RLG00000024723

Heme-binding-like protein At3g10130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016231
Physical Location & Seq
Forward (+)
38541795 .. 38542727
933 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024723

Sequence Viewer

Length: 933 bp
ATGGAGACTCAGTTTCCTACTGCGGTTAGGGAAGGCTGGGTACTGTTCCATTCGGATCCAGAAGGCCCAAAAATAGGCCCATCGAAATGGTGGCCAGACAGTATCCACGAGTACATGACCGAAATCAGCCACTCGTTCAAGAGTATACATTTCCAAATTCCAACCTCTTCACAAGCAATCCCATCAAAAGAAAGAAAAAAAAGAGAGAGAACCTCCTCTCCAAGCAAAGGATCAATACAAATAGGTTTCTCCCAACAATCCACAGCAAATTCCATCCTCATTCTCTCAAGTTTTCAAAAGCAAATCACAAATAGAACCCCAAATTCCAACATGGGAATGGTATTTGGAAAAATTTGTGTAGAAACCCCAAAATACCAAGTCCTTAAATCCACAGCCGATTACGAAATCCGGCAGTATGCACCATCAGTGGCAGCTCAACTGACCTATGATCCATCAGTGTTCAATGGCAACAGAGATGGTGGGTTTACTGTGCTGGCCAATTACATAGGCGCCTTGGGGAATCCCCAAAACACCAAGCCTGAGAAGATAGCCATGACTGCTCCAGTCATAACCCAGCAAAGCTCGGCTGAAAAGATCGCCATGACAGCTCCAGTTGTGACGAAAGAAGCCGGGGACGCAAAGACGGTGACAATGCAATTCCTGCTGCCTGCTAAGTATCAGAAGGCGGAGGAGGCGCCGAAGCCGGTGGATGAGAGAGTGGTGATTAGGGAAGTGGGGGAGAGGAAGTATGGGGTGGTGACTTTTAGCGGAGTGGCGTCAGAGCAAGTGGTGGAGGATCGGGTGGAGAAGCTGAAGAGGGCTTTGGAGAAAGATGGGTTTAAGATTATAGGGGAGTATTTGCTGGCGAGGTATAACCCACCTTGGACTTTGCCTGCATTTAGGACTAATGAGATTATGATCCCAGTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

311

Amino Acids

34.58

Weight (kDa)

