Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0011194

Physical Location & Seq

Genomic Coordinates Reverse (-)

45777987 .. 45781290

Gene Structure 3304 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00424640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGGTGATCATCACAGAGGAAGAAGACAACCAAAAACGAGACCCCAAACCCTCACCAAGCACCAAACCAACCCACCGTTCAAAACAATCAAAACCCACTCCTCCCCCGTTCGCTTTCTGGTTCTACTTCACAATCACAGTCTCCATCATAACCTTCTTCTTCATCTCCTTCTCTTCTCTCTCTCCGCAAGACCCAAAGTCTTGGTTTTTAAGTTTACCCACATCGCTGCGTCAATACTACTCCAAGGGTCGCACTATAAAGGTCCAGACCCACCCGGACCAATCTGCCTTCGAAGTTTTCACAGCTGAGAAAGGCGTTACAGAATCGGAGAAGGTTATAATTGTTCATGGTTTGGGTCTGAGTTCGTATTCTTTTCGCAAAGTAATCGAAGCCCTGGGTTCCAGAGGGGTTCATGTGGTGGCGTTTGATTTGCCCGGAAATGGGTTTTCGGATAAATCGACGGTGGAGATTGGGGAGAACCCAGGTGGGCTTCTTGGGAGGTTTTCGTTTGTTGTGAGTGAAATTCAAGAAAAGGGTTTGTTTTGGGCATTTGATGAGATTGTGGCAACTGGGCAGGTGCCCTTTGAGGAGATAGAGGCTAGGGCATCGAAGCGAAAAGTAGTGAAGCCGATTGAGTTGGGCCCTGAGGAGGTTGGGAAGGTGTTGGGGCAAGTGATTGAGACAATGGGCTTGGCTCCTGTGCATTTGGTTTTGCATGACTCGGCTTTGGGGATGGTTTCGAATTGGGTTTTGGAGAATGTGCAGTCTGTGAGGAGTGTGACGCTGATTGATACTGGGGCTAAGTCAGCAGGGGCTTTGCCTTTGTGGGTTTTGGAAGTGCCATTGGTTAGGGAGTTGGTGTTGGGGTTTTCGTATGCTCATGCATTGTTGATTAAGATGAGTTGCTCGAGCGGGGTTGATAGTGTGGATTTGAATGCGCATAGAGTTCTTTTGAAGGGGAGGGATGGGACGAAGGCGATTGTTGGAATGGGGAAGAAGTTGAATCGTAGCTTTGATATTGCAGAATGGGGTGGTTCGGATGGACTGAAAGGAGTGCCAATGCAATTGCTTTGGTCTGAACGTTGGTCTAAGGAATGGAGTGAAGAGGGGCGCCAAATTGCGAATGCACTTCCAAAGGCGAACTTTGTCTCCCATTCTGGTGGACGGTGGCCTCAGGAGGATGCAGCCGATGAAGTAGCTGGACGCATTGCTAATTTTGTAACCTCATTACCTGCTACTGTCAGAAAGGTCGCTGAAGAGCCGATCCCTGAGCATATTCAGACGTTGCTTGATGAAGCTGAACAGACTGGTCATGGGCATCTTCATGGTCATGCTGGTCATGAAGAGCATAGTCATGGACATGTTCATGGTGCTGGAGCTGGTTATCCTGATCCGTACGGCCTTGGTCATGGCCACGGATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005783 GO:0006810 GO:0008150 GO:0009605 GO:0009612 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0009914 GO:0009926 GO:0010033 GO:0010817 GO:0012505 GO:0016020 GO:0031090 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0060918 GO:0065007 GO:0065008 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6

Protein Analysis

474

Amino Acids

51.85

Weight (kDa)

6.17

Isoelectric Point (pI)

