MD12G1159300.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009580
Physical Location & Seq
Reverse (-)
23937843 .. 23939768
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1159300.v1.1.491

Sequence Viewer

Length: 630 bp
ATGACTTCATCTGAAAGGTTGTTCAACAGGCAAAGAACACTTCATGAGATCCTTGGAGGAGGTCTTGTTGCAGATGTGATTCTGTGGAGGCAGAAAAATGTGACTTTGGGGATAATCCTAGTAACTTTATCTGTTTGGGTGGTGTTTGAGAAGTCTGGTTACACTCTGCTGTCACTTGGCTCAAGTGTTCTCCTCCTCCTCATGACCACTCTCTTTCTCTGGGCTAAATCCGCCGCCATTCTTAACCGACCAGCTCCTCCCCTGCCTAAATTGCATTTATCAGAAGAAATGGTAAACGAAATGGCATCTTTCATCCGCATTCATGTAAACGCTTTGCTCTCGGCTTTCCAAGATATTTCTCTCGGAAAGGACTCAAGGTCGTTCTTCAAAGTAGCTGCATCCCTCTTGGTGATTTACCTTGTTGGTGGCTGGACTGATTTTCTTACTTTGAGCTACACCTGTCTTGCGGTCGTTCTAACAGTTCCAGCGCTCTACGAGAGGTGTGAAGATTACGTGGACAAATATGTCATGATGGGGTACCTGAAATTGCTGCAGTTGTACGTTAAACTAGATTATGAGTATGTAAACAGACTTCAACACGAGGATTTGGAGAAGAAGAAACTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

210

Amino Acids

23.75

Weight (kDa)

8.45

Isoelectric Point (pI)

