MD12G1213400.v1.1

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010248
Physical Location & Seq
Reverse (-)
29181182 .. 29185015
3834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1213400.v1.1.491

Sequence Viewer

Length: 984 bp
ATGGCGAGCAGTTTGCAGCTGCCAATCTCCATATCGTCAGCTTCTACGTCGTCGTTTAGACCCAGAAATGCTCGGAAGTTCACAGCTTTTTGTTCCGTTAACGCGAAAGCAACGGCCAAAATCCCGATTCCTCCGATAAACCCCAAGGACCCCTTTCTTTCGAGGCTCGCTGCGGTGGCTGCCAAGTCCCCGGAAACCTTGCTGAACCGGCCGCCTCAAAATTCCGACTCTCTCCCGTACTTGGATGTGTTTGATGAACCTATTCTCATGGCCACTCCTGCCCAAGTGGAAAGATCGGTTTCTTACAACGAGCATAGGCCCAGGAAGCCACCTCCAGACCTGCCCTCATTGTTACTCCACGGAAGAATAGTTTATATTGGCATGCCTTTAGTGCCAGCAGTAACAGAGCTTATTGTTGCGGAATTGATGTATCTGCAATGGATGGATCCTAAACAACCAATATATATTTATATTAATTCGACGGGAACCACTCGTGATGATGGTGAAACCGTTGGCATGGAGTCAGAGGGATTTGCTATCTATGATGCTATGATGCAGTTGAAAAATGAGATACATACAGTCGCCGTTGGGGCTGCTATAGGTCAAGCTTGTCTATTGCTTTCAGCAGGAACCGAAGGCAAACGATTTATGATGCCACATGCCAAAGCAATGATCCAGCAACCTCGTGTCCCATCATCTGGACTGATGCCTGCTAGTGACGTTCTTATTCGTGCAAAAGAGGTAATCACAAATAGGGACATTCTCGTAAAGCTTCTGGCCAAACACACAGGAAATTCAGAGGAGGCTGTTGCTAAGGTGATGAGAAGACCATTTTATATGGATTCTACAACTGCTAAACAATTCGGGGTGATTGACAAGATCCTTTGGCGTGGTCAAGAAAAGATAATGGCAGATGTAGCCTCCCCTGAGGAATGGGATAAGACAGCTGGCGTCAAAGTTGTAGATGAACTTGGTGGTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLP_protease

Protein Analysis

328

Amino Acids

35.85

Weight (kDa)

8.4

Isoelectric Point (pI)

