MD12G1205900.v1.1

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013902
Physical Location & Seq
Reverse (-)
28632794 .. 28633847
1054 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1205900.v1.1.491

Sequence Viewer

Length: 978 bp
ATGTGTTGCCAGGGAGATGCTTTGGTACAAAGAGTTTGCGAATTGTATGAGAAAATCTCAAGCCTTGAGAGCCTCAAACCCTCCCAAGATGTCGACATGCTCTTTACCCAACTCGTTCTCACATGCATACCACCAAGTCCAATCGATGTTTCCAAGTTATGCCAAGGGGTGCAAGAAATAAGGTCCAACCTTATAAGGCTATGTGGAGAGGCAGAAGGACTTTTGGAGAATCACTTCTCGACAATCCTAGGCTCATATGAACACCCTCTCGACCATCTCGACATTTTCCCTTACTATTCCAATTACCTCAAGCTTAGCAAACTTGAGTTCAACATCCTTAGCCAACACTTCACTCGTGTGCCTATAAAAATTGCCTTTGTGGGTTCTGGCCCTCTTCCCCTTACCTCAATTGTCTTGGCCTCAAATCACCTCACCAAAACCTCTTTCGACAACTATGACATCAATGCTTCGGCCAACTCCAAGGCCCTTGGCTTGGTGTCTTCCGATCCTGACCTATCGAAAAGAATGGTCTTCCACACCACAGATATAATGGATGTCACAACTGCTTTGAAAGACTATGAAATTGTGTTTTTGGCTGCTCTTGTAGGCATGGACAAGATGGAGAAGCTGAAAATTATCGATCACTTGGCAAAATACATGGCTCCAGGAGCAACTTTGATGCTAAGAAGTGCACACGGTGCGAGGGCTTTTCTATATCCAGTGATTGATCCTTGTGATGATCTTAGAGGGTTTGAGGTTCTCTCTGTGTTTCATCCCACCGATGAAGTCATTAACTCAATTGTCATCGCGCGTAAGTATGCATTGCCTGTTCAGCACTCATCACCAATTGATTCTCAGGGTCTTAATGCTGGTGCCATTATACTTCCCAGCAAGTGTTGTGAGCTTGAAGCCTTCAATCCCCTCCATAAACATGGCAACATGATTGAGGAGTTGGCCTTTGAGGAGCAGCTTTCTTAA

Protein Analysis

326

Amino Acids

36.03

Weight (kDa)

