MD04G1189700.v1.1

Pyroglutamyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005844
Physical Location & Seq
Reverse (-)
27991895 .. 27996382
4488 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1189700.v1.1.491

Sequence Viewer

Length: 672 bp
ATGGGGTCTGAAGGGCCTTCGCCAGTAACTATTCATGTGACCGGTTTTAAGAAATTCCACGGAGTTTCGGAGAATCCGACTGAGACAATTGTCAGTAATCTCACGGAGTATGTGAAGAAGGAGGGTTCACCAAAAGGTCTGGTTCTTGGAAGTTGCAGCATTCTTGAGACTGCAGGACAAGGAGCACTTGTCGCCCTGTACCAGACATTGCGATCTGCTATCAGCGAGCAGGATACTTCAAATTCCAGCAGAACTATTTGGTTACACTTTGGTGTTAATAGTGGTGCAACAAAGTTTGCTCTCGAGCACCAAGCGGTCAATGAAGCTACTTTCCGTTGCCCTGATGAAATGGGATGGAAGCCTCAGAAAGTTCCGATTATTCCTTCAGATGGTGGAATTGCACATATACGAGAGACTTCTCTTCCCGTTGAGGAAATAACAAAGGCCTTGGCAAAGAAGGGTTATGAGGTGATGACCTCGGATGATGCAGGTCGGTTTGTTTGCAATTACGTCTACTACCATTCCCTTCGGTTTGCAGAGCAAAACGGGATCAAGTCACTCTTTGTTCATGTGCCTCTCTTCTTGACCATAGACGAGGAGACCCAAATGCAGTTTGCTGCTTCCTTGCTAGAGAAAACTGGTGGAATTGTAAGGCTCTGCACTGTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C15

Protein Analysis

224

Amino Acids

24.32

Weight (kDa)