42.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_1	PF00561	112 - 265	1.2e-12	alpha/beta hydrolase fold
Abhydrolase_6	PF12697	112 - 400	1.6e-08	Alpha/beta hydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54990
fragaria_vesca	FvH4_6g06010
malus_domestica	MD04G1204900.v1.1 MD12G1218600.v1.1
prunus_persica	Prupe.6G324900_v2.0.a1
pyrus_communis	pycom04g18110 pycom12g20410
rosa_chinensis	RchiOBHm_Chr3g0454971
rosa_laevigata	RLG00000025397
rosa_multiflora	Rmu_sc0002169.1_g000009
rosa_roxburghii	Rroxscaffold_6G00424640
rosa_rugosa	Rorug03G0003400 Rorug03G0003500 Rorug03G0003500 Rorug03G0003600
rosa_samantha	Rh3AG063200 Rh3BG065200 Rh3CG064000 Rh3DG065200
rosa_wichuraiana	Rw3G004960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	338
AarI	CACCTGC	1 cut(s)	565
Acc16I	TGCGCA	1 cut(s)	939
Acc36I	ACCTGC	2 cut(s)	565, 1240
AccB1I	GGYRCC	2 cut(s)	577, 1110
AccBSI	CCGCTC	1 cut(s)	912
AciI	CCGC	2 cut(s)	185, 912
AclI	AACGTT	1 cut(s)	1081
AclWI	GGATC	2 cut(s)	1258, 1385
AcoI	YGGCCR	1 cut(s)	1411
AcsI	RAATTY	1 cut(s)	522
AcuI	CTGAAG	1 cut(s)	1275
AcyI	GRCGYC	1 cut(s)	1111
AfaI	GTAC	1 cut(s)	1397
AfiI	CCNNNNNNNGG	3 cut(s)	440, 441, 649
AflIII	ACRYGT	1 cut(s)	1360
AgsI	TTSAA	5 cut(s)	81, 527, 934, 955, 1003
AhdI	GACNNNNNGTC	1 cut(s)	196
AjnI	CCWGG	2 cut(s)	393, 481
AjuI	GAANNNNNNNTTGG	4 cut(s)	61, 93, 734, 766
AloI	GAACNNNNNNTCC	2 cut(s)	1133, 1165
AluBI	AGCT	5 cut(s)	305, 1011, 1199, 1298, 1379
AluI	AGCT	5 cut(s)	305, 1011, 1199, 1298, 1379
Alw26I	GTCTC	4 cut(s)	33, 145, 674, 1153
AlwI	GGATC	2 cut(s)	1258, 1385
Ama87I	CYCGRG	1 cut(s)	907
AoxI	GGCC	4 cut(s)	640, 1169, 1399, 1411
ApaI	GGGCCC	1 cut(s)	644
ApeKI	GCWGC	2 cut(s)	226, 1184
ApoI	RAATTY	1 cut(s)	522
AspLEI	GCGC	2 cut(s)	940, 1113
AspS9I	GGNCC	4 cut(s)	262, 277, 640, 641
AsuC2I	CCSGG	2 cut(s)	275, 435
AsuHPI	GGTGA	2 cut(s)	16, 45
AsuII	TTCGAA	2 cut(s)	291, 740
AvaI	CYCGRG	1 cut(s)	907
AvaII	GGWCC	2 cut(s)	262, 277
AxyI	CCTNAGG	2 cut(s)	645, 1173
BaeGI	GKGCMC	2 cut(s)	582, 644
BalI	TGGCCA	1 cut(s)	1413
BanI	GGYRCC	2 cut(s)	577, 1110
BanII	GRGCYC	1 cut(s)	644
BbsI	GAAGAC	1 cut(s)	30
BbvI	GCAGC	2 cut(s)	213, 1196
BccI	CCATC	5 cut(s)	152, 727, 959, 1034, 1413
BceAI	ACGGC	1 cut(s)	1414
BcgI	CGANNNNNNTGC	2 cut(s)	367, 401
BciT130I	CCWGG	2 cut(s)	395, 483
BclI	TGATCA	1 cut(s)	6
BcnI	CCSGG	2 cut(s)	275, 435
BcoDI	GTCTC	4 cut(s)	33, 145, 674, 1153