5.33

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 6 - 274 1.3e-136 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013902)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26483
fragaria_vesca FvH4_6g07450
malus_domestica MD04G1215600.v1.1 MD12G1205900.v1.1
prunus_persica Prupe.6G313000_v2.0.a1
pyrus_communis pycom04g17080 pycom12g19380
rosa_chinensis RchiOBHm_Chr3g0457051
rosa_laevigata RLG00000025248
rosa_multiflora Rmu_sc0000488.1_g000004
rosa_roxburghii Rroxscaffold_6G00422230
rosa_rugosa Rorug03G0018800
rosa_samantha Rh3AG078600 Rh3BG080400 Rh3CG080400 Rh3DG082200
rosa_wichuraiana Rw3G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 872
AccI GTMKAC 1 cut(s) 93
AccII CGCG 2 cut(s) 809, 811
AclWI GGATC 2 cut(s) 500, 722
AcoI YGGCCR 1 cut(s) 471
AdeI CACNNNGTG 1 cut(s) 698
AfaI GTAC 1 cut(s) 27
AfiI CCNNNNNNNGG 1 cut(s) 493
AgsI TTSAA 4 cut(s) 331, 571, 908, 916
AjnI CCWGG 2 cut(s) 9, 664
AjuI GAANNNNNNNTTGG 4 cut(s) 133, 165, 428, 460
AleI CACNNNNGTG 1 cut(s) 356
AluBI AGCT 4 cut(s) 313, 628, 904, 970
AluI AGCT 4 cut(s) 313, 628, 904, 970
Alw21I GWGCWC 1 cut(s) 694
Alw44I GTGCAC 1 cut(s) 690
AlwI GGATC 2 cut(s) 500, 722
AoxI GGCC 5 cut(s) 388, 417, 471, 483, 954
ApaLI GTGCAC 1 cut(s) 690
ApeKI GCWGC 2 cut(s) 596, 967
Asp700I GAANNNNTTC 1 cut(s) 233
AspA2I CCTAGG 1 cut(s) 247
AspLEI GCGC 1 cut(s) 811
AspS9I GGNCC 3 cut(s) 183, 389, 484
AsuHPI GGTGA 3 cut(s) 419, 424, 834
AvaII GGWCC 1 cut(s) 183
AvrII CCTAGG 1 cut(s) 247
BaeGI GKGCMC 1 cut(s) 694
BanI GGYRCC 1 cut(s) 872
BauI CACGAG 1 cut(s) 354
BbsI GAAGAC 2 cut(s) 492, 523
Bbv12I GWGCWC 1 cut(s) 694
BbvI GCAGC 1 cut(s) 583
BccI CCATC 2 cut(s) 282, 613
BciT130I CCWGG 2 cut(s) 11, 666
BfaI CTAG 1 cut(s) 248
BisI GCNGC 2 cut(s) 597, 968
BlnI CCTAGG 1 cut(s) 247
BlpI GCTNAGC 1 cut(s) 314
BlsI GCNGC 2 cut(s) 598, 969
Bme1390I CCNGG 2 cut(s) 11, 666
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 3 cut(s) 183, 389, 484
BmiI GGNNCC 2 cut(s) 663, 874
BmrFI CCNGG 2 cut(s) 11, 666
BmsI GCATC 2 cut(s) 7, 669
BpiI GAAGAC 2 cut(s) 492, 523
BplI GAGNNNNNCTC 4 cut(s) 41, 73, 746, 778
BpmI CTGGAG 1 cut(s) 648
Bpu10I CCTNAGC 1 cut(s) 338
Bpu1102I GCTNAGC 1 cut(s) 314
BpuEI CTTGAG 4 cut(s) 43, 86, 293, 344
Bsa29I ATCGAT 2 cut(s) 144, 639
BsaJI CCNNGG 5 cut(s) 10, 163, 247, 480, 487
Bsc4I CCNNNNNNNGG 1 cut(s) 493
Bse1I ACTGG 1 cut(s) 719
Bse3DI GCAATG 1 cut(s) 821
BseBI CCWGG 2 cut(s) 11, 666
BseCI ATCGAT 2 cut(s) 144, 639
BseDI CCNNGG 5 cut(s) 10, 163, 247, 480, 487
BseGI GGATG 3 cut(s) 333, 559, 772
BseLI CCNNNNNNNGG 1 cut(s) 493
BseMI GCAATG 1 cut(s) 821
BseMII CTCAG 1 cut(s) 869
BseNI ACTGG 1 cut(s) 719
BseRI GAGGAG 2 cut(s) 962, 977
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 1 cut(s) 583
BseYI CCCAGC 1 cut(s) 887
Bsh1236I CGCG 2 cut(s) 809, 811
BshFI GGCC 5 cut(s) 390, 419, 473, 485, 956
BshNI GGYRCC 1 cut(s) 872
BshVI ATCGAT 2 cut(s) 144, 639
BsiHKAI GWGCWC 1 cut(s) 694
