Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0013902

Physical Location & Seq

Genomic Coordinates Reverse (-)

29855488 .. 29856748

Gene Structure 1261 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1215600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 819 bp

ATGTGTTGCCAGGGAGATGCTTTGGTACAAAGAGTGTGTGAATTGTATGAGAAAATCTCAAGCCTAGAGAGCCTCAAACCCTCCCAAGATGTCAACATGCTCTTCACCCAACTCGTCCTGACATGCATACCACCAAGTCCAATCGATGTTTCCAAGTTATGCCGAGGGGTGCAAGAAATAAGGTCAAATCTTATAAGGCTTTGTGGAGAAGCAGAAGGACTTTTGGAGAATCACTTCTCAACAATCCTAGGCTCATTTGAGCACCCTCTCGACCATCTCGACATTTTCCCTTACTATTCCAATTACCTCAAGCTTAGCAAACTTGAGTTCAACATCCTTAGCCAACACTTCACCCGTGTGCCTAGCAAAATTGCCTTTGTGGGTTCTGGCCCTCTTCCCCTTACTTCAATTGTCTTGGCCTCCAATCACCTCACCAAAACCTCTTTTGACAACTATGACATCGACACATTGGCCAACTCAATGGCTCTTCGCTTAGTGTCATCCGATCCTGACCTATCAAAAAGGATGGTTGCACACGGCGCGAGAGCTTTTCTTTATCCGGTGATTGATCCTTGTGATGATCTTAGAGGGTTTGAGGTTCTCTCTGTGTTTCATCCCACCGATGAAGTCATTAATTCGATTGTCATCGCGCGTAAATACGCATTGCCTGTTCAGCACTCATCATCAATTGATTCTCAGGAGATTAATAATGCTGGTGCCATTAGACTTCCCAGCAAGTGTTGTGAGATTGAAGCCTTCAATCCTTTCCATAAACTTGGAAACATGATTGAGGAGTTGGCCTTCGAGGAGCAGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0006810 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010232 GO:0010233 GO:0016043 GO:0016049 GO:0022414 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0044706 GO:0048229 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051179 GO:0051234 GO:0051704 GO:0060560 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAS

Protein Analysis

273

Amino Acids

30.36

Weight (kDa)

5.24

Isoelectric Point (pI)

