FvH4_6g12281

Heme-binding-like protein At3g10130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016231
Physical Location & Seq
Reverse (-)
7407343 .. 7407945
603 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g12281.t1

Sequence Viewer

Length: 603 bp
ATGGGCATGGTATTTGGAAAAATTTGTGTAGAAACCCCGAAATACCAGGTCCTTAAATCCACAGCTGATTATGAAATCCGGCAGTATCCACCATCAGTGATAGCTCAGGTGACCTATGATCCATCAGTGTTCAAGGGCGACAAAGACGGTGGCTTCTCTGTGCTGGCCAATTACATCGGCGCTTTTGGCAAGCCCCAGAACACCAAGCCTGAGAAGATAGCCATGACAGCTCCAGTCATAACCCAACAGAGCACAGCTGAGAAGATCGCCATGACAGCTCCTGTTGTGACCAAAGAAGCCGGAGACGCGAAGACGGTGACAATGCAATTCCTGCTGCCTGCTAAGTATCAGAAGGCAGAGGAGGCGCCAAAGCCGGTGGATGAGAGAGTGGTGATTAGGGAAGAGGGTGGGAGGAAGTATGGGGTGGTGACTTTTGGAGGATCGGCGTCGGAGCAGGTGGTGGAGGATAGGGTGGAGAAACTGAAGAGGGCTTTGGAGAAAGATGGGTTTAAGATTACAGGGGAGTACTTGCTGGCAAGGTTTAACCCACCTTGGACTTTGCCTGCATTTAGGACTAATGAGGTTATGATCCCAGTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

201

Amino Acids

22.08

Weight (kDa)

8.48

Isoelectric Point (pI)