BslI CCNNNNNNNGG 1 cut(s) 493
BsnI GGCC 5 cut(s) 390, 419, 473, 485, 956
Bsp1286I GDGCHC 1 cut(s) 694
Bsp143I GATC 4 cut(s) 505, 640, 727, 739
Bsp1720I GCTNAGC 1 cut(s) 314
BspANI GGCC 5 cut(s) 390, 419, 473, 485, 956
BspCNI CTCAG 1 cut(s) 868
BspDI ATCGAT 2 cut(s) 144, 639
BspFNI CGCG 2 cut(s) 809, 811
BspLI GGNNCC 2 cut(s) 663, 874
BspPI GGATC 2 cut(s) 500, 722
BspT107I GGYRCC 1 cut(s) 872
BsrDI GCAATG 1 cut(s) 821
BsrI ACTGG 1 cut(s) 719
BssECI CCNNGG 5 cut(s) 10, 163, 247, 480, 487
BssMI GATC 4 cut(s) 505, 640, 727, 739
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 4 cut(s) 163, 247, 480, 487
Bst2BI CACGAG 1 cut(s) 354
Bst2UI CCWGG 2 cut(s) 11, 666
Bst4CI ACNGT 1 cut(s) 698
Bst6I CTCTTC 1 cut(s) 399
BstAPI GCANNNNNTGC 1 cut(s) 698
BstDEI CTNAG 5 cut(s) 314, 338, 683, 743, 855
BstF5I GGATG 3 cut(s) 333, 559, 772
BstFNI CGCG 2 cut(s) 809, 811
BstHHI GCGC 1 cut(s) 811
BstKTI GATC 4 cut(s) 508, 643, 730, 742
BstMBI GATC 4 cut(s) 505, 640, 727, 739
BstMWI GCNNNNNNNGC 4 cut(s) 69, 668, 698, 832
BstNI CCWGG 2 cut(s) 11, 666
BstNSI RCATGY 2 cut(s) 100, 126
BstSCI CCNGG 2 cut(s) 9, 664
BstSLI GKGCMC 1 cut(s) 694
BstUI CGCG 2 cut(s) 809, 811
BstV1I GCAGC 1 cut(s) 583
BstV2I GAAGAC 2 cut(s) 492, 523
BstXI CCANNNNNNTGG 1 cut(s) 932
Bsu15I ATCGAT 2 cut(s) 144, 639
BsuRI GGCC 5 cut(s) 390, 419, 473, 485, 956
BsuTUI ATCGAT 2 cut(s) 144, 639
BtgZI GCGATG 1 cut(s) 790
BtsCI GGATG 3 cut(s) 333, 559, 772
BtsIMutI CAGTG 1 cut(s) 726
CfoI GCGC 1 cut(s) 811
Cfr13I GGNCC 3 cut(s) 183, 389, 484
ClaI ATCGAT 2 cut(s) 144, 639
Csp6I GTAC 1 cut(s) 26
CviAII CATG 6 cut(s) 97, 123, 610, 658, 932, 940
CviQI GTAC 1 cut(s) 26
DdeI CTNAG 5 cut(s) 314, 338, 683, 743, 855
DpnI GATC 4 cut(s) 507, 642, 729, 741
DpnII GATC 4 cut(s) 505, 640, 727, 739
DraIII CACNNNGTG 1 cut(s) 698
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Eco130I CCWWGG 4 cut(s) 163, 247, 480, 487
Eco47I GGWCC 1 cut(s) 183
EcoO109I RGGNCCY 1 cut(s) 484
EcoRII CCWGG 2 cut(s) 9, 664
EcoT14I CCWWGG 4 cut(s) 163, 247, 480, 487
EcoT22I ATGCAT 2 cut(s) 128, 823
ErhI CCWWGG 4 cut(s) 163, 247, 480, 487
FaeI CATG 6 cut(s) 100, 126, 613, 661, 935, 943
FatI CATG 6 cut(s) 96, 122, 609, 657, 931, 939
FauNDI CATATG 1 cut(s) 256
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 2 cut(s) 597, 968
FokI GGATG 3 cut(s) 320, 566, 759
Fsp4HI GCNGC 2 cut(s) 597, 968
FspBI CTAG 1 cut(s) 248
GlaI GCGC 1 cut(s) 810
GluI GCNGC 2 cut(s) 597, 968
GsaI CCCAGC 1 cut(s) 891
GsuI CTGGAG 1 cut(s) 648
HaeIII GGCC 5 cut(s) 390, 419, 473, 485, 956
HhaI GCGC 1 cut(s) 811
Hin1II CATG 6 cut(s) 100, 126, 613, 661, 935, 943
Hin6I GCGC 1 cut(s) 809
HinP1I GCGC 1 cut(s) 809
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 2 cut(s) 229, 851
HphI GGTGA 3 cut(s) 419, 424, 834
Hpy166II GTNNAC 2 cut(s) 94, 692
Hpy188I TCNGA 1 cut(s) 505
Hpy188III TCNNGA 4 cut(s) 238, 269, 278, 509
Hpy8I GTNNAC 2 cut(s) 94, 692
HpyAV CCTTC 2 cut(s) 209, 922
HpyCH4III ACNGT 1 cut(s) 698
