FvH4_6g07450

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013902
Physical Location & Seq
Forward (+)
4462006 .. 4463626
1621 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07450.t1

Sequence Viewer

Length: 996 bp
ATGTGCTGCCATGGCGATGCTTTGGTCCAAAGAGTTTGCCAACTCTACGAGCAAATCTCAAGCCTCGAGAGCCTCAAACCCTCCCAAGATGTCAACACTCTCTTCACCCAACTCGTTCTCACATGCATGCCACCAAATCCAATCGATGTCACCAAGCTATGCAGAAGTGTGCAAGAAATAAGATCCAACCTCATAAGACTCTGCGGAGAAGCAGAAGGACTCTTGGAGTCTCATTTCTCCACCATCCTCGGCTCCTACGAATACCCTCTTCACCACCTCGACATTTTCCCTTACTACTCCAACTACCTCAAGCTGAGCAAACTCGAGCACTCAATCCTCACCCAACACTTCCCTCATGTACCTTCGAAAATCGCCTTTGTGGGTTCTGGCCCTCTTCCCCTTACCTCGATTGTTTTGGCCTCAAAACACCTCACCACCACCTCTTTTCATAACTACGATATCGACCCTTTGGCCAATTCCAAGGCTCTTGGCTTGGTTGCATCTGATCCTGACCTGTCCAAGAGGATGGTGTTCCATACTACTGACATAATGGATGTCACGAGTGATTTGAAGGAGTATGAGGTGGTGTTTCTTGCTGCTCTAGTTGGGATGGACAGGTCTGCGAAGGTGAAGATCATTGAGCACTTGGCCGAGAACATGAATCCCGGAGCGATTTTGATGCTGAGGAGTGCTCATGGTGCTAGGGCTTTTCTGTATCCGGTGATTGACCCTTGTGATGATCTTATAGGGTTTGAGGTTCTCTCTGTGTTTCACCCTACGGATGAAGTTATCAACTCGGTTGTGATTGCCCGGAGATCCTCGTATGCACCGCCGGCAGGGATACACTCATCGCCGCTCGATCAGAAGTCTGGGTTTAATGTTGGTTCTGTAAAGCTTCCCAACAAGTGCTGCTCTGAGGTTGAAGCCTTCAATCCTCTCAACCATGGCAACAATATGATCGAGGAATTGGTCTTTGATCAGGAGCAGCTTTCATAA

Protein Analysis

332

Amino Acids

36.63

Weight (kDa)