BfaI	CTAG	1 cut(s)	600
BfoI	RGCGCY	1 cut(s)	1114
BfuAI	ACCTGC	2 cut(s)	565, 1240
BisI	GCNGC	2 cut(s)	227, 1185
BlsI	GCNGC	2 cut(s)	228, 1186
Bme1390I	CCNGG	4 cut(s)	275, 395, 435, 483
Bme18I	GGWCC	2 cut(s)	262, 277
BmeRI	GACNNNNNGTC	1 cut(s)	196
BmeT110I	CYCGRG	1 cut(s)	907
BmgT120I	GGNCC	4 cut(s)	262, 277, 640, 641
BmiI	GGNNCC	5 cut(s)	400, 579, 642, 696, 1112
BmrFI	CCNGG	4 cut(s)	275, 395, 435, 483
BmrI	ACTGGG	2 cut(s)	579, 804
BmsI	GCATC	3 cut(s)	614, 1171, 1327
BmuI	ACTGGG	2 cut(s)	579, 804
BpiI	GAAGAC	1 cut(s)	30
BpmI	CTGGAG	1 cut(s)	1395
Bpu10I	CCTNAGC	1 cut(s)	1269
Bpu14I	TTCGAA	2 cut(s)	291, 740
BpuMI	CCSGG	2 cut(s)	275, 435
BsaHI	GRCGYC	1 cut(s)	1111
BsaI	GGTCTC	1 cut(s)	33
BsaJI	CCNNGG	6 cut(s)	243, 393, 394, 481, 1402, 1414
BsaXI	ACNNNNNCTCC	4 cut(s)	845, 875, 1133, 1163
Bsc4I	CCNNNNNNNGG	3 cut(s)	440, 441, 649
Bse1I	ACTGG	3 cut(s)	574, 799, 1312
Bse21I	CCTNAGG	2 cut(s)	645, 1173
Bse3DI	GCAATG	1 cut(s)	1206
BseBI	CCWGG	2 cut(s)	395, 483
BseDI	CCNNGG	6 cut(s)	243, 393, 394, 481, 1402, 1414
BseGI	GGATG	5 cut(s)	738, 970, 1045, 1186, 1424
BseLI	CCNNNNNNNGG	3 cut(s)	440, 441, 649
BseMI	GCAATG	1 cut(s)	1206
BseMII	CTCAG	5 cut(s)	297, 350, 636, 1187, 1260
BseNI	ACTGG	3 cut(s)	574, 799, 1312
BseRI	GAGGAG	4 cut(s)	90, 602, 662, 787
BseSI	GKGCMC	2 cut(s)	582, 644
BseXI	GCAGC	2 cut(s)	213, 1196
BsgI	GTGCAG	1 cut(s)	782
BshFI	GGCC	4 cut(s)	642, 1171, 1401, 1413
BshNI	GGYRCC	2 cut(s)	577, 1110
BsiHKCI	CYCGRG	1 cut(s)	907
BsiSI	CCGG	2 cut(s)	275, 435
BsiWI	CGTACG	1 cut(s)	1395
BslFI	GGGAC	1 cut(s)	982
BslI	CCNNNNNNNGG	3 cut(s)	440, 441, 649
BsmAI	GTCTC	4 cut(s)	33, 145, 674, 1153
BsmFI	GGGAC	1 cut(s)	982
BsmI	GAATGC	2 cut(s)	940, 1129
BsnI	GGCC	4 cut(s)	642, 1171, 1401, 1413
Bso31I	GGTCTC	1 cut(s)	33
BsoBI	CYCGRG	1 cut(s)	907
Bsp119I	TTCGAA	2 cut(s)	291, 740
Bsp120I	GGGCCC	1 cut(s)	640
Bsp1286I	GDGCHC	2 cut(s)	582, 644
Bsp143I	GATC	3 cut(s)	6, 1263, 1390
BspACI	CCGC	2 cut(s)	185, 912
BspANI	GGCC	4 cut(s)	642, 1171, 1401, 1413
BspCNI	CTCAG	5 cut(s)	298, 351, 637, 1186, 1261
BspHI	TCATGA	1 cut(s)	1339
BspLI	GGNNCC	5 cut(s)	400, 579, 642, 696, 1112
BspMI	ACCTGC	2 cut(s)	565, 1240
BspPI	GGATC	2 cut(s)	1258, 1385
BspQI	GCTCTTC	2 cut(s)	1251, 1338
BspT104I	TTCGAA	2 cut(s)	291, 740
BspT107I	GGYRCC	2 cut(s)	577, 1110
BspTNI	GGTCTC	1 cut(s)	33
BsrBI	CCGCTC	1 cut(s)	912
BsrDI	GCAATG	1 cut(s)	1206