HpyCH4V TGCA 4 cut(s) 126, 172, 692, 821
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 668, 698, 832
HpyF3I CTNAG 5 cut(s) 314, 338, 683, 743, 855
Hsp92II CATG 6 cut(s) 100, 126, 613, 661, 935, 943
HspAI GCGC 1 cut(s) 809
Kzo9I GATC 4 cut(s) 505, 640, 727, 739
LmnI GCTCC 3 cut(s) 667, 668, 964
Lsp1109I GCAGC 1 cut(s) 583
LweI GCATC 2 cut(s) 7, 669
MaeI CTAG 1 cut(s) 248
MaeIII GTNAC 1 cut(s) 556
MalI GATC 4 cut(s) 507, 642, 729, 741
MboI GATC 4 cut(s) 505, 640, 727, 739
MboII GAAGA 3 cut(s) 386, 492, 523
MfeI CAATTG 3 cut(s) 408, 798, 846
MhlI GDGCHC 1 cut(s) 694
MluCI AATT 8 cut(s) 41, 301, 369, 408, 582, 633, 798, 846
MmeI TCCRAC 1 cut(s) 210
Mph1103I ATGCAT 2 cut(s) 128, 823
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 3 cut(s) 792, 864, 976
MslI CAYNNNNRTG 2 cut(s) 356, 930
MspR9I CCNGG 2 cut(s) 11, 666
MunI CAATTG 3 cut(s) 408, 798, 846
MvaI CCWGG 2 cut(s) 11, 666
MvnI CGCG 2 cut(s) 809, 811
MwoI GCNNNNNNNGC 4 cut(s) 69, 668, 698, 832
NdeI CATATG 1 cut(s) 256
NdeII GATC 4 cut(s) 505, 640, 727, 739
NlaIII CATG 6 cut(s) 100, 126, 613, 661, 935, 943
NlaIV GGNNCC 2 cut(s) 663, 874
NmuCI GTSAC 1 cut(s) 556
NsiI ATGCAT 2 cut(s) 128, 823
NspI RCATGY 2 cut(s) 100, 126
OliI CACNNNNGTG 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 2 cut(s) 229, 851
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 2 cut(s) 598, 969
PsiI TTATAA 1 cut(s) 194
Psp6I CCWGG 2 cut(s) 9, 664
PspFI CCCAGC 1 cut(s) 887
PspGI CCWGG 2 cut(s) 9, 664
PspN4I GGNNCC 2 cut(s) 663, 874
PspPI GGNCC 3 cut(s) 183, 389, 484
RsaI GTAC 1 cut(s) 27
RsaNI GTAC 1 cut(s) 26
RseI CAYNNNNRTG 2 cut(s) 356, 930
SalI GTCGAC 1 cut(s) 92
SaqAI TTAA 3 cut(s) 792, 864, 976
SatI GCNGC 2 cut(s) 597, 968
Sau3AI GATC 4 cut(s) 505, 640, 727, 739
Sau96I GGNCC 3 cut(s) 183, 389, 484
ScrFI CCNGG 2 cut(s) 11, 666
SduI GDGCHC 1 cut(s) 694
SfaNI GCATC 2 cut(s) 7, 669
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 2 cut(s) 356, 930
SmlI CTYRAG 4 cut(s) 58, 65, 308, 323
SmoI CTYRAG 4 cut(s) 58, 65, 308, 323
Sse9I AATT 8 cut(s) 41, 301, 369, 408, 582, 633, 798, 846
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 9, 664
StyI CCWWGG 4 cut(s) 163, 247, 480, 487
TaaI ACNGT 1 cut(s) 698
TaqI TCGA 8 cut(s) 93, 144, 239, 270, 279, 447, 518, 639
TasI AATT 8 cut(s) 41, 301, 369, 408, 582, 633, 798, 846
TfiI GAWTC 2 cut(s) 229, 851
Tru1I TTAA 3 cut(s) 792, 864, 976
Tru9I TTAA 3 cut(s) 792, 864, 976
TscAI CASTG 1 cut(s) 726
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 2 cut(s) 596, 967
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 4 cut(s) 273, 594, 761, 798
TspRI CASTG 1 cut(s) 726
VneI GTGCAC 1 cut(s) 690
VpaK11BI GGWCC 1 cut(s) 183
XceI RCATGY 2 cut(s) 100, 126
XcmI CCANNNNNNNNNTGG 1 cut(s) 547
XmaJI CCTAGG 1 cut(s) 247
XmiI GTMKAC 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 1 cut(s) 248
Zsp2I ATGCAT 2 cut(s) 128, 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.