5.54

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 6 - 273 4e-131 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013902)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26483
fragaria_vesca FvH4_6g07450
malus_domestica MD04G1215600.v1.1 MD12G1205900.v1.1
prunus_persica Prupe.6G313000_v2.0.a1
pyrus_communis pycom04g17080 pycom12g19380
rosa_chinensis RchiOBHm_Chr3g0457051
rosa_laevigata RLG00000025248
rosa_multiflora Rmu_sc0000488.1_g000004
rosa_roxburghii Rroxscaffold_6G00422230
rosa_rugosa Rorug03G0018800
rosa_samantha Rh3AG078600 Rh3BG080400 Rh3CG080400 Rh3DG082200
rosa_wichuraiana Rw3G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 856
AciI CCGC 3 cut(s) 204, 830, 854
AclWI GGATC 3 cut(s) 177, 500, 810
AcoI YGGCCR 2 cut(s) 471, 648
AfaI GTAC 1 cut(s) 360
AfiI CCNNNNNNNGG 1 cut(s) 836
AgsI TTSAA 3 cut(s) 571, 923, 931
AluBI AGCT 4 cut(s) 157, 313, 895, 988
AluI AGCT 4 cut(s) 157, 313, 895, 988
Alw21I GWGCWC 3 cut(s) 330, 645, 694
Alw26I GTCTC 1 cut(s) 234
AlwI GGATC 3 cut(s) 177, 500, 810
Ama87I CYCGRG 2 cut(s) 65, 323
AoxI GGCC 4 cut(s) 388, 417, 471, 648
ApeKI GCWGC 4 cut(s) 6, 596, 909, 985
AspS9I GGNCC 2 cut(s) 25, 389
AsuC2I CCSGG 2 cut(s) 666, 811
AsuHPI GGTGA 8 cut(s) 97, 142, 263, 331, 424, 640, 733, 764
AsuII TTCGAA 1 cut(s) 365
AvaI CYCGRG 2 cut(s) 65, 323
AvaII GGWCC 1 cut(s) 25
BalI TGGCCA 1 cut(s) 473
BauI CACGAG 1 cut(s) 559
Bbv12I GWGCWC 3 cut(s) 330, 645, 694
BbvCI CCTCAGC 1 cut(s) 683
BbvI GCAGC 2 cut(s) 583, 896
BccI CCATC 3 cut(s) 251, 520, 604
BcgI CGANNNNNNTGC 2 cut(s) 661, 695
BciVI GTATCC 2 cut(s) 726, 834
BclI TGATCA 1 cut(s) 976
BcnI CCSGG 2 cut(s) 666, 811
BcoDI GTCTC 1 cut(s) 234
BfaI CTAG 2 cut(s) 602, 702
BfuI GTATCC 2 cut(s) 726, 834
BisI GCNGC 5 cut(s) 7, 597, 854, 910, 986
BlpI GCTNAGC 1 cut(s) 314
BlsI GCNGC 5 cut(s) 8, 598, 855, 911, 987
Bme1390I CCNGG 2 cut(s) 666, 811
Bme18I GGWCC 1 cut(s) 25
BmeT110I CYCGRG 2 cut(s) 65, 323
BmgT120I GGNCC 2 cut(s) 25, 389
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 2 cut(s) 666, 811
BmsI GCATC 3 cut(s) 7, 509, 669
BplI GAGNNNNNCTC 6 cut(s) 41, 73, 676, 708, 746, 778
Bpu10I CCTNAGC 1 cut(s) 683
Bpu1102I GCTNAGC 1 cut(s) 314
Bpu14I TTCGAA 1 cut(s) 365
BpuEI CTTGAG 2 cut(s) 43, 293
BpuMI CCSGG 2 cut(s) 666, 811
Bsa29I ATCGAT 1 cut(s) 144
BsaJI CCNNGG 4 cut(s) 10, 247, 480, 943
BsaWI WCCGGW 1 cut(s) 718
Bsc4I CCNNNNNNNGG 1 cut(s) 836
Bse118I RCCGGY 1 cut(s) 832
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 4 cut(s) 10, 247, 480, 943
BseGI GGATG 5 cut(s) 243, 531, 559, 615, 787
BseLI CCNNNNNNNGG 1 cut(s) 836
BseMII CTCAG 3 cut(s) 305, 674, 906
BseRI GAGGAG 1 cut(s) 700
BseXI GCAGC 2 cut(s) 583, 896
BshFI GGCC 4 cut(s) 390, 419, 473, 650
BshVI ATCGAT 1 cut(s) 144
BsiHKAI GWGCWC 3 cut(s) 330, 645, 694
BsiHKCI CYCGRG 2 cut(s) 65, 323
BsiSI CCGG 4 cut(s) 666, 719, 811, 833
BslI CCNNNNNNNGG 1 cut(s) 836
BsmAI GTCTC 1 cut(s) 234
BsnI GGCC 4 cut(s) 390, 419, 473, 650
BsoBI CYCGRG 2 cut(s) 65, 323
Bsp119I TTCGAA 1 cut(s) 365
Bsp1286I GDGCHC 3 cut(s) 330, 645, 694
Bsp143I GATC 8 cut(s) 182, 505, 633, 739, 815, 859, 957, 976
Bsp1720I GCTNAGC 1 cut(s) 314
Bsp19I CCATGG 2 cut(s) 10, 943
