FvH4_6g06010

Protein AUXIN RESPONSE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011194
Physical Location & Seq
Forward (+)
3432843 .. 3436041
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g06010.t1

Sequence Viewer

Length: 1440 bp
ATGGTGATCATCACAGAGGAAGAAGACAACCAAAAACCACACCCAAAACCCTCCCCAAATCCCAAACCCACCTCTTCTTCCAAACCCTCTCCACCATCAAAACCACCCCAAAACGTTCCAAACCCATTCGCCTTCTGGTTCTACTTCACCATCACAGTCTCCATCATCACCTTCTTCTTCATCTCCTTCTCCTCTCTCTCCCCGCAAGACCCAAAGTCGTGGTTTTTAAGCTTACCCACGTCGCTGCGTCAGCACTACTCCAAGGGCCGCACCGTAAAAGTCCAGACCCACCCGGACCAGTCCCCGTTTGAAGTCTTCACAGCTGAGAAAGGCGTCACGGAATCGGAGAAGGTTATAATCGTCCATGGGTTGGGGCTCAGTTCGTTTGGTTTTCGCAAGATAATTGAGGCTTTGGGTGCGAGAGGGGTTCATGTTGTGGCGTTTGATTTGCCCGGAAATGGGTTTTCGGATAAGTCGAAGGAGGAGATTGAGGAGAACCCAGGTGGGTTTTTTGGGAGGTTGAGGTTTGTGGTTAGTGAAATTCAAGAAAAGGGGCTGTTTTGGGCGTTTGATGAGATTGTGGCTACTGGGCAGGTGCCGTTTGAGGAGATTGAGGCTCGGGCGATGAAGAGGAAGGTGGTGAAGCCGATTGAGTTGGGGCCTGGTGAGATTGGGAAGGTGTTGGGGCAAGTGATTGAGACAATGGGGTTGGCTCCTGTGCATTTGGTTCTGCATGATTCGGCTTTGGGGATGGTTTCGAATTGGGTTTTGGAGAATGTGAAGTCTGTGAGGAGTGTGACATTGATTGATACAGGGTCGAAATCAGTAGGGGCTTTGCCCTTGTGGGCTTTGGAAGTGCCGTTGGTTAGGGAGTTGGTGTTGGGGGTTTCGTATGCTCATGCATTGTTGATTAGGATGAGTTGTTCGAGAGGGGTTGGTGGTGCGGATTTGGATGCACATAGAGTTCTTTTGAAAGGGAGGGATGGGACGAAGGCGATTGTTGGAATGGGGAAGAAGTTGAATCATAGCTTTGATATTGCAGAATGGGGTGGTTCAGATGGACTGAAAGGAGTGCCAATGCAGGTTCTTTGGTCTGGAAGTTGGTCTAAGGAATGGAGTGAAGAGGGACACCGAGTTGCGAATGCACTTCCAAAGGCAACTTTTGTCACCCATTCTGGTGGAAGGTGGCCTCAGGAGGATGCAGCCGATGAAGTAGCTGGACACATTGCAAATTTTGTAACCTCGTTACCTGCTACTGTCAGAAAGGTCCAGGAAGAGCCGATCCCTGAGCGTGTTCAGAAGTTGCTCGATGAAGCCGAACAAAGTGGTCATCACGGACATGTTCATGGCCATGGGCATGAAGATCATAGTCATAATGTTCATGGCGCTGGAGCTGGCTATCCTGATCCGTACGGCCTTGGTCATGGTCACGGATGGTGA

Protein Analysis

480

Amino Acids

52.27

Weight (kDa)