BsrI	ACTGG	3 cut(s)	574, 799, 1312
BssECI	CCNNGG	6 cut(s)	243, 393, 394, 481, 1402, 1414
BssMI	GATC	3 cut(s)	6, 1263, 1390
BssNI	GRCGYC	1 cut(s)	1111
BssT1I	CCWWGG	2 cut(s)	243, 1402
Bst2UI	CCWGG	2 cut(s)	395, 483
Bst4CI	ACNGT	5 cut(s)	77, 139, 463, 1167, 1240
Bst6I	CTCTTC	4 cut(s)	178, 1098, 1251, 1338
BstACI	GRCGYC	1 cut(s)	1111
BstBI	TTCGAA	2 cut(s)	291, 740
BstDEI	CTNAG	7 cut(s)	306, 359, 645, 801, 1089, 1173, 1269
BstDSI	CCRYGG	1 cut(s)	1414
BstF5I	GGATG	5 cut(s)	738, 970, 1045, 1186, 1424
BstH2I	RGCGCY	1 cut(s)	1114
BstHHI	GCGC	2 cut(s)	940, 1113
BstKTI	GATC	3 cut(s)	9, 1266, 1393
BstMAI	GTCTC	4 cut(s)	33, 145, 674, 1153
BstMBI	GATC	3 cut(s)	6, 1263, 1390
BstMWI	GCNNNNNNNGC	1 cut(s)	806
BstNI	CCWGG	2 cut(s)	395, 483
BstNSI	RCATGY	1 cut(s)	1364
BstSCI	CCNGG	4 cut(s)	273, 393, 433, 481
BstSLI	GKGCMC	2 cut(s)	582, 644
BstV1I	GCAGC	2 cut(s)	213, 1196
BstV2I	GAAGAC	1 cut(s)	30
BstXI	CCANNNNNNTGG	2 cut(s)	201, 1160
Bsu36I	CCTNAGG	2 cut(s)	645, 1173
BsuRI	GGCC	4 cut(s)	642, 1171, 1401, 1413
BtgI	CCRYGG	1 cut(s)	1414
BtgZI	GCGATG	1 cut(s)	207
BtsCI	GGATG	5 cut(s)	738, 970, 1045, 1186, 1424
BveI	ACCTGC	2 cut(s)	565, 1240
CciI	TCATGA	1 cut(s)	1339
CfoI	GCGC	2 cut(s)	940, 1113
Cfr13I	GGNCC	4 cut(s)	262, 277, 640, 641
CseI	GACGC	3 cut(s)	218, 790, 1212
Csp6I	GTAC	1 cut(s)	1396
CviQI	GTAC	1 cut(s)	1396
DdeI	CTNAG	7 cut(s)	306, 359, 645, 801, 1089, 1173, 1269
DinI	GGCGCC	1 cut(s)	1112
DpnI	GATC	3 cut(s)	8, 1265, 1392
DpnII	GATC	3 cut(s)	6, 1263, 1390
DriI	GACNNNNNGTC	1 cut(s)	196
EaeI	YGGCCR	1 cut(s)	1411
Eam1104I	CTCTTC	4 cut(s)	178, 1098, 1251, 1338
Eam1105I	GACNNNNNGTC	1 cut(s)	196
EarI	CTCTTC	4 cut(s)	178, 1098, 1251, 1338
Eco130I	CCWWGG	2 cut(s)	243, 1402
Eco24I	GRGCYC	1 cut(s)	644
Eco31I	GGTCTC	1 cut(s)	33
Eco47I	GGWCC	2 cut(s)	262, 277
Eco57I	CTGAAG	1 cut(s)	1275
Eco81I	CCTNAGG	2 cut(s)	645, 1173
Eco88I	CYCGRG	1 cut(s)	907
EcoO109I	RGGNCCY	1 cut(s)	641
EcoRII	CCWGG	2 cut(s)	393, 481
EcoT14I	CCWWGG	2 cut(s)	243, 1402
EcoT22I	ATGCAT	1 cut(s)	886
EcoT38I	GRGCYC	1 cut(s)	644
EgeI	GGCGCC	1 cut(s)	1112
EheI	GGCGCC	1 cut(s)	1112
ErhI	CCWWGG	2 cut(s)	243, 1402
FalI	AAGNNNNNCTT	2 cut(s)	1127, 1159
FaqI	GGGAC	1 cut(s)	982
FauI	CCCGC	1 cut(s)	905
FbaI	TGATCA	1 cut(s)	6
Fnu4HI	GCNGC	2 cut(s)	227, 1185
FokI	GGATG	4 cut(s)	745, 977, 1052, 1193
FriOI	GRGCYC	1 cut(s)	644
Fsp4HI	GCNGC	2 cut(s)	227, 1185
FspAI	RTGCGCAY	1 cut(s)	939
