Rh3CG134100

Heme-binding-like protein At3g10130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0016231
Physical Location & Seq
Reverse (-)
10966930 .. 10967982
1053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG134100.1

Sequence Viewer

Length: 1053 bp
ATGAGATTATTTTTTTTCAATTACGATGGAGGATGGACAAAGAAGACGGCGAATGGAGCCATTTGCAATGAAGAGGATGTAAAACTCGTTATCTCCAAATGGAGACTCAAAAGGAGAGGAGGAAGTTTGGGAGAGCAAAATCCTACTGCGGTTAGGGAAGGTCGGGTACTGTTCCATTCGGATCCAGAAGGCCCAGAAATGGGCCCATCAAAATGGTGGCCGGACAGTGTCCACGAGTACATCACCAAAATCAGCCACTCGTTCAAGAGTGGACATTTCCAACCTCTTCCACAAGCAATCCCATCAAACGAAAGCCAAAAACAAGAGAAAACCTCCTCTCCAAGCAAAGGATCAATACAAATAGGTTTCTCCCAACAATCCACAGCAAATTCCATCCTCATTCTCTCAAATTTTCACAAGCAAATCACAAACAGAACCCCAAATTCCAACATGGGAATGGTATTTGGAAAAATTGCTGTAGAAACCCCAAAATACCAAGTCCTTAAATCCACAGCCGATTACGAAATCCGGCAATATGCACCATCAGTGGCAGCTCAAGTCACCTATGATCCATCAGTGTTCAATGGCAACAGAGATGGTGGGTTTACTGTGCTGGCCAATTACATAGGCGCTCTTGGCAATCCCCAGAACACCAAGCCTGAGAAGATAGCCATGACTGCTCCAGTCATAACCCAGCAAAGCTCGGCTGAAAAGATCGCCATGACAGCTCCAGTTGTGACGAAAGAAGCTGGGGATGCGAAGACGGTGACAATGCAATTCCTGCTGCCTGCTAAGTATCAGAAGGCGGAGGAGGCGCCGAAGCCGGTGGATGAGAGAGTGGTGATTAGGGAAGTGGGGGAGAGGAAGTATGCGGTGGTGACTTTTAGCGGAGTGGCGTCAGAGCAAGTGGTGGAGGATCGGGTGGAGAAGCTTAAGAGGGCTTTGGAGAAAGATGGGTTTAAGATTATAGGGGAGTATTTGCTGGCGAGGTTTAACCCACCTTGGACCCTGCCTGCATTTAGGACAAATGAGGTTATGATCCCAGTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

350

Amino Acids

38.91

Weight (kDa)

9.22

Isoelectric Point (pI)

