MD04G1200900.v1.1

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010248
Physical Location & Seq
Reverse (-)
28722051 .. 28725719
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1200900.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGGACAACATTTTGTGGGCTCTAAAAGGGCCAACAGAACATCCCCAACTTCTTCCCCACCACTACCAATCCAAATTCCCAGCCGAAGCTTCAAAGCCTTCCAAGTTCCGACTTCAACGAACCAACCACCAAGTATCTCGAATGGCGAGCAGTTTGCAGCTGCCAATCTCCATATCGTCAGCGTCTACGTCGTCGTTTAGACCCAGAAATGCTCGGAAGCTTACAGCTTTTTGCTCCGTTAACGCCAAAGCAACGGCCAAAATCCCAACTCCTCCAATAAACCCCAAGGACCCCTTTCTTTCGAGGCTCGCTGCGGTGGCTGCCAAGTCCCCGGAAACCTTGCTGAACCGGCCGCCTCAAAATTCCGACTCTCTCCCGTACTTGGATGTGTTTGATGAACCTATTCTCATGGCCACTCCTGCCCAAGTGGAAAGATCGGTATCTTACAACGAGCATAGGCCCAGGAAGCCACCTCCAGACCTGCCCTCGTTGTTACTCCACGGAAGAATAGTTTATATTGGCATGCCTTTAGTGCCAGCGGTCACAGAGCTTATTGTTGCGGAATTGATGTATCTGCAATGGATGGATCCTAAACAACCTATATATATTTATATTAATTCGACGGGAACCACCCGTGATGATGGTGAAACCGTTGGCATGGAGTCAGAGGGATTTGCCATCTATGATGCTCTGATGCAGATGAAGAATGAGATACATACGGTTGCCGTTGGGGCCGCTATAGGTCAAGCATGTCTATTGCTTTCAGCAGGAACTAAGGGCAAACGATTTATGATGCCACATGCCAAAGCAATGATCCAGCAACCTCGTGTCCCATCATCTGGACTGATGCCTGCTAGTGACGTTCTTATTCGTGCAAAAGAGGTAATCACAAATAGGGACATTCTCGTAAAGCTTTTGGCCAAACACACTGGAAATTCAGAGGAGGCTGTTGCTAAAGTGATGAGAAGACCATTTTATATGGATTCCACAACTGCTAAAGAATTCGGGGTGATTGACAAGATCCTTTGGCGTGGTCAAGAAAAGATAATGGCAGATGTAGCCTCCCCTGAGGATTGGGATAAGACAGCTGGCGTCAAAGTTGTAGATGAACTCGGTGGTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLP_protease

Protein Analysis

375

Amino Acids

41.3

Weight (kDa)

9.22

Isoelectric Point (pI)