50.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 116 - 295 1.8e-42 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 348
AccB7I CCANNNNNTGG 1 cut(s) 698
AccII CGCG 1 cut(s) 104
AciI CCGC 3 cut(s) 173, 212, 419
AclWI GGATC 4 cut(s) 440, 453, 667, 874
AcoI YGGCCR 4 cut(s) 114, 209, 270, 777
AcsI RAATTY 2 cut(s) 220, 793
AcyI GRCGYC 1 cut(s) 951
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 2 cut(s) 241, 698
AgsI TTSAA 1 cut(s) 562
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 7 cut(s) 19, 41, 86, 409, 608, 772, 947
AluI AGCT 7 cut(s) 19, 41, 86, 409, 608, 772, 947
AlwI GGATC 4 cut(s) 440, 453, 667, 874
AoxI GGCC 5 cut(s) 114, 209, 270, 317, 777
ApeKI GCWGC 5 cut(s) 16, 19, 170, 179, 593
ApoI RAATTY 2 cut(s) 220, 793
AseI ATTAAT 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 261
AspS9I GGNCC 2 cut(s) 148, 318
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 3 cut(s) 515, 829, 880
AvaII GGWCC 1 cut(s) 148
AxyI CCTNAGG 1 cut(s) 927
BalI TGGCCA 2 cut(s) 272, 779
BamHI GGATCC 1 cut(s) 445
BauI CACGAG 2 cut(s) 492, 684
BbsI GAAGAC 1 cut(s) 832
BbvI GCAGC 5 cut(s) 6, 28, 157, 166, 580
BccI CCATC 3 cut(s) 436, 494, 700
BceAI ACGGC 2 cut(s) 129, 569
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 322
BcnI CCSGG 1 cut(s) 191
BfaI CTAG 1 cut(s) 714
BfmI CTRYAG 1 cut(s) 597
BfuAI ACCTGC 1 cut(s) 348
BglI GCCNNNNNGGC 1 cut(s) 590
BisI GCNGC 6 cut(s) 17, 20, 171, 180, 212, 594
BlsI GCNGC 6 cut(s) 18, 21, 172, 181, 213, 595
Bme1390I CCNGG 2 cut(s) 191, 322
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 148, 318
BmiI GGNNCC 4 cut(s) 150, 447, 487, 631
BmrFI CCNGG 2 cut(s) 191, 322
BmsI GCATC 4 cut(s) 535, 543, 642, 696
BpiI GAAGAC 1 cut(s) 832
BpmI CTGGAG 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 813
BpuMI CCSGG 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 951
BsaJI CCNNGG 4 cut(s) 144, 189, 320, 358
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 698
Bse118I RCCGGY 1 cut(s) 207
Bse21I CCTNAGG 1 cut(s) 927
Bse3DI GCAATG 2 cut(s) 443, 675
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 4 cut(s) 144, 189, 320, 358
BseGI GGATG 2 cut(s) 250, 447
BseLI CCNNNNNNNGG 2 cut(s) 241, 698
BseMI GCAATG 2 cut(s) 443, 675
BseMII CTCAG 1 cut(s) 918
BseRI GAGGAG 1 cut(s) 815
BseX3I CGGCCG 1 cut(s) 209
BseXI GCAGC 5 cut(s) 6, 28, 157, 166, 580
Bsh1236I CGCG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 212
BshFI GGCC 5 cut(s) 116, 211, 272, 319, 779
BsiEI CGRYCG 1 cut(s) 212
BsiSI CCGG 2 cut(s) 191, 208
BslFI GGGAC 3 cut(s) 172, 674, 770
BslI CCNNNNNNNGG 2 cut(s) 241, 698
BsmFI GGGAC 3 cut(s) 172, 674, 770
BsnI GGCC 5 cut(s) 116, 211, 272, 319, 779
Bsp143I GATC 4 cut(s) 293, 445, 672, 879
BspACI CCGC 3 cut(s) 173, 212, 419
BspANI GGCC 5 cut(s) 116, 211, 272, 319, 779
BspCNI CTCAG 1 cut(s) 919
BspFNI CGCG 1 cut(s) 104
BspLI GGNNCC 4 cut(s) 150, 447, 487, 631
BspMI ACCTGC 1 cut(s) 348
BspPI GGATC 4 cut(s) 440, 453, 667, 874