40.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAS	PF03059	6 - 179	7.5e-83	Nicotianamine synthase protein
NAS	PF03059	178 - 221	6e-16	Nicotianamine synthase protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013902)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26483
fragaria_vesca	FvH4_6g07450
malus_domestica	MD04G1215600.v1.1 MD12G1205900.v1.1
prunus_persica	Prupe.6G313000_v2.0.a1
pyrus_communis	pycom04g17080 pycom12g19380
rosa_chinensis	RchiOBHm_Chr3g0457051
rosa_laevigata	RLG00000025248
rosa_multiflora	Rmu_sc0000488.1_g000004
rosa_roxburghii	Rroxscaffold_6G00422230
rosa_rugosa	Rorug03G0018800
rosa_samantha	Rh3AG078600 Rh3BG080400 Rh3CG080400 Rh3DG082200
rosa_wichuraiana	Rw3G006750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	194
AccB1I	GGYRCC	1 cut(s)	716
AccII	CGCG	3 cut(s)	542, 650, 652
AclWI	GGATC	2 cut(s)	500, 563
AcoI	YGGCCR	1 cut(s)	471
AfaI	GTAC	1 cut(s)	27
AgsI	TTSAA	4 cut(s)	331, 408, 752, 760
AjnI	CCWGG	1 cut(s)	9
AjuI	GAANNNNNNNTTGG	2 cut(s)	133, 165
AleI	CACNNNNGTG	1 cut(s)	356
AluBI	AGCT	3 cut(s)	313, 548, 814
AluI	AGCT	3 cut(s)	313, 548, 814
Alw21I	GWGCWC	1 cut(s)	264
AlwI	GGATC	2 cut(s)	500, 563
AoxI	GGCC	4 cut(s)	388, 417, 471, 798
ApeKI	GCWGC	1 cut(s)	811
AseI	ATTAAT	2 cut(s)	633, 705
Asp700I	GAANNNNTTC	1 cut(s)	233
AspA2I	CCTAGG	1 cut(s)	247
AspLEI	GCGC	2 cut(s)	542, 652
AspS9I	GGNCC	1 cut(s)	389
AsuHPI	GGTGA	5 cut(s)	97, 343, 419, 424, 574
AvrII	CCTAGG	1 cut(s)	247
BalI	TGGCCA	1 cut(s)	473
BanI	GGYRCC	1 cut(s)	716
Bbv12I	GWGCWC	1 cut(s)	264
BccI	CCATC	2 cut(s)	282, 520
BceAI	ACGGC	1 cut(s)	553
BciT130I	CCWGG	1 cut(s)	11
BfaI	CTAG	3 cut(s)	65, 248, 363
BisI	GCNGC	1 cut(s)	812
BlnI	CCTAGG	1 cut(s)	247
BlpI	GCTNAGC	1 cut(s)	314
BlsI	GCNGC	1 cut(s)	813
Bme1390I	CCNGG	1 cut(s)	11
BmgT120I	GGNCC	1 cut(s)	389
BmiI	GGNNCC	1 cut(s)	718
BmrFI	CCNGG	1 cut(s)	11
BmsI	GCATC	1 cut(s)	7
BplI	GAGNNNNNCTC	4 cut(s)	41, 73, 587, 619
Bpu10I	CCTNAGC	1 cut(s)	338
Bpu1102I	GCTNAGC	1 cut(s)	314
BpuEI	CTTGAG	3 cut(s)	43, 293, 344
Bsa29I	ATCGAT	1 cut(s)	144
BsaBI	GATNNNNATC	1 cut(s)	644
BsaJI	CCNNGG	3 cut(s)	10, 163, 247
BsaWI	WCCGGW	1 cut(s)	559
Bse3DI	GCAATG	1 cut(s)	662
Bse8I	GATNNNNATC	1 cut(s)	644
BseBI	CCWGG	1 cut(s)	11
BseCI	ATCGAT	1 cut(s)	144
BseDI	CCNNGG	3 cut(s)	10, 163, 247
BseGI	GGATG	4 cut(s)	333, 500, 531, 613
BseJI	GATNNNNATC	1 cut(s)	644
BseMI	GCAATG	1 cut(s)	662
BseMII	CTCAG	1 cut(s)	710
BseRI	GAGGAG	1 cut(s)	806
BseYI	CCCAGC	1 cut(s)	731
Bsh1236I	CGCG	3 cut(s)	542, 650, 652
BshFI	GGCC	4 cut(s)	390, 419, 473, 800
BshNI	GGYRCC	1 cut(s)	716
BshVI	ATCGAT	1 cut(s)	144
BsiHKAI	GWGCWC	1 cut(s)	264
