MD12G1158200.v1.1

Heme-binding-like protein At3g10130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016231
Physical Location & Seq
Forward (+)
23884351 .. 23884959
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1158200.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGGCATGGTTTTTGGAAAGATATGCGTGGAAACCCCAAAATACAAAGTCCTCCACTCCACCGCCCTCTACGAAATCCGGCAATACCCGCCTTCAGTAGTAGCCCAGTTCACCTACGATCCGTCAGACCTCAAGGGCGACAAAGATGGCGGCTTTGCCATTCTGGCCAATTACATCGGCGCGTTGGGAAACCCCCAGAACACCAAACCCGAGAAAATCGCCATGACGGCTCCGGTCATAACCAAATCCCAAAACCAAGAAAGCGAAATGATCGCCATGACGGCTCCGGTCGTGACGAGGGAGGAGAAGGGGAGGAAGATGGTGACGATGGAGTTTTTGCTGCCGGCGAAGTATGGGAAGGCGGAGGAGGCGCCGAAGCCGGTGGACGAGAGGGTGGTGATTACGGAGGAGGGAGAGAGGAAGTATGGGGTGGTGGGATTCACTGGAGTGGCGACGGAGAAGGTGGTGGCGGAGAAGGTGGAGAAGTTGAAGGAGAGGTTGGAGAAAGATGGGTATAAGATTATTGGGGATCATCTGCTGGCTAGGTATAATCCGCCATGGACGTTGCCTCCGTTCAGGACCAATGAGGTTCTGATTCCGATTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

203

Amino Acids

22.55

Weight (kDa)

6.78

Isoelectric Point (pI)