34.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 160 - 234 3.1e-17 SOUL heme-binding protein
SOUL PF04832 234 - 348 7e-31 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37970
fragaria_vesca FvH4_6g12281
malus_domestica MD04G1144100.v1.1 MD12G1158200.v1.1
prunus_persica Prupe.6G268400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0464101
rosa_laevigata RLG00000024723
rosa_multiflora Rmu_sc0006492.1_g000021 Rmu_sc0010401.1_g000002
rosa_roxburghii Rroxscaffold_6G00416000
rosa_rugosa Rorug03G0070700
rosa_samantha Rh3BG131900 Rh3CG134100 Rh3DG133100
rosa_wichuraiana Rw3G010790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 814
AciI CCGC 4 cut(s) 149, 806, 872, 888
AclWI GGATC 6 cut(s) 176, 189, 358, 563, 924, 1033
AcoI YGGCCR 2 cut(s) 218, 615
AcsI RAATTY 3 cut(s) 388, 409, 442
AcyI GRCGYC 2 cut(s) 815, 896
AfaI GTAC 2 cut(s) 168, 239
AfiI CCNNNNNNNGG 3 cut(s) 199, 200, 347
AflII CTTAAG 1 cut(s) 932
AgsI TTSAA 3 cut(s) 19, 265, 583
AluBI AGCT 5 cut(s) 554, 702, 728, 749, 931
AluI AGCT 5 cut(s) 554, 702, 728, 749, 931
Alw26I GTCTC 1 cut(s) 97
AlwI GGATC 6 cut(s) 176, 189, 358, 563, 924, 1033
AoxI GGCC 4 cut(s) 190, 202, 218, 615
ApaI GGGCCC 1 cut(s) 206
ApeKI GCWGC 2 cut(s) 551, 784
ApoI RAATTY 3 cut(s) 388, 409, 442
AspLEI GCGC 2 cut(s) 632, 817
AspS9I GGNCC 4 cut(s) 191, 202, 203, 1005
AsuHPI GGTGA 5 cut(s) 235, 553, 778, 853, 889
AvaII GGWCC 1 cut(s) 1005
BaeGI GKGCMC 1 cut(s) 206
BalI TGGCCA 1 cut(s) 617
BamHI GGATCC 1 cut(s) 181
BanI GGYRCC 1 cut(s) 814
BanII GRGCYC 1 cut(s) 206
BarI GAAGNNNNNNTAC 2 cut(s) 150, 182
BauI CACGAG 1 cut(s) 233
BbsI GAAGAC 2 cut(s) 50, 767
BbvI GCAGC 2 cut(s) 563, 771
BccI CCATC 9 cut(s) 20, 27, 214, 310, 401, 550, 580, 590, 947
BceAI ACGGC 1 cut(s) 63
BcoDI GTCTC 1 cut(s) 97
BfmI CTRYAG 1 cut(s) 477
BfoI RGCGCY 2 cut(s) 633, 818
BfrI CTTAAG 1 cut(s) 932
BisI GCNGC 2 cut(s) 552, 785
BlsI GCNGC 2 cut(s) 553, 786
Bme18I GGWCC 1 cut(s) 1005
BmgT120I GGNCC 4 cut(s) 191, 202, 203, 1005
BmiI GGNNCC 5 cut(s) 58, 183, 204, 816, 1007
BmrI ACTGGG 1 cut(s) 1037
BmsI GCATC 1 cut(s) 745
BmuI ACTGGG 1 cut(s) 1037
BpiI GAAGAC 2 cut(s) 50, 767
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 2 cut(s) 666, 714
BpuEI CTTGAG 1 cut(s) 540
BsaHI GRCGYC 2 cut(s) 815, 896
BsaJI CCNNGG 1 cut(s) 1001
BsaXI ACNNNNNCTCC 4 cut(s) 322, 352, 851, 881
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 200, 347
Bse118I RCCGGY 1 cut(s) 823
Bse1I ACTGG 3 cut(s) 683, 731, 1043
Bse3DI GCAATG 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 1001
BseGI GGATG 5 cut(s) 38, 82, 393, 760, 835
BseLI CCNNNNNNNGG 3 cut(s) 199, 200, 347
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 651
BseNI ACTGG 3 cut(s) 683, 731, 1043
BseRI GAGGAG 3 cut(s) 132, 325, 824
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 2 cut(s) 563, 771
BseYI CCCAGC 2 cut(s) 693, 749
BshFI GGCC 4 cut(s) 192, 204, 220, 617
BshNI GGYRCC 1 cut(s) 814
BsiSI CCGG 3 cut(s) 221, 529, 824
BslI CCNNNNNNNGG 3 cut(s) 199, 200, 347
BsmAI GTCTC 1 cut(s) 97
BsnI GGCC 4 cut(s) 192, 204, 220, 617
Bsp120I GGGCCC 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 6 cut(s) 181, 350, 568, 714, 916, 1038