32.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 23 - 177 1e-48 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 524
Acc65I GGTACC 1 cut(s) 537
AccB1I GGYRCC 1 cut(s) 537
AciI CCGC 4 cut(s) 231, 234, 316, 467
AclWI GGATC 1 cut(s) 43
AfaI GTAC 2 cut(s) 539, 560
AfeI AGCGCT 1 cut(s) 489
AgsI TTSAA 3 cut(s) 25, 388, 596
AhdI GACNNNNNGTC 1 cut(s) 376
AluBI AGCT 3 cut(s) 254, 395, 453
AluI AGCT 3 cut(s) 254, 395, 453
AlwI GGATC 1 cut(s) 43
Aor51HI AGCGCT 1 cut(s) 489
ApeKI GCWGC 2 cut(s) 395, 550
Asp718I GGTACC 1 cut(s) 537
AspLEI GCGC 1 cut(s) 490
AsuHPI GGTGA 1 cut(s) 421
BanI GGYRCC 1 cut(s) 537
BarI GAAGNNNNNNTAC 4 cut(s) 143, 175, 576, 608
BauI CACGAG 1 cut(s) 599
BbvI GCAGC 2 cut(s) 382, 537
BccI CCATC 1 cut(s) 526
BfaI CTAG 2 cut(s) 119, 569
BfmI CTRYAG 1 cut(s) 551
BfoI RGCGCY 1 cut(s) 491
BisI GCNGC 3 cut(s) 234, 396, 551
BlsI GCNGC 3 cut(s) 235, 397, 552
BmeRI GACNNNNNGTC 1 cut(s) 376
BmiI GGNNCC 1 cut(s) 539
BmsI GCATC 2 cut(s) 314, 407
BpuEI CTTGAG 2 cut(s) 166, 358
BsaAI YACGTR 1 cut(s) 514
BsaJI CCNNGG 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 2 cut(s) 312, 398
BseRI GAGGAG 5 cut(s) 72, 182, 185, 188, 246
BseXI GCAGC 2 cut(s) 382, 537
Bsh1285I CGRYCG 1 cut(s) 471
BshNI GGYRCC 1 cut(s) 537
BsiEI CGRYCG 1 cut(s) 471
BsmI GAATGC 1 cut(s) 318
Bsp143I GATC 1 cut(s) 48
BspACI CCGC 4 cut(s) 231, 234, 316, 467
BspHI TCATGA 3 cut(s) 43, 201, 528
BspLI GGNNCC 1 cut(s) 539
BspMAI CTGCAG 1 cut(s) 555
BspPI GGATC 1 cut(s) 43
BspT107I GGYRCC 1 cut(s) 537
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 1 cut(s) 48
BssSI CACGAG 1 cut(s) 599
BssT1I CCWWGG 1 cut(s) 52
Bst2BI CACGAG 1 cut(s) 599
Bst4CI ACNGT 1 cut(s) 481
BstBAI YACGTR 1 cut(s) 514
BstDEI CTNAG 1 cut(s) 623
BstF5I GGATG 2 cut(s) 312, 398
BstH2I RGCGCY 1 cut(s) 491
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 1 cut(s) 51
BstMBI GATC 1 cut(s) 48
BstMCI CGRYCG 1 cut(s) 471
BstMWI GCNNNNNNNGC 2 cut(s) 230, 271
BstSFI CTRYAG 1 cut(s) 551
BstV1I GCAGC 2 cut(s) 382, 537
BstX2I RGATCY 1 cut(s) 48
BstYI RGATCY 1 cut(s) 48
BtsCI GGATG 2 cut(s) 312, 398
CciI TCATGA 3 cut(s) 43, 201, 528
CfoI GCGC 1 cut(s) 490
Csp6I GTAC 2 cut(s) 538, 559
CviAII CATG 4 cut(s) 44, 202, 323, 529
CviJI RGCY 7 cut(s) 180, 224, 254, 344, 395, 429, 453
CviKI_1 RGCY 7 cut(s) 180, 224, 254, 344, 395, 429, 453
CviQI GTAC 2 cut(s) 538, 559
DdeI CTNAG 1 cut(s) 623
DpnI GATC 1 cut(s) 50
DpnII GATC 1 cut(s) 48
DrdI GACNNNNNNGTC 1 cut(s) 524
DriI GACNNNNNGTC 1 cut(s) 376
DseDI GACNNNNNNGTC 1 cut(s) 524
Eam1105I GACNNNNNGTC 1 cut(s) 376
EciI GGCGGA 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 52
Eco47III AGCGCT 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
FaeI CATG 4 cut(s) 47, 205, 326, 532
FaiI YATR 7 cut(s) 45, 203, 324, 525, 530, 576, 582
FatI CATG 4 cut(s) 43, 201, 322, 528
Fnu4HI GCNGC 3 cut(s) 234, 396, 551
FokI GGATG 2 cut(s) 299, 385
Fsp4HI GCNGC 3 cut(s) 234, 396, 551
FspBI CTAG 2 cut(s) 119, 569
GlaI GCGC 1 cut(s) 489
GluI GCNGC 3 cut(s) 234, 396, 551
HaeII RGCGCY 1 cut(s) 491
HhaI GCGC 1 cut(s) 490
Hin1II CATG 4 cut(s) 47, 205, 326, 532
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HinfI GANTC 2 cut(s) 79, 371
HphI GGTGA 1 cut(s) 421
Hpy166II GTNNAC 4 cut(s) 295, 328, 517, 586
Hpy188I TCNGA 3 cut(s) 13, 283, 365
Hpy188III TCNNGA 3 cut(s) 44, 202, 529
Hpy8I GTNNAC 4 cut(s) 295, 328, 517, 586
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4IV ACGT 2 cut(s) 513, 561
HpyCH4V TGCA 4 cut(s) 71, 274, 398, 553
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 271
HpyF3I CTNAG 1 cut(s) 623
HpySE526I ACGT 2 cut(s) 513, 561
Hsp92II CATG 4 cut(s) 47, 205, 326, 532
HspAI GCGC 1 cut(s) 488
KpnI GGTACC 1 cut(s) 541
Kzo9I GATC 1 cut(s) 48
LmnI GCTCC 1 cut(s) 259
LpnPI CCDG 9 cut(s) 13, 141, 205, 264, 275, 415, 472, 498, 554
Lsp1109I GCAGC 2 cut(s) 382, 537
LweI GCATC 2 cut(s) 314, 407
MaeI CTAG 2 cut(s) 119, 569
MaeII ACGT 2 cut(s) 513, 561
MaeIII GTNAC 4 cut(s) 100, 121, 158, 171
MalI GATC 1 cut(s) 50
MboI GATC 1 cut(s) 48
MboII GAAGA 5 cut(s) 296, 376, 518, 625, 628
MflI RGATCY 1 cut(s) 48
MluCI AATT 2 cut(s) 269, 545
MlyI GAGTC 1 cut(s) 365
MseI TTAA 2 cut(s) 243, 564
Mva1269I GAATGC 1 cut(s) 318
MwoI GCNNNNNNNGC 2 cut(s) 230, 271
NdeII GATC 1 cut(s) 48
NlaIII CATG 4 cut(s) 47, 205, 326, 532
NlaIV GGNNCC 1 cut(s) 539
NmeAIII GCCGAG 1 cut(s) 320
NmuCI GTSAC 2 cut(s) 100, 171
PagI TCATGA 3 cut(s) 43, 201, 528
PctI GAATGC 1 cut(s) 318
PfeI GAWTC 1 cut(s) 79
PkrI GCNGC 3 cut(s) 235, 397, 552
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
Ppu21I YACGTR 1 cut(s) 514
PspN4I GGNNCC 1 cut(s) 539
PstI CTGCAG 1 cut(s) 555
PsuI RGATCY 1 cut(s) 48
RsaI GTAC 2 cut(s) 539, 560
RsaNI GTAC 2 cut(s) 538, 559
SaqAI TTAA 2 cut(s) 243, 564
SatI GCNGC 3 cut(s) 234, 396, 551
Sau3AI GATC 1 cut(s) 48
SchI GAGTC 1 cut(s) 365
SfaNI GCATC 2 cut(s) 314, 407
SfcI CTRYAG 1 cut(s) 551
SmlI CTYRAG 2 cut(s) 181, 373
SmoI CTYRAG 2 cut(s) 181, 373
Sse9I AATT 2 cut(s) 269, 545
SsiI CCGC 4 cut(s) 231, 234, 316, 467
SspMI CTAG 2 cut(s) 119, 569
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 1 cut(s) 481
TaiI ACGT 2 cut(s) 516, 564
TasI AATT 2 cut(s) 269, 545
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 1 cut(s) 79
Tru1I TTAA 2 cut(s) 243, 564
Tru9I TTAA 2 cut(s) 243, 564
TseFI GTSAC 2 cut(s) 100, 171
TseI GCWGC 2 cut(s) 395, 550
Tsp45I GTSAC 2 cut(s) 100, 171
TspDTI ATGAA 3 cut(s) 32, 301, 311
XspI CTAG 2 cut(s) 119, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.