BsiSI	CCGG	1 cut(s)	560
BsnI	GGCC	4 cut(s)	390, 419, 473, 800
Bsp1286I	GDGCHC	1 cut(s)	264
Bsp143I	GATC	3 cut(s)	505, 568, 580
Bsp1720I	GCTNAGC	1 cut(s)	314
BspANI	GGCC	4 cut(s)	390, 419, 473, 800
BspCNI	CTCAG	1 cut(s)	709
BspDI	ATCGAT	1 cut(s)	144
BspFNI	CGCG	3 cut(s)	542, 650, 652
BspLI	GGNNCC	1 cut(s)	718
BspPI	GGATC	2 cut(s)	500, 563
BspQI	GCTCTTC	2 cut(s)	107, 492
BspT107I	GGYRCC	1 cut(s)	716
BsrDI	GCAATG	1 cut(s)	662
BssECI	CCNNGG	3 cut(s)	10, 163, 247
BssMI	GATC	3 cut(s)	505, 568, 580
BssT1I	CCWWGG	1 cut(s)	247
Bst2UI	CCWGG	1 cut(s)	11
Bst6I	CTCTTC	3 cut(s)	107, 399, 492
BstDEI	CTNAG	5 cut(s)	314, 338, 493, 584, 696
BstF5I	GGATG	4 cut(s)	333, 500, 531, 613
BstFNI	CGCG	3 cut(s)	542, 650, 652
BstHHI	GCGC	2 cut(s)	542, 652
BstKTI	GATC	3 cut(s)	508, 571, 583
BstMBI	GATC	3 cut(s)	505, 568, 580
BstMWI	GCNNNNNNNGC	3 cut(s)	69, 539, 673
BstNI	CCWGG	1 cut(s)	11
BstNSI	RCATGY	2 cut(s)	100, 126
BstSCI	CCNGG	1 cut(s)	9
BstUI	CGCG	3 cut(s)	542, 650, 652
BstXI	CCANNNNNNTGG	2 cut(s)	481, 776
Bsu15I	ATCGAT	1 cut(s)	144
BsuRI	GGCC	4 cut(s)	390, 419, 473, 800
BsuTUI	ATCGAT	1 cut(s)	144
BtgZI	GCGATG	1 cut(s)	631
BtsCI	GGATG	4 cut(s)	333, 500, 531, 613
CfoI	GCGC	2 cut(s)	542, 652
Cfr13I	GGNCC	1 cut(s)	389
ClaI	ATCGAT	1 cut(s)	144
Csp6I	GTAC	1 cut(s)	26
CviAII	CATG	3 cut(s)	97, 123, 784
CviQI	GTAC	1 cut(s)	26
DdeI	CTNAG	5 cut(s)	314, 338, 493, 584, 696
DpnI	GATC	3 cut(s)	507, 570, 582
DpnII	GATC	3 cut(s)	505, 568, 580
EaeI	YGGCCR	1 cut(s)	471
Eam1104I	CTCTTC	3 cut(s)	107, 399, 492
EarI	CTCTTC	3 cut(s)	107, 399, 492
Eco130I	CCWWGG	1 cut(s)	247
EcoRII	CCWGG	1 cut(s)	9
EcoT14I	CCWWGG	1 cut(s)	247
EcoT22I	ATGCAT	1 cut(s)	128
ErhI	CCWWGG	1 cut(s)	247
FaeI	CATG	3 cut(s)	100, 126, 787
FaiI	YATR	9 cut(s)	48, 98, 124, 128, 160, 194, 456, 771, 785
FatI	CATG	3 cut(s)	96, 122, 783
Fnu4HI	GCNGC	1 cut(s)	812
FokI	GGATG	4 cut(s)	320, 487, 538, 600
Fsp4HI	GCNGC	1 cut(s)	812
FspBI	CTAG	3 cut(s)	65, 248, 363
GlaI	GCGC	2 cut(s)	541, 651
GluI	GCNGC	1 cut(s)	812
GsaI	CCCAGC	1 cut(s)	735
HaeIII	GGCC	4 cut(s)	390, 419, 473, 800
HapII	CCGG	1 cut(s)	560
HhaI	GCGC	2 cut(s)	542, 652
Hin1II	CATG	3 cut(s)	100, 126, 787
Hin6I	GCGC	2 cut(s)	540, 650
HinP1I	GCGC	2 cut(s)	540, 650
HincII	GTYRAC	1 cut(s)	94
HindII	GTYRAC	1 cut(s)	94
HindIII	AAGCTT	1 cut(s)	311
HinfI	GANTC	2 cut(s)	229, 692
HpaII	CCGG	1 cut(s)	560
HphI	GGTGA	5 cut(s)	97, 343, 419, 424, 574
Hpy166II	GTNNAC	1 cut(s)	94
Hpy188I	TCNGA	1 cut(s)	505
Hpy188III	TCNNGA	5 cut(s)	118, 269, 278, 509, 698
Hpy8I	GTNNAC	1 cut(s)	94
HpyAV	CCTTC	3 cut(s)	209, 766, 811
HpyCH4V	TGCA	3 cut(s)	126, 172, 533