6.39

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 118 - 273 1.1e-10 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 118 - 406 3.3e-07 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54990
fragaria_vesca FvH4_6g06010
malus_domestica MD04G1204900.v1.1 MD12G1218600.v1.1
prunus_persica Prupe.6G324900_v2.0.a1
pyrus_communis pycom04g18110 pycom12g20410
rosa_chinensis RchiOBHm_Chr3g0454971
rosa_laevigata RLG00000025397
rosa_multiflora Rmu_sc0002169.1_g000009
rosa_roxburghii Rroxscaffold_6G00424640
rosa_rugosa Rorug03G0003400 Rorug03G0003500 Rorug03G0003500 Rorug03G0003600
rosa_samantha Rh3AG063200 Rh3BG065200 Rh3CG064000 Rh3DG065200
rosa_wichuraiana Rw3G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 356
AarI CACCTGC 1 cut(s) 583
Acc36I ACCTGC 3 cut(s) 583, 1072, 1258
AccB1I GGYRCC 1 cut(s) 595
AccB7I CCANNNNNTGG 1 cut(s) 370
AciI CCGC 3 cut(s) 203, 268, 944
AclI AACGTT 1 cut(s) 114
AclWI GGATC 2 cut(s) 1276, 1400
AcoI YGGCCR 1 cut(s) 1348
AcsI RAATTY 2 cut(s) 540, 1231
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 1 cut(s) 1412
AfiI CCNNNNNNNGG 3 cut(s) 370, 458, 459
AflIII ACRYGT 1 cut(s) 1339
AgsI TTSAA 4 cut(s) 311, 545, 973, 1021
AhdI GACNNNNNGTC 1 cut(s) 214
AjiI CACGTC 1 cut(s) 240
AjnI CCWGG 3 cut(s) 499, 661, 1269
AjuI GAANNNNNNNTTGG 2 cut(s) 752, 784
AloI GAACNNNNNNTCC 2 cut(s) 907, 939
AluBI AGCT 5 cut(s) 231, 323, 1029, 1217, 1394
AluI AGCT 5 cut(s) 231, 323, 1029, 1217, 1394
Alw26I GTCTC 2 cut(s) 163, 692
AlwI GGATC 2 cut(s) 1276, 1400
Ama87I CYCGRG 1 cut(s) 618
AoxI GGCC 5 cut(s) 265, 659, 1187, 1348, 1414
ApeKI GCWGC 2 cut(s) 244, 1202
ApoI RAATTY 2 cut(s) 540, 1231
AspLEI GCGC 1 cut(s) 1388
AspS9I GGNCC 4 cut(s) 265, 295, 659, 1267
AsuC2I CCSGG 2 cut(s) 293, 453
AsuHPI GGTGA 6 cut(s) 16, 139, 160, 652, 677, 1159
AsuII TTCGAA 1 cut(s) 758
AvaI CYCGRG 1 cut(s) 618
AvaII GGWCC 2 cut(s) 295, 1267
AxyI CCTNAGG 1 cut(s) 1191
BalI TGGCCA 1 cut(s) 1350
BanI GGYRCC 1 cut(s) 595
BanII GRGCYC 1 cut(s) 378
BbsI GAAGAC 2 cut(s) 30, 307
BbvI GCAGC 2 cut(s) 231, 1214
BccI CCATC 7 cut(s) 103, 158, 170, 745, 977, 1052, 1428
BceAI ACGGC 3 cut(s) 583, 844, 1429
BciT130I CCWGG 3 cut(s) 501, 663, 1271
BclI TGATCA 1 cut(s) 6
BcnI CCSGG 2 cut(s) 293, 453
BcoDI GTCTC 2 cut(s) 163, 692
BfoI RGCGCY 1 cut(s) 1389
BfuAI ACCTGC 3 cut(s) 583, 1072, 1258
BisI GCNGC 3 cut(s) 245, 268, 1203
BlsI GCNGC 3 cut(s) 246, 269, 1204
Bme1390I CCNGG 5 cut(s) 293, 453, 501, 663, 1271
Bme18I GGWCC 2 cut(s) 295, 1267
BmeRI GACNNNNNGTC 1 cut(s) 214
BmeT110I CYCGRG 1 cut(s) 618
BmgBI CACGTC 1 cut(s) 240
BmgT120I GGNCC 4 cut(s) 265, 295, 659, 1267
BmiI GGNNCC 3 cut(s) 597, 660, 714
BmrFI CCNGG 5 cut(s) 293, 453, 501, 663, 1271
BmrI ACTGGG 1 cut(s) 597