5.98

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C15 PF01470 11 - 194 7.9e-18 Pyroglutamyl peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 479
AccI GTMKAC 1 cut(s) 513
AciI CCGC 1 cut(s) 314
AclWI GGATC 1 cut(s) 557
AcsI RAATTY 2 cut(s) 53, 241
AcuI CTGAAG 2 cut(s) 30, 369
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 1 cut(s) 389
AgeI ACCGGT 1 cut(s) 41
AgsI TTSAA 1 cut(s) 240
AleI CACNNNNGTG 1 cut(s) 270
AluBI AGCT 1 cut(s) 326
AluI AGCT 1 cut(s) 326
Alw21I GWGCWC 2 cut(s) 187, 309
Alw26I GTCTC 4 cut(s) 77, 161, 407, 593
AlwI GGATC 1 cut(s) 557
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 2 cut(s) 14, 444
ApeKI GCWGC 2 cut(s) 156, 617
ApoI RAATTY 2 cut(s) 53, 241
AsiGI ACCGGT 1 cut(s) 41
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 2 cut(s) 120, 481
AvaI CYCGRG 1 cut(s) 302
Bbv12I GWGCWC 2 cut(s) 187, 309
BbvI GCAGC 2 cut(s) 168, 604
BccI CCATC 2 cut(s) 348, 383
BciVI GTATCC 1 cut(s) 226
BcoDI GTCTC 4 cut(s) 77, 161, 407, 593
BfaI CTAG 1 cut(s) 629
BfmI CTRYAG 1 cut(s) 171
BfuAI ACCTGC 1 cut(s) 479
BfuI GTATCC 1 cut(s) 226
BisI GCNGC 2 cut(s) 157, 618
BlsI GCNGC 2 cut(s) 158, 619
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 14
BmsI GCATC 1 cut(s) 475
BoxI GACNNNNGTC 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 185
BsaI GGTCTC 1 cut(s) 593
BsaJI CCNNGG 3 cut(s) 58, 447, 477
BsaWI WCCGGW 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 2 cut(s) 23, 643
Bse3DI GCAATG 1 cut(s) 206
BseDI CCNNGG 3 cut(s) 58, 447, 477
BseGI GGATG 2 cut(s) 359, 487
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 2 cut(s) 72, 377
BseNI ACTGG 2 cut(s) 23, 643
BseRI GAGGAG 1 cut(s) 611
BseXI GCAGC 2 cut(s) 168, 604
BsgI GTGCAG 1 cut(s) 643
BshFI GGCC 2 cut(s) 16, 446
BshTI ACCGGT 1 cut(s) 41
BsiHKAI GWGCWC 2 cut(s) 187, 309
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 1 cut(s) 42
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 4 cut(s) 77, 161, 407, 593
BsmI GAATGC 1 cut(s) 159
BsnI GGCC 2 cut(s) 16, 446
Bso31I GGTCTC 1 cut(s) 593
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 2 cut(s) 187, 309
Bsp143I GATC 2 cut(s) 212, 549
BspACI CCGC 1 cut(s) 314
BspANI GGCC 2 cut(s) 16, 446
BspCNI CTCAG 2 cut(s) 73, 376
BspMAI CTGCAG 1 cut(s) 175
BspMI ACCTGC 1 cut(s) 479
BspPI GGATC 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 593
BsrDI GCAATG 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 2 cut(s) 23, 643
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 3 cut(s) 58, 447, 477
BssMI GATC 2 cut(s) 212, 549
BssT1I CCWWGG 1 cut(s) 447
Bst4CI ACNGT 1 cut(s) 664
Bst6I CTCTTC 2 cut(s) 426, 584
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 2 cut(s) 81, 363
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 2 cut(s) 359, 487
BstKTI GATC 2 cut(s) 215, 552
BstMAI GTCTC 4 cut(s) 77, 161, 407, 593
BstMBI GATC 2 cut(s) 212, 549
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstPAI GACNNNNGTC 1 cut(s) 89
BstSFI CTRYAG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 168, 604
BsuI GTATCC 1 cut(s) 226
BsuRI GGCC 2 cut(s) 16, 446
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 2 cut(s) 359, 487
BtsIMutI CAGTG 1 cut(s) 660
BveI ACCTGC 1 cut(s) 479
Cac8I GCNNGC 1 cut(s) 227
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 199
CspAI ACCGGT 1 cut(s) 41
CviAII CATG 2 cut(s) 35, 569
CviJI RGCY 5 cut(s) 16, 326, 361, 446, 655
CviKI_1 RGCY 5 cut(s) 16, 326, 361, 446, 655
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 2 cut(s) 81, 363
DpnI GATC 2 cut(s) 214, 551
DpnII GATC 2 cut(s) 212, 549
Eam1104I CTCTTC 2 cut(s) 426, 584