FspBI	CTAG	1 cut(s)	600
FspI	TGCGCA	1 cut(s)	939
GlaI	GCGC	2 cut(s)	939, 1112
GluI	GCNGC	2 cut(s)	227, 1185
GsuI	CTGGAG	1 cut(s)	1395
HaeII	RGCGCY	1 cut(s)	1114
HaeIII	GGCC	4 cut(s)	642, 1171, 1401, 1413
HapII	CCGG	2 cut(s)	275, 435
HgaI	GACGC	3 cut(s)	218, 790, 1212
HhaI	GCGC	2 cut(s)	940, 1113
Hin1I	GRCGYC	1 cut(s)	1111
Hin6I	GCGC	2 cut(s)	938, 1111
HinP1I	GCGC	2 cut(s)	938, 1111
HinfI	GANTC	3 cut(s)	323, 719, 1003
HpaII	CCGG	2 cut(s)	275, 435
HphI	GGTGA	2 cut(s)	16, 45
Hpy166II	GTNNAC	2 cut(s)	215, 1163
Hpy188I	TCNGA	7 cut(s)	328, 360, 451, 1039, 1078, 1244, 1281
Hpy188III	TCNNGA	6 cut(s)	265, 402, 527, 1175, 1340, 1388
Hpy8I	GTNNAC	2 cut(s)	215, 1163
Hpy99I	CGWCG	1 cut(s)	463
HpyAV	CCTTC	7 cut(s)	163, 178, 298, 325, 652, 949, 967
HpyCH4III	ACNGT	5 cut(s)	77, 139, 463, 1167, 1240
HpyCH4IV	ACGT	2 cut(s)	1081, 1283
HpyCH4V	TGCA	8 cut(s)	703, 715, 763, 884, 1022, 1063, 1127, 1184
HpyF10VI	GCNNNNNNNGC	1 cut(s)	806
HpyF3I	CTNAG	7 cut(s)	306, 359, 645, 801, 1089, 1173, 1269
HpySE526I	ACGT	2 cut(s)	1081, 1283
Hsp92I	GRCGYC	1 cut(s)	1111
HspAI	GCGC	2 cut(s)	938, 1111
KasI	GGCGCC	1 cut(s)	1110
Ksp22I	TGATCA	1 cut(s)	6
Kzo9I	GATC	3 cut(s)	6, 1263, 1390
LguI	GCTCTTC	2 cut(s)	1251, 1338
LmnI	GCTCC	2 cut(s)	700, 1376
Lsp1109I	GCAGC	2 cut(s)	213, 1196
LweI	GCATC	3 cut(s)	614, 1171, 1327
MaeI	CTAG	1 cut(s)	600
MaeII	ACGT	2 cut(s)	1081, 1283
MaeIII	GTNAC	3 cut(s)	316, 778, 1219
MalI	GATC	3 cut(s)	8, 1265, 1392
MbiI	CCGCTC	1 cut(s)	912
MboI	GATC	3 cut(s)	6, 1263, 1390
MfeI	CAATTG	1 cut(s)	1064
MhlI	GDGCHC	2 cut(s)	582, 644
MlsI	TGGCCA	1 cut(s)	1413
MluCI	AATT	6 cut(s)	339, 522, 742, 1064, 1116, 1213
MluNI	TGGCCA	1 cut(s)	1413
Mly113I	GGCGCC	1 cut(s)	1111
MlyI	GAGTC	1 cut(s)	713
MmeI	TCCRAC	1 cut(s)	964
Mox20I	TGGCCA	1 cut(s)	1413
Mph1103I	ATGCAT	1 cut(s)	886
MscI	TGGCCA	1 cut(s)	1413
MseI	TTAA	2 cut(s)	209, 894
MslI	CAYNNNNRTG	6 cut(s)	1158, 1323, 1329, 1353, 1359, 1365
Msp20I	TGGCCA	1 cut(s)	1413
MspA1I	CMGCKG	1 cut(s)	305
MspI	CCGG	2 cut(s)	275, 435
MspR9I	CCNGG	4 cut(s)	275, 395, 435, 483
MunI	CAATTG	1 cut(s)	1064
Mva1269I	GAATGC	2 cut(s)	940, 1129
MvaI	CCWGG	2 cut(s)	395, 483
MwoI	GCNNNNNNNGC	1 cut(s)	806
NarI	GGCGCC	1 cut(s)	1111
NciI	CCSGG	2 cut(s)	275, 435
NdeII	GATC	3 cut(s)	6, 1263, 1390
NlaIV	GGNNCC	5 cut(s)	400, 579, 642, 696, 1112
NmeAIII	GCCGAG	1 cut(s)	701
NmuCI	GTSAC	1 cut(s)	778
