Rroxscaffold_6G00421420

Pyroglutamyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005844
Physical Location & Seq
Reverse (-)
42581402 .. 42584146
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00421420.1

Sequence Viewer

Length: 654 bp
ATGGGTTCCGAAGGTCCTGCAACAATAACAATTCATGTGACCGGTTTTAAGAAATTCCATGGAGTTTCAGAGAATCCAACTGAGACAATTGTCAGTAATCTCACTGATTATGTGAAAAAGAATGGTTCACCAAAAGGTCTTGTTATTGGAAGTTGCAGCATTCTTGAGACTGCAGGACAAGGAGCACTTCTTCCCCTATACCAGACATTGCGATCTGCAATAAGCGAGAAGGACTCTACAAATTCCAGCAGAATTATTTGGGTACACTTTGGAGTTAATAGTGGTGCAACTAAGTTTGCCATTGAGCATCAAGCTGTCAATGAAGCTACTTTTCGTTGCCCTGATGAGATGGGATGGAAGCCTCAGAAAGTCCCTATCATTCCTTCAGATGGTGGAATTTCATATATAAGAGAGACTTCTCTGCCAGTTGAGGAGATAACCAAGGCCTTGGCAAAGAAGGGTTATGAGGTGATGACCTCGGATGATGCAGGGCGCTTTGTATGCAATTATGTCTACTATCATTCCCTTCGGTTTGCAGAGCAAAATGGGATCAAATCGCTCTTTGTGCATGTGCCTCTCTTCTTGACTATAGATGAGGACACCCAAATGAAATTTGCTGCATCCTTGCTAGAGGTACTTACATCATTATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C15

Protein Analysis

217

Amino Acids

23.75

Weight (kDa)

6.07

Isoelectric Point (pI)