9.05

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 120 - 194 9.6e-17 SOUL heme-binding protein
SOUL PF04832 194 - 308 7.3e-31 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37970
fragaria_vesca FvH4_6g12281
malus_domestica MD04G1144100.v1.1 MD12G1158200.v1.1
prunus_persica Prupe.6G268400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0464101
rosa_laevigata RLG00000024723
rosa_multiflora Rmu_sc0006492.1_g000021 Rmu_sc0010401.1_g000002
rosa_roxburghii Rroxscaffold_6G00416000
rosa_rugosa Rorug03G0070700
rosa_samantha Rh3BG131900 Rh3CG134100 Rh3DG133100
rosa_wichuraiana Rw3G010790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 509, 694
AccI GTMKAC 1 cut(s) 145
AciI CCGC 3 cut(s) 23, 686, 768
AclWI GGATC 6 cut(s) 50, 63, 238, 443, 804, 913
AcoI YGGCCR 2 cut(s) 92, 495
AcsI RAATTY 4 cut(s) 156, 268, 322, 351
AcuI CTGAAG 1 cut(s) 833
AcyI GRCGYC 3 cut(s) 510, 695, 776
AfaI GTAC 2 cut(s) 42, 113
AfiI CCNNNNNNNGG 2 cut(s) 74, 227
AgsI TTSAA 3 cut(s) 139, 296, 463
AjuI GAANNNNNNNTTGG 2 cut(s) 806, 838
AloI GAACNNNNNNTCC 2 cut(s) 202, 234
AluBI AGCT 4 cut(s) 434, 582, 608, 811
AluI AGCT 4 cut(s) 434, 582, 608, 811
AlwI GGATC 6 cut(s) 50, 63, 238, 443, 804, 913
AoxI GGCC 4 cut(s) 64, 76, 92, 495
ApeKI GCWGC 2 cut(s) 431, 664
ApoI RAATTY 4 cut(s) 156, 268, 322, 351
AspLEI GCGC 2 cut(s) 512, 697
AspS9I GGNCC 2 cut(s) 65, 77
AsuC2I CCSGG 1 cut(s) 631
AsuHPI GGTGA 3 cut(s) 658, 733, 769
BalI TGGCCA 2 cut(s) 94, 497
BamHI GGATCC 1 cut(s) 55
BanI GGYRCC 2 cut(s) 509, 694
BarI GAAGNNNNNNTAC 2 cut(s) 24, 56
BauI CACGAG 1 cut(s) 107
BbvI GCAGC 2 cut(s) 443, 651
BccI CCATC 7 cut(s) 88, 190, 281, 430, 460, 470, 827
BciVI GTATCC 1 cut(s) 113
BcnI CCSGG 1 cut(s) 631
BfoI RGCGCY 2 cut(s) 513, 698
BfuI GTATCC 1 cut(s) 113
BisI GCNGC 2 cut(s) 432, 665
BlsI GCNGC 2 cut(s) 433, 666
Bme1390I CCNGG 1 cut(s) 631
BmgT120I GGNCC 2 cut(s) 65, 77
BmiI GGNNCC 3 cut(s) 57, 511, 696
BmrFI CCNGG 1 cut(s) 631
BmrI ACTGGG 1 cut(s) 917
BmuI ACTGGG 1 cut(s) 917
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BpmI CTGGAG 2 cut(s) 546, 594
BpuEI CTTGAG 1 cut(s) 271
BpuMI CCSGG 1 cut(s) 631
BsaBI GATNNNNATC 1 cut(s) 917
BsaHI GRCGYC 3 cut(s) 510, 695, 776
BsaJI CCNNGG 3 cut(s) 513, 630, 881
BsaXI ACNNNNNCTCC 5 cut(s) 26, 202, 232, 731, 761
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 227
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 3 cut(s) 563, 611, 923
Bse8I GATNNNNATC 1 cut(s) 917
BseDI CCNNGG 3 cut(s) 513, 630, 881
BseGI GGATG 2 cut(s) 273, 715
BseJI GATNNNNATC 1 cut(s) 917
BseLI CCNNNNNNNGG 2 cut(s) 74, 227
BseMII CTCAG 2 cut(s) 23, 531
BseNI ACTGG 3 cut(s) 563, 611, 923
BseRI GAGGAG 2 cut(s) 205, 704
BseXI GCAGC 2 cut(s) 443, 651
BseYI CCCAGC 2 cut(s) 36, 573
BshFI GGCC 4 cut(s) 66, 78, 94, 497
BshNI GGYRCC 2 cut(s) 509, 694
BsiSI CCGG 3 cut(s) 409, 630, 704
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 2 cut(s) 74, 227