HpyF10VI	GCNNNNNNNGC	3 cut(s)	69, 539, 673
HpyF3I	CTNAG	5 cut(s)	314, 338, 493, 584, 696
Hsp92II	CATG	3 cut(s)	100, 126, 787
HspAI	GCGC	2 cut(s)	540, 650
Kzo9I	GATC	3 cut(s)	505, 568, 580
LguI	GCTCTTC	2 cut(s)	107, 492
LmnI	GCTCC	1 cut(s)	808
LpnPI	CCDG	9 cut(s)	23, 131, 372, 522, 573, 681, 683, 699, 745
LweI	GCATC	1 cut(s)	7
MaeI	CTAG	3 cut(s)	65, 248, 363
MalI	GATC	3 cut(s)	507, 570, 582
MboI	GATC	3 cut(s)	505, 568, 580
MboII	GAAGA	3 cut(s)	94, 386, 479
MfeI	CAATTG	2 cut(s)	408, 687
MhlI	GDGCHC	1 cut(s)	264
MlsI	TGGCCA	1 cut(s)	473
MluCI	AATT	6 cut(s)	41, 301, 369, 408, 634, 687
MluNI	TGGCCA	1 cut(s)	473
Mox20I	TGGCCA	1 cut(s)	473
Mph1103I	ATGCAT	1 cut(s)	128
MroXI	GAANNNNTTC	1 cut(s)	233
MscI	TGGCCA	1 cut(s)	473
MseI	TTAA	3 cut(s)	633, 705, 817
MslI	CAYNNNNRTG	1 cut(s)	356
Msp20I	TGGCCA	1 cut(s)	473
MspI	CCGG	1 cut(s)	560
MspR9I	CCNGG	1 cut(s)	11
MunI	CAATTG	2 cut(s)	408, 687
MvaI	CCWGG	1 cut(s)	11
MvnI	CGCG	3 cut(s)	542, 650, 652
MwoI	GCNNNNNNNGC	3 cut(s)	69, 539, 673
NdeII	GATC	3 cut(s)	505, 568, 580
NlaIII	CATG	3 cut(s)	100, 126, 787
NlaIV	GGNNCC	1 cut(s)	718
NmeAIII	GCCGAG	1 cut(s)	188
NsiI	ATGCAT	1 cut(s)	128
NspI	RCATGY	2 cut(s)	100, 126
OliI	CACNNNNGTG	1 cut(s)	356
PciSI	GCTCTTC	2 cut(s)	107, 492
PcsI	WCGNNNNNNNCGW	1 cut(s)	276
PdmI	GAANNNNTTC	1 cut(s)	233
PfeI	GAWTC	2 cut(s)	229, 692
PkrI	GCNGC	1 cut(s)	813
PshBI	ATTAAT	2 cut(s)	633, 705
PsiI	TTATAA	1 cut(s)	194
Psp6I	CCWGG	1 cut(s)	9
PspFI	CCCAGC	1 cut(s)	731
PspGI	CCWGG	1 cut(s)	9
PspN4I	GGNNCC	1 cut(s)	718
PspPI	GGNCC	1 cut(s)	389
RsaI	GTAC	1 cut(s)	27
RsaNI	GTAC	1 cut(s)	26
RseI	CAYNNNNRTG	1 cut(s)	356
SapI	GCTCTTC	2 cut(s)	107, 492
SaqAI	TTAA	3 cut(s)	633, 705, 817
SatI	GCNGC	1 cut(s)	812
Sau3AI	GATC	3 cut(s)	505, 568, 580
Sau96I	GGNCC	1 cut(s)	389
ScrFI	CCNGG	1 cut(s)	11
SduI	GDGCHC	1 cut(s)	264
SetI	ASST	9 cut(s)	185, 309, 315, 432, 443, 516, 550, 600, 816
SfaNI	GCATC	1 cut(s)	7
SmiMI	CAYNNNNRTG	1 cut(s)	356
SmlI	CTYRAG	3 cut(s)	58, 308, 323
SmoI	CTYRAG	3 cut(s)	58, 308, 323
Sse9I	AATT	6 cut(s)	41, 301, 369, 408, 634, 687
SspMI	CTAG	3 cut(s)	65, 248, 363
StyD4I	CCNGG	1 cut(s)	9
StyI	CCWWGG	1 cut(s)	247
TaqI	TCGA	6 cut(s)	144, 270, 279, 462, 638, 804
TasI	AATT	6 cut(s)	41, 301, 369, 408, 634, 687
TfiI	GAWTC	2 cut(s)	229, 692
Tru1I	TTAA	3 cut(s)	633, 705, 817
Tru9I	TTAA	3 cut(s)	633, 705, 817
TseI	GCWGC	1 cut(s)	811
TspDTI	ATGAA	2 cut(s)	602, 639
VspI	ATTAAT	2 cut(s)	633, 705
XceI	RCATGY	2 cut(s)	100, 126
XmaJI	CCTAGG	1 cut(s)	247
XmnI	GAANNNNTTC	1 cut(s)	233
XspI	CTAG	3 cut(s)	65, 248, 363
Zsp2I	ATGCAT	1 cut(s)	128

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.