FvH4_6g16520

Thioredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013436
Physical Location & Seq
Reverse (-)
10450521 .. 10453273
2753 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16520.t2

Sequence Viewer

Length: 396 bp
ATGGAGGCCGGAACTGGGTGGACCAGCGACGACGCCAACACCAAGAACGGCTTAAGTTTGCCGGCGAATCGGCATGGGAACCTAAAGACTGCCTCTAGCGATCAAAACCTCAGTAACATTCTTCTTCAAATCAAGTCCTCCAAAACCCCTGCAGTCATCAATTACGGCGCAACCTGGTGCGGAGTGTGTAGCCAGATTCTTCCCGCGTTCTGCAAGCTGAGTGATAGTTTCCCAAAGTTTTCGTTTGTCTATGCAGATATTGATGAGTGCCCTGAAACTACTCAGCACATTCGGTACACGCCGACCTTCCATTTCTACAGAGATGGTGAGAGGGTTGATGAGATGTTTGGTGCCGGCGAAGAGCGGCTTCATGATCGCTTGTGGTTGCATTCTTGA

Protein Analysis

132

Amino Acids

14.69

Weight (kDa)

5.65

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 43 - 124 2.5e-15 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53220
fragaria_vesca FvH4_6g16520 FvH4_6g16520
malus_domestica MD04G1091800.v1.1 MD12G1109800.v1.1
prunus_persica Prupe.7G128400_v2.0.a1
pyrus_communis pycom12g11070
rosa_chinensis RchiOBHm_Chr3g0470431
rosa_laevigata RLG00000024268
rosa_multiflora Rmu_sc0002383.1_g000008 Rmu_sc0036044.1_g000001
rosa_roxburghii Rroxscaffold_6G00410820
rosa_rugosa Rorug03G0108800
rosa_samantha Rh3AG159200 Rh3BG183700 Rh3CG174200 Rh3DG191100
rosa_wichuraiana Rw3G015080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 350
AccBSI CCGCTC 1 cut(s) 364
AccII CGCG 1 cut(s) 206
AciI CCGC 3 cut(s) 180, 204, 364
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 15
AflII CTTAAG 1 cut(s) 52
AgsI TTSAA 1 cut(s) 128
AjnI CCWGG 1 cut(s) 173
AluBI AGCT 1 cut(s) 217
AluI AGCT 1 cut(s) 217
AoxI GGCC 1 cut(s) 6
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 1 cut(s) 21
AsuHPI GGTGA 1 cut(s) 338
AvaII GGWCC 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 350
BccI CCATC 1 cut(s) 317
BceAI ACGGC 2 cut(s) 64, 181
BciT130I CCWGG 1 cut(s) 175
BfaI CTAG 1 cut(s) 96
BfmI CTRYAG 2 cut(s) 150, 316
BfrI CTTAAG 1 cut(s) 52
BisI GCNGC 1 cut(s) 365
BlsI GCNGC 1 cut(s) 366
Bme1390I CCNGG 1 cut(s) 175
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 21
BmiI GGNNCC 2 cut(s) 80, 352
BmrFI CCNGG 1 cut(s) 175
BmrI ACTGGG 1 cut(s) 24
BmuI ACTGGG 1 cut(s) 24
BsaHI GRCGYC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse118I RCCGGY 2 cut(s) 61, 353
Bse1I ACTGG 1 cut(s) 19
BseBI CCWGG 1 cut(s) 175
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMII CTCAG 3 cut(s) 124, 209, 296
BseNI ACTGG 1 cut(s) 19
BseSI GKGCMC 1 cut(s) 272
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 1 cut(s) 8
BshNI GGYRCC 1 cut(s) 350
BsiSI CCGG 3 cut(s) 9, 62, 354
BslI CCNNNNNNNGG 1 cut(s) 15
BsmI GAATGC 1 cut(s) 388
BsnI GGCC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 272
Bsp143I GATC 2 cut(s) 100, 373
BspACI CCGC 3 cut(s) 180, 204, 364
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 3 cut(s) 123, 210, 295
BspFNI CGCG 1 cut(s) 206
BspHI TCATGA 1 cut(s) 370
BspLI GGNNCC 2 cut(s) 80, 352
BspMAI CTGCAG 1 cut(s) 154
BspQI GCTCTTC 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 350
BspTI CTTAAG 1 cut(s) 52
BsrBI CCGCTC 1 cut(s) 364
BsrFI RCCGGY 2 cut(s) 61, 353
BsrI ACTGG 1 cut(s) 19
BssAI RCCGGY 2 cut(s) 61, 353
BssMI GATC 2 cut(s) 100, 373
BssNI GRCGYC 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 175
Bst6I CTCTTC 1 cut(s) 354
BstACI GRCGYC 1 cut(s) 33
BstAFI CTTAAG 1 cut(s) 52
BstC8I GCNNGC 3 cut(s) 63, 215, 355
BstDEI CTNAG 3 cut(s) 110, 218, 282
BstFNI CGCG 1 cut(s) 206
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 2 cut(s) 103, 376
