FvH4_6g06550

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010248
Physical Location & Seq
Forward (+)
3839794 .. 3843745
3952 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g06550.t1

Sequence Viewer

Length: 963 bp
ATGGCGAGCAGTTTGCAGATACCAATCTGCATACCCACCTGTTCTTCCTCTCGAAAGTCACAAACTTTCTCAACCTCTTGCTCCCTCAACGCCAGAGCCAAAGCCAAAATCCCAATCCCTCGTATAAACCCTAGAGACCCCTTCCTCTCCCGCCTCGCCTCCGTCGCCGTCAACTCGCCGGAAACATTGCTCAACCGCCCCTCCAACAACTCCGACTCTCTTCCTTACTTGGATATCTTCGACGAGCCCAAGCTCATGGCCACCCCTGCCCAAGTGGAAAGATCAGTTTCTTACAATGAGCATAGACCAAAGAAGCCTCCTCCGGATTTGCCCTCATTGCTACTCCACGGCAGAATAGTTTATATCGGCATGCCTCTCGTACCAGCAGTCACAGAGCTTATTGTTGCAGAGCTGATGTATCTGCAATGGATGGATCCGAAACAACCGATCTATATTTATATTAATTCAACTGGAACAACACGTGATGATGGTGAAACGGTTGGCATGGAGTCGGAGGGTTTTGCCATCTATGATGCGATGATGCAGTTGAAAAATGAGATACATACTGTTGCTGTTGGGGCTGCTATAGGTCAAGCTTGTTTATTGCTTGCAGCAGGAACTAAGGGAAACCGGTTCATGATGCCACATGCCAAAGCCATGATCCAGCAGCCTCGTGTCCCATCATCTGGTCTGATGCCTGCGAGCGATGTTCTTATTCGTGCGAAAGAGGTAATTACAAATAGGGACACTCTTGTAAAACTTCTGTCCAAGCACACAGGAAATTCCGTGGAGGCTGTATCTAACCTGATGAAAAGACCATATTATATGGATTCTACAACGGCTAAAGAATTCGGTGTTATTGACAAAGTCTTGTGGCGTGGTCAAGAAAAGATTATGGCAGATGTAGCCTCCCCTGAGGATTGGGACAAGAATGCTGGTGTCAAAGTTGTAGATGAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLP_protease

Protein Analysis

321

Amino Acids

35.22

Weight (kDa)