BmsI GCATC 2 cut(s) 943, 1189
BmuI ACTGGG 1 cut(s) 597
BpiI GAAGAC 2 cut(s) 30, 307
BpmI CTGGAG 1 cut(s) 1410
Bpu10I CCTNAGC 1 cut(s) 1287
Bpu14I TTCGAA 1 cut(s) 758
BpuMI CCSGG 2 cut(s) 293, 453
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 5 cut(s) 261, 364, 499, 1351, 1417
BsaXI ACNNNNNCTCC 2 cut(s) 863, 893
Bsc4I CCNNNNNNNGG 3 cut(s) 370, 458, 459
Bse1I ACTGG 2 cut(s) 298, 592
Bse21I CCTNAGG 1 cut(s) 1191
Bse3DI GCAATG 1 cut(s) 1224
BseBI CCWGG 3 cut(s) 501, 663, 1271
BseDI CCNNGG 5 cut(s) 261, 364, 499, 1351, 1417
BseGI GGATG 6 cut(s) 756, 921, 958, 988, 1204, 1439
BseLI CCNNNNNNNGG 3 cut(s) 370, 458, 459
BseMI GCAATG 1 cut(s) 1224
BseMII CTCAG 4 cut(s) 315, 391, 1205, 1278
BseNI ACTGG 2 cut(s) 298, 592
BseRI GAGGAG 5 cut(s) 181, 497, 506, 620, 805
BseXI GCAGC 2 cut(s) 231, 1214
BshFI GGCC 5 cut(s) 267, 661, 1189, 1350, 1416
BshNI GGYRCC 1 cut(s) 595
BsiHKCI CYCGRG 1 cut(s) 618
BsiSI CCGG 2 cut(s) 293, 453
BsiWI CGTACG 1 cut(s) 1410
BslFI GGGAC 3 cut(s) 286, 1000, 1140
BslI CCNNNNNNNGG 3 cut(s) 370, 458, 459
BsmAI GTCTC 2 cut(s) 163, 692
BsmFI GGGAC 3 cut(s) 286, 1000, 1140
BsmI GAATGC 1 cut(s) 1147
BsnI GGCC 5 cut(s) 267, 661, 1189, 1350, 1416
BsoBI CYCGRG 1 cut(s) 618
Bsp119I TTCGAA 1 cut(s) 758
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 4 cut(s) 6, 1281, 1363, 1405
Bsp19I CCATGG 2 cut(s) 364, 1351
BspACI CCGC 3 cut(s) 203, 268, 944
BspANI GGCC 5 cut(s) 267, 661, 1189, 1350, 1416
BspCNI CTCAG 4 cut(s) 316, 390, 1204, 1279
BspLI GGNNCC 3 cut(s) 597, 660, 714
BspMI ACCTGC 3 cut(s) 583, 1072, 1258
BspPI GGATC 2 cut(s) 1276, 1400
BspQI GCTCTTC 1 cut(s) 1269
BspT104I TTCGAA 1 cut(s) 758
BspT107I GGYRCC 1 cut(s) 595
BsrDI GCAATG 1 cut(s) 1224
BsrI ACTGG 2 cut(s) 298, 592
BssECI CCNNGG 5 cut(s) 261, 364, 499, 1351, 1417
BssMI GATC 4 cut(s) 6, 1281, 1363, 1405
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 4 cut(s) 261, 364, 1351, 1417
Bst2UI CCWGG 3 cut(s) 501, 663, 1271
Bst4CI ACNGT 3 cut(s) 157, 274, 1258
Bst6I CTCTTC 4 cut(s) 79, 623, 1116, 1269
BstACI GRCGYC 1 cut(s) 333
BstBI TTCGAA 1 cut(s) 758
BstC8I GCNNGC 1 cut(s) 1396
BstDEI CTNAG 5 cut(s) 324, 377, 1107, 1191, 1287
BstDSI CCRYGG 2 cut(s) 364, 1351
BstF5I GGATG 6 cut(s) 756, 921, 958, 988, 1204, 1439
BstH2I RGCGCY 1 cut(s) 1389
BstHHI GCGC 1 cut(s) 1388
BstKTI GATC 4 cut(s) 9, 1284, 1366, 1408
BstMAI GTCTC 2 cut(s) 163, 692
BstMBI GATC 4 cut(s) 6, 1281, 1363, 1405
BstMWI GCNNNNNNNGC 2 cut(s) 250, 416
BstNI CCWGG 3 cut(s) 501, 663, 1271
BstNSI RCATGY 1 cut(s) 1343
BstSCI CCNGG 5 cut(s) 291, 451, 499, 661, 1269
BstV1I GCAGC 2 cut(s) 231, 1214
BstV2I GAAGAC 2 cut(s) 30, 307
BstXI CCANNNNNNTGG 2 cut(s) 219, 1178
Bsu36I CCTNAGG 1 cut(s) 1191
BsuRI GGCC 5 cut(s) 267, 661, 1189, 1350, 1416
