RLG00000024268

Thioredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013436
Physical Location & Seq
Forward (+)
33724300 .. 33724964
665 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024268

Sequence Viewer

Length: 417 bp
ATGGAGGGAGGAACTGGGTCGACCGGCGACGACGCCGACACCAACACCAACAACGGCTTAGACGGCACAACCTTCAATTTTCCGGCGAATCGCCATGGGAACCTAAAGACTGCGTCTAGTGATCAAAGCCTCAGCGACATTCTTCTTCAAATCAAGTCCTCCAAAACCCCTGCAGTTATCAATTACGGCGCAACCTGGTGTCGAGTGTGCAGCCAGATTCTTCCTGCATTCTGCAAGCTGAGTAATAGTTTCCCAAAGCTCTCGTTCGTCTATGCAGATATTGATGAATGCCCTGAAACAACTCAGCACATTCGGTATACGCCGACGTTCCATTTCTACAGAGATGGTGAGAGGGTGGATGAGATGTTTGGTGCCGGCGAAGAGCGGCTTCATGATCGCTTGTGGTTGCACTCTTGA

Protein Analysis

139

Amino Acids

15.37

Weight (kDa)

5.22

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 50 - 131 7.4e-14 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53220
fragaria_vesca FvH4_6g16520 FvH4_6g16520
malus_domestica MD04G1091800.v1.1 MD12G1109800.v1.1
prunus_persica Prupe.7G128400_v2.0.a1
pyrus_communis pycom12g11070
rosa_chinensis RchiOBHm_Chr3g0470431
rosa_laevigata RLG00000024268
rosa_multiflora Rmu_sc0002383.1_g000008 Rmu_sc0036044.1_g000001
rosa_roxburghii Rroxscaffold_6G00410820
rosa_rugosa Rorug03G0108800
rosa_samantha Rh3AG159200 Rh3BG183700 Rh3CG174200 Rh3DG191100
rosa_wichuraiana Rw3G015080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 371
AccBSI CCGCTC 1 cut(s) 385
AccI GTMKAC 2 cut(s) 20, 317
AciI CCGC 1 cut(s) 385
AcyI GRCGYC 1 cut(s) 33
AgsI TTSAA 2 cut(s) 76, 149
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 2 cut(s) 238, 259
AluI AGCT 2 cut(s) 238, 259
ApeKI GCWGC 1 cut(s) 210
AspLEI GCGC 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 359
BanI GGYRCC 1 cut(s) 371
BbvCI CCTCAGC 1 cut(s) 131
BbvI GCAGC 1 cut(s) 222
BccI CCATC 1 cut(s) 338
BceAI ACGGC 3 cut(s) 70, 79, 202
BciT130I CCWGG 1 cut(s) 196
BclI TGATCA 1 cut(s) 121
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 2 cut(s) 171, 337
BisI GCNGC 2 cut(s) 211, 386
BlsI GCNGC 2 cut(s) 212, 387
Bme1390I CCNGG 1 cut(s) 196
BmiI GGNNCC 2 cut(s) 101, 373
BmrFI CCNGG 1 cut(s) 196
BmrI ACTGGG 1 cut(s) 24
BmuI ACTGGG 1 cut(s) 24
Bpu10I CCTNAGC 1 cut(s) 131
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 94
Bse118I RCCGGY 2 cut(s) 23, 374
Bse1I ACTGG 1 cut(s) 19
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 94
BseGI GGATG 1 cut(s) 364
BseMII CTCAG 3 cut(s) 145, 230, 317
BseNI ACTGG 1 cut(s) 19
BseXI GCAGC 1 cut(s) 222
BsgI GTGCAG 1 cut(s) 229
Bsh1285I CGRYCG 1 cut(s) 24
BshNI GGYRCC 1 cut(s) 371
BsiEI CGRYCG 1 cut(s) 24
BsiSI CCGG 3 cut(s) 24, 83, 375
BsmI GAATGC 2 cut(s) 227, 293
Bsp143I GATC 2 cut(s) 121, 394
Bsp19I CCATGG 1 cut(s) 94
BspACI CCGC 1 cut(s) 385
BspCNI CTCAG 3 cut(s) 144, 231, 316
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 2 cut(s) 101, 373
BspMAI CTGCAG 1 cut(s) 175
BspQI GCTCTTC 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 371
BsrBI CCGCTC 1 cut(s) 385
BsrFI RCCGGY 2 cut(s) 23, 374
BsrI ACTGG 1 cut(s) 19
BssAI RCCGGY 2 cut(s) 23, 374
BssECI CCNNGG 1 cut(s) 94
BssMI GATC 2 cut(s) 121, 394
BssNAI GTATAC 1 cut(s) 318
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 94
Bst1107I GTATAC 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 196
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 236, 376
BstDEI CTNAG 4 cut(s) 58, 131, 239, 303
BstDSI CCRYGG 1 cut(s) 94
BstF5I GGATG 1 cut(s) 364
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 2 cut(s) 124, 397
BstMBI GATC 2 cut(s) 121, 394
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 1 cut(s) 194
