FvH4_6g08120

Pyroglutamyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005844
Physical Location & Seq
Forward (+)
4826436 .. 4829500
3065 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g08120.t1

Sequence Viewer

Length: 654 bp
ATGGGTTCCGAAGGTCCTGCAACAATAACAATTCATGTGACGGGTTTTAAGAAATTTCATGGAGTTTCTGAGAATCCAACTGAGACAATTGTCAGTAATCTCACCGATTATGTGAAGAAGAATGGTTCACCAAAAGGTCTGGTTATTGGTAGTTGCAGCATTCTTGAAACTGCAGGACAAGGAGCAGTTCTTCCCCTATACCAGACATTGCAATCTGCAATAAGCGAGAAGGACGCTACAAGTTCCAGCAGAATTATTTGGGTACACTTTGGAGTTAATAGTGGTGCAACTAAGTTTGCTATTGAGCATCAAGCTGTCAATGAAGCTACTTTCCGTTGCCCTGATGAGATGGGATGGAAGCCTCAGAAAGTCCCTATCATTCCTTCAGATGGTGGAATTTCACATATAAGAGAGACTTCTCTTCCAGTTGAGGAGATAACCAAGGCCTTGGCAAAGAAGGGGTTTGAGGTGATGACCTCAGATGATGCAGGGCGCTTTGTATGCAACTATGTCTACTATCATTCCCTTCGGTTCTCAGAGCAAAATGGGATCAAATCACTCTTTGTGCACGTGCCTCTCTTCGTGACTATAGACGAGGACACCCAAATGAAATTTGCTGCTTCCTTGCTAGAGGTACTTACATCATTATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C15

Protein Analysis

218

Amino Acids

23.63

Weight (kDa)