BsrDI GCAATG 2 cut(s) 443, 675
BsrFI RCCGGY 1 cut(s) 207
BssAI RCCGGY 1 cut(s) 207
BssECI CCNNGG 4 cut(s) 144, 189, 320, 358
BssMI GATC 4 cut(s) 293, 445, 672, 879
BssNI GRCGYC 1 cut(s) 951
BssSI CACGAG 2 cut(s) 492, 684
BssT1I CCWWGG 1 cut(s) 144
Bst2BI CACGAG 2 cut(s) 492, 684
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 2 cut(s) 511, 580
BstACI GRCGYC 1 cut(s) 951
BstC8I GCNNGC 6 cut(s) 7, 168, 383, 396, 711, 949
BstDEI CTNAG 2 cut(s) 813, 927
BstDSI CCRYGG 1 cut(s) 358
BstF5I GGATG 2 cut(s) 250, 447
BstFNI CGCG 1 cut(s) 104
BstKTI GATC 4 cut(s) 296, 448, 675, 882
BstMBI GATC 4 cut(s) 293, 445, 672, 879
BstMCI CGRYCG 1 cut(s) 212
BstMWI GCNNNNNNNGC 8 cut(s) 176, 179, 208, 278, 325, 391, 590, 917
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 2 cut(s) 385, 662
BstSCI CCNGG 2 cut(s) 189, 320
BstSFI CTRYAG 1 cut(s) 597
BstUI CGCG 1 cut(s) 104
BstV1I GCAGC 5 cut(s) 6, 28, 157, 166, 580
BstV2I GAAGAC 1 cut(s) 832
BstX2I RGATCY 2 cut(s) 445, 879
BstYI RGATCY 2 cut(s) 445, 879
BstZI CGGCCG 1 cut(s) 209
Bsu36I CCTNAGG 1 cut(s) 927
BsuRI GGCC 5 cut(s) 116, 211, 272, 319, 779
BtgI CCRYGG 1 cut(s) 358
BtsCI GGATG 2 cut(s) 250, 447
BveI ACCTGC 1 cut(s) 348
Cac8I GCNNGC 6 cut(s) 7, 168, 383, 396, 711, 949
Cfr10I RCCGGY 1 cut(s) 207
Cfr13I GGNCC 2 cut(s) 148, 318
CseI GACGC 1 cut(s) 940
Csp6I GTAC 1 cut(s) 238
CviAII CATG 4 cut(s) 268, 382, 517, 659
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 2 cut(s) 813, 927
DpnI GATC 4 cut(s) 295, 447, 674, 881
DpnII GATC 4 cut(s) 293, 445, 672, 879
EaeI YGGCCR 4 cut(s) 114, 209, 270, 777
EagI CGGCCG 1 cut(s) 209
EclXI CGGCCG 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 148
Eco52I CGGCCG 1 cut(s) 209
Eco81I CCTNAGG 1 cut(s) 927
EcoO109I RGGNCCY 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 320
EcoT14I CCWWGG 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 4 cut(s) 271, 385, 520, 662
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 3 cut(s) 172, 674, 770
FatI CATG 4 cut(s) 267, 381, 516, 658
Fnu4HI GCNGC 6 cut(s) 17, 20, 171, 180, 212, 594
FokI GGATG 2 cut(s) 257, 454
Fsp4HI GCNGC 6 cut(s) 17, 20, 171, 180, 212, 594
FspBI CTAG 1 cut(s) 714
GluI GCNGC 6 cut(s) 17, 20, 171, 180, 212, 594
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 5 cut(s) 116, 211, 272, 319, 779
HapII CCGG 2 cut(s) 191, 208
HgaI GACGC 1 cut(s) 940
Hin1I GRCGYC 1 cut(s) 951
Hin1II CATG 4 cut(s) 271, 385, 520, 662
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 2 cut(s) 606, 770
HinfI GANTC 4 cut(s) 127, 227, 521, 842
HpaI GTTAAC 1 cut(s) 100
HpaII CCGG 2 cut(s) 191, 208
HphI GGTGA 3 cut(s) 515, 829, 880
Hpy166II GTNNAC 2 cut(s) 81, 100
Hpy188I TCNGA 5 cut(s) 75, 135, 226, 526, 799
Hpy188III TCNNGA 5 cut(s) 124, 335, 494, 699, 896
Hpy8I GTNNAC 2 cut(s) 81, 100
Hpy99I CGWCG 3 cut(s) 52, 55, 484
HpyAV CCTTC 1 cut(s) 629
HpyCH4III ACNGT 2 cut(s) 511, 580
HpyCH4IV ACGT 2 cut(s) 47, 720
