Prupe.6G307500_v2.0.a1

Pyroglutamyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0005844
Physical Location & Seq
Reverse (-)
27705008 .. 27708090
3083 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G307500.2

Sequence Viewer

Length: 654 bp
ATGGGGTCTGAAGGACCTTCAACAGTAACAATTCATGTGACTGGTTTTAAGAAATTCCATGGAGTTTCAGAGAATCCAACTGAGACTATTGTCAGTAATCTCAAAGAGTATGTGAAGAAGAAGGGTTCACCAAAGGGTCTGGTTCTTGGAAGTTGCAGCATTCTTGAGACTGCAGGACAAGGAGCAGTTGTTCCCCTATACCAGACATTGCGATCTGCTATCAGCGAGCAGGATTCTTCAAATTCCAGTAGAATTATTTGGGTACACTTTGGTGTTAATAGTGGTGCAACAAGGTTTGCTATTGAGCATCAAGCTGTCAATGAAGCTACTTTTCGTTGCCCTGATGAGATGGGATGGAAGCCTCAGAAAGTCCCCATTATTCCTTCAGATGGTGGAATTTCACATATACGAGAGACTTCTCTTCCTGTTGAGGACATAACAAAGGCCTTGGCAAAGAAGGGTTTTGAGGTGATGACCTCAGACGATGCAGGCCGCTTTGTATGCAACTATGTCTACTACCATTCCCTTCGGTTTGCAGAGCAAAACGGGATCAAATCGCTCTTTGTTCATGTGCCTCTGTTCTTGACCATAGATGAGGACACACAAATGCAATTTGCTGCTTCCTTGTTAGAGGTGCTCACTTCATTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C15

Protein Analysis

218

Amino Acids

23.75

Weight (kDa)

6.16

Isoelectric Point (pI)