5.97

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C15 PF01470 11 - 194 1.5e-18 Pyroglutamyl peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 513
AclWI GGATC 1 cut(s) 557
AcsI RAATTY 3 cut(s) 53, 396, 611
AcuI CTGAAG 1 cut(s) 369
AcvI CACGTG 1 cut(s) 571
AfaI GTAC 2 cut(s) 264, 636
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 1 cut(s) 167
AluBI AGCT 2 cut(s) 314, 326
AluI AGCT 2 cut(s) 314, 326
Alw21I GWGCWC 1 cut(s) 570
Alw26I GTCTC 2 cut(s) 77, 407
Alw44I GTGCAC 1 cut(s) 566
AlwI GGATC 1 cut(s) 557
AoxI GGCC 1 cut(s) 444
ApaLI GTGCAC 1 cut(s) 566
ApeKI GCWGC 2 cut(s) 156, 617
ApoI RAATTY 3 cut(s) 53, 396, 611
AspLEI GCGC 1 cut(s) 495
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 3 cut(s) 94, 120, 481
AvaII GGWCC 1 cut(s) 14
BaeGI GKGCMC 1 cut(s) 570
BbrPI CACGTG 1 cut(s) 571
Bbv12I GWGCWC 1 cut(s) 570
BbvI GCAGC 2 cut(s) 168, 604
BccI CCATC 3 cut(s) 343, 348, 383
BcgI CGANNNNNNTGC 1 cut(s) 33
BcoDI GTCTC 2 cut(s) 77, 407
BfaI CTAG 2 cut(s) 629, 652
BfmI CTRYAG 2 cut(s) 171, 588
BfoI RGCGCY 1 cut(s) 496
BisI GCNGC 2 cut(s) 157, 618
BlsI GCNGC 2 cut(s) 158, 619
Bme18I GGWCC 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 14
BmiI GGNNCC 1 cut(s) 7
BmsI GCATC 2 cut(s) 316, 475
BoxI GACNNNNGTC 1 cut(s) 89
BsaAI YACGTR 1 cut(s) 571
BsaJI CCNNGG 2 cut(s) 441, 447
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 206
BseDI CCNNGG 2 cut(s) 441, 447
BseGI GGATG 1 cut(s) 359
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 5 cut(s) 60, 72, 377, 492, 549
BseNI ACTGG 1 cut(s) 425
BseRI GAGGAG 1 cut(s) 446
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 2 cut(s) 168, 604
BshFI GGCC 1 cut(s) 446
BsiHKAI GWGCWC 1 cut(s) 570
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 2 cut(s) 77, 407
BsmFI GGGAC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 159
BsnI GGCC 1 cut(s) 446
Bsp1286I GDGCHC 1 cut(s) 570
Bsp143I GATC 1 cut(s) 549
BspANI GGCC 1 cut(s) 446
BspCNI CTCAG 5 cut(s) 61, 73, 376, 491, 548
BspLI GGNNCC 1 cut(s) 7
BspMAI CTGCAG 1 cut(s) 175
BspPI GGATC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 206
BsrI ACTGG 1 cut(s) 425
BssECI CCNNGG 2 cut(s) 441, 447
BssMI GATC 1 cut(s) 549
BssT1I CCWWGG 2 cut(s) 441, 447
Bst6I CTCTTC 2 cut(s) 426, 584
BstBAI YACGTR 1 cut(s) 571
BstDEI CTNAG 6 cut(s) 69, 81, 291, 363, 478, 535
BstF5I GGATG 1 cut(s) 359
BstH2I RGCGCY 1 cut(s) 496
BstHHI GCGC 1 cut(s) 495
BstKTI GATC 1 cut(s) 552
BstMAI GTCTC 2 cut(s) 77, 407
BstMBI GATC 1 cut(s) 549
BstMWI GCNNNNNNNGC 1 cut(s) 501
BstPAI GACNNNNGTC 1 cut(s) 89
BstSFI CTRYAG 2 cut(s) 171, 588
BstSLI GKGCMC 1 cut(s) 570
BstV1I GCAGC 2 cut(s) 168, 604
BstXI CCANNNNNNTGG 1 cut(s) 448
BsuRI GGCC 1 cut(s) 446
BtsCI GGATG 1 cut(s) 359
CfoI GCGC 1 cut(s) 495
Cfr13I GGNCC 1 cut(s) 14
CseI GACGC 1 cut(s) 242
Csp6I GTAC 2 cut(s) 263, 635
CviAII CATG 2 cut(s) 35, 59
CviJI RGCY 4 cut(s) 314, 326, 361, 446
CviKI_1 RGCY 4 cut(s) 314, 326, 361, 446
CviQI GTAC 2 cut(s) 263, 635
DdeI CTNAG 6 cut(s) 69, 81, 291, 363, 478, 535
DpnI GATC 1 cut(s) 551
DpnII GATC 1 cut(s) 549
Eam1104I CTCTTC 2 cut(s) 426, 584
EarI CTCTTC 2 cut(s) 426, 584
Eco130I CCWWGG 2 cut(s) 441, 447
Eco147I AGGCCT 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 14
Eco57I CTGAAG 1 cut(s) 369
Eco72I CACGTG 1 cut(s) 571
EcoO109I RGGNCCY 1 cut(s) 14
EcoT14I CCWWGG 2 cut(s) 441, 447
ErhI CCWWGG 2 cut(s) 441, 447