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 4 cut(s) 66, 78, 94, 497
Bsp143I GATC 6 cut(s) 55, 230, 448, 594, 796, 918
BspACI CCGC 3 cut(s) 23, 686, 768
BspANI GGCC 4 cut(s) 66, 78, 94, 497
BspCNI CTCAG 2 cut(s) 22, 532
BspLI GGNNCC 3 cut(s) 57, 511, 696
BspPI GGATC 6 cut(s) 50, 63, 238, 443, 804, 913
BspT107I GGYRCC 2 cut(s) 509, 694
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 3 cut(s) 563, 611, 923
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 3 cut(s) 513, 630, 881
BssMI GATC 6 cut(s) 55, 230, 448, 594, 796, 918
BssNAI GTATAC 1 cut(s) 146
BssNI GRCGYC 3 cut(s) 510, 695, 776
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 2 cut(s) 513, 881
Bst1107I GTATAC 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 107
Bst4CI ACNGT 4 cut(s) 45, 101, 490, 646
Bst6I CTCTTC 2 cut(s) 172, 809
BstACI GRCGYC 3 cut(s) 510, 695, 776
BstAPI GCANNNNNTGC 1 cut(s) 661
BstC8I GCNNGC 4 cut(s) 495, 669, 864, 894
BstDEI CTNAG 3 cut(s) 9, 540, 672
BstF5I GGATG 2 cut(s) 273, 715
BstH2I RGCGCY 2 cut(s) 513, 698
BstHHI GCGC 2 cut(s) 512, 697
BstKTI GATC 6 cut(s) 58, 233, 451, 597, 799, 921
BstMBI GATC 6 cut(s) 55, 230, 448, 594, 796, 918
BstMWI GCNNNNNNNGC 5 cut(s) 557, 605, 635, 661, 692
BstSCI CCNGG 1 cut(s) 629
BstV1I GCAGC 2 cut(s) 443, 651
BstX2I RGATCY 1 cut(s) 55
BstXI CCANNNNNNTGG 1 cut(s) 87
BstYI RGATCY 1 cut(s) 55
BstZ17I GTATAC 1 cut(s) 146
BsuI GTATCC 1 cut(s) 113
BsuRI GGCC 4 cut(s) 66, 78, 94, 497
BtsCI GGATG 2 cut(s) 273, 715
BtsIMutI CAGTG 2 cut(s) 432, 462
Cac8I GCNNGC 4 cut(s) 495, 669, 864, 894
CfoI GCGC 2 cut(s) 512, 697
Cfr10I RCCGGY 1 cut(s) 703
Cfr13I GGNCC 2 cut(s) 65, 77
CseI GACGC 2 cut(s) 644, 765
Csp6I GTAC 2 cut(s) 41, 112
CviAII CATG 4 cut(s) 115, 331, 553, 601
CviQI GTAC 2 cut(s) 41, 112
DdeI CTNAG 3 cut(s) 9, 540, 672
DinI GGCGCC 2 cut(s) 511, 696
DpnI GATC 6 cut(s) 57, 232, 450, 596, 798, 920
DpnII GATC 6 cut(s) 55, 230, 448, 594, 796, 918
EaeI YGGCCR 2 cut(s) 92, 495
Eam1104I CTCTTC 2 cut(s) 172, 809
EarI CTCTTC 2 cut(s) 172, 809
EciI GGCGGA 1 cut(s) 701
Eco130I CCWWGG 2 cut(s) 513, 881
Eco57I CTGAAG 1 cut(s) 833
EcoT14I CCWWGG 2 cut(s) 513, 881
EgeI GGCGCC 2 cut(s) 511, 696
EheI GGCGCC 2 cut(s) 511, 696
ErhI CCWWGG 2 cut(s) 513, 881
FaeI CATG 4 cut(s) 118, 334, 556, 604
FaqI GGGAC 1 cut(s) 647
FatI CATG 4 cut(s) 114, 330, 552, 600
FblI GTMKAC 1 cut(s) 145
Fnu4HI GCNGC 2 cut(s) 432, 665
FokI GGATG 2 cut(s) 260, 722
Fsp4HI GCNGC 2 cut(s) 432, 665
GlaI GCGC 2 cut(s) 511, 696
GluI GCNGC 2 cut(s) 432, 665
GsaI CCCAGC 2 cut(s) 40, 577
GsuI CTGGAG 2 cut(s) 546, 594
HaeII RGCGCY 2 cut(s) 513, 698
HaeIII GGCC 4 cut(s) 66, 78, 94, 497
HapII CCGG 3 cut(s) 409, 630, 704
HgaI GACGC 2 cut(s) 644, 765
HhaI GCGC 2 cut(s) 512, 697
Hin1I GRCGYC 3 cut(s) 510, 695, 776
Hin1II CATG 4 cut(s) 118, 334, 556, 604
Hin6I GCGC 2 cut(s) 510, 695