HpyCH4V TGCA 4 cut(s) 16, 436, 556, 734
HpyF10VI GCNNNNNNNGC 8 cut(s) 176, 179, 208, 278, 325, 391, 590, 917
HpyF3I CTNAG 2 cut(s) 813, 927
HpySE526I ACGT 2 cut(s) 47, 720
Hsp92I GRCGYC 1 cut(s) 951
Hsp92II CATG 4 cut(s) 271, 385, 520, 662
KspAI GTTAAC 1 cut(s) 100
Kzo9I GATC 4 cut(s) 293, 445, 672, 879
Lsp1109I GCAGC 5 cut(s) 6, 28, 157, 166, 580
LweI GCATC 4 cut(s) 535, 543, 642, 696
MaeI CTAG 1 cut(s) 714
MaeII ACGT 2 cut(s) 47, 720
MaeIII GTNAC 3 cut(s) 351, 400, 716
MalI GATC 4 cut(s) 295, 447, 674, 881
MboI GATC 4 cut(s) 293, 445, 672, 879
MboII GAAGA 2 cut(s) 375, 837
MflI RGATCY 2 cut(s) 445, 879
MlsI TGGCCA 2 cut(s) 272, 779
MluCI AATT 5 cut(s) 220, 422, 475, 793, 860
MluNI TGGCCA 2 cut(s) 272, 779
MlyI GAGTC 2 cut(s) 221, 530
MmeI TCCRAC 1 cut(s) 249
Mox20I TGGCCA 2 cut(s) 272, 779
MroXI GAANNNNTTC 1 cut(s) 261
MscI TGGCCA 2 cut(s) 272, 779
MseI TTAA 2 cut(s) 99, 474
Msp20I TGGCCA 2 cut(s) 272, 779
MspA1I CMGCKG 2 cut(s) 19, 947
MspI CCGG 2 cut(s) 191, 208
MspR9I CCNGG 2 cut(s) 191, 322
MvaI CCWGG 1 cut(s) 322
MvnI CGCG 1 cut(s) 104
MwoI GCNNNNNNNGC 8 cut(s) 176, 179, 208, 278, 325, 391, 590, 917
NciI CCSGG 1 cut(s) 191
NdeII GATC 4 cut(s) 293, 445, 672, 879
NlaIII CATG 4 cut(s) 271, 385, 520, 662
NlaIV GGNNCC 4 cut(s) 150, 447, 487, 631
NmuCI GTSAC 1 cut(s) 716
NspI RCATGY 2 cut(s) 385, 662
PaeI GCATGC 1 cut(s) 385
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 127, 842
PflMI CCANNNNNTGG 1 cut(s) 698
PkrI GCNGC 6 cut(s) 18, 21, 172, 181, 213, 595
PleI GAGTC 2 cut(s) 221, 529
PpsI GAGTC 2 cut(s) 221, 529
PpuMI RGGWCCY 1 cut(s) 148
PshBI ATTAAT 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 4 cut(s) 150, 447, 487, 631
PspPI GGNCC 2 cut(s) 148, 318
PspPPI RGGWCCY 1 cut(s) 148
PsuI RGATCY 2 cut(s) 445, 879
PvuII CAGCTG 2 cut(s) 19, 947
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SaqAI TTAA 2 cut(s) 99, 474
SatI GCNGC 6 cut(s) 17, 20, 171, 180, 212, 594
Sau3AI GATC 4 cut(s) 293, 445, 672, 879
Sau96I GGNCC 2 cut(s) 148, 318
SchI GAGTC 2 cut(s) 221, 530
ScrFI CCNGG 2 cut(s) 191, 322
SfaNI GCATC 4 cut(s) 535, 543, 642, 696
SfcI CTRYAG 1 cut(s) 597
SinI GGWCC 1 cut(s) 148
SphI GCATGC 1 cut(s) 385
Sse9I AATT 5 cut(s) 220, 422, 475, 793, 860
SsiI CCGC 3 cut(s) 173, 212, 419
SspMI CTAG 1 cut(s) 714
StyD4I CCNGG 2 cut(s) 189, 320
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 511, 580
TaiI ACGT 2 cut(s) 50, 723
TaqI TCGA 2 cut(s) 161, 479
TasI AATT 5 cut(s) 220, 422, 475, 793, 860
TauI GCSGC 1 cut(s) 214
TfiI GAWTC 2 cut(s) 127, 842
Tru1I TTAA 2 cut(s) 99, 474
Tru9I TTAA 2 cut(s) 99, 474
TseFI GTSAC 1 cut(s) 716
TseI GCWGC 5 cut(s) 16, 19, 170, 179, 593
Tsp45I GTSAC 1 cut(s) 716
TspDTI ATGAA 2 cut(s) 270, 981
TspGWI ACGGA 2 cut(s) 85, 375
Van91I CCANNNNNTGG 1 cut(s) 698
VpaK11BI GGWCC 1 cut(s) 148
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 2 cut(s) 220, 793
XceI RCATGY 2 cut(s) 385, 662
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.