RLG00000024730

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009580
Physical Location & Seq
Reverse (-)
38604562 .. 38606288
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024730

Sequence Viewer

Length: 630 bp
ATGGGTTCACCGGAAAGATTGTTTCGCCGGCAAAGAACCCTTCATGAGATCCTTGGAGGAGGTCTTGTTGCAGATGTGATCTTGTGGAGGCAGAAAGATGTGACAATGGGGATACTGTTAGTAGCTTTAGCTGCTTGGGTGGTGTTTGAGAAGTCTGGTTATACATTGCTATCACTGGTCTCTAGTGTTCTCCTCCTCCTTTTTGTCATACTCTTTCTCTGGGCTAAATCGGCTGCCATTCTTAACCGACCAGCTCCACCCCTGCCTGAATTGCAGTTATCAGAAGAAATGGTGAATGAAGCTGCAGCTGTCATCCGGACTCATGTAAATGCTTTGCTTTTGGTTTCACAAGATATTTCTCTTGGTAAAGACTCGAGGTTGTTCTTCAAAGTAGCTGCTTACCTGCTGCTGATTTATTTTGTTGGTGGCTTTACTGATTTCCTGACTCTGAGCTACACCAGCCTTGTCATTGTTCTAACAATTCCAGCATTCTATGAGAGATATGAAGATTATGTGGACAAGTATGTCATGATGGGGTACAGGAAATTGCTGCAATTGTATGTTAAACTAGATGAAGAATATGCGAGCAGATTTCAACACTTGGTTCTGGAGAAGAAGAAACTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

210

Amino Acids

23.82

Weight (kDa)

