MD04G1146100.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009580
Physical Location & Seq
Reverse (-)
23465987 .. 23467762
1776 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1146100.v1.1.491

Sequence Viewer

Length: 630 bp
ATGGGTTCATCCGAAAGGTTGTTCAACAGGCAGAGAACCCTTCACGAGATCCTTGGAGCAAGTCTTGTTGCAGACGTGATTCTGTGGAGGCAGAAGAATGTGACTTTGGTGATACTGTTAGTAACTCTATCTGTTTGGCTGGTGTTTGAGAAGTCTGGTTACACTCTGCTATCACTTGCATCCAGTGTTCTTCTCCTCCTCATCACAACTCTCTTTCTCTGGGCTAAATCCGCCGCCGTTCTTAACCGACCAGCTCCTCCCCTGCCTGAATTGCATTTATCGGAAGAAATGGCAAACGAAATGGCATCTTTCATCCGCATTCGTTTAAACGCTTTGCTCTCGGGTTTCCAAAATATTTCTCTGGGAAAGGACTCGAGATTGTTCTTCAAAGTAGCTACATCTCTCTTGGTGATCTACTTTGTTGGCGGCTTGACTGATTTCCTTACTTTGAGCTACACCAGTCTCGTCATTGTTCTAACAATTCCGGCGCTCTACGAGAGGTGTGAGGATTATGTGGACAAATATGTGATGATGGGGTACAGGAAGTTGCTGCAGTTGTATGTTAAGCTAGATTATGAGTATGTAAGCAGATTTCAAAACGAGGATTTGGTGAAGAAGAAATTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

210

Amino Acids

23.84

Weight (kDa)

9.12

Isoelectric Point (pI)