25.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 10 - 198 6.9e-47 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37970
fragaria_vesca FvH4_6g12281
malus_domestica MD04G1144100.v1.1 MD12G1158200.v1.1
prunus_persica Prupe.6G268400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0464101
rosa_laevigata RLG00000024723
rosa_multiflora Rmu_sc0006492.1_g000021 Rmu_sc0010401.1_g000002
rosa_roxburghii Rroxscaffold_6G00416000
rosa_rugosa Rorug03G0070700
rosa_samantha Rh3BG131900 Rh3CG134100 Rh3DG133100
rosa_wichuraiana Rw3G010790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 445
Acc36I ACCTGC 1 cut(s) 445
AccB1I GGYRCC 1 cut(s) 364
AccII CGCG 1 cut(s) 308
AclWI GGATC 3 cut(s) 113, 448, 583
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 503
AcyI GRCGYC 2 cut(s) 365, 446
AfaI GTAC 1 cut(s) 527
AgsI TTSAA 1 cut(s) 133
AjnI CCWGG 1 cut(s) 45
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AluBI AGCT 5 cut(s) 65, 104, 230, 257, 278
AluI AGCT 5 cut(s) 65, 104, 230, 257, 278
Alw21I GWGCWC 1 cut(s) 254
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 3 cut(s) 113, 448, 583
AlwNI CAGNNNCTG 1 cut(s) 281
AoxI GGCC 1 cut(s) 165
ApeKI GCWGC 1 cut(s) 334
ApoI RAATTY 1 cut(s) 21
AspLEI GCGC 2 cut(s) 182, 367
AspS9I GGNCC 1 cut(s) 49
AsuHPI GGTGA 4 cut(s) 121, 328, 403, 439
AvaII GGWCC 1 cut(s) 49
BalI TGGCCA 1 cut(s) 167
BanI GGYRCC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 254
BbvI GCAGC 1 cut(s) 321
BccI CCATC 3 cut(s) 100, 130, 497
BciT130I CCWGG 1 cut(s) 47
BciVI GTATCC 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 297
BfoI RGCGCY 2 cut(s) 183, 368
BfuAI ACCTGC 1 cut(s) 445
BfuI GTATCC 1 cut(s) 96
BisI GCNGC 1 cut(s) 335
BlsI GCNGC 1 cut(s) 336
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 1 cut(s) 47
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 1 cut(s) 366
BmrFI CCNGG 1 cut(s) 47
BmrI ACTGGG 1 cut(s) 587
BmuI ACTGGG 1 cut(s) 587
BpiI GAAGAC 1 cut(s) 317
BpmI CTGGAG 1 cut(s) 216
Bpu10I CCTNAGC 1 cut(s) 105
BsaHI GRCGYC 2 cut(s) 365, 446
BsaJI CCNNGG 1 cut(s) 551
Bse118I RCCGGY 1 cut(s) 373
Bse1I ACTGG 2 cut(s) 233, 593
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 551
BseGI GGATG 1 cut(s) 385
BseMII CTCAG 3 cut(s) 119, 201, 249
BseNI ACTGG 2 cut(s) 233, 593
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 1 cut(s) 167
BshNI GGYRCC 1 cut(s) 364
BsiHKAI GWGCWC 1 cut(s) 254
BsiSI CCGG 3 cut(s) 79, 300, 374
BsmAI GTCTC 1 cut(s) 297
BsmBI CGTCTC 1 cut(s) 297
BsnI GGCC 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 254
Bsp143I GATC 4 cut(s) 118, 264, 440, 588
BspANI GGCC 1 cut(s) 167
BspCNI CTCAG 3 cut(s) 118, 202, 250
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 1 cut(s) 366
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 3 cut(s) 113, 448, 583
BspT107I GGYRCC 1 cut(s) 364
BsrFI RCCGGY 1 cut(s) 373
BsrI ACTGG 2 cut(s) 233, 593
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 551
BssMI GATC 4 cut(s) 118, 264, 440, 588
BssNI GRCGYC 2 cut(s) 365, 446
BssT1I CCWWGG 1 cut(s) 551
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 2 cut(s) 149, 316
Bst6I CTCTTC 2 cut(s) 396, 479
BstACI GRCGYC 2 cut(s) 365, 446
BstAPI GCANNNNNTGC 1 cut(s) 331
BstC8I GCNNGC 5 cut(s) 165, 191, 339, 534, 564
BstDEI CTNAG 4 cut(s) 105, 210, 258, 342
BstEII GGTNACC 1 cut(s) 109
BstF5I GGATG 1 cut(s) 385
BstFNI CGCG 1 cut(s) 308
BstH2I RGCGCY 2 cut(s) 183, 368
BstHHI GCGC 2 cut(s) 182, 367
BstKTI GATC 4 cut(s) 121, 267, 443, 591
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 4 cut(s) 118, 264, 440, 588
BstMWI GCNNNNNNNGC 6 cut(s) 186, 227, 275, 305, 331, 362
BstNI CCWGG 1 cut(s) 47
BstPI GGTNACC 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 45
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 1 cut(s) 321
BstV2I GAAGAC 1 cut(s) 317
BsuI GTATCC 1 cut(s) 96
BsuRI GGCC 1 cut(s) 167
BtsCI GGATG 1 cut(s) 385
BtsIMutI CAGTG 2 cut(s) 102, 132
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 5 cut(s) 165, 191, 339, 534, 564
CaiI CAGNNNCTG 1 cut(s) 281
CfoI GCGC 2 cut(s) 182, 367
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 1 cut(s) 49