BspACI CCGC 4 cut(s) 149, 806, 872, 888
BspANI GGCC 4 cut(s) 192, 204, 220, 617
BspCNI CTCAG 1 cut(s) 652
BspLI GGNNCC 5 cut(s) 58, 183, 204, 816, 1007
BspPI GGATC 6 cut(s) 176, 189, 358, 563, 924, 1033
BspT107I GGYRCC 1 cut(s) 814
BspTI CTTAAG 1 cut(s) 932
BsrDI GCAATG 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 823
BsrI ACTGG 3 cut(s) 683, 731, 1043
BssAI RCCGGY 1 cut(s) 823
BssECI CCNNGG 1 cut(s) 1001
BssMI GATC 6 cut(s) 181, 350, 568, 714, 916, 1038
BssNI GRCGYC 2 cut(s) 815, 896
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 1001
Bst2BI CACGAG 1 cut(s) 233
Bst4CI ACNGT 4 cut(s) 171, 227, 610, 766
Bst6I CTCTTC 2 cut(s) 66, 291
BstACI GRCGYC 2 cut(s) 815, 896
BstAFI CTTAAG 1 cut(s) 932
BstAPI GCANNNNNTGC 1 cut(s) 781
BstC8I GCNNGC 4 cut(s) 615, 789, 984, 1014
BstDEI CTNAG 2 cut(s) 660, 792
BstF5I GGATG 5 cut(s) 38, 82, 393, 760, 835
BstH2I RGCGCY 2 cut(s) 633, 818
BstHHI GCGC 2 cut(s) 632, 817
BstKTI GATC 6 cut(s) 184, 353, 571, 717, 919, 1041
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 6 cut(s) 181, 350, 568, 714, 916, 1038
BstMWI GCNNNNNNNGC 7 cut(s) 56, 636, 677, 725, 755, 781, 812
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 1 cut(s) 206
BstV1I GCAGC 2 cut(s) 563, 771
BstV2I GAAGAC 2 cut(s) 50, 767
BstX2I RGATCY 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 213
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 4 cut(s) 192, 204, 220, 617
BtsCI GGATG 5 cut(s) 38, 82, 393, 760, 835
BtsIMutI CAGTG 3 cut(s) 232, 552, 582
Cac8I GCNNGC 4 cut(s) 615, 789, 984, 1014
CfoI GCGC 2 cut(s) 632, 817
Cfr10I RCCGGY 1 cut(s) 823
Cfr13I GGNCC 4 cut(s) 191, 202, 203, 1005
CseI GACGC 1 cut(s) 885
Csp6I GTAC 2 cut(s) 167, 238
CviAII CATG 3 cut(s) 451, 673, 721
CviQI GTAC 2 cut(s) 167, 238
DdeI CTNAG 2 cut(s) 660, 792
DinI GGCGCC 1 cut(s) 816
DpnI GATC 6 cut(s) 183, 352, 570, 716, 918, 1040
DpnII GATC 6 cut(s) 181, 350, 568, 714, 916, 1038
EaeI YGGCCR 2 cut(s) 218, 615
Eam1104I CTCTTC 2 cut(s) 66, 291
EarI CTCTTC 2 cut(s) 66, 291
EciI GGCGGA 1 cut(s) 821
Eco130I CCWWGG 1 cut(s) 1001
Eco24I GRGCYC 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 1005
EcoT14I CCWWGG 1 cut(s) 1001
EcoT38I GRGCYC 1 cut(s) 206
EgeI GGCGCC 1 cut(s) 816
EheI GGCGCC 1 cut(s) 816
ErhI CCWWGG 1 cut(s) 1001
FaeI CATG 3 cut(s) 454, 676, 724
FatI CATG 3 cut(s) 450, 672, 720
Fnu4HI GCNGC 2 cut(s) 552, 785
FokI GGATG 5 cut(s) 45, 89, 380, 767, 842
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 2 cut(s) 552, 785
GlaI GCGC 2 cut(s) 631, 816
GluI GCNGC 2 cut(s) 552, 785
GsaI CCCAGC 2 cut(s) 697, 753
GsuI CTGGAG 2 cut(s) 666, 714
HaeII RGCGCY 2 cut(s) 633, 818
HaeIII GGCC 4 cut(s) 192, 204, 220, 617
HapII CCGG 3 cut(s) 221, 529, 824
HgaI GACGC 1 cut(s) 885
HhaI GCGC 2 cut(s) 632, 817
Hin1I GRCGYC 2 cut(s) 815, 896
Hin1II CATG 3 cut(s) 454, 676, 724
Hin6I GCGC 2 cut(s) 630, 815
HinP1I GCGC 2 cut(s) 630, 815
HindIII AAGCTT 1 cut(s) 929
HinfI GANTC 1 cut(s) 105
HpaII CCGG 3 cut(s) 221, 529, 824
HphI GGTGA 5 cut(s) 235, 553, 778, 853, 889
Hpy166II GTNNAC 3 cut(s) 232, 272, 606