51.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 163 - 342 1.7e-42 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 489
AccB7I CCANNNNNTGG 1 cut(s) 839
AccI GTMKAC 1 cut(s) 185
AciI CCGC 5 cut(s) 314, 353, 539, 560, 735
AclWI GGATC 4 cut(s) 581, 594, 808, 1015
AcoI YGGCCR 4 cut(s) 255, 350, 411, 918
AcsI RAATTY 4 cut(s) 74, 361, 934, 1001
AcyI GRCGYC 1 cut(s) 1092
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 2 cut(s) 382, 839
AgsI TTSAA 2 cut(s) 93, 116
AjnI CCWGG 1 cut(s) 461
AluBI AGCT 7 cut(s) 89, 160, 220, 227, 550, 913, 1088
AluI AGCT 7 cut(s) 89, 160, 220, 227, 550, 913, 1088
AlwI GGATC 4 cut(s) 581, 594, 808, 1015
AoxI GGCC 7 cut(s) 29, 255, 350, 411, 458, 732, 918
ApeKI GCWGC 4 cut(s) 157, 160, 311, 320
ApoI RAATTY 4 cut(s) 74, 361, 934, 1001
AseI ATTAAT 1 cut(s) 615
Asp700I GAANNNNTTC 1 cut(s) 402
AspS9I GGNCC 4 cut(s) 29, 289, 459, 732
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 656, 1021
AvaII GGWCC 1 cut(s) 289
AxyI CCTNAGG 1 cut(s) 1068
BalI TGGCCA 2 cut(s) 413, 920
BamHI GGATCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 22
BauI CACGAG 1 cut(s) 825
BbsI GAAGAC 1 cut(s) 973
BbvI GCAGC 4 cut(s) 147, 169, 298, 307
BccI CCATC 4 cut(s) 577, 635, 686, 841
BceAI ACGGC 2 cut(s) 270, 710
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BciT130I CCWGG 1 cut(s) 463
BcnI CCSGG 1 cut(s) 332
BfaI CTAG 1 cut(s) 855
BfmI CTRYAG 1 cut(s) 738
BfuAI ACCTGC 1 cut(s) 489
BglI GCCNNNNNGGC 1 cut(s) 731
BisI GCNGC 6 cut(s) 158, 161, 312, 321, 353, 735
BlsI GCNGC 6 cut(s) 159, 162, 313, 322, 354, 736
Bme1390I CCNGG 2 cut(s) 332, 463
Bme18I GGWCC 1 cut(s) 289
BmgT120I GGNCC 4 cut(s) 29, 289, 459, 732
BmiI GGNNCC 4 cut(s) 291, 588, 628, 733
BmrFI CCNGG 2 cut(s) 332, 463
BmsI GCATC 4 cut(s) 676, 684, 783, 837
BpiI GAAGAC 1 cut(s) 973
BpmI CTGGAG 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 332
BsaBI GATNNNNATC 1 cut(s) 439
BsaHI GRCGYC 1 cut(s) 1092
BsaJI CCNNGG 4 cut(s) 285, 330, 461, 499
Bsc4I CCNNNNNNNGG 2 cut(s) 382, 839
Bse118I RCCGGY 1 cut(s) 348
Bse1I ACTGG 1 cut(s) 934
Bse21I CCTNAGG 1 cut(s) 1068
Bse3DI GCAATG 2 cut(s) 584, 816
Bse8I GATNNNNATC 1 cut(s) 439
BseBI CCWGG 1 cut(s) 463
BseDI CCNNGG 4 cut(s) 285, 330, 461, 499
BseGI GGATG 3 cut(s) 40, 391, 588
BseJI GATNNNNATC 1 cut(s) 439
BseLI CCNNNNNNNGG 2 cut(s) 382, 839
BseMI GCAATG 2 cut(s) 584, 816
BseMII CTCAG 1 cut(s) 1059
BseNI ACTGG 1 cut(s) 934
BseRI GAGGAG 2 cut(s) 261, 956
BseX3I CGGCCG 1 cut(s) 350
BseXI GCAGC 4 cut(s) 147, 169, 298, 307
BseYI CCCAGC 1 cut(s) 79
Bsh1285I CGRYCG 1 cut(s) 353
BshFI GGCC 7 cut(s) 31, 257, 352, 413, 460, 734, 920
BsiEI CGRYCG 1 cut(s) 353
BsiSI CCGG 2 cut(s) 332, 349
BslFI GGGAC 3 cut(s) 313, 815, 911
BslI CCNNNNNNNGG 2 cut(s) 382, 839
BsmFI GGGAC 3 cut(s) 313, 815, 911
BsnI GGCC 7 cut(s) 31, 257, 352, 413, 460, 734, 920
Bsp1286I GDGCHC 1 cut(s) 22
Bsp143I GATC 4 cut(s) 434, 586, 813, 1020
BspACI CCGC 5 cut(s) 314, 353, 539, 560, 735
BspANI GGCC 7 cut(s) 31, 257, 352, 413, 460, 734, 920
BspCNI CTCAG 1 cut(s) 1060
BspLI GGNNCC 4 cut(s) 291, 588, 628, 733
BspMI ACCTGC 1 cut(s) 489
BspPI GGATC 4 cut(s) 581, 594, 808, 1015
BsrDI GCAATG 2 cut(s) 584, 816
BsrFI RCCGGY 1 cut(s) 348