29.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 10 - 85 7.2e-17 SOUL heme-binding protein
SOUL PF04832 83 - 200 3.3e-29 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37970
fragaria_vesca FvH4_6g12281
malus_domestica MD04G1144100.v1.1 MD12G1158200.v1.1
prunus_persica Prupe.6G268400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0464101
rosa_laevigata RLG00000024723
rosa_multiflora Rmu_sc0006492.1_g000021 Rmu_sc0010401.1_g000002
rosa_roxburghii Rroxscaffold_6G00416000
rosa_rugosa Rorug03G0070700
rosa_samantha Rh3BG131900 Rh3CG134100 Rh3DG133100
rosa_wichuraiana Rw3G010790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 370
AccII CGCG 1 cut(s) 182
AciI CCGC 6 cut(s) 63, 89, 150, 362, 470, 554
AclWI GGATC 2 cut(s) 113, 537
AcoI YGGCCR 1 cut(s) 165
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 371
AgsI TTSAA 1 cut(s) 490
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AleI CACNNNNGTG 1 cut(s) 446
AlwI GGATC 2 cut(s) 113, 537
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 1 cut(s) 165
ApeKI GCWGC 1 cut(s) 340
AspLEI GCGC 2 cut(s) 182, 373
AspS9I GGNCC 1 cut(s) 579
AsuHPI GGTGA 3 cut(s) 103, 334, 409
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 579
BalI TGGCCA 1 cut(s) 167
BanI GGYRCC 1 cut(s) 370
BbvI GCAGC 1 cut(s) 327
BccI CCATC 4 cut(s) 140, 313, 322, 503
BceAI ACGGC 2 cut(s) 243, 297
BfaI CTAG 1 cut(s) 543
BfoI RGCGCY 1 cut(s) 374
BglI GCCNNNNNGGC 3 cut(s) 164, 227, 281
BisI GCNGC 2 cut(s) 151, 341
BlsI GCNGC 2 cut(s) 152, 342
Bme18I GGWCC 1 cut(s) 579
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 579
BmiI GGNNCC 3 cut(s) 231, 285, 372
BmrI ACTGGG 1 cut(s) 100
BmuI ACTGGG 1 cut(s) 100
BpmI CTGGAG 1 cut(s) 465
BpuEI CTTGAG 1 cut(s) 116
BsaHI GRCGYC 1 cut(s) 371
BsaJI CCNNGG 1 cut(s) 557
BsaWI WCCGGW 2 cut(s) 232, 286
BsaXI ACNNNNNCTCC 2 cut(s) 553, 583
Bse118I RCCGGY 2 cut(s) 343, 379
Bse1I ACTGG 2 cut(s) 106, 448
BseDI CCNNGG 1 cut(s) 557
BseNI ACTGG 2 cut(s) 106, 448
BseRI GAGGAG 3 cut(s) 317, 380, 422
BseXI GCAGC 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 182
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 1 cut(s) 167
BshNI GGYRCC 1 cut(s) 370
BsiEI CGRYCG 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 5 cut(s) 79, 233, 287, 344, 380
BsnI GGCC 1 cut(s) 167
BsoBI CYCGRG 1 cut(s) 209
Bsp143I GATC 3 cut(s) 118, 270, 529
Bsp19I CCATGG 1 cut(s) 557
BspACI CCGC 6 cut(s) 63, 89, 150, 362, 470, 554
BspANI GGCC 1 cut(s) 167
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 3 cut(s) 231, 285, 372
BspPI GGATC 2 cut(s) 113, 537
BspT107I GGYRCC 1 cut(s) 370
BsrFI RCCGGY 2 cut(s) 343, 379
BsrI ACTGG 2 cut(s) 106, 448
BssAI RCCGGY 2 cut(s) 343, 379
BssECI CCNNGG 1 cut(s) 557
BssMI GATC 3 cut(s) 118, 270, 529
BssNI GRCGYC 1 cut(s) 371
BssT1I CCWWGG 1 cut(s) 557
BstACI GRCGYC 1 cut(s) 371
BstC8I GCNNGC 2 cut(s) 345, 540
BstDSI CCRYGG 1 cut(s) 557
BstFNI CGCG 1 cut(s) 182
BstH2I RGCGCY 1 cut(s) 374
BstHHI GCGC 2 cut(s) 182, 373
BstKTI GATC 3 cut(s) 121, 273, 532
BstMBI GATC 3 cut(s) 118, 270, 529
BstMCI CGRYCG 1 cut(s) 291
BstMWI GCNNNNNNNGC 5 cut(s) 88, 164, 227, 281, 368
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 327
BsuRI GGCC 1 cut(s) 167
BtgI CCRYGG 1 cut(s) 557
BtsIMutI CAGTG 1 cut(s) 441
Cac8I GCNNGC 2 cut(s) 345, 540
CfoI GCGC 2 cut(s) 182, 373
Cfr10I RCCGGY 2 cut(s) 343, 379
Cfr13I GGNCC 1 cut(s) 579
CviAII CATG 4 cut(s) 7, 223, 277, 558
CviJI RGCY 7 cut(s) 104, 153, 167, 230, 284, 379, 542
CviKI_1 RGCY 7 cut(s) 104, 153, 167, 230, 284, 379, 542