8.25

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 112 - 291 9.3e-44 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 686
AccIII TCCGGA 1 cut(s) 322
AciI CCGC 2 cut(s) 151, 196
AclWI GGATC 3 cut(s) 428, 441, 655
AcoI YGGCCR 1 cut(s) 258
AcsI RAATTY 2 cut(s) 781, 848
AcvI CACGTG 1 cut(s) 482
AfaI GTAC 1 cut(s) 381
AfiI CCNNNNNNNGG 1 cut(s) 686
AflIII ACRYGT 1 cut(s) 479
AgeI ACCGGT 1 cut(s) 630
AgsI TTSAA 2 cut(s) 468, 550
AluBI AGCT 4 cut(s) 253, 397, 412, 596
AluI AGCT 4 cut(s) 253, 397, 412, 596
Alw26I GTCTC 1 cut(s) 129
AlwI GGATC 3 cut(s) 428, 441, 655
Aor13HI TCCGGA 1 cut(s) 322
AoxI GGCC 1 cut(s) 258
ApeKI GCWGC 3 cut(s) 581, 611, 667
ApoI RAATTY 2 cut(s) 781, 848
AseI ATTAAT 1 cut(s) 462
AsiGI ACCGGT 1 cut(s) 630
AsuHPI GGTGA 1 cut(s) 503
AxyI CCTNAGG 1 cut(s) 915
BalI TGGCCA 1 cut(s) 260
BamHI GGATCC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 249
BauI CACGAG 1 cut(s) 672
BbrPI CACGTG 1 cut(s) 482
BbvI GCAGC 3 cut(s) 568, 623, 679
BccI CCATC 4 cut(s) 424, 482, 533, 688
BceAI ACGGC 3 cut(s) 152, 364, 855
BcgI CGANNNNNNTGC 3 cut(s) 29, 358, 392
BcoDI GTCTC 1 cut(s) 129
BfaI CTAG 1 cut(s) 132
BfmI CTRYAG 1 cut(s) 585
BisI GCNGC 3 cut(s) 582, 612, 668
BlsI GCNGC 3 cut(s) 583, 613, 669
BmiI GGNNCC 1 cut(s) 435
BmsI GCATC 4 cut(s) 523, 531, 630, 684
BsaAI YACGTR 1 cut(s) 482
BsaBI GATNNNNATC 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 346, 786
BsaWI WCCGGW 2 cut(s) 322, 630
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 1 cut(s) 686
Bse118I RCCGGY 1 cut(s) 630
Bse1I ACTGG 1 cut(s) 475
Bse21I CCTNAGG 1 cut(s) 915
Bse3DI GCAATG 3 cut(s) 185, 335, 431
Bse8I GATNNNNATC 1 cut(s) 23
BseAI TCCGGA 1 cut(s) 322
BseDI CCNNGG 2 cut(s) 346, 786
BseGI GGATG 1 cut(s) 435
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 1 cut(s) 686
BseMI GCAATG 3 cut(s) 185, 335, 431
BseMII CTCAG 1 cut(s) 906
BseNI ACTGG 1 cut(s) 475
BseRI GAGGAG 1 cut(s) 309
BseXI GCAGC 3 cut(s) 568, 623, 679
BshFI GGCC 1 cut(s) 260
BshTI ACCGGT 1 cut(s) 630
BsiSI CCGG 3 cut(s) 179, 323, 631
BslFI GGGAC 3 cut(s) 662, 758, 938
BslI CCNNNNNNNGG 1 cut(s) 686
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 3 cut(s) 662, 758, 938
BsmI GAATGC 1 cut(s) 937
BsnI GGCC 1 cut(s) 260
Bso31I GGTCTC 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 249
Bsp13I TCCGGA 1 cut(s) 322
Bsp143I GATC 4 cut(s) 281, 433, 447, 660
BspACI CCGC 2 cut(s) 151, 196
BspANI GGCC 1 cut(s) 260
BspCNI CTCAG 1 cut(s) 907
BspEI TCCGGA 1 cut(s) 322
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 1 cut(s) 435
BspPI GGATC 3 cut(s) 428, 441, 655
BspTNI GGTCTC 1 cut(s) 129
BsrDI GCAATG 3 cut(s) 185, 335, 431
BsrFI RCCGGY 1 cut(s) 630
BsrI ACTGG 1 cut(s) 475
BssAI RCCGGY 1 cut(s) 630
BssECI CCNNGG 2 cut(s) 346, 786
BssMI GATC 4 cut(s) 281, 433, 447, 660
BssSI CACGAG 1 cut(s) 672
Bst2BI CACGAG 1 cut(s) 672
Bst4CI ACNGT 2 cut(s) 499, 568
Bst6I CTCTTC 1 cut(s) 225
BstBAI YACGTR 1 cut(s) 482
BstC8I GCNNGC 5 cut(s) 7, 371, 609, 699, 703
BstDEI CTNAG 2 cut(s) 621, 915
BstDSI CCRYGG 2 cut(s) 346, 786
BstF5I GGATG 1 cut(s) 435
BstKTI GATC 4 cut(s) 284, 436, 450, 663
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 4 cut(s) 281, 433, 447, 660
BstMWI GCNNNNNNNGC 5 cut(s) 164, 266, 337, 578, 905
BstNSI RCATGY 2 cut(s) 373, 650
BstSFI CTRYAG 1 cut(s) 585
BstV1I GCAGC 3 cut(s) 568, 623, 679
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 256
BstYI RGATCY 1 cut(s) 433
Bsu36I CCTNAGG 1 cut(s) 915
BsuRI GGCC 1 cut(s) 260
BtgI CCRYGG 2 cut(s) 346, 786
BtgZI GCGATG 2 cut(s) 551, 720
BtsCI GGATG 1 cut(s) 435
Cac8I GCNNGC 5 cut(s) 7, 371, 609, 699, 703
CciI TCATGA 1 cut(s) 636
Cfr10I RCCGGY 1 cut(s) 630
Csp6I GTAC 1 cut(s) 380
CspAI ACCGGT 1 cut(s) 630
CviAII CATG 6 cut(s) 256, 370, 505, 637, 647, 658
CviQI GTAC 1 cut(s) 380
DdeI CTNAG 2 cut(s) 621, 915
DpnI GATC 4 cut(s) 283, 435, 449, 662
DpnII GATC 4 cut(s) 281, 433, 447, 660