BsrI ACTGG 1 cut(s) 934
BssAI RCCGGY 1 cut(s) 348
BssECI CCNNGG 4 cut(s) 285, 330, 461, 499
BssMI GATC 4 cut(s) 434, 586, 813, 1020
BssNI GRCGYC 1 cut(s) 1092
BssSI CACGAG 1 cut(s) 825
BssT1I CCWWGG 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 825
Bst2UI CCWGG 1 cut(s) 463
Bst4CI ACNGT 2 cut(s) 652, 721
BstACI GRCGYC 1 cut(s) 1092
BstC8I GCNNGC 6 cut(s) 148, 309, 524, 537, 852, 1090
BstDEI CTNAG 2 cut(s) 774, 1068
BstDSI CCRYGG 1 cut(s) 499
BstF5I GGATG 3 cut(s) 40, 391, 588
BstKTI GATC 4 cut(s) 437, 589, 816, 1023
BstMBI GATC 4 cut(s) 434, 586, 813, 1020
BstMCI CGRYCG 1 cut(s) 353
BstMWI GCNNNNNNNGC 8 cut(s) 317, 320, 349, 419, 466, 532, 731, 1058
BstNI CCWGG 1 cut(s) 463
BstNSI RCATGY 3 cut(s) 526, 753, 803
BstSCI CCNGG 2 cut(s) 330, 461
BstSFI CTRYAG 1 cut(s) 738
BstV1I GCAGC 4 cut(s) 147, 169, 298, 307
BstV2I GAAGAC 1 cut(s) 973
BstX2I RGATCY 2 cut(s) 586, 1020
BstYI RGATCY 2 cut(s) 586, 1020
BstZI CGGCCG 1 cut(s) 350
Bsu36I CCTNAGG 1 cut(s) 1068
BsuRI GGCC 7 cut(s) 31, 257, 352, 413, 460, 734, 920
BtgI CCRYGG 1 cut(s) 499
BtsCI GGATG 3 cut(s) 40, 391, 588
BtsIMutI CAGTG 1 cut(s) 927
BveI ACCTGC 1 cut(s) 489
Cac8I GCNNGC 6 cut(s) 148, 309, 524, 537, 852, 1090
Cfr10I RCCGGY 1 cut(s) 348
Cfr13I GGNCC 4 cut(s) 29, 289, 459, 732
CseI GACGC 2 cut(s) 171, 1081
Csp6I GTAC 1 cut(s) 379
CviAII CATG 5 cut(s) 409, 523, 658, 750, 800
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 2 cut(s) 774, 1068
DpnI GATC 4 cut(s) 436, 588, 815, 1022
DpnII GATC 4 cut(s) 434, 586, 813, 1020
EaeI YGGCCR 4 cut(s) 255, 350, 411, 918
EagI CGGCCG 1 cut(s) 350
EclXI CGGCCG 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 22
Eco47I GGWCC 1 cut(s) 289
Eco52I CGGCCG 1 cut(s) 350
Eco81I CCTNAGG 1 cut(s) 1068
EcoO109I RGGNCCY 1 cut(s) 289
EcoRI GAATTC 1 cut(s) 1001
EcoRII CCWGG 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 5 cut(s) 412, 526, 661, 753, 803
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FaqI GGGAC 3 cut(s) 313, 815, 911
FatI CATG 5 cut(s) 408, 522, 657, 749, 799
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 6 cut(s) 158, 161, 312, 321, 353, 735
FokI GGATG 3 cut(s) 27, 398, 595
FriOI GRGCYC 1 cut(s) 22
Fsp4HI GCNGC 6 cut(s) 158, 161, 312, 321, 353, 735
FspBI CTAG 1 cut(s) 855
GluI GCNGC 6 cut(s) 158, 161, 312, 321, 353, 735
GsaI CCCAGC 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 7 cut(s) 31, 257, 352, 413, 460, 734, 920
HapII CCGG 2 cut(s) 332, 349
HgaI GACGC 2 cut(s) 171, 1081
Hin1I GRCGYC 1 cut(s) 1092
Hin1II CATG 5 cut(s) 412, 526, 661, 753, 803
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HindIII AAGCTT 3 cut(s) 87, 218, 911
HinfI GANTC 3 cut(s) 368, 662, 983
HpaI GTTAAC 1 cut(s) 241
HpaII CCGG 2 cut(s) 332, 349
HphI GGTGA 2 cut(s) 656, 1021
Hpy166II GTNNAC 2 cut(s) 186, 241
Hpy188I TCNGA 6 cut(s) 110, 216, 367, 667, 693, 940
Hpy188III TCNNGA 4 cut(s) 138, 476, 840, 1037
Hpy8I GTNNAC 2 cut(s) 186, 241
Hpy99I CGWCG 3 cut(s) 193, 196, 625
HpyAV CCTTC 1 cut(s) 108
HpyCH4III ACNGT 2 cut(s) 652, 721
HpyCH4IV ACGT 2 cut(s) 188, 861
HpyCH4V TGCA 4 cut(s) 157, 577, 697, 875
HpyF10VI GCNNNNNNNGC 8 cut(s) 317, 320, 349, 419, 466, 532, 731, 1058
HpyF3I CTNAG 2 cut(s) 774, 1068
HpySE526I ACGT 2 cut(s) 188, 861
