FvH4_6g12180

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009580
Physical Location & Seq
Forward (+)
7364919 .. 7366784
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g12180.t2

Sequence Viewer

Length: 630 bp
ATGGGTTCACCGGAAAGATTGTTTGGCCGGAAAAGAACCCTTCATGAGATCCTTGGAGGAGGTCTTGTTGCAGATGTGATCTTGTGGAGGCAGAAAGATGTGACAATGAGGATAATGTTAGTAGCTTTAGCTGCTTGGGTGGTGTTTGAGAAGTCTGGTTATACGTTGCTATCACTGGTCTCTAGTGTTCTTCTCCTCCTCTTTGTCGTTCTCTTTCTCTGGGCTAAATCGGCTGCCATTCTTAACCGGCCAGCTCCACCCCTGCCTGAATTGCAGTTATCAGAAGAAATGGTGAATGAAGCTGCCGCTATCATCCGGACTCGTGTAAATGCTTTGCTTTTGGTTTCACTAGATATTTCTCTTGGTAAGGACTCGAGGTTGTTCTTCAAAGTGGCGGCTTACCCGCTGCTGATTTATTTTGTTGGTGGCTTGACTGATTTCCTAACTTTGAGCTACACCAGCCTCGTCATTGTTCTGACAATACCAGCGTTCTATGAGAGGTGTGAAGATTATGTGGACAAGTATGTCATGATGGGGTACAGGAGATTGCTGCAATTGTATGTTAAACTAGATGAAGAATATGTGAACAGATTTCAGCACTTGGTTCTGGAGAAGAAGAAACTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

210

Amino Acids

23.82

Weight (kDa)