HinP1I GCGC 2 cut(s) 510, 695
HinfI GANTC 2 cut(s) 7, 520
HpaII CCGG 3 cut(s) 409, 630, 704
HphI GGTGA 3 cut(s) 658, 733, 769
Hpy166II GTNNAC 2 cut(s) 146, 486
Hpy188I TCNGA 3 cut(s) 55, 681, 781
Hpy188III TCNNGA 2 cut(s) 59, 139
Hpy8I GTNNAC 2 cut(s) 146, 486
HpyAV CCTTC 3 cut(s) 26, 56, 676
HpyCH4III ACNGT 4 cut(s) 45, 101, 490, 646
HpyCH4V TGCA 3 cut(s) 419, 655, 896
HpyF10VI GCNNNNNNNGC 5 cut(s) 557, 605, 635, 661, 692
HpyF3I CTNAG 3 cut(s) 9, 540, 672
Hsp92I GRCGYC 3 cut(s) 510, 695, 776
Hsp92II CATG 4 cut(s) 118, 334, 556, 604
HspAI GCGC 2 cut(s) 510, 695
KasI GGCGCC 2 cut(s) 509, 694
Kzo9I GATC 6 cut(s) 55, 230, 448, 594, 796, 918
LmnI GCTCC 2 cut(s) 565, 613
Lsp1109I GCAGC 2 cut(s) 443, 651
MaeIII GTNAC 3 cut(s) 616, 646, 757
MalI GATC 6 cut(s) 57, 232, 450, 596, 798, 920
MboI GATC 6 cut(s) 55, 230, 448, 594, 796, 918
MboII GAAGA 3 cut(s) 159, 556, 826
MflI RGATCY 1 cut(s) 55
MlsI TGGCCA 2 cut(s) 94, 497
MluCI AATT 6 cut(s) 156, 268, 322, 351, 499, 656
MluNI TGGCCA 2 cut(s) 94, 497
Mly113I GGCGCC 2 cut(s) 510, 695
MmeI TCCRAC 2 cut(s) 185, 351
Mox20I TGGCCA 2 cut(s) 94, 497
MscI TGGCCA 2 cut(s) 94, 497
MseI TTAA 2 cut(s) 384, 840
MslI CAYNNNNRTG 2 cut(s) 85, 335
Msp20I TGGCCA 2 cut(s) 94, 497
MspI CCGG 3 cut(s) 409, 630, 704
MspR9I CCNGG 1 cut(s) 631
MwoI GCNNNNNNNGC 5 cut(s) 557, 605, 635, 661, 692
NarI GGCGCC 2 cut(s) 510, 695
NciI CCSGG 1 cut(s) 631
NdeII GATC 6 cut(s) 55, 230, 448, 594, 796, 918
NlaIII CATG 4 cut(s) 118, 334, 556, 604
NlaIV GGNNCC 3 cut(s) 57, 511, 696
NmeAIII GCCGAG 1 cut(s) 563
NmuCI GTSAC 3 cut(s) 616, 646, 757
PfeI GAWTC 1 cut(s) 520
PkrI GCNGC 2 cut(s) 433, 666
PluTI GGCGCC 2 cut(s) 513, 698
PspFI CCCAGC 2 cut(s) 36, 573
PspN4I GGNNCC 3 cut(s) 57, 511, 696
PspPI GGNCC 2 cut(s) 65, 77
PsuI RGATCY 1 cut(s) 55
RsaI GTAC 2 cut(s) 42, 113
RsaNI GTAC 2 cut(s) 41, 112
RseI CAYNNNNRTG 2 cut(s) 85, 335
SaqAI TTAA 2 cut(s) 384, 840
SatI GCNGC 2 cut(s) 432, 665
Sau3AI GATC 6 cut(s) 55, 230, 448, 594, 796, 918
Sau96I GGNCC 2 cut(s) 65, 77
ScrFI CCNGG 1 cut(s) 631
SfoI GGCGCC 2 cut(s) 511, 696
SmiMI CAYNNNNRTG 2 cut(s) 85, 335
SmlI CTYRAG 1 cut(s) 286
SmoI CTYRAG 1 cut(s) 286
Sse9I AATT 6 cut(s) 156, 268, 322, 351, 499, 656
SsiI CCGC 3 cut(s) 23, 686, 768
SspDI GGCGCC 2 cut(s) 509, 694
StyD4I CCNGG 1 cut(s) 629
StyI CCWWGG 2 cut(s) 513, 881
TaaI ACNGT 4 cut(s) 45, 101, 490, 646
TaqI TCGA 1 cut(s) 83
TaqII GACCGA 1 cut(s) 134
TasI AATT 6 cut(s) 156, 268, 322, 351, 499, 656
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 1 cut(s) 520
Tru1I TTAA 2 cut(s) 384, 840
Tru9I TTAA 2 cut(s) 384, 840
TscAI CASTG 2 cut(s) 432, 462
TseFI GTSAC 3 cut(s) 616, 646, 757
TseI GCWGC 2 cut(s) 431, 664
Tsp45I GTSAC 3 cut(s) 616, 646, 757
TspRI CASTG 2 cut(s) 432, 462
XapI RAATTY 4 cut(s) 156, 268, 322, 351
XcmI CCANNNNNNNNNTGG 2 cut(s) 87, 334
XmiI GTMKAC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.