CseI GACGC 2 cut(s) 314, 435
CsiI ACCWGGT 1 cut(s) 45
Csp6I GTAC 1 cut(s) 526
CspCI CAANNNNNGTGG 4 cut(s) 130, 165, 357, 392
CviAII CATG 3 cut(s) 7, 223, 271
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 4 cut(s) 105, 210, 258, 342
DinI GGCGCC 1 cut(s) 366
DpnI GATC 4 cut(s) 120, 266, 442, 590
DpnII GATC 4 cut(s) 118, 264, 440, 588
EaeI YGGCCR 1 cut(s) 165
Eam1104I CTCTTC 2 cut(s) 396, 479
EarI CTCTTC 2 cut(s) 396, 479
Eco130I CCWWGG 1 cut(s) 551
Eco47I GGWCC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 503
Eco91I GGTNACC 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 49
EcoO65I GGTNACC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 551
EgeI GGCGCC 1 cut(s) 366
EheI GGCGCC 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 551
Esp3I CGTCTC 1 cut(s) 297
FaeI CATG 3 cut(s) 10, 226, 274
FaiI YATR 8 cut(s) 8, 72, 117, 224, 239, 272, 420, 587
FatI CATG 3 cut(s) 6, 222, 270
Fnu4HI GCNGC 1 cut(s) 335
FokI GGATG 1 cut(s) 392
Fsp4HI GCNGC 1 cut(s) 335
GlaI GCGC 2 cut(s) 181, 366
GluI GCNGC 1 cut(s) 335
GsuI CTGGAG 1 cut(s) 216
HaeII RGCGCY 2 cut(s) 183, 368
HaeIII GGCC 1 cut(s) 167
HapII CCGG 3 cut(s) 79, 300, 374
HgaI GACGC 2 cut(s) 314, 435
HhaI GCGC 2 cut(s) 182, 367
Hin1I GRCGYC 2 cut(s) 365, 446
Hin1II CATG 3 cut(s) 10, 226, 274
Hin6I GCGC 2 cut(s) 180, 365
HinP1I GCGC 2 cut(s) 180, 365
HpaII CCGG 3 cut(s) 79, 300, 374
HphI GGTGA 4 cut(s) 121, 328, 403, 439
Hpy188I TCNGA 2 cut(s) 351, 451
Hpy99I CGWCG 1 cut(s) 451
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 2 cut(s) 149, 316
HpyCH4V TGCA 2 cut(s) 325, 566
HpyF10VI GCNNNNNNNGC 6 cut(s) 186, 227, 275, 305, 331, 362
HpyF3I CTNAG 4 cut(s) 105, 210, 258, 342
Hsp92I GRCGYC 2 cut(s) 365, 446
Hsp92II CATG 3 cut(s) 10, 226, 274
HspAI GCGC 2 cut(s) 180, 365
KasI GGCGCC 1 cut(s) 364
Kzo9I GATC 4 cut(s) 118, 264, 440, 588
LmnI GCTCC 3 cut(s) 235, 283, 451
Lsp1109I GCAGC 1 cut(s) 321
MabI ACCWGGT 1 cut(s) 45
MaeIII GTNAC 4 cut(s) 109, 286, 316, 427
MalI GATC 4 cut(s) 120, 266, 442, 590
MboI GATC 4 cut(s) 118, 264, 440, 588
MboII GAAGA 5 cut(s) 226, 274, 322, 413, 496
MhlI GDGCHC 1 cut(s) 254
MlsI TGGCCA 1 cut(s) 167
MluCI AATT 3 cut(s) 21, 169, 326
MluNI TGGCCA 1 cut(s) 167
Mly113I GGCGCC 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 429
MnlI CCTC 8 cut(s) 352, 355, 397, 405, 431, 457, 480, 574
Mox20I TGGCCA 1 cut(s) 167
MscI TGGCCA 1 cut(s) 167
MseI TTAA 3 cut(s) 54, 510, 543
Msp20I TGGCCA 1 cut(s) 167
MspA1I CMGCKG 2 cut(s) 65, 257
MspI CCGG 3 cut(s) 79, 300, 374
MspR9I CCNGG 1 cut(s) 47
MvaI CCWGG 1 cut(s) 47
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 6 cut(s) 186, 227, 275, 305, 331, 362
NarI GGCGCC 1 cut(s) 365
NdeII GATC 4 cut(s) 118, 264, 440, 588
NlaIII CATG 3 cut(s) 10, 226, 274
NlaIV GGNNCC 1 cut(s) 366
NmuCI GTSAC 4 cut(s) 109, 286, 316, 427
PaqCI CACCTGC 1 cut(s) 445
PkrI GCNGC 1 cut(s) 336
PluTI GGCGCC 1 cut(s) 368
PpuMI RGGWCCY 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 49
Psp6I CCWGG 1 cut(s) 45
PspEI GGTNACC 1 cut(s) 109
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 366
PspPI GGNCC 1 cut(s) 49
PspPPI RGGWCCY 1 cut(s) 49
PstNI CAGNNNCTG 1 cut(s) 281
PvuII CAGCTG 2 cut(s) 65, 257
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
SaqAI TTAA 3 cut(s) 54, 510, 543
SatI GCNGC 1 cut(s) 335
Sau3AI GATC 4 cut(s) 118, 264, 440, 588
Sau96I GGNCC 1 cut(s) 49
ScaI AGTACT 1 cut(s) 527
ScrFI CCNGG 1 cut(s) 47
SduI GDGCHC 1 cut(s) 254
SexAI ACCWGGT 1 cut(s) 45
SfoI GGCGCC 1 cut(s) 366
SinI GGWCC 1 cut(s) 49
Sse9I AATT 3 cut(s) 21, 169, 326
SspDI GGCGCC 1 cut(s) 364
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 1 cut(s) 551
TaaI ACNGT 2 cut(s) 149, 316
TasI AATT 3 cut(s) 21, 169, 326
TatI WGTACW 1 cut(s) 525
Tru1I TTAA 3 cut(s) 54, 510, 543
Tru9I TTAA 3 cut(s) 54, 510, 543
TscAI CASTG 2 cut(s) 102, 132
TseFI GTSAC 4 cut(s) 109, 286, 316, 427
TseI GCWGC 1 cut(s) 334
Tsp45I GTSAC 4 cut(s) 109, 286, 316, 427
TspDTI ATGAA 1 cut(s) 87
TspRI CASTG 2 cut(s) 102, 132
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 1 cut(s) 21
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.