246, 416
Pfl23II CGTACG 1 cut(s) 91
PflFI GACNNNGTC 1 cut(s) 167
PkrI GCNGC 1 cut(s) 267
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Psp124BI GAGCTC 1 cut(s) 459
PspLI CGTACG 1 cut(s) 91
PsyI GACNNNGTC 1 cut(s) 167
RsaI GTAC 2 cut(s) 93, 502
RsaNI GTAC 2 cut(s) 92, 501
SacI GAGCTC 1 cut(s) 459
SapI GCTCTTC 2 cut(s) 246, 416
SaqAI TTAA 3 cut(s) 117, 428, 508
SatI GCNGC 1 cut(s) 266
SchI GAGTC 1 cut(s) 158
SduI GDGCHC 2 cut(s) 405, 459
SetI ASST 9 cut(s) 70, 97, 181, 258, 290, 428, 459, 467, 502
SfcI CTRYAG 1 cut(s) 180
SmlI CTYRAG 2 cut(s) 404, 427
SmoI CTYRAG 2 cut(s) 404, 427
SspMI CTAG 1 cut(s) 321
SstI GAGCTC 1 cut(s) 459
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 6 cut(s) 38, 91, 149, 155, 274, 309
TaiI ACGT 2 cut(s) 97, 502
Tru1I TTAA 3 cut(s) 117, 428, 508
Tru9I TTAA 3 cut(s) 117, 428, 508
TscAI CASTG 4 cut(s) 43, 152, 312, 370
TseFI GTSAC 2 cut(s) 143, 149
TseI GCWGC 1 cut(s) 265
Tsp45I GTSAC 2 cut(s) 143, 149
TspDTI ATGAA 5 cut(s) 120, 129, 224, 297, 510
TspRI CASTG 4 cut(s) 43, 152, 312, 370
Tth111I GACNNNGTC 1 cut(s) 167
Vha464I CTTAAG 1 cut(s) 427
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.