29.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C15 PF01470 11 - 194 1.5e-18 Pyroglutamyl peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 513
AclWI GGATC 1 cut(s) 557
AcsI RAATTY 4 cut(s) 53, 241, 396, 611
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 2 cut(s) 264, 636
AfiI CCNNNNNNNGG 1 cut(s) 389
AgeI ACCGGT 1 cut(s) 41
AluBI AGCT 2 cut(s) 314, 326
AluI AGCT 2 cut(s) 314, 326
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 3 cut(s) 77, 161, 407
AlwI GGATC 1 cut(s) 557
AoxI GGCC 1 cut(s) 444
ApeKI GCWGC 2 cut(s) 156, 617
ApoI RAATTY 4 cut(s) 53, 241, 396, 611
AsiGI ACCGGT 1 cut(s) 41
AspLEI GCGC 1 cut(s) 495
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 2 cut(s) 120, 481
AvaII GGWCC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 187
BbvI GCAGC 2 cut(s) 168, 604
BccI CCATC 3 cut(s) 343, 348, 383
BcgI CGANNNNNNTGC 1 cut(s) 33
BcoDI GTCTC 3 cut(s) 77, 161, 407
BfaI CTAG 1 cut(s) 629
BfmI CTRYAG 2 cut(s) 171, 588
BfoI RGCGCY 1 cut(s) 496
BisI GCNGC 2 cut(s) 157, 618
BlsI GCNGC 2 cut(s) 158, 619
Bme18I GGWCC 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 14
BmiI GGNNCC 1 cut(s) 7
BmsI GCATC 3 cut(s) 316, 475, 629
BoxI GACNNNNGTC 1 cut(s) 89
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpuEI CTTGAG 1 cut(s) 185
BsaJI CCNNGG 4 cut(s) 58, 441, 447, 477
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 206
BseDI CCNNGG 4 cut(s) 58, 441, 447, 477
BseGI GGATG 3 cut(s) 359, 487, 620
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 2 cut(s) 72, 377
BseNI ACTGG 1 cut(s) 425
BseRI GAGGAG 1 cut(s) 446
BseXI GCAGC 2 cut(s) 168, 604
BshFI GGCC 1 cut(s) 446
BshTI ACCGGT 1 cut(s) 41
BsiHKAI GWGCWC 1 cut(s) 187
BsiSI CCGG 1 cut(s) 42
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 3 cut(s) 77, 161, 407
BsmFI GGGAC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 159
BsnI GGCC 1 cut(s) 446
Bsp1286I GDGCHC 1 cut(s) 187
Bsp143I GATC 2 cut(s) 212, 549
Bsp19I CCATGG 1 cut(s) 58
BspANI GGCC 1 cut(s) 446
BspCNI CTCAG 2 cut(s) 73, 376
BspLI GGNNCC 1 cut(s) 7
BspMAI CTGCAG 1 cut(s) 175
BspPI GGATC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 1 cut(s) 425
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 4 cut(s) 58, 441, 447, 477
BssMI GATC 2 cut(s) 212, 549
BssT1I CCWWGG 3 cut(s) 58, 441, 447
Bst6I CTCTTC 1 cut(s) 584
BstDEI CTNAG 3 cut(s) 81, 291, 363
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 3 cut(s) 359, 487, 620
BstH2I RGCGCY 1 cut(s) 496
BstHHI GCGC 1 cut(s) 495
BstKTI GATC 2 cut(s) 215, 552
BstMAI GTCTC 3 cut(s) 77, 161, 407
BstMBI GATC 2 cut(s) 212, 549
BstMWI GCNNNNNNNGC 2 cut(s) 501, 565
BstNSI RCATGY 1 cut(s) 572
BstPAI GACNNNNGTC 1 cut(s) 89
BstSFI CTRYAG 2 cut(s) 171, 588
BstV1I GCAGC 2 cut(s) 168, 604
BstXI CCANNNNNNTGG 1 cut(s) 448
BsuRI GGCC 1 cut(s) 446
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 3 cut(s) 359, 487, 620
BtsIMutI CAGTG 1 cut(s) 102
CfoI GCGC 1 cut(s) 495
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 14
Csp6I GTAC 2 cut(s) 263, 635
CspAI ACCGGT 1 cut(s) 41
CviAII CATG 3 cut(s) 35, 59, 569
CviJI RGCY 4 cut(s) 314, 326, 361, 446
CviKI_1 RGCY 4 cut(s) 314, 326, 361, 446
CviQI GTAC 2 cut(s) 263, 635
DdeI CTNAG 3 cut(s) 81, 291, 363
DpnI GATC 2 cut(s) 214, 551
DpnII GATC 2 cut(s) 212, 549
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
Eco130I CCWWGG 3 cut(s) 58, 441, 447
Eco147I AGGCCT 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 14