BtgI CCRYGG 2 cut(s) 364, 1351
BtgZI GCGATG 1 cut(s) 638
BtrI CACGTC 1 cut(s) 240
BtsCI GGATG 6 cut(s) 756, 921, 958, 988, 1204, 1439
BveI ACCTGC 3 cut(s) 583, 1072, 1258
Cac8I GCNNGC 1 cut(s) 1396
CfoI GCGC 1 cut(s) 1388
Cfr13I GGNCC 4 cut(s) 265, 295, 659, 1267
CseI GACGC 2 cut(s) 236, 322
Csp6I GTAC 1 cut(s) 1411
CviQI GTAC 1 cut(s) 1411
DdeI CTNAG 5 cut(s) 324, 377, 1107, 1191, 1287
DpnI GATC 4 cut(s) 8, 1283, 1365, 1407
DpnII GATC 4 cut(s) 6, 1281, 1363, 1405
DriI GACNNNNNGTC 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 1348
Eam1104I CTCTTC 4 cut(s) 79, 623, 1116, 1269
Eam1105I GACNNNNNGTC 1 cut(s) 214
EarI CTCTTC 4 cut(s) 79, 623, 1116, 1269
Eco130I CCWWGG 4 cut(s) 261, 364, 1351, 1417
Eco24I GRGCYC 1 cut(s) 378
Eco47I GGWCC 2 cut(s) 295, 1267
Eco81I CCTNAGG 1 cut(s) 1191
Eco88I CYCGRG 1 cut(s) 618
EcoO109I RGGNCCY 1 cut(s) 659
EcoRII CCWGG 3 cut(s) 499, 661, 1269
EcoT14I CCWWGG 4 cut(s) 261, 364, 1351, 1417
EcoT22I ATGCAT 1 cut(s) 904
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 4 cut(s) 261, 364, 1351, 1417
FaqI GGGAC 3 cut(s) 286, 1000, 1140
FauI CCCGC 1 cut(s) 210
FbaI TGATCA 1 cut(s) 6
Fnu4HI GCNGC 3 cut(s) 245, 268, 1203
FokI GGATG 5 cut(s) 763, 928, 965, 995, 1211
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 3 cut(s) 245, 268, 1203
GlaI GCGC 1 cut(s) 1387
GluI GCNGC 3 cut(s) 245, 268, 1203
GsuI CTGGAG 1 cut(s) 1410
HaeII RGCGCY 1 cut(s) 1389
HaeIII GGCC 5 cut(s) 267, 661, 1189, 1350, 1416
HapII CCGG 2 cut(s) 293, 453
HgaI GACGC 2 cut(s) 236, 322
HhaI GCGC 1 cut(s) 1388
Hin1I GRCGYC 1 cut(s) 333
Hin6I GCGC 1 cut(s) 1386
HinP1I GCGC 1 cut(s) 1386
HindIII AAGCTT 1 cut(s) 229
HinfI GANTC 3 cut(s) 341, 737, 1021
HpaII CCGG 2 cut(s) 293, 453
HphI GGTGA 6 cut(s) 16, 139, 160, 652, 677, 1159
Hpy188I TCNGA 5 cut(s) 346, 469, 1057, 1262, 1299
Hpy188III TCNNGA 6 cut(s) 283, 545, 927, 1095, 1193, 1403
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 9 cut(s) 142, 181, 196, 343, 472, 628, 670, 985, 1176
HpyCH4III ACNGT 3 cut(s) 157, 274, 1258
HpyCH4IV ACGT 2 cut(s) 114, 239
HpyCH4V TGCA 9 cut(s) 721, 733, 902, 956, 1040, 1081, 1145, 1202, 1229
HpyF10VI GCNNNNNNNGC 2 cut(s) 250, 416
HpyF3I CTNAG 5 cut(s) 324, 377, 1107, 1191, 1287
HpySE526I ACGT 2 cut(s) 114, 239
Hsp92I GRCGYC 1 cut(s) 333
HspAI GCGC 1 cut(s) 1386
Ksp22I TGATCA 1 cut(s) 6
Kzo9I GATC 4 cut(s) 6, 1281, 1363, 1405
LguI GCTCTTC 1 cut(s) 1269
LmnI GCTCC 2 cut(s) 718, 1391
Lsp1109I GCAGC 2 cut(s) 231, 1214
LweI GCATC 2 cut(s) 943, 1189
MaeII ACGT 2 cut(s) 114, 239
MaeIII GTNAC 6 cut(s) 334, 796, 1165, 1237, 1245, 1427
MalI GATC 4 cut(s) 8, 1283, 1365, 1407
MboI GATC 4 cut(s) 6, 1281, 1363, 1405
MhlI GDGCHC 1 cut(s) 378
MlsI TGGCCA 1 cut(s) 1350