8.69

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 23 - 177 1.1e-49 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 524
Acc36I ACCTGC 1 cut(s) 411
AccIII TCCGGA 1 cut(s) 315
AclWI GGATC 1 cut(s) 43
AfaI GTAC 1 cut(s) 539
AgsI TTSAA 2 cut(s) 388, 596
AluBI AGCT 7 cut(s) 125, 131, 254, 302, 308, 395, 453
AluI AGCT 7 cut(s) 125, 131, 254, 302, 308, 395, 453
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 315
ApeKI GCWGC 7 cut(s) 131, 233, 302, 305, 395, 406, 550
AsuHPI GGTGA 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 373
BbvI GCAGC 7 cut(s) 118, 220, 289, 317, 382, 393, 537
BccI CCATC 1 cut(s) 526
BciVI GTATCC 1 cut(s) 105
BcoDI GTCTC 1 cut(s) 184
BfaI CTAG 2 cut(s) 183, 569
BfmI CTRYAG 1 cut(s) 303
BfuAI ACCTGC 1 cut(s) 411
BfuI GTATCC 1 cut(s) 105
BisI GCNGC 7 cut(s) 132, 234, 303, 306, 396, 407, 551
BlsI GCNGC 7 cut(s) 133, 235, 304, 307, 397, 408, 552
BmeT110I CYCGRG 1 cut(s) 373
BpmI CTGGAG 1 cut(s) 629
BsaI GGTCTC 1 cut(s) 184
BsaJI CCNNGG 1 cut(s) 52
BsaWI WCCGGW 2 cut(s) 10, 315
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
Bse118I RCCGGY 1 cut(s) 27
Bse1I ACTGG 1 cut(s) 180
Bse3DI GCAATG 1 cut(s) 164
BseAI TCCGGA 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 1 cut(s) 312
BseMI GCAATG 1 cut(s) 164
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 1 cut(s) 180
BseRI GAGGAG 3 cut(s) 72, 182, 185
BseXI GCAGC 7 cut(s) 118, 220, 289, 317, 382, 393, 537
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 3 cut(s) 11, 28, 316
BsmAI GTCTC 1 cut(s) 184
BsmI GAATGC 1 cut(s) 488
Bso31I GGTCTC 1 cut(s) 184
BsoBI CYCGRG 1 cut(s) 373
Bsp13I TCCGGA 1 cut(s) 315
Bsp143I GATC 2 cut(s) 48, 78
BspCNI CTCAG 1 cut(s) 441
BspEI TCCGGA 1 cut(s) 315
BspHI TCATGA 2 cut(s) 43, 528
BspMAI CTGCAG 1 cut(s) 307
BspMI ACCTGC 1 cut(s) 411
BspPI GGATC 1 cut(s) 43
BspTNI GGTCTC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 27
BsrI ACTGG 1 cut(s) 180
BssAI RCCGGY 1 cut(s) 27
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 2 cut(s) 48, 78
BssT1I CCWWGG 1 cut(s) 52
Bst4CI ACNGT 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 29, 586
BstDEI CTNAG 2 cut(s) 449, 623
BstF5I GGATG 1 cut(s) 312
BstKTI GATC 2 cut(s) 51, 81
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 2 cut(s) 48, 78
BstMWI GCNNNNNNNGC 4 cut(s) 131, 230, 271, 459
BstSFI CTRYAG 1 cut(s) 303
BstV1I GCAGC 7 cut(s) 118, 220, 289, 317, 382, 393, 537
BstX2I RGATCY 1 cut(s) 48
BstYI RGATCY 1 cut(s) 48
BsuI GTATCC 1 cut(s) 105
BtsCI GGATG 1 cut(s) 312
BtsIMutI CAGTG 1 cut(s) 173
BveI ACCTGC 1 cut(s) 411
Cac8I GCNNGC 2 cut(s) 29, 586
CciI TCATGA 2 cut(s) 43, 528
Cfr10I RCCGGY 1 cut(s) 27
Csp6I GTAC 1 cut(s) 538
CviAII CATG 3 cut(s) 44, 323, 529
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 2 cut(s) 449, 623
DpnI GATC 2 cut(s) 50, 80
DpnII GATC 2 cut(s) 48, 78
DrdI GACNNNNNNGTC 1 cut(s) 524
DseDI GACNNNNNNGTC 1 cut(s) 524
Eco130I CCWWGG 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
FaeI CATG 3 cut(s) 47, 326, 532
FatI CATG 3 cut(s) 43, 322, 528
Fnu4HI GCNGC 7 cut(s) 132, 234, 303, 306, 396, 407, 551
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 7 cut(s) 132, 234, 303, 306, 396, 407, 551
FspBI CTAG 2 cut(s) 183, 569
GluI GCNGC 7 cut(s) 132, 234, 303, 306, 396, 407, 551
GsuI CTGGAG 1 cut(s) 629
HapII CCGG 3 cut(s) 11, 28, 316
Hin1II CATG 3 cut(s) 47, 326, 532
HinfI GANTC 3 cut(s) 319, 371, 445
HpaII CCGG 3 cut(s) 11, 28, 316
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 2 cut(s) 8, 517
Hpy188I TCNGA 2 cut(s) 283, 450
Hpy188III TCNNGA 5 cut(s) 44, 316, 442, 529, 608
Hpy8I GTNNAC 2 cut(s) 8, 517
HpyAV CCTTC 1 cut(s) 50
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4V TGCA 4 cut(s) 71, 274, 305, 553
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 230, 271, 459
HpyF3I CTNAG 2 cut(s) 449, 623
Hsp92II CATG 3 cut(s) 47, 326, 532
Kpn2I TCCGGA 1 cut(s) 315
KroI GCCGGC 1 cut(s) 27
KroNI GCCGGC 1 cut(s) 29
Kzo9I GATC 2 cut(s) 48, 78
LmnI GCTCC 1 cut(s) 259
Lsp1109I GCAGC 7 cut(s) 118, 220, 289, 317, 382, 393, 537
MaeI CTAG 2 cut(s) 183, 569
MaeIII GTNAC 1 cut(s) 100
MalI GATC 2 cut(s) 50, 80
MboI GATC 2 cut(s) 48, 78
MboII GAAGA 6 cut(s) 296, 376, 518, 587, 625, 628
MfeI CAATTG 1 cut(s) 554
MflI RGATCY 1 cut(s) 48
MluCI AATT 4 cut(s) 269, 480, 545, 554
MlyI GAGTC 3 cut(s) 313, 365, 439
MnlI CCTC 6 cut(s) 50, 53, 81, 203, 206, 369
MroI TCCGGA 1 cut(s) 315
MroNI GCCGGC 1 cut(s) 27
MseI TTAA 2 cut(s) 243, 564
MslI CAYNNNNRTG 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 3 cut(s) 11, 28, 316
MunI CAATTG 1 cut(s) 554
Mva1269I GAATGC 1 cut(s) 488
MwoI GCNNNNNNNGC 4 cut(s) 131, 230, 271, 459
NaeI GCCGGC 1 cut(s) 29
NdeII GATC 2 cut(s) 48, 78
NgoMIV GCCGGC 1 cut(s) 27
NlaIII CATG 3 cut(s) 47, 326, 532
NmuCI GTSAC 1 cut(s) 100
PaeR7I CTCGAG 1 cut(s) 373
PagI TCATGA 2 cut(s) 43, 528
PctI GAATGC 1 cut(s) 488
PdiI GCCGGC 1 cut(s) 29
PkrI GCNGC 7 cut(s) 133, 235, 304, 307, 397, 408, 552
PleI GAGTC 3 cut(s) 313, 365, 439
PpsI GAGTC 3 cut(s) 313, 365, 439
PspXI VCTCGAGB 1 cut(s) 373
PstI CTGCAG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 48
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
RseI CAYNNNNRTG 1 cut(s) 327
SaqAI TTAA 2 cut(s) 243, 564
SatI GCNGC 7 cut(s) 132, 234, 303, 306, 396, 407, 551
Sau3AI GATC 2 cut(s) 48, 78
SchI GAGTC 3 cut(s) 313, 365, 439
SfcI CTRYAG 1 cut(s) 303
Sfr274I CTCGAG 1 cut(s) 373
SlaI CTCGAG 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 4 cut(s) 269, 480, 545, 554
SspMI CTAG 2 cut(s) 183, 569
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 1 cut(s) 117
TaqI TCGA 1 cut(s) 374
TasI AATT 4 cut(s) 269, 480, 545, 554
Tru1I TTAA 2 cut(s) 243, 564
Tru9I TTAA 2 cut(s) 243, 564
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 7 cut(s) 131, 233, 302, 305, 395, 406, 550
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 4 cut(s) 32, 312, 519, 588
TspRI CASTG 1 cut(s) 180
XhoI CTCGAG 1 cut(s) 373
XspI CTAG 2 cut(s) 183, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.