28.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 23 - 177 1.4e-47 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 231, 234, 316, 426
AclWI GGATC 1 cut(s) 43
AfaI GTAC 1 cut(s) 539
AgsI TTSAA 3 cut(s) 25, 388, 596
AjiI CACGTC 1 cut(s) 76
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AluBI AGCT 4 cut(s) 254, 395, 453, 568
AluI AGCT 4 cut(s) 254, 395, 453, 568
Alw26I GTCTC 1 cut(s) 467
AlwI GGATC 1 cut(s) 43
Ama87I CYCGRG 2 cut(s) 340, 373
ApeKI GCWGC 1 cut(s) 550
AspLEI GCGC 1 cut(s) 490
AsuHPI GGTGA 3 cut(s) 121, 421, 622
AvaI CYCGRG 2 cut(s) 340, 373
BarI GAAGNNNNNNTAC 2 cut(s) 143, 175
BauI CACGAG 1 cut(s) 44
BbvI GCAGC 1 cut(s) 537
BccI CCATC 1 cut(s) 526
BceAI ACGGC 1 cut(s) 221
BcoDI GTCTC 1 cut(s) 467
BfaI CTAG 1 cut(s) 569
BfmI CTRYAG 1 cut(s) 551
BfoI RGCGCY 1 cut(s) 491
BisI GCNGC 3 cut(s) 234, 427, 551
BlsI GCNGC 3 cut(s) 235, 428, 552
BmeT110I CYCGRG 2 cut(s) 340, 373
BmgBI CACGTC 1 cut(s) 76
BmsI GCATC 2 cut(s) 188, 314
BsaJI CCNNGG 1 cut(s) 52
Bse1I ACTGG 2 cut(s) 183, 459
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 3 cut(s) 8, 179, 312
BseNI ACTGG 2 cut(s) 183, 459
BseRI GAGGAG 3 cut(s) 185, 188, 246
BseXI GCAGC 1 cut(s) 537
BsiHKCI CYCGRG 2 cut(s) 340, 373
BsiSI CCGG 1 cut(s) 485
BsmAI GTCTC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 318
BsoBI CYCGRG 2 cut(s) 340, 373
Bsp143I GATC 2 cut(s) 48, 411
BspACI CCGC 4 cut(s) 231, 234, 316, 426
BspMAI CTGCAG 1 cut(s) 555
BspPI GGATC 1 cut(s) 43
BsrI ACTGG 2 cut(s) 183, 459
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 2 cut(s) 48, 411
BssSI CACGAG 1 cut(s) 44
BssT1I CCWWGG 1 cut(s) 52
Bst2BI CACGAG 1 cut(s) 44
Bst4CI ACNGT 1 cut(s) 117
BstF5I GGATG 3 cut(s) 8, 179, 312
BstH2I RGCGCY 1 cut(s) 491
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 2 cut(s) 51, 414
BstMAI GTCTC 1 cut(s) 467
BstMBI GATC 2 cut(s) 48, 411
BstMWI GCNNNNNNNGC 2 cut(s) 230, 271
BstSFI CTRYAG 1 cut(s) 551
BstV1I GCAGC 1 cut(s) 537
BstX2I RGATCY 1 cut(s) 48
BstYI RGATCY 1 cut(s) 48
BtrI CACGTC 1 cut(s) 76
BtsCI GGATG 3 cut(s) 8, 179, 312
BtsIMutI CAGTG 1 cut(s) 190
CfoI GCGC 1 cut(s) 490
Csp6I GTAC 1 cut(s) 538
CviJI RGCY 7 cut(s) 139, 224, 254, 395, 429, 453, 568
CviKI_1 RGCY 7 cut(s) 139, 224, 254, 395, 429, 453, 568
CviQI GTAC 1 cut(s) 538
DpnI GATC 2 cut(s) 50, 413
DpnII GATC 2 cut(s) 48, 411
DraI TTTAAA 1 cut(s) 327
EciI GGCGGA 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 52
Eco88I CYCGRG 2 cut(s) 340, 373
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
FaiI YATR 5 cut(s) 513, 525, 561, 576, 582
Fnu4HI GCNGC 3 cut(s) 234, 427, 551
FokI GGATG 2 cut(s) 166, 299
Fsp4HI GCNGC 3 cut(s) 234, 427, 551
FspBI CTAG 1 cut(s) 569
GlaI GCGC 1 cut(s) 489
GluI GCNGC 3 cut(s) 234, 427, 551
HaeII RGCGCY 1 cut(s) 491
HapII CCGG 1 cut(s) 485
HhaI GCGC 1 cut(s) 490
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HinfI GANTC 2 cut(s) 79, 371
HpaII CCGG 1 cut(s) 485
HphI GGTGA 3 cut(s) 121, 421, 622
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 2 cut(s) 13, 283
Hpy188III TCNNGA 2 cut(s) 44, 375
Hpy8I GTNNAC 1 cut(s) 517
HpyAV CCTTC 1 cut(s) 50
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 4 cut(s) 71, 179, 274, 553
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 271
HpySE526I ACGT 1 cut(s) 75
HspAI GCGC 1 cut(s) 488
Kzo9I GATC 2 cut(s) 48, 411
LmnI GCTCC 2 cut(s) 56, 259
Lsp1109I GCAGC 1 cut(s) 537
LweI GCATC 2 cut(s) 188, 314
MaeI CTAG 1 cut(s) 569
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 3 cut(s) 100, 121, 158
MalI GATC 2 cut(s) 50, 413
MboI GATC 2 cut(s) 48, 411
MboII GAAGA 6 cut(s) 106, 182, 296, 376, 625, 628
MflI RGATCY 1 cut(s) 48
MluCI AATT 3 cut(s) 269, 480, 620
MlyI GAGTC 1 cut(s) 365
MnlI CCTC 7 cut(s) 81, 206, 209, 267, 492, 499, 595
MseI TTAA 4 cut(s) 243, 326, 564, 623
MspI CCGG 1 cut(s) 485
MssI GTTTAAAC 1 cut(s) 327
Mva1269I GAATGC 1 cut(s) 318
MwoI GCNNNNNNNGC 2 cut(s) 230, 271
NdeII GATC 2 cut(s) 48, 411
NmuCI GTSAC 1 cut(s) 100
PaeR7I CTCGAG 1 cut(s) 373
PctI GAATGC 1 cut(s) 318
PfeI GAWTC 1 cut(s) 79
PkrI GCNGC 3 cut(s) 235, 428, 552
PleI GAGTC 1 cut(s) 365
PmeI GTTTAAAC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 365
PstI CTGCAG 1 cut(s) 555
PsuI RGATCY 1 cut(s) 48
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
SaqAI TTAA 4 cut(s) 243, 326, 564, 623
SatI GCNGC 3 cut(s) 234, 427, 551
Sau3AI GATC 2 cut(s) 48, 411
SchI GAGTC 1 cut(s) 365
SetI ASST 7 cut(s) 20, 78, 256, 397, 455, 503, 570
SfaNI GCATC 2 cut(s) 188, 314
SfcI CTRYAG 1 cut(s) 551
Sfr274I CTCGAG 1 cut(s) 373
SlaI CTCGAG 1 cut(s) 373
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 3 cut(s) 269, 480, 620
SsiI CCGC 4 cut(s) 231, 234, 316, 426
SspI AATATT 1 cut(s) 355
SspMI CTAG 1 cut(s) 569
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 1 cut(s) 117
TaiI ACGT 1 cut(s) 78
TaqI TCGA 1 cut(s) 374
TasI AATT 3 cut(s) 269, 480, 620
TauI GCSGC 2 cut(s) 236, 429
TfiI GAWTC 1 cut(s) 79
Tru1I TTAA 4 cut(s) 243, 326, 564, 623
Tru9I TTAA 4 cut(s) 243, 326, 564, 623
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 1 cut(s) 550
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 1 cut(s) 301
TspRI CASTG 1 cut(s) 190
XhoI CTCGAG 1 cut(s) 373
XspI CTAG 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.