BstMBI GATC 2 cut(s) 100, 373
BstNI CCWGG 1 cut(s) 175
BstSCI CCNGG 1 cut(s) 173
BstSFI CTRYAG 2 cut(s) 150, 316
BstSLI GKGCMC 1 cut(s) 272
BstUI CGCG 1 cut(s) 206
BsuRI GGCC 1 cut(s) 8
Cac8I GCNNGC 3 cut(s) 63, 215, 355
CciI TCATGA 1 cut(s) 370
CfoI GCGC 1 cut(s) 170
Cfr10I RCCGGY 2 cut(s) 61, 353
Cfr13I GGNCC 1 cut(s) 21
CseI GACGC 1 cut(s) 41
CsiI ACCWGGT 1 cut(s) 173
Csp6I GTAC 1 cut(s) 295
CviAII CATG 2 cut(s) 74, 371
CviJI RGCY 5 cut(s) 8, 51, 192, 217, 367
CviKI_1 RGCY 5 cut(s) 8, 51, 192, 217, 367
CviQI GTAC 1 cut(s) 295
DdeI CTNAG 3 cut(s) 110, 218, 282
DpnI GATC 2 cut(s) 102, 375
DpnII GATC 2 cut(s) 100, 373
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 173
FaeI CATG 2 cut(s) 77, 374
FaiI YATR 3 cut(s) 75, 252, 372
FalI AAGNNNNNCTT 4 cut(s) 35, 67, 351, 383
FatI CATG 2 cut(s) 73, 370
FauI CCCGC 1 cut(s) 211
Fnu4HI GCNGC 1 cut(s) 365
Fsp4HI GCNGC 1 cut(s) 365
FspBI CTAG 1 cut(s) 96
GlaI GCGC 1 cut(s) 169
GluI GCNGC 1 cut(s) 365
HaeIII GGCC 1 cut(s) 8
HapII CCGG 3 cut(s) 9, 62, 354
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 170
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 2 cut(s) 77, 374
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HinfI GANTC 2 cut(s) 67, 196
HpaII CCGG 3 cut(s) 9, 62, 354
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 2 cut(s) 21, 297
Hpy188III TCNNGA 2 cut(s) 371, 393
Hpy8I GTNNAC 2 cut(s) 21, 297
Hpy99I CGWCG 2 cut(s) 32, 35
HpyAV CCTTC 1 cut(s) 316
HpyCH4V TGCA 4 cut(s) 152, 213, 254, 388
HpyF3I CTNAG 3 cut(s) 110, 218, 282
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 2 cut(s) 77, 374
HspAI GCGC 1 cut(s) 168
KroI GCCGGC 2 cut(s) 61, 353
KroNI GCCGGC 2 cut(s) 63, 355
Kzo9I GATC 2 cut(s) 100, 373
LguI GCTCTTC 1 cut(s) 354
LpnPI CCDG 9 cut(s) 22, 37, 75, 160, 162, 187, 206, 285, 367
MabI ACCWGGT 1 cut(s) 173
MaeI CTAG 1 cut(s) 96
MaeIII GTNAC 1 cut(s) 113
MalI GATC 2 cut(s) 102, 375
MbiI CCGCTC 1 cut(s) 364
MboI GATC 2 cut(s) 100, 373
MboII GAAGA 4 cut(s) 113, 116, 191, 371
MhlI GDGCHC 1 cut(s) 272
MluCI AATT 1 cut(s) 160
MnlI CCTC 4 cut(s) 103, 119, 148, 324
MroNI GCCGGC 2 cut(s) 61, 353
MseI TTAA 1 cut(s) 53
MspCI CTTAAG 1 cut(s) 52
MspI CCGG 3 cut(s) 9, 62, 354
MspR9I CCNGG 1 cut(s) 175
Mva1269I GAATGC 1 cut(s) 388
MvaI CCWGG 1 cut(s) 175
MvnI CGCG 1 cut(s) 206
NaeI GCCGGC 2 cut(s) 63, 355
NdeII GATC 2 cut(s) 100, 373
NgoMIV GCCGGC 2 cut(s) 61, 353
NlaIII CATG 2 cut(s) 77, 374
NlaIV GGNNCC 2 cut(s) 80, 352
PagI TCATGA 1 cut(s) 370
PciSI GCTCTTC 1 cut(s) 354
PctI GAATGC 1 cut(s) 388
PdiI GCCGGC 2 cut(s) 63, 355
PfeI GAWTC 2 cut(s) 67, 196
PkrI GCNGC 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 173
PspGI CCWGG 1 cut(s) 173
PspN4I GGNNCC 2 cut(s) 80, 352
PspPI GGNCC 1 cut(s) 21
PstI CTGCAG 1 cut(s) 154
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 1 cut(s) 53
SatI GCNGC 1 cut(s) 365
Sau3AI GATC 2 cut(s) 100, 373
Sau96I GGNCC 1 cut(s) 21
ScrFI CCNGG 1 cut(s) 175
SduI GDGCHC 1 cut(s) 272
SetI ASST 5 cut(s) 84, 111, 176, 219, 308
SexAI ACCWGGT 1 cut(s) 173
SfcI CTRYAG 2 cut(s) 150, 316
SinI GGWCC 1 cut(s) 21
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 1 cut(s) 160
SsiI CCGC 3 cut(s) 180, 204, 364
SspMI CTAG 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 173
TasI AATT 1 cut(s) 160
TauI GCSGC 1 cut(s) 367
TfiI GAWTC 2 cut(s) 67, 196
Tru1I TTAA 1 cut(s) 53
Tru9I TTAA 1 cut(s) 53
TspDTI ATGAA 1 cut(s) 359
Vha464I CTTAAG 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 21
XspI CTAG 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.