DinI GGCGCC 1 cut(s) 372
DpnI GATC 3 cut(s) 120, 272, 531
DpnII GATC 3 cut(s) 118, 270, 529
EaeI YGGCCR 1 cut(s) 165
EciI GGCGGA 3 cut(s) 377, 485, 543
Eco130I CCWWGG 1 cut(s) 557
Eco47I GGWCC 1 cut(s) 579
Eco57I CTGAAG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 557
EgeI GGCGCC 1 cut(s) 372
EheI GGCGCC 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 557
FaeI CATG 4 cut(s) 10, 226, 280, 561
FatI CATG 4 cut(s) 6, 222, 276, 557
FauI CCCGC 1 cut(s) 96
Fnu4HI GCNGC 2 cut(s) 151, 341
Fsp4HI GCNGC 2 cut(s) 151, 341
FspBI CTAG 1 cut(s) 543
GlaI GCGC 2 cut(s) 181, 372
GluI GCNGC 2 cut(s) 151, 341
GsuI CTGGAG 1 cut(s) 465
HaeII RGCGCY 1 cut(s) 374
HaeIII GGCC 1 cut(s) 167
HapII CCGG 5 cut(s) 79, 233, 287, 344, 380
HhaI GCGC 2 cut(s) 182, 373
Hin1I GRCGYC 1 cut(s) 371
Hin1II CATG 4 cut(s) 10, 226, 280, 561
Hin6I GCGC 2 cut(s) 180, 371
HinP1I GCGC 2 cut(s) 180, 371
HinfI GANTC 2 cut(s) 438, 595
HpaII CCGG 5 cut(s) 79, 233, 287, 344, 380
HphI GGTGA 3 cut(s) 103, 334, 409
Hpy166II GTNNAC 2 cut(s) 111, 385
Hpy188I TCNGA 3 cut(s) 127, 594, 600
Hpy188III TCNNGA 2 cut(s) 292, 577
Hpy8I GTNNAC 2 cut(s) 111, 385
Hpy99I CGWCG 1 cut(s) 457
HpyAV CCTTC 6 cut(s) 102, 301, 352, 454, 469, 484
HpyCH4IV ACGT 1 cut(s) 563
HpyF10VI GCNNNNNNNGC 5 cut(s) 88, 164, 227, 281, 368
HpySE526I ACGT 1 cut(s) 563
Hsp92I GRCGYC 1 cut(s) 371
Hsp92II CATG 4 cut(s) 10, 226, 280, 561
HspAI GCGC 2 cut(s) 180, 371
KasI GGCGCC 1 cut(s) 370
KroI GCCGGC 1 cut(s) 343
KroNI GCCGGC 1 cut(s) 345
Kzo9I GATC 3 cut(s) 118, 270, 529
LmnI GCTCC 2 cut(s) 235, 289
Lsp1109I GCAGC 1 cut(s) 327
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 563
MaeIII GTNAC 2 cut(s) 292, 322
MalI GATC 3 cut(s) 120, 272, 531
MboI GATC 3 cut(s) 118, 270, 529
MboII GAAGA 1 cut(s) 328
MlsI TGGCCA 1 cut(s) 167
MluCI AATT 1 cut(s) 169
MluNI TGGCCA 1 cut(s) 167
Mly113I GGCGCC 1 cut(s) 371
MmeI TCCRAC 1 cut(s) 480
Mox20I TGGCCA 1 cut(s) 167
MroNI GCCGGC 1 cut(s) 343
MscI TGGCCA 1 cut(s) 167
MslI CAYNNNNRTG 1 cut(s) 446
Msp20I TGGCCA 1 cut(s) 167
MspI CCGG 5 cut(s) 79, 233, 287, 344, 380
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 5 cut(s) 88, 164, 227, 281, 368
NaeI GCCGGC 1 cut(s) 345
NarI GGCGCC 1 cut(s) 371
NcoI CCATGG 1 cut(s) 557
NdeII GATC 3 cut(s) 118, 270, 529
NgoMIV GCCGGC 1 cut(s) 343
NlaIII CATG 4 cut(s) 10, 226, 280, 561
NlaIV GGNNCC 3 cut(s) 231, 285, 372
NmuCI GTSAC 2 cut(s) 292, 322
OliI CACNNNNGTG 1 cut(s) 446
PcsI WCGNNNNNNNCGW 1 cut(s) 569
PdiI GCCGGC 1 cut(s) 345
PfeI GAWTC 2 cut(s) 438, 595
PkrI GCNGC 2 cut(s) 152, 342
PluTI GGCGCC 1 cut(s) 374
PspN4I GGNNCC 3 cut(s) 231, 285, 372
PspPI GGNCC 1 cut(s) 579
RseI CAYNNNNRTG 1 cut(s) 446
SatI GCNGC 2 cut(s) 151, 341
Sau3AI GATC 3 cut(s) 118, 270, 529
Sau96I GGNCC 1 cut(s) 579
SetI ASST 8 cut(s) 116, 132, 465, 480, 500, 548, 566, 591
SfoI GGCGCC 1 cut(s) 372
SinI GGWCC 1 cut(s) 579
SmiMI CAYNNNNRTG 1 cut(s) 446
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 1 cut(s) 169
SsiI CCGC 6 cut(s) 63, 89, 150, 362, 470, 554
SspDI GGCGCC 1 cut(s) 370
SspMI CTAG 1 cut(s) 543
StyI CCWWGG 1 cut(s) 557
TaiI ACGT 1 cut(s) 566
TasI AATT 1 cut(s) 169
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 438, 595
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 2 cut(s) 292, 322
TseI GCWGC 1 cut(s) 340
Tsp45I GTSAC 2 cut(s) 292, 322
TspGWI ACGGA 4 cut(s) 111, 419, 470, 561
TspRI CASTG 1 cut(s) 448
VpaK11BI GGWCC 1 cut(s) 579
XspI CTAG 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.