BspACI CCGC 3 cut(s) 204, 830, 854
BspANI GGCC 4 cut(s) 390, 419, 473, 650
BspCNI CTCAG 3 cut(s) 306, 675, 907
BspDI ATCGAT 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 253
BspPI GGATC 3 cut(s) 177, 500, 810
BspT104I TTCGAA 1 cut(s) 365
BsrBI CCGCTC 1 cut(s) 856
BsrFI RCCGGY 1 cut(s) 832
BssAI RCCGGY 1 cut(s) 832
BssECI CCNNGG 4 cut(s) 10, 247, 480, 943
BssMI GATC 8 cut(s) 182, 505, 633, 739, 815, 859, 957, 976
BssSI CACGAG 1 cut(s) 559
BssT1I CCWWGG 3 cut(s) 10, 480, 943
Bst2BI CACGAG 1 cut(s) 559
Bst6I CTCTTC 3 cut(s) 107, 273, 399
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 2 cut(s) 128, 834
BstDEI CTNAG 3 cut(s) 314, 683, 915
BstDSI CCRYGG 2 cut(s) 10, 943
BstF5I GGATG 5 cut(s) 243, 531, 559, 615, 787
BstKTI GATC 8 cut(s) 185, 508, 636, 742, 818, 862, 960, 979
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 8 cut(s) 182, 505, 633, 739, 815, 859, 957, 976
BstMWI GCNNNNNNNGC 4 cut(s) 12, 69, 698, 833
BstNSI RCATGY 2 cut(s) 126, 130
BstSCI CCNGG 2 cut(s) 664, 809
BstV1I GCAGC 2 cut(s) 583, 896
BstX2I RGATCY 2 cut(s) 182, 815
BstXI CCANNNNNNTGG 1 cut(s) 526
BstYI RGATCY 2 cut(s) 182, 815
Bsu15I ATCGAT 1 cut(s) 144
BsuI GTATCC 2 cut(s) 726, 834
BsuRI GGCC 4 cut(s) 390, 419, 473, 650
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 2 cut(s) 10, 943
BtgZI GCGATG 2 cut(s) 30, 834
BtsCI GGATG 5 cut(s) 243, 531, 559, 615, 787
Cac8I GCNNGC 2 cut(s) 128, 834
Cfr10I RCCGGY 1 cut(s) 832
Cfr13I GGNCC 2 cut(s) 25, 389
ClaI ATCGAT 1 cut(s) 144
Csp6I GTAC 1 cut(s) 359
CviAII CATG 7 cut(s) 11, 123, 127, 356, 658, 695, 944
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 3 cut(s) 314, 683, 915
DpnI GATC 8 cut(s) 184, 507, 635, 741, 817, 861, 959, 978
DpnII GATC 8 cut(s) 182, 505, 633, 739, 815, 859, 957, 976
EaeI YGGCCR 2 cut(s) 471, 648
Eam1104I CTCTTC 3 cut(s) 107, 273, 399
EarI CTCTTC 3 cut(s) 107, 273, 399
Eco130I CCWWGG 3 cut(s) 10, 480, 943
Eco32I GATATC 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 25
Eco88I CYCGRG 2 cut(s) 65, 323
EcoRV GATATC 1 cut(s) 460
EcoT14I CCWWGG 3 cut(s) 10, 480, 943
EcoT22I ATGCAT 1 cut(s) 128
ErhI CCWWGG 3 cut(s) 10, 480, 943
FaeI CATG 7 cut(s) 14, 126, 130, 359, 661, 698, 947
FatI CATG 7 cut(s) 10, 122, 126, 355, 657, 694, 943
FbaI TGATCA 1 cut(s) 976
Fnu4HI GCNGC 5 cut(s) 7, 597, 854, 910, 986
FokI GGATG 5 cut(s) 230, 538, 566, 622, 794
Fsp4HI GCNGC 5 cut(s) 7, 597, 854, 910, 986
FspBI CTAG 2 cut(s) 602, 702
GluI GCNGC 5 cut(s) 7, 597, 854, 910, 986
HaeIII GGCC 4 cut(s) 390, 419, 473, 650
HapII CCGG 4 cut(s) 666, 719, 811, 833
Hin1II CATG 7 cut(s) 14, 126, 130, 359, 661, 698, 947
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 4 cut(s) 198, 219, 227, 661
HpaII CCGG 4 cut(s) 666, 719, 811, 833
HphI GGTGA 8 cut(s) 97, 142, 263, 331, 424, 640, 733, 764
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 3 cut(s) 505, 864, 916
Hpy188III TCNNGA 4 cut(s) 67, 509, 559, 980
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 5 cut(s) 209, 372, 565, 619, 937
HpyCH4V TGCA 5 cut(s) 126, 162, 172, 500, 827
HpyF10VI GCNNNNNNNGC 4 cut(s) 12, 69, 698, 833
HpyF3I CTNAG 3 cut(s) 314, 683, 915