FaeI CATG 2 cut(s) 38, 62
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FaqI GGGAC 1 cut(s) 356
FatI CATG 2 cut(s) 34, 58
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 157, 618
FokI GGATG 1 cut(s) 366
Fsp4HI GCNGC 2 cut(s) 157, 618
FspBI CTAG 2 cut(s) 629, 652
GlaI GCGC 1 cut(s) 494
GluI GCNGC 2 cut(s) 157, 618
HaeII RGCGCY 1 cut(s) 496
HaeIII GGCC 1 cut(s) 446
HgaI GACGC 1 cut(s) 242
HhaI GCGC 1 cut(s) 495
Hin1II CATG 2 cut(s) 38, 62
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HinfI GANTC 1 cut(s) 73
HphI GGTGA 3 cut(s) 94, 120, 481
Hpy166II GTNNAC 4 cut(s) 128, 265, 514, 568
Hpy188I TCNGA 6 cut(s) 10, 70, 366, 388, 481, 538
Hpy188III TCNNGA 2 cut(s) 164, 583
Hpy8I GTNNAC 4 cut(s) 128, 265, 514, 568
HpyAV CCTTC 5 cut(s) 5, 223, 393, 451, 536
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 9 cut(s) 20, 156, 173, 211, 218, 287, 488, 504, 568
HpyF10VI GCNNNNNNNGC 1 cut(s) 501
HpyF3I CTNAG 6 cut(s) 69, 81, 291, 363, 478, 535
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 2 cut(s) 38, 62
HspAI GCGC 1 cut(s) 493
Kzo9I GATC 1 cut(s) 549
LmnI GCTCC 1 cut(s) 182
LpnPI CCDG 8 cut(s) 30, 125, 159, 215, 259, 354, 438, 474
Lsp1109I GCAGC 2 cut(s) 168, 604
LweI GCATC 2 cut(s) 316, 475
MaeI CTAG 2 cut(s) 629, 652
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 2 cut(s) 37, 583
MalI GATC 1 cut(s) 551
MboI GATC 1 cut(s) 549
MboII GAAGA 5 cut(s) 127, 130, 182, 413, 571
MfeI CAATTG 1 cut(s) 87
MhlI GDGCHC 1 cut(s) 570
MluCI AATT 6 cut(s) 30, 53, 87, 252, 396, 611
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 7 cut(s) 372, 424, 460, 487, 585, 589, 625
MseI TTAA 2 cut(s) 48, 276
MslI CAYNNNNRTG 2 cut(s) 605, 646
MunI CAATTG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 501
NdeII GATC 1 cut(s) 549
NlaIII CATG 2 cut(s) 38, 62
NlaIV GGNNCC 1 cut(s) 7
NmuCI GTSAC 2 cut(s) 37, 583
PceI AGGCCT 1 cut(s) 446
PctI GAATGC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 73
PkrI GCNGC 2 cut(s) 158, 619
PmaCI CACGTG 1 cut(s) 571
PmlI CACGTG 1 cut(s) 571
Ppu21I YACGTR 1 cut(s) 571
PpuMI RGGWCCY 1 cut(s) 14
PshAI GACNNNNGTC 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 14
PspCI CACGTG 1 cut(s) 571
PspN4I GGNNCC 1 cut(s) 7
PspPI GGNCC 1 cut(s) 14
PspPPI RGGWCCY 1 cut(s) 14
PstI CTGCAG 1 cut(s) 175
RsaI GTAC 2 cut(s) 264, 636
RsaNI GTAC 2 cut(s) 263, 635
RseI CAYNNNNRTG 2 cut(s) 605, 646
SaqAI TTAA 2 cut(s) 48, 276
SatI GCNGC 2 cut(s) 157, 618
Sau3AI GATC 1 cut(s) 549
Sau96I GGNCC 1 cut(s) 14
SduI GDGCHC 1 cut(s) 570
SetI ASST 8 cut(s) 16, 139, 316, 328, 471, 479, 573, 636
SfaNI GCATC 2 cut(s) 316, 475
SfcI CTRYAG 2 cut(s) 171, 588
SinI GGWCC 1 cut(s) 14
SmiMI CAYNNNNRTG 2 cut(s) 605, 646
Sse9I AATT 6 cut(s) 30, 53, 87, 252, 396, 611
SseBI AGGCCT 1 cut(s) 446
SspMI CTAG 2 cut(s) 629, 652
StuI AGGCCT 1 cut(s) 446
StyI CCWWGG 2 cut(s) 441, 447
TaiI ACGT 1 cut(s) 573
TasI AATT 6 cut(s) 30, 53, 87, 252, 396, 611
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 2 cut(s) 48, 276
Tru9I TTAA 2 cut(s) 48, 276
TseFI GTSAC 2 cut(s) 37, 583
TseI GCWGC 2 cut(s) 156, 617
Tsp45I GTSAC 2 cut(s) 37, 583
TspDTI ATGAA 4 cut(s) 23, 47, 336, 623
TspGWI ACGGA 1 cut(s) 323
VneI GTGCAC 1 cut(s) 566
VpaK11BI GGWCC 1 cut(s) 14
XapI RAATTY 3 cut(s) 53, 396, 611
XmiI GTMKAC 1 cut(s) 513
XspI CTAG 2 cut(s) 629, 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.