NsbI	TGCGCA	1 cut(s)	939
NsiI	ATGCAT	1 cut(s)	886
NspI	RCATGY	1 cut(s)	1364
NspV	TTCGAA	2 cut(s)	291, 740
PaeR7I	CTCGAG	1 cut(s)	907
PagI	TCATGA	1 cut(s)	1339
PaqCI	CACCTGC	1 cut(s)	565
PasI	CCCWGGG	1 cut(s)	394
PciI	ACATGT	1 cut(s)	1360
PciSI	GCTCTTC	2 cut(s)	1251, 1338
PcsI	WCGNNNNNNNCGW	1 cut(s)	455
PctI	GAATGC	2 cut(s)	940, 1129
PfeI	GAWTC	2 cut(s)	323, 1003
Pfl23II	CGTACG	1 cut(s)	1395
PkrI	GCNGC	2 cut(s)	228, 1186
PleI	GAGTC	1 cut(s)	713
PluTI	GGCGCC	1 cut(s)	1114
PpsI	GAGTC	1 cut(s)	713
PscI	ACATGT	1 cut(s)	1360
PsiI	TTATAA	1 cut(s)	338
Psp1406I	AACGTT	1 cut(s)	1081
Psp6I	CCWGG	2 cut(s)	393, 481
PspGI	CCWGG	2 cut(s)	393, 481
PspLI	CGTACG	1 cut(s)	1395
PspN4I	GGNNCC	5 cut(s)	400, 579, 642, 696, 1112
PspOMI	GGGCCC	1 cut(s)	640
PspPI	GGNCC	4 cut(s)	262, 277, 640, 641
PspXI	VCTCGAGB	1 cut(s)	907
PvuII	CAGCTG	1 cut(s)	305
RsaI	GTAC	1 cut(s)	1397
RsaNI	GTAC	1 cut(s)	1396
RseI	CAYNNNNRTG	6 cut(s)	1158, 1323, 1329, 1353, 1359, 1365
SapI	GCTCTTC	2 cut(s)	1251, 1338
SaqAI	TTAA	2 cut(s)	209, 894
SatI	GCNGC	2 cut(s)	227, 1185
Sau3AI	GATC	3 cut(s)	6, 1263, 1390
Sau96I	GGNCC	4 cut(s)	262, 277, 640, 641
SchI	GAGTC	1 cut(s)	713
ScrFI	CCNGG	4 cut(s)	275, 395, 435, 483
SduI	GDGCHC	2 cut(s)	582, 644
SfaNI	GCATC	3 cut(s)	614, 1171, 1327
SfoI	GGCGCC	1 cut(s)	1112
Sfr274I	CTCGAG	1 cut(s)	907
SfuI	TTCGAA	2 cut(s)	291, 740
SinI	GGWCC	2 cut(s)	262, 277
SlaI	CTCGAG	1 cut(s)	907
SmiMI	CAYNNNNRTG	6 cut(s)	1158, 1323, 1329, 1353, 1359, 1365
SmlI	CTYRAG	1 cut(s)	907
SmoI	CTYRAG	1 cut(s)	907
Sse9I	AATT	6 cut(s)	339, 522, 742, 1064, 1116, 1213
SsiI	CCGC	2 cut(s)	185, 912
SspDI	GGCGCC	1 cut(s)	1110
SspMI	CTAG	1 cut(s)	600
StyD4I	CCNGG	4 cut(s)	273, 393, 433, 481
StyI	CCWWGG	2 cut(s)	243, 1402
TaaI	ACNGT	5 cut(s)	77, 139, 463, 1167, 1240
TaiI	ACGT	2 cut(s)	1084, 1286
TaqI	TCGA	6 cut(s)	291, 387, 458, 608, 740, 908
TasI	AATT	6 cut(s)	339, 522, 742, 1064, 1116, 1213
TfiI	GAWTC	2 cut(s)	323, 1003
Tru1I	TTAA	2 cut(s)	209, 894
Tru9I	TTAA	2 cut(s)	209, 894
TseFI	GTSAC	1 cut(s)	778
TseI	GCWGC	2 cut(s)	226, 1184
Tsp45I	GTSAC	1 cut(s)	778
TspDTI	ATGAA	8 cut(s)	151, 335, 401, 1206, 1308, 1313, 1355, 1356
TspGWI	ACGGA	1 cut(s)	1383
VpaK11BI	GGWCC	2 cut(s)	262, 277
XapI	RAATTY	1 cut(s)	522
XceI	RCATGY	1 cut(s)	1364
XhoI	CTCGAG	1 cut(s)	907
XspI	CTAG	1 cut(s)	600
Zsp2I	ATGCAT	1 cut(s)	886

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.