33.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 513
AciI CCGC 1 cut(s) 493
AclWI GGATC 1 cut(s) 557
AcsI RAATTY 3 cut(s) 53, 241, 396
AcuI CTGAAG 2 cut(s) 30, 369
AfaI GTAC 1 cut(s) 264
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 2 cut(s) 21, 240
AleI CACNNNNGTG 1 cut(s) 270
AloI GAACNNNNNNTCC 2 cut(s) 174, 206
AluBI AGCT 2 cut(s) 314, 326
AluI AGCT 2 cut(s) 314, 326
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 3 cut(s) 77, 161, 407
AlwI GGATC 1 cut(s) 557
AoxI GGCC 2 cut(s) 444, 490
ApeKI GCWGC 2 cut(s) 156, 617
ApoI RAATTY 3 cut(s) 53, 241, 396
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 2 cut(s) 120, 481
AvaII GGWCC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 2 cut(s) 168, 604
BccI CCATC 3 cut(s) 343, 348, 383
BcoDI GTCTC 3 cut(s) 77, 161, 407
BfmI CTRYAG 1 cut(s) 171
BisI GCNGC 3 cut(s) 157, 493, 618
BlsI GCNGC 3 cut(s) 158, 494, 619
Bme18I GGWCC 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 14
BmsI GCATC 2 cut(s) 316, 475
BoxI GACNNNNGTC 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 185
BsaJI CCNNGG 2 cut(s) 58, 447
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 2 cut(s) 46, 246
Bse3DI GCAATG 1 cut(s) 206
BseDI CCNNGG 2 cut(s) 58, 447
BseGI GGATG 1 cut(s) 359
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 3 cut(s) 72, 377, 492
BseNI ACTGG 2 cut(s) 46, 246
BseXI GCAGC 2 cut(s) 168, 604
BshFI GGCC 2 cut(s) 446, 492
BsiHKAI GWGCWC 1 cut(s) 639
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 3 cut(s) 77, 161, 407
BsmFI GGGAC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 159
BsnI GGCC 2 cut(s) 446, 492
Bsp1286I GDGCHC 1 cut(s) 639
Bsp143I GATC 2 cut(s) 212, 549
Bsp19I CCATGG 1 cut(s) 58
BspACI CCGC 1 cut(s) 493
BspANI GGCC 2 cut(s) 446, 492
BspCNI CTCAG 3 cut(s) 73, 376, 491
BspMAI CTGCAG 1 cut(s) 175
BspPI GGATC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 206
BsrI ACTGG 2 cut(s) 46, 246
BssECI CCNNGG 2 cut(s) 58, 447
BssMI GATC 2 cut(s) 212, 549
BssT1I CCWWGG 2 cut(s) 58, 447
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 426
BstC8I GCNNGC 2 cut(s) 227, 490
BstDEI CTNAG 3 cut(s) 81, 363, 478
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 1 cut(s) 359
BstKTI GATC 2 cut(s) 215, 552
BstMAI GTCTC 3 cut(s) 77, 161, 407
BstMBI GATC 2 cut(s) 212, 549
BstMWI GCNNNNNNNGC 1 cut(s) 501
BstPAI GACNNNNGTC 1 cut(s) 89
BstSFI CTRYAG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 168, 604
BsuRI GGCC 2 cut(s) 446, 492
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 1 cut(s) 359
Cac8I GCNNGC 2 cut(s) 227, 490
Cfr13I GGNCC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 263
CviAII CATG 3 cut(s) 35, 59, 569
CviJI RGCY 5 cut(s) 314, 326, 361, 446, 492
CviKI_1 RGCY 5 cut(s) 314, 326, 361, 446, 492
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 3 cut(s) 81, 363, 478
DpnI GATC 2 cut(s) 214, 551
DpnII GATC 2 cut(s) 212, 549
Eam1104I CTCTTC 1 cut(s) 426
EarI CTCTTC 1 cut(s) 426
Eco130I CCWWGG 2 cut(s) 58, 447
Eco147I AGGCCT 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 14
Eco57I CTGAAG 2 cut(s) 30, 369
EcoO109I RGGNCCY 1 cut(s) 14
EcoT14I CCWWGG 2 cut(s) 58, 447
ErhI CCWWGG 2 cut(s) 58, 447
FaeI CATG 3 cut(s) 38, 62, 572
FaqI GGGAC 1 cut(s) 356
FatI CATG 3 cut(s) 34, 58, 568
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 3 cut(s) 157, 493, 618
FokI GGATG 1 cut(s) 366
Fsp4HI GCNGC 3 cut(s) 157, 493, 618
GluI GCNGC 3 cut(s) 157, 493, 618
HaeIII GGCC 2 cut(s) 446, 492
Hin1II CATG 3 cut(s) 38, 62, 572
HinfI GANTC 2 cut(s) 73, 233
HphI GGTGA 2 cut(s) 120, 481
Hpy166II GTNNAC 3 cut(s) 128, 265, 514
Hpy188I TCNGA 5 cut(s) 10, 70, 366, 388, 481
Hpy188III TCNNGA 2 cut(s) 164, 583
Hpy8I GTNNAC 3 cut(s) 128, 265, 514
HpyAV CCTTC 6 cut(s) 5, 27, 115, 393, 451, 536
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4V TGCA 7 cut(s) 156, 173, 287, 488, 504, 536, 610
HpyF10VI GCNNNNNNNGC 1 cut(s) 501
HpyF3I CTNAG 3 cut(s) 81, 363, 478
Hsp92II CATG 3 cut(s) 38, 62, 572
Kzo9I GATC 2 cut(s) 212, 549
LmnI GCTCC 1 cut(s) 182
LpnPI CCDG 9 cut(s) 27, 125, 159, 215, 215, 259, 354, 438, 474
Lsp1109I GCAGC 2 cut(s) 168, 604
LweI GCATC 2 cut(s) 316, 475
MaeIII GTNAC 2 cut(s) 25, 37
MalI GATC 2 cut(s) 214, 551
MboI GATC 2 cut(s) 212, 549
MboII GAAGA 4 cut(s) 127, 130, 228, 413
MhlI GDGCHC 1 cut(s) 639
MluCI AATT 7 cut(s) 30, 53, 241, 252, 396, 611, 649
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 7 cut(s) 372, 424, 460, 487, 585, 589, 625
MseI TTAA 3 cut(s) 48, 276, 647
MslI CAYNNNNRTG 2 cut(s) 270, 605
Mva1269I GAATGC 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 501
NcoI CCATGG 1 cut(s) 58
NdeII GATC 2 cut(s) 212, 549
NlaIII CATG 3 cut(s) 38, 62, 572
NmuCI GTSAC 1 cut(s) 37
OliI CACNNNNGTG 1 cut(s) 270
PceI AGGCCT 1 cut(s) 446
PctI GAATGC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 73, 233
PkrI GCNGC 3 cut(s) 158, 494, 619
PpuMI RGGWCCY 1 cut(s) 14
PshAI GACNNNNGTC 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 14
PspPI GGNCC 1 cut(s) 14
PspPPI RGGWCCY 1 cut(s) 14
PstI CTGCAG 1 cut(s) 175
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
RseI CAYNNNNRTG 2 cut(s) 270, 605
SaqAI TTAA 3 cut(s) 48, 276, 647
SatI GCNGC 3 cut(s) 157, 493, 618
Sau3AI GATC 2 cut(s) 212, 549
Sau96I GGNCC 1 cut(s) 14
SduI GDGCHC 1 cut(s) 639
SetI ASST 7 cut(s) 19, 296, 316, 328, 471, 479, 636
SfaNI GCATC 2 cut(s) 316, 475
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 1 cut(s) 14
SmiMI CAYNNNNRTG 2 cut(s) 270, 605
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 7 cut(s) 30, 53, 241, 252, 396, 611, 649
SseBI AGGCCT 1 cut(s) 446
SsiI CCGC 1 cut(s) 493
StuI AGGCCT 1 cut(s) 446
StyI CCWWGG 2 cut(s) 58, 447
TaaI ACNGT 1 cut(s) 25
TasI AATT 7 cut(s) 30, 53, 241, 252, 396, 611, 649
TauI GCSGC 1 cut(s) 495
TfiI GAWTC 2 cut(s) 73, 233
Tru1I TTAA 3 cut(s) 48, 276, 647
Tru9I TTAA 3 cut(s) 48, 276, 647
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 2 cut(s) 156, 617
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 4 cut(s) 23, 336, 557, 633
VpaK11BI GGWCC 1 cut(s) 14
XapI RAATTY 3 cut(s) 53, 241, 396
XmiI GTMKAC 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.