Hpy188I TCNGA 3 cut(s) 181, 801, 901
Hpy188III TCNNGA 2 cut(s) 185, 265
Hpy8I GTNNAC 3 cut(s) 232, 272, 606
HpyAV CCTTC 3 cut(s) 152, 182, 796
HpyCH4III ACNGT 4 cut(s) 171, 227, 610, 766
HpyCH4V TGCA 4 cut(s) 66, 539, 775, 1016
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 636, 677, 725, 755, 781, 812
HpyF3I CTNAG 2 cut(s) 660, 792
Hsp92I GRCGYC 2 cut(s) 815, 896
Hsp92II CATG 3 cut(s) 454, 676, 724
HspAI GCGC 2 cut(s) 630, 815
KasI GGCGCC 1 cut(s) 814
Kzo9I GATC 6 cut(s) 181, 350, 568, 714, 916, 1038
LmnI GCTCC 3 cut(s) 56, 685, 733
Lsp1109I GCAGC 2 cut(s) 563, 771
LweI GCATC 1 cut(s) 745
MaeIII GTNAC 4 cut(s) 559, 736, 766, 877
MalI GATC 6 cut(s) 183, 352, 570, 716, 918, 1040
MboI GATC 6 cut(s) 181, 350, 568, 714, 916, 1038
MboII GAAGA 5 cut(s) 55, 83, 278, 676, 772
MflI RGATCY 1 cut(s) 181
MhlI GDGCHC 1 cut(s) 206
MlsI TGGCCA 1 cut(s) 617
MluCI AATT 7 cut(s) 19, 388, 409, 442, 471, 619, 776
MluNI TGGCCA 1 cut(s) 617
Mly113I GGCGCC 1 cut(s) 815
MlyI GAGTC 1 cut(s) 99
MmeI TCCRAC 2 cut(s) 304, 471
Mox20I TGGCCA 1 cut(s) 617
MscI TGGCCA 1 cut(s) 617
MseI TTAA 4 cut(s) 504, 933, 960, 993
MslI CAYNNNNRTG 2 cut(s) 211, 455
Msp20I TGGCCA 1 cut(s) 617
MspCI CTTAAG 1 cut(s) 932
MspI CCGG 3 cut(s) 221, 529, 824
MwoI GCNNNNNNNGC 7 cut(s) 56, 636, 677, 725, 755, 781, 812
NarI GGCGCC 1 cut(s) 815
NdeII GATC 6 cut(s) 181, 350, 568, 714, 916, 1038
NlaIII CATG 3 cut(s) 454, 676, 724
NlaIV GGNNCC 5 cut(s) 58, 183, 204, 816, 1007
NmeAIII GCCGAG 1 cut(s) 683
NmuCI GTSAC 4 cut(s) 559, 736, 766, 877
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 553, 786
PleI GAGTC 1 cut(s) 99
PluTI GGCGCC 1 cut(s) 818
PpsI GAGTC 1 cut(s) 99
PspFI CCCAGC 2 cut(s) 693, 749
PspN4I GGNNCC 5 cut(s) 58, 183, 204, 816, 1007
PspOMI GGGCCC 1 cut(s) 202
PspPI GGNCC 4 cut(s) 191, 202, 203, 1005
PsuI RGATCY 1 cut(s) 181
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 2 cut(s) 168, 239
RsaNI GTAC 2 cut(s) 167, 238
RseI CAYNNNNRTG 2 cut(s) 211, 455
SaqAI TTAA 4 cut(s) 504, 933, 960, 993
SatI GCNGC 2 cut(s) 552, 785
Sau3AI GATC 6 cut(s) 181, 350, 568, 714, 916, 1038
Sau96I GGNCC 4 cut(s) 191, 202, 203, 1005
SchI GAGTC 1 cut(s) 99
SduI GDGCHC 1 cut(s) 206
SfaNI GCATC 1 cut(s) 745
SfcI CTRYAG 1 cut(s) 477
SfoI GGCGCC 1 cut(s) 816
SinI GGWCC 1 cut(s) 1005
SmiMI CAYNNNNRTG 2 cut(s) 211, 455
SmlI CTYRAG 2 cut(s) 555, 932
SmoI CTYRAG 2 cut(s) 555, 932
Sse9I AATT 7 cut(s) 19, 388, 409, 442, 471, 619, 776
SsiI CCGC 4 cut(s) 149, 806, 872, 888
SspDI GGCGCC 1 cut(s) 814
StyI CCWWGG 1 cut(s) 1001
TaaI ACNGT 4 cut(s) 171, 227, 610, 766
TasI AATT 7 cut(s) 19, 388, 409, 442, 471, 619, 776
TatI WGTACW 1 cut(s) 237
Tru1I TTAA 4 cut(s) 504, 933, 960, 993
Tru9I TTAA 4 cut(s) 504, 933, 960, 993
TscAI CASTG 3 cut(s) 232, 552, 582
TseFI GTSAC 4 cut(s) 559, 736, 766, 877
TseI GCWGC 2 cut(s) 551, 784
Tsp45I GTSAC 4 cut(s) 559, 736, 766, 877
TspDTI ATGAA 1 cut(s) 84
TspRI CASTG 3 cut(s) 232, 552, 582
Tth111I GACNNNGTC 1 cut(s) 227
Vha464I CTTAAG 1 cut(s) 932
VpaK11BI GGWCC 1 cut(s) 1005
XapI RAATTY 3 cut(s) 388, 409, 442
XcmI CCANNNNNNNNNTGG 2 cut(s) 213, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.