Eco57I CTGAAG 1 cut(s) 369
EcoO109I RGGNCCY 1 cut(s) 14
EcoT14I CCWWGG 3 cut(s) 58, 441, 447
ErhI CCWWGG 3 cut(s) 58, 441, 447
FaeI CATG 3 cut(s) 38, 62, 572
FalI AAGNNNNNCTT 4 cut(s) 171, 203, 400, 432
FaqI GGGAC 1 cut(s) 356
FatI CATG 3 cut(s) 34, 58, 568
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 157, 618
FokI GGATG 3 cut(s) 366, 494, 607
Fsp4HI GCNGC 2 cut(s) 157, 618
FspBI CTAG 1 cut(s) 629
GlaI GCGC 1 cut(s) 494
GluI GCNGC 2 cut(s) 157, 618
HaeII RGCGCY 1 cut(s) 496
HaeIII GGCC 1 cut(s) 446
HapII CCGG 1 cut(s) 42
HhaI GCGC 1 cut(s) 495
Hin1II CATG 3 cut(s) 38, 62, 572
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HinfI GANTC 2 cut(s) 73, 233
HpaII CCGG 1 cut(s) 42
HphI GGTGA 2 cut(s) 120, 481
Hpy166II GTNNAC 3 cut(s) 128, 265, 514
Hpy188I TCNGA 5 cut(s) 10, 70, 366, 388, 481
Hpy188III TCNNGA 2 cut(s) 164, 583
Hpy8I GTNNAC 3 cut(s) 128, 265, 514
HpyAV CCTTC 5 cut(s) 5, 223, 393, 451, 536
HpyF10VI GCNNNNNNNGC 2 cut(s) 501, 565
HpyF3I CTNAG 3 cut(s) 81, 291, 363
Hsp92II CATG 3 cut(s) 38, 62, 572
HspAI GCGC 1 cut(s) 493
Kzo9I GATC 2 cut(s) 212, 549
LmnI GCTCC 1 cut(s) 182
LpnPI CCDG 8 cut(s) 30, 55, 159, 215, 259, 354, 438, 474
Lsp1109I GCAGC 2 cut(s) 168, 604
LweI GCATC 3 cut(s) 316, 475, 629
MaeI CTAG 1 cut(s) 629
MaeIII GTNAC 1 cut(s) 37
MalI GATC 2 cut(s) 214, 551
MboI GATC 2 cut(s) 212, 549
MboII GAAGA 2 cut(s) 182, 571
MfeI CAATTG 1 cut(s) 87
MhlI GDGCHC 1 cut(s) 187
MluCI AATT 8 cut(s) 30, 53, 87, 241, 252, 396, 505, 611
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 7 cut(s) 372, 424, 460, 487, 585, 589, 625
MseI TTAA 2 cut(s) 48, 276
MslI CAYNNNNRTG 2 cut(s) 605, 646
MspI CCGG 1 cut(s) 42
MunI CAATTG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 501, 565
NcoI CCATGG 1 cut(s) 58
NdeII GATC 2 cut(s) 212, 549
NlaIII CATG 3 cut(s) 38, 62, 572
NlaIV GGNNCC 1 cut(s) 7
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 1 cut(s) 572
PceI AGGCCT 1 cut(s) 446
PctI GAATGC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 73
PinAI ACCGGT 1 cut(s) 41
PkrI GCNGC 2 cut(s) 158, 619
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PpuMI RGGWCCY 1 cut(s) 14
PshAI GACNNNNGTC 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 14
PspN4I GGNNCC 1 cut(s) 7
PspPI GGNCC 1 cut(s) 14
PspPPI RGGWCCY 1 cut(s) 14
PstI CTGCAG 1 cut(s) 175
RsaI GTAC 2 cut(s) 264, 636
RsaNI GTAC 2 cut(s) 263, 635
RseI CAYNNNNRTG 2 cut(s) 605, 646
SaqAI TTAA 2 cut(s) 48, 276
SatI GCNGC 2 cut(s) 157, 618
Sau3AI GATC 2 cut(s) 212, 549
Sau96I GGNCC 1 cut(s) 14
SchI GAGTC 1 cut(s) 227
SduI GDGCHC 1 cut(s) 187
SetI ASST 7 cut(s) 16, 139, 316, 328, 471, 479, 636
SfaNI GCATC 3 cut(s) 316, 475, 629
SfcI CTRYAG 2 cut(s) 171, 588
SinI GGWCC 1 cut(s) 14
SmiMI CAYNNNNRTG 2 cut(s) 605, 646
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 8 cut(s) 30, 53, 87, 241, 252, 396, 505, 611
SseBI AGGCCT 1 cut(s) 446
SspMI CTAG 1 cut(s) 629
StuI AGGCCT 1 cut(s) 446
StyI CCWWGG 3 cut(s) 58, 441, 447
TasI AATT 8 cut(s) 30, 53, 87, 241, 252, 396, 505, 611
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 2 cut(s) 48, 276
Tru9I TTAA 2 cut(s) 48, 276
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 2 cut(s) 156, 617
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 4 cut(s) 23, 336, 390, 623
TspRI CASTG 1 cut(s) 109
VpaK11BI GGWCC 1 cut(s) 14
XapI RAATTY 4 cut(s) 53, 241, 396, 611
XceI RCATGY 1 cut(s) 572
XmiI GTMKAC 1 cut(s) 513
XspI CTAG 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.