Hsp92I GRCGYC 1 cut(s) 1092
Hsp92II CATG 5 cut(s) 412, 526, 661, 753, 803
KspAI GTTAAC 1 cut(s) 241
Kzo9I GATC 4 cut(s) 434, 586, 813, 1020
LmnI GCTCC 1 cut(s) 239
Lsp1109I GCAGC 4 cut(s) 147, 169, 298, 307
LweI GCATC 4 cut(s) 676, 684, 783, 837
MaeI CTAG 1 cut(s) 855
MaeII ACGT 2 cut(s) 188, 861
MaeIII GTNAC 3 cut(s) 492, 541, 857
MalI GATC 4 cut(s) 436, 588, 815, 1022
MboI GATC 4 cut(s) 434, 586, 813, 1020
MboII GAAGA 4 cut(s) 44, 516, 715, 978
MflI RGATCY 2 cut(s) 586, 1020
MhlI GDGCHC 1 cut(s) 22
MlsI TGGCCA 2 cut(s) 413, 920
MluCI AATT 6 cut(s) 74, 361, 563, 616, 934, 1001
MluNI TGGCCA 2 cut(s) 413, 920
MlyI GAGTC 2 cut(s) 362, 671
MmeI TCCRAC 2 cut(s) 133, 390
Mox20I TGGCCA 2 cut(s) 413, 920
MroXI GAANNNNTTC 1 cut(s) 402
MscI TGGCCA 2 cut(s) 413, 920
MseI TTAA 2 cut(s) 240, 615
Msp20I TGGCCA 2 cut(s) 413, 920
MspA1I CMGCKG 3 cut(s) 160, 539, 1088
MspI CCGG 2 cut(s) 332, 349
MspR9I CCNGG 2 cut(s) 332, 463
MvaI CCWGG 1 cut(s) 463
MwoI GCNNNNNNNGC 8 cut(s) 317, 320, 349, 419, 466, 532, 731, 1058
NciI CCSGG 1 cut(s) 332
NdeII GATC 4 cut(s) 434, 586, 813, 1020
NlaIII CATG 5 cut(s) 412, 526, 661, 753, 803
NlaIV GGNNCC 4 cut(s) 291, 588, 628, 733
NmuCI GTSAC 2 cut(s) 541, 857
NspI RCATGY 3 cut(s) 526, 753, 803
PaeI GCATGC 1 cut(s) 526
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 1 cut(s) 983
PflMI CCANNNNNTGG 1 cut(s) 839
PkrI GCNGC 6 cut(s) 159, 162, 313, 322, 354, 736
PleI GAGTC 2 cut(s) 362, 670
PpsI GAGTC 2 cut(s) 362, 670
PpuMI RGGWCCY 1 cut(s) 289
PshBI ATTAAT 1 cut(s) 615
Psp5II RGGWCCY 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 461
PspFI CCCAGC 1 cut(s) 79
PspGI CCWGG 1 cut(s) 461
PspN4I GGNNCC 4 cut(s) 291, 588, 628, 733
PspPI GGNCC 4 cut(s) 29, 289, 459, 732
PspPPI RGGWCCY 1 cut(s) 289
PsuI RGATCY 2 cut(s) 586, 1020
PvuII CAGCTG 2 cut(s) 160, 1088
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
SaqAI TTAA 2 cut(s) 240, 615
SatI GCNGC 6 cut(s) 158, 161, 312, 321, 353, 735
Sau3AI GATC 4 cut(s) 434, 586, 813, 1020
Sau96I GGNCC 4 cut(s) 29, 289, 459, 732
SchI GAGTC 2 cut(s) 362, 671
ScrFI CCNGG 2 cut(s) 332, 463
SduI GDGCHC 1 cut(s) 22
SfaNI GCATC 4 cut(s) 676, 684, 783, 837
SfcI CTRYAG 1 cut(s) 738
SinI GGWCC 1 cut(s) 289
SphI GCATGC 1 cut(s) 526
Sse9I AATT 6 cut(s) 74, 361, 563, 616, 934, 1001
SsiI CCGC 5 cut(s) 314, 353, 539, 560, 735
SspMI CTAG 1 cut(s) 855
StyD4I CCNGG 2 cut(s) 330, 461
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 2 cut(s) 652, 721
TaiI ACGT 2 cut(s) 191, 864
TaqI TCGA 3 cut(s) 139, 302, 620
TasI AATT 6 cut(s) 74, 361, 563, 616, 934, 1001
TauI GCSGC 2 cut(s) 355, 737
TfiI GAWTC 1 cut(s) 983
Tru1I TTAA 2 cut(s) 240, 615
Tru9I TTAA 2 cut(s) 240, 615
TscAI CASTG 1 cut(s) 934
TseFI GTSAC 2 cut(s) 541, 857
TseI GCWGC 4 cut(s) 157, 160, 311, 320
Tsp45I GTSAC 2 cut(s) 541, 857
TspDTI ATGAA 3 cut(s) 411, 716, 1122
TspGWI ACGGA 2 cut(s) 226, 516
TspRI CASTG 1 cut(s) 934
Van91I CCANNNNNTGG 1 cut(s) 839
VpaK11BI GGWCC 1 cut(s) 289
VspI ATTAAT 1 cut(s) 615
XapI RAATTY 4 cut(s) 74, 361, 934, 1001
XceI RCATGY 3 cut(s) 526, 753, 803
XmiI GTMKAC 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 1 cut(s) 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.