EaeI YGGCCR 1 cut(s) 258
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 129
Eco32I GATATC 1 cut(s) 235
Eco72I CACGTG 1 cut(s) 482
Eco81I CCTNAGG 1 cut(s) 915
EcoRI GAATTC 1 cut(s) 848
EcoRV GATATC 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 249
FaeI CATG 6 cut(s) 259, 373, 508, 640, 650, 661
FaqI GGGAC 3 cut(s) 662, 758, 938
FatI CATG 6 cut(s) 255, 369, 504, 636, 646, 657
FauI CCCGC 1 cut(s) 158
Fnu4HI GCNGC 3 cut(s) 582, 612, 668
FokI GGATG 1 cut(s) 442
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 3 cut(s) 582, 612, 668
FspBI CTAG 1 cut(s) 132
GluI GCNGC 3 cut(s) 582, 612, 668
HaeIII GGCC 1 cut(s) 260
HapII CCGG 3 cut(s) 179, 323, 631
Hin1II CATG 6 cut(s) 259, 373, 508, 640, 650, 661
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 594
HinfI GANTC 3 cut(s) 215, 509, 830
HpaII CCGG 3 cut(s) 179, 323, 631
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 4 cut(s) 214, 438, 514, 693
Hpy188III TCNNGA 4 cut(s) 51, 323, 637, 884
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 2 cut(s) 167, 245
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 2 cut(s) 499, 568
HpyCH4IV ACGT 1 cut(s) 481
HpyCH4V TGCA 6 cut(s) 16, 30, 407, 424, 544, 611
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 266, 337, 578, 905
HpyF3I CTNAG 2 cut(s) 621, 915
HpySE526I ACGT 1 cut(s) 481
Hsp92II CATG 6 cut(s) 259, 373, 508, 640, 650, 661
Kpn2I TCCGGA 1 cut(s) 322
Kzo9I GATC 4 cut(s) 281, 433, 447, 660
LmnI GCTCC 1 cut(s) 86
Lsp1109I GCAGC 3 cut(s) 568, 623, 679
LweI GCATC 4 cut(s) 523, 531, 630, 684
MaeI CTAG 1 cut(s) 132
MaeII ACGT 1 cut(s) 481
MaeIII GTNAC 2 cut(s) 57, 388
MalI GATC 4 cut(s) 283, 435, 449, 662
MboI GATC 4 cut(s) 281, 433, 447, 660
MboII GAAGA 3 cut(s) 36, 212, 229
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 1 cut(s) 249
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 4 cut(s) 463, 732, 781, 848
MluNI TGGCCA 1 cut(s) 260
MlyI GAGTC 2 cut(s) 209, 518
MmeI TCCRAC 3 cut(s) 228, 237, 492
Mox20I TGGCCA 1 cut(s) 260
MroI TCCGGA 1 cut(s) 322
MscI TGGCCA 1 cut(s) 260
MseI TTAA 1 cut(s) 462
Msp20I TGGCCA 1 cut(s) 260
MspI CCGG 3 cut(s) 179, 323, 631
Mva1269I GAATGC 1 cut(s) 937
MwoI GCNNNNNNNGC 5 cut(s) 164, 266, 337, 578, 905
NdeII GATC 4 cut(s) 281, 433, 447, 660
NlaIII CATG 6 cut(s) 259, 373, 508, 640, 650, 661
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 2 cut(s) 57, 388
NspI RCATGY 2 cut(s) 373, 650
PaeI GCATGC 1 cut(s) 373
PagI TCATGA 1 cut(s) 636
PctI GAATGC 1 cut(s) 937
PfeI GAWTC 1 cut(s) 830
PflFI GACNNNGTC 1 cut(s) 866
PflMI CCANNNNNTGG 1 cut(s) 686
PinAI ACCGGT 1 cut(s) 630
PkrI GCNGC 3 cut(s) 583, 613, 669
PleI GAGTC 2 cut(s) 209, 517
PmaCI CACGTG 1 cut(s) 482
PmlI CACGTG 1 cut(s) 482
PpsI GAGTC 2 cut(s) 209, 517
Ppu21I YACGTR 1 cut(s) 482
PshBI ATTAAT 1 cut(s) 462
PspCI CACGTG 1 cut(s) 482
PspN4I GGNNCC 1 cut(s) 435
PsuI RGATCY 1 cut(s) 433
PsyI GACNNNGTC 1 cut(s) 866
RsaI GTAC 1 cut(s) 381
RsaNI GTAC 1 cut(s) 380
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 3 cut(s) 582, 612, 668
Sau3AI GATC 4 cut(s) 281, 433, 447, 660
SchI GAGTC 2 cut(s) 209, 518
SduI GDGCHC 1 cut(s) 249
SfaNI GCATC 4 cut(s) 523, 531, 630, 684
SfcI CTRYAG 1 cut(s) 585
SphI GCATGC 1 cut(s) 373
Sse9I AATT 4 cut(s) 463, 732, 781, 848
SsiI CCGC 2 cut(s) 151, 196
SspMI CTAG 1 cut(s) 132
TaaI ACNGT 2 cut(s) 499, 568
TaiI ACGT 1 cut(s) 484
TaqI TCGA 2 cut(s) 52, 240
TasI AATT 4 cut(s) 463, 732, 781, 848
TfiI GAWTC 1 cut(s) 830
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TseFI GTSAC 2 cut(s) 57, 388
TseI GCWGC 3 cut(s) 581, 611, 667
Tsp45I GTSAC 2 cut(s) 57, 388
TspDTI ATGAA 2 cut(s) 625, 824
TspGWI ACGGA 2 cut(s) 151, 775
Tth111I GACNNNGTC 1 cut(s) 866
Van91I CCANNNNNTGG 1 cut(s) 686
VspI ATTAAT 1 cut(s) 462
XapI RAATTY 2 cut(s) 781, 848
XceI RCATGY 2 cut(s) 373, 650
XspI CTAG 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.