EarI CTCTTC 2 cut(s) 426, 584
Eco130I CCWWGG 1 cut(s) 447
Eco147I AGGCCT 1 cut(s) 446
Eco31I GGTCTC 1 cut(s) 593
Eco57I CTGAAG 2 cut(s) 30, 369
Eco88I CYCGRG 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 14
EcoT14I CCWWGG 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 2 cut(s) 38, 572
FaiI YATR 8 cut(s) 36, 111, 405, 407, 465, 570, 590, 670
FalI AAGNNNNNCTT 4 cut(s) 171, 203, 545, 577
FatI CATG 2 cut(s) 34, 568
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 157, 618
FokI GGATG 2 cut(s) 366, 494
Fsp4HI GCNGC 2 cut(s) 157, 618
FspBI CTAG 1 cut(s) 629
GluI GCNGC 2 cut(s) 157, 618
HaeIII GGCC 2 cut(s) 16, 446
HapII CCGG 1 cut(s) 42
Hin1II CATG 2 cut(s) 38, 572
HinfI GANTC 1 cut(s) 73
HpaII CCGG 1 cut(s) 42
HphI GGTGA 2 cut(s) 120, 481
Hpy166II GTNNAC 2 cut(s) 128, 514
Hpy188I TCNGA 7 cut(s) 10, 70, 78, 366, 375, 388, 481
Hpy188III TCNNGA 3 cut(s) 164, 302, 583
Hpy8I GTNNAC 2 cut(s) 128, 514
HpyAV CCTTC 6 cut(s) 5, 27, 112, 393, 451, 536
HpyCH4III ACNGT 1 cut(s) 664
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 9 cut(s) 156, 173, 287, 401, 488, 504, 536, 610, 660
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpyF3I CTNAG 2 cut(s) 81, 363
HpySE526I ACGT 1 cut(s) 510
Hsp92II CATG 2 cut(s) 38, 572
Kzo9I GATC 2 cut(s) 212, 549
LmnI GCTCC 1 cut(s) 182
Lsp1109I GCAGC 2 cut(s) 168, 604
LweI GCATC 1 cut(s) 475
MaeI CTAG 1 cut(s) 629
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 4 cut(s) 25, 37, 261, 555
MalI GATC 2 cut(s) 214, 551
MboI GATC 2 cut(s) 212, 549
MboII GAAGA 3 cut(s) 127, 413, 571
MfeI CAATTG 1 cut(s) 87
MhlI GDGCHC 2 cut(s) 187, 309
MluCI AATT 6 cut(s) 53, 87, 241, 396, 505, 645
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 7 cut(s) 115, 372, 424, 460, 487, 585, 589
MseI TTAA 2 cut(s) 48, 276
MslI CAYNNNNRTG 1 cut(s) 270
MspI CCGG 1 cut(s) 42
MunI CAATTG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 191
NdeII GATC 2 cut(s) 212, 549
NlaIII CATG 2 cut(s) 38, 572
NmuCI GTSAC 2 cut(s) 37, 555
OliI CACNNNNGTG 1 cut(s) 270
PaeR7I CTCGAG 1 cut(s) 302
PceI AGGCCT 1 cut(s) 446
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PctI GAATGC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 73
PinAI ACCGGT 1 cut(s) 41
PkrI GCNGC 2 cut(s) 158, 619
PshAI GACNNNNGTC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 14
PstI CTGCAG 1 cut(s) 175
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 270
SaqAI TTAA 2 cut(s) 48, 276
SatI GCNGC 2 cut(s) 157, 618
Sau3AI GATC 2 cut(s) 212, 549
Sau96I GGNCC 1 cut(s) 14
SduI GDGCHC 2 cut(s) 187, 309
SetI ASST 6 cut(s) 139, 328, 471, 479, 493, 513
SfaNI GCATC 1 cut(s) 475
SfcI CTRYAG 1 cut(s) 171
Sfr274I CTCGAG 1 cut(s) 302
SlaI CTCGAG 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 2 cut(s) 164, 302
SmoI CTYRAG 2 cut(s) 164, 302
Sse9I AATT 6 cut(s) 53, 87, 241, 396, 505, 645
SseBI AGGCCT 1 cut(s) 446
SsiI CCGC 1 cut(s) 314
SspMI CTAG 1 cut(s) 629
StuI AGGCCT 1 cut(s) 446
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 1 cut(s) 664
TaiI ACGT 1 cut(s) 513
TaqI TCGA 1 cut(s) 303
TasI AATT 6 cut(s) 53, 87, 241, 396, 505, 645
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 2 cut(s) 48, 276
Tru9I TTAA 2 cut(s) 48, 276
TscAI CASTG 1 cut(s) 667
TseFI GTSAC 2 cut(s) 37, 555
TseI GCWGC 2 cut(s) 156, 617
Tsp45I GTSAC 2 cut(s) 37, 555
TspDTI ATGAA 4 cut(s) 23, 336, 360, 557
TspGWI ACGGA 3 cut(s) 75, 119, 323
TspRI CASTG 1 cut(s) 667
XapI RAATTY 2 cut(s) 53, 241
XhoI CTCGAG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 513
XspI CTAG 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.