9.12

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 23 - 122 1.6e-25 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 524
AccIII TCCGGA 1 cut(s) 315
AciI CCGC 3 cut(s) 306, 395, 404
AclWI GGATC 1 cut(s) 43
AcoI YGGCCR 2 cut(s) 25, 248
AfaI GTAC 1 cut(s) 539
AgsI TTSAA 1 cut(s) 388
AjuI GAANNNNNNNTTGG 1 cut(s) 38
AluBI AGCT 5 cut(s) 125, 131, 254, 302, 453
AluI AGCT 5 cut(s) 125, 131, 254, 302, 453
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 315
AoxI GGCC 2 cut(s) 25, 248
ApeKI GCWGC 5 cut(s) 131, 233, 302, 406, 550
AsuHPI GGTGA 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 373
BauI CACGAG 1 cut(s) 321
BbvI GCAGC 5 cut(s) 118, 220, 289, 393, 537
BccI CCATC 1 cut(s) 526
BcoDI GTCTC 1 cut(s) 184
BfaI CTAG 3 cut(s) 183, 350, 569
BisI GCNGC 7 cut(s) 132, 234, 303, 306, 396, 407, 551
BlsI GCNGC 7 cut(s) 133, 235, 304, 307, 397, 408, 552
BmeT110I CYCGRG 1 cut(s) 373
BpmI CTGGAG 1 cut(s) 629
BsaI GGTCTC 1 cut(s) 184
BsaJI CCNNGG 1 cut(s) 52
BsaWI WCCGGW 2 cut(s) 10, 315
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
Bse118I RCCGGY 1 cut(s) 246
Bse1I ACTGG 1 cut(s) 180
BseAI TCCGGA 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 180
BseRI GAGGAG 3 cut(s) 72, 185, 188
BseXI GCAGC 5 cut(s) 118, 220, 289, 393, 537
BshFI GGCC 2 cut(s) 27, 250
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 4 cut(s) 11, 28, 247, 316
BsmAI GTCTC 1 cut(s) 184
BsnI GGCC 2 cut(s) 27, 250
Bso31I GGTCTC 1 cut(s) 184
BsoBI CYCGRG 1 cut(s) 373
Bsp13I TCCGGA 1 cut(s) 315
Bsp143I GATC 2 cut(s) 48, 78
BspACI CCGC 3 cut(s) 306, 395, 404
BspANI GGCC 2 cut(s) 27, 250
BspEI TCCGGA 1 cut(s) 315
BspHI TCATGA 2 cut(s) 43, 528
BspPI GGATC 1 cut(s) 43
BspTNI GGTCTC 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 246
BsrI ACTGG 1 cut(s) 180
BssAI RCCGGY 1 cut(s) 246
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 2 cut(s) 48, 78
BssSI CACGAG 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 52
Bst2BI CACGAG 1 cut(s) 321
BstC8I GCNNGC 1 cut(s) 252
BstDEI CTNAG 1 cut(s) 623
BstF5I GGATG 1 cut(s) 312
BstKTI GATC 2 cut(s) 51, 81
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 2 cut(s) 48, 78
BstMWI GCNNNNNNNGC 4 cut(s) 131, 230, 271, 459
BstV1I GCAGC 5 cut(s) 118, 220, 289, 393, 537
BstX2I RGATCY 1 cut(s) 48
BstYI RGATCY 1 cut(s) 48
BsuRI GGCC 2 cut(s) 27, 250
BtsCI GGATG 1 cut(s) 312
BtsIMutI CAGTG 1 cut(s) 173
Cac8I GCNNGC 1 cut(s) 252
CciI TCATGA 2 cut(s) 43, 528
Cfr10I RCCGGY 1 cut(s) 246
Csp6I GTAC 1 cut(s) 538
CviAII CATG 2 cut(s) 44, 529
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 1 cut(s) 623
DpnI GATC 2 cut(s) 50, 80
DpnII GATC 2 cut(s) 48, 78
DrdI GACNNNNNNGTC 1 cut(s) 524
DseDI GACNNNNNNGTC 1 cut(s) 524
EaeI YGGCCR 2 cut(s) 25, 248
Eco130I CCWWGG 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
FaeI CATG 2 cut(s) 47, 532
FaiI YATR 8 cut(s) 45, 162, 495, 513, 525, 530, 561, 582
FatI CATG 2 cut(s) 43, 528
FauI CCCGC 1 cut(s) 411
Fnu4HI GCNGC 7 cut(s) 132, 234, 303, 306, 396, 407, 551
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 7 cut(s) 132, 234, 303, 306, 396, 407, 551
FspBI CTAG 3 cut(s) 183, 350, 569
GluI GCNGC 7 cut(s) 132, 234, 303, 306, 396, 407, 551
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 2 cut(s) 27, 250
HapII CCGG 4 cut(s) 11, 28, 247, 316
Hin1II CATG 2 cut(s) 47, 532
HinfI GANTC 2 cut(s) 319, 371
HpaII CCGG 4 cut(s) 11, 28, 247, 316
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 3 cut(s) 8, 517, 586
Hpy188I TCNGA 2 cut(s) 283, 477
Hpy188III TCNNGA 4 cut(s) 44, 316, 529, 608
Hpy8I GTNNAC 3 cut(s) 8, 517, 586
HpyAV CCTTC 1 cut(s) 50
HpyCH4IV ACGT 1 cut(s) 164
HpyCH4V TGCA 3 cut(s) 71, 274, 553
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 230, 271, 459
HpyF3I CTNAG 1 cut(s) 623
HpySE526I ACGT 1 cut(s) 164
Hsp92II CATG 2 cut(s) 47, 532
Kpn2I TCCGGA 1 cut(s) 315
Kzo9I GATC 2 cut(s) 48, 78
LmnI GCTCC 1 cut(s) 259
Lsp1109I GCAGC 5 cut(s) 118, 220, 289, 393, 537
MaeI CTAG 3 cut(s) 183, 350, 569
MaeII ACGT 1 cut(s) 164
MaeIII GTNAC 1 cut(s) 100
MalI GATC 2 cut(s) 50, 80
MboI GATC 2 cut(s) 48, 78
MboII GAAGA 7 cut(s) 182, 296, 376, 518, 587, 625, 628
MfeI CAATTG 1 cut(s) 554
MflI RGATCY 1 cut(s) 48
MluCI AATT 2 cut(s) 269, 554
MlyI GAGTC 2 cut(s) 313, 365
MnlI CCTC 9 cut(s) 50, 53, 81, 102, 206, 209, 369, 473, 492
MroI TCCGGA 1 cut(s) 315
MseI TTAA 2 cut(s) 243, 564
MspA1I CMGCKG 1 cut(s) 406
MspI CCGG 4 cut(s) 11, 28, 247, 316
MunI CAATTG 1 cut(s) 554
MwoI GCNNNNNNNGC 4 cut(s) 131, 230, 271, 459
NdeII GATC 2 cut(s) 48, 78
NlaIII CATG 2 cut(s) 47, 532
NmuCI GTSAC 1 cut(s) 100
PaeR7I CTCGAG 1 cut(s) 373
PagI TCATGA 2 cut(s) 43, 528
PkrI GCNGC 7 cut(s) 133, 235, 304, 307, 397, 408, 552
PleI GAGTC 2 cut(s) 313, 365
PpsI GAGTC 2 cut(s) 313, 365
PspXI VCTCGAGB 1 cut(s) 373
PsuI RGATCY 1 cut(s) 48
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
SaqAI TTAA 2 cut(s) 243, 564
SatI GCNGC 7 cut(s) 132, 234, 303, 306, 396, 407, 551
Sau3AI GATC 2 cut(s) 48, 78
SchI GAGTC 2 cut(s) 313, 365
SetI ASST 9 cut(s) 64, 127, 133, 167, 256, 304, 380, 455, 503
Sfr274I CTCGAG 1 cut(s) 373
SlaI CTCGAG 1 cut(s) 373
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 2 cut(s) 269, 554
SsiI CCGC 3 cut(s) 306, 395, 404
SspMI CTAG 3 cut(s) 183, 350, 569
StyI CCWWGG 1 cut(s) 52
TaiI ACGT 1 cut(s) 167
TaqI TCGA 1 cut(s) 374
TasI AATT 2 cut(s) 269, 554
TauI GCSGC 2 cut(s) 308, 398
Tru1I TTAA 2 cut(s) 243, 564
Tru9I TTAA 2 cut(s) 243, 564
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 5 cut(s) 131, 233, 302, 406, 550
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 3 cut(s) 32, 312, 588
TspRI CASTG 1 cut(s) 180
XhoI CTCGAG 1 cut(s) 373
XspI CTAG 3 cut(s) 183, 350, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.