Hsp92II CATG 7 cut(s) 14, 126, 130, 359, 661, 698, 947
KroI GCCGGC 1 cut(s) 832
KroNI GCCGGC 1 cut(s) 834
Ksp22I TGATCA 1 cut(s) 976
Kzo9I GATC 8 cut(s) 182, 505, 633, 739, 815, 859, 957, 976
LmnI GCTCC 3 cut(s) 257, 668, 982
Lsp1109I GCAGC 2 cut(s) 583, 896
LweI GCATC 3 cut(s) 7, 509, 669
MaeI CTAG 2 cut(s) 602, 702
MaeIII GTNAC 2 cut(s) 148, 556
MalI GATC 8 cut(s) 184, 507, 635, 741, 817, 861, 959, 978
MbiI CCGCTC 1 cut(s) 856
MboI GATC 8 cut(s) 182, 505, 633, 739, 815, 859, 957, 976
MboII GAAGA 4 cut(s) 94, 260, 386, 643
MflI RGATCY 2 cut(s) 182, 815
MhlI GDGCHC 3 cut(s) 330, 645, 694
MlsI TGGCCA 1 cut(s) 473
MluCI AATT 2 cut(s) 475, 965
MluNI TGGCCA 1 cut(s) 473
MlyI GAGTC 3 cut(s) 192, 213, 236
MmeI TCCRAC 2 cut(s) 210, 324
Mox20I TGGCCA 1 cut(s) 473
Mph1103I ATGCAT 1 cut(s) 128
MroNI GCCGGC 1 cut(s) 832
MscI TGGCCA 1 cut(s) 473
MseI TTAA 1 cut(s) 876
MslI CAYNNNNRTG 2 cut(s) 15, 125
Msp20I TGGCCA 1 cut(s) 473
MspI CCGG 4 cut(s) 666, 719, 811, 833
MspR9I CCNGG 2 cut(s) 666, 811
MwoI GCNNNNNNNGC 4 cut(s) 12, 69, 698, 833
NaeI GCCGGC 1 cut(s) 834
NciI CCSGG 2 cut(s) 666, 811
NcoI CCATGG 2 cut(s) 10, 943
NdeII GATC 8 cut(s) 182, 505, 633, 739, 815, 859, 957, 976
NgoMIV GCCGGC 1 cut(s) 832
NlaIII CATG 7 cut(s) 14, 126, 130, 359, 661, 698, 947
NlaIV GGNNCC 1 cut(s) 253
NmeAIII GCCGAG 2 cut(s) 228, 676
NmuCI GTSAC 2 cut(s) 148, 556
NsiI ATGCAT 1 cut(s) 128
NspI RCATGY 2 cut(s) 126, 130
NspV TTCGAA 1 cut(s) 365
PaeI GCATGC 1 cut(s) 130
PaeR7I CTCGAG 2 cut(s) 65, 323
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdiI GCCGGC 1 cut(s) 834
PfeI GAWTC 1 cut(s) 661
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 5 cut(s) 8, 598, 855, 911, 987
PleI GAGTC 3 cut(s) 192, 213, 235
PpsI GAGTC 3 cut(s) 192, 213, 235
PspN4I GGNNCC 1 cut(s) 253
PspPI GGNCC 2 cut(s) 25, 389
PspXI VCTCGAGB 1 cut(s) 323
PsuI RGATCY 2 cut(s) 182, 815
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
RseI CAYNNNNRTG 2 cut(s) 15, 125
SaqAI TTAA 1 cut(s) 876
SatI GCNGC 5 cut(s) 7, 597, 854, 910, 986
Sau3AI GATC 8 cut(s) 182, 505, 633, 739, 815, 859, 957, 976
Sau96I GGNCC 2 cut(s) 25, 389
SchI GAGTC 3 cut(s) 192, 213, 236
ScrFI CCNGG 2 cut(s) 666, 811
SduI GDGCHC 3 cut(s) 330, 645, 694
SfaNI GCATC 3 cut(s) 7, 509, 669
Sfr274I CTCGAG 2 cut(s) 65, 323
SfuI TTCGAA 1 cut(s) 365
SinI GGWCC 1 cut(s) 25
SlaI CTCGAG 2 cut(s) 65, 323
SmiMI CAYNNNNRTG 2 cut(s) 15, 125
SmlI CTYRAG 4 cut(s) 58, 65, 308, 323
SmoI CTYRAG 4 cut(s) 58, 65, 308, 323
SphI GCATGC 1 cut(s) 130
Sse9I AATT 2 cut(s) 475, 965
SsiI CCGC 3 cut(s) 204, 830, 854
SspMI CTAG 2 cut(s) 602, 702
StyD4I CCNGG 2 cut(s) 664, 809
StyI CCWWGG 3 cut(s) 10, 480, 943
TaqI TCGA 9 cut(s) 66, 144, 279, 324, 365, 407, 462, 858, 960
TasI AATT 2 cut(s) 475, 965
TauI GCSGC 1 cut(s) 856
TfiI GAWTC 1 cut(s) 661
Tru1I TTAA 1 cut(s) 876
Tru9I TTAA 1 cut(s) 876
TseFI GTSAC 2 cut(s) 148, 556
TseI GCWGC 4 cut(s) 6, 596, 909, 985
Tsp45I GTSAC 2 cut(s) 148, 556
TspDTI ATGAA 4 cut(s) 437, 674, 798, 981
TspGWI ACGGA 1 cut(s) 794
VpaK11BI GGWCC 1 cut(s) 25
XceI RCATGY 2 cut(s) 126, 130
XhoI CTCGAG 2 cut(s) 65, 323
XspI CTAG 2 cut(s) 602, 702
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.