MluCI AATT 4 cut(s) 402, 540, 760, 1231
MluNI TGGCCA 1 cut(s) 1350
MmeI TCCRAC 1 cut(s) 982
Mox20I TGGCCA 1 cut(s) 1350
Mph1103I ATGCAT 1 cut(s) 904
MscI TGGCCA 1 cut(s) 1350
MseI TTAA 1 cut(s) 227
MslI CAYNNNNRTG 5 cut(s) 1176, 1338, 1344, 1350, 1356
Msp20I TGGCCA 1 cut(s) 1350
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 2 cut(s) 293, 453
MspR9I CCNGG 5 cut(s) 293, 453, 501, 663, 1271
Mva1269I GAATGC 1 cut(s) 1147
MvaI CCWGG 3 cut(s) 501, 663, 1271
MwoI GCNNNNNNNGC 2 cut(s) 250, 416
NciI CCSGG 2 cut(s) 293, 453
NcoI CCATGG 2 cut(s) 364, 1351
NdeII GATC 4 cut(s) 6, 1281, 1363, 1405
NlaIV GGNNCC 3 cut(s) 597, 660, 714
NmuCI GTSAC 4 cut(s) 334, 796, 1165, 1427
NsiI ATGCAT 1 cut(s) 904
NspI RCATGY 1 cut(s) 1343
NspV TTCGAA 1 cut(s) 758
PaqCI CACCTGC 1 cut(s) 583
PciI ACATGT 1 cut(s) 1339
PciSI GCTCTTC 1 cut(s) 1269
PcsI WCGNNNNNNNCGW 2 cut(s) 473, 1314
PctI GAATGC 1 cut(s) 1147
PfeI GAWTC 3 cut(s) 341, 737, 1021
Pfl23II CGTACG 1 cut(s) 1410
PflMI CCANNNNNTGG 1 cut(s) 370
PfoI TCCNGGA 1 cut(s) 1269
PkrI GCNGC 3 cut(s) 246, 269, 1204
PscI ACATGT 1 cut(s) 1339
PsiI TTATAA 1 cut(s) 356
Psp1406I AACGTT 1 cut(s) 114
Psp6I CCWGG 3 cut(s) 499, 661, 1269
PspGI CCWGG 3 cut(s) 499, 661, 1269
PspLI CGTACG 1 cut(s) 1410
PspN4I GGNNCC 3 cut(s) 597, 660, 714
PspPI GGNCC 4 cut(s) 265, 295, 659, 1267
PvuII CAGCTG 1 cut(s) 323
RsaI GTAC 1 cut(s) 1412
RsaNI GTAC 1 cut(s) 1411
RseI CAYNNNNRTG 5 cut(s) 1176, 1338, 1344, 1350, 1356
SapI GCTCTTC 1 cut(s) 1269
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 3 cut(s) 245, 268, 1203
Sau3AI GATC 4 cut(s) 6, 1281, 1363, 1405
Sau96I GGNCC 4 cut(s) 265, 295, 659, 1267
ScrFI CCNGG 5 cut(s) 293, 453, 501, 663, 1271
SduI GDGCHC 1 cut(s) 378
SfaNI GCATC 2 cut(s) 943, 1189
SfuI TTCGAA 1 cut(s) 758
SinI GGWCC 2 cut(s) 295, 1267
SmiMI CAYNNNNRTG 5 cut(s) 1176, 1338, 1344, 1350, 1356
Sse9I AATT 4 cut(s) 402, 540, 760, 1231
SsiI CCGC 3 cut(s) 203, 268, 944
StyD4I CCNGG 5 cut(s) 291, 451, 499, 661, 1269
StyI CCWWGG 4 cut(s) 261, 364, 1351, 1417
TaaI ACNGT 3 cut(s) 157, 274, 1258
TaiI ACGT 2 cut(s) 117, 242
TaqI TCGA 5 cut(s) 476, 758, 818, 926, 1308
TasI AATT 4 cut(s) 402, 540, 760, 1231
TauI GCSGC 1 cut(s) 270
TfiI GAWTC 3 cut(s) 341, 737, 1021
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TseFI GTSAC 4 cut(s) 334, 796, 1165, 1427
TseI GCWGC 2 cut(s) 244, 1202
Tsp45I GTSAC 4 cut(s) 334, 796, 1165, 1427
TspDTI ATGAA 8 cut(s) 169, 419, 641, 1224, 1326, 1334, 1370, 1374
TspGWI ACGGA 3 cut(s) 353, 1350, 1398
Van91I CCANNNNNTGG 1 cut(s) 370
VpaK11BI GGWCC 2 cut(s) 295, 1267
XapI RAATTY 2 cut(s) 540, 1231
XceI RCATGY 1 cut(s) 1343
XcmI CCANNNNNNNNNTGG 1 cut(s) 132
Zsp2I ATGCAT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.