BstSFI CTRYAG 2 cut(s) 171, 337
BstV1I GCAGC 1 cut(s) 222
BstZ17I GTATAC 1 cut(s) 318
BtgI CCRYGG 1 cut(s) 94
BtsCI GGATG 1 cut(s) 364
Cac8I GCNNGC 2 cut(s) 236, 376
CciI TCATGA 1 cut(s) 391
CfoI GCGC 1 cut(s) 191
Cfr10I RCCGGY 2 cut(s) 23, 374
CseI GACGC 2 cut(s) 41, 102
CsiI ACCWGGT 1 cut(s) 194
CviAII CATG 2 cut(s) 95, 392
CviJI RGCY 6 cut(s) 57, 129, 213, 238, 259, 388
CviKI_1 RGCY 6 cut(s) 57, 129, 213, 238, 259, 388
DdeI CTNAG 4 cut(s) 58, 131, 239, 303
DpnI GATC 2 cut(s) 123, 396
DpnII GATC 2 cut(s) 121, 394
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 94
FaeI CATG 2 cut(s) 98, 395
FaiI YATR 4 cut(s) 96, 273, 318, 393
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FatI CATG 2 cut(s) 94, 391
FbaI TGATCA 1 cut(s) 121
FblI GTMKAC 2 cut(s) 20, 317
Fnu4HI GCNGC 2 cut(s) 211, 386
FokI GGATG 1 cut(s) 371
Fsp4HI GCNGC 2 cut(s) 211, 386
FspBI CTAG 1 cut(s) 117
GlaI GCGC 1 cut(s) 190
GluI GCNGC 2 cut(s) 211, 386
HapII CCGG 3 cut(s) 24, 83, 375
HgaI GACGC 2 cut(s) 41, 102
HhaI GCGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 2 cut(s) 98, 395
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 2 cut(s) 88, 217
HpaII CCGG 3 cut(s) 24, 83, 375
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 2 cut(s) 21, 318
Hpy188III TCNNGA 2 cut(s) 392, 414
Hpy8I GTNNAC 2 cut(s) 21, 318
Hpy99I CGWCG 3 cut(s) 32, 35, 328
HpyAV CCTTC 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 326
HpyCH4V TGCA 6 cut(s) 173, 210, 227, 234, 275, 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 4 cut(s) 58, 131, 239, 303
HpySE526I ACGT 1 cut(s) 326
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 2 cut(s) 98, 395
HspAI GCGC 1 cut(s) 189
KroI GCCGGC 1 cut(s) 374
KroNI GCCGGC 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 2 cut(s) 121, 394
LguI GCTCTTC 1 cut(s) 375
LpnPI CCDG 9 cut(s) 37, 96, 181, 183, 208, 227, 237, 306, 388
Lsp1109I GCAGC 1 cut(s) 222
MabI ACCWGGT 1 cut(s) 194
MaeI CTAG 1 cut(s) 117
MaeII ACGT 1 cut(s) 326
MalI GATC 2 cut(s) 123, 396
MbiI CCGCTC 1 cut(s) 385
MboI GATC 2 cut(s) 121, 394
MboII GAAGA 4 cut(s) 134, 137, 212, 392
MluCI AATT 2 cut(s) 76, 181
MnlI CCTC 3 cut(s) 140, 169, 345
MroNI GCCGGC 1 cut(s) 374
MspI CCGG 3 cut(s) 24, 83, 375
MspR9I CCNGG 1 cut(s) 196
Mva1269I GAATGC 2 cut(s) 227, 293
MvaI CCWGG 1 cut(s) 196
MwoI GCNNNNNNNGC 1 cut(s) 63
NaeI GCCGGC 1 cut(s) 376
NcoI CCATGG 1 cut(s) 94
NdeII GATC 2 cut(s) 121, 394
NgoMIV GCCGGC 1 cut(s) 374
NlaIII CATG 2 cut(s) 98, 395
NlaIV GGNNCC 2 cut(s) 101, 373
PagI TCATGA 1 cut(s) 391
PciSI GCTCTTC 1 cut(s) 375
PctI GAATGC 2 cut(s) 227, 293
PdiI GCCGGC 1 cut(s) 376
PfeI GAWTC 2 cut(s) 88, 217
PflFI GACNNNGTC 1 cut(s) 112
PkrI GCNGC 2 cut(s) 212, 387
Psp6I CCWGG 1 cut(s) 194
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 2 cut(s) 101, 373
PstI CTGCAG 1 cut(s) 175
PsyI GACNNNGTC 1 cut(s) 112
SalI GTCGAC 1 cut(s) 19
SapI GCTCTTC 1 cut(s) 375
SatI GCNGC 2 cut(s) 211, 386
Sau3AI GATC 2 cut(s) 121, 394
ScrFI CCNGG 1 cut(s) 196
SetI ASST 6 cut(s) 74, 105, 197, 240, 261, 329
SexAI ACCWGGT 1 cut(s) 194
SfcI CTRYAG 2 cut(s) 171, 337
Sse9I AATT 2 cut(s) 76, 181
SsiI CCGC 1 cut(s) 385
SspMI CTAG 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 194
StyI CCWWGG 1 cut(s) 94
TaiI ACGT 1 cut(s) 329
TaqI TCGA 2 cut(s) 20, 202
TasI AATT 2 cut(s) 76, 181
TauI GCSGC 1 cut(s) 388
TfiI GAWTC 2 cut(s) 88, 217
TseI GCWGC 1 cut(s) 210
TspDTI ATGAA 2 cut(s) 300, 380
Tth111I GACNNNGTC 1 cut(s) 112
XmiI GTMKAC 2 cut(s) 20, 317
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.