FvH4_6g20170

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017103
Physical Location & Seq
Reverse (-)
13712263 .. 13712895
633 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20170.t1

Sequence Viewer

Length: 633 bp
ATGTCTTCGAAAGACTGCGGCCAGCACGGCCACAACAAGCGGCGAAAGAGAATCCGCCACTGCTGCGCGGGGCTCCTGATCTTCAACTTCATCCTCCTCATCACCATCCTCATCATCTGGGCGATCCTCCAGCCGTCCAAGCCCCGCTTCATCCTCCAGGACGTCACCGTCTTCAACTTCAACAACACCGTCCCCAACGTCTTCTCCTCCAGCTTCCAGGTCACCATCTCCTCCCGCAACCCCAACGACAAGCTCGGTATCTACTACGACAGGCTCGACGTCTACGCCGTCTACCGCTCCCAGCAGATCACCTTCTCAACCCTAATCCCGCCAACCTACCAAGGCCACAAGGACGTCAACATCTGGTCGCCCTTCGTCTACGGCACCAACGTCCCCATCGCTCCCTACAACTCCCTCGCTCTCGCACAGGACCAGGCCTCCGGCAGCGTCCTTTTGTTGATCAAGATCGACGGCCGGGTGAGATGGAAGATTGGAACATTCATCACCGGAAAGTACCATCTCTATGTGAGATGTCCGGCTTTCATAACTTTGGGAGCCAAGACTACCGGAATTGTGGTCGGAGAAAACGCTGTCAAGTACCAGCTGGTCCAGCGATGCAAGGTAAGCATATGA

Protein Analysis

211

Amino Acids

23.76

Weight (kDa)

9.88

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 75 - 177 1e-09 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11660
fragaria_vesca FvH4_6g20170
malus_domestica MD12G1038700.v1.1 MD14G1037400.v1.1
prunus_persica Prupe.7G097100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475681
rosa_laevigata RLG00000023824
rosa_multiflora Rmu_sc0000539.1_g000056
rosa_roxburghii Rroxscaffold_6G00406000
rosa_rugosa Rorug03G0150800
rosa_samantha Rh3CG225400 Rh3DG225900
rosa_wichuraiana Rw3G018210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AatII GACGTC 3 cut(s) 165, 282, 357
AccB1I GGYRCC 1 cut(s) 383
AccBSI CCGCTC 1 cut(s) 297
AccI GTMKAC 3 cut(s) 282, 291, 378
AccII CGCG 1 cut(s) 68
AciI CCGC 8 cut(s) 18, 40, 55, 68, 145, 235, 295, 329
AclWI GGATC 1 cut(s) 118
AcoI YGGCCR 3 cut(s) 19, 28, 472
AcyI GRCGYC 3 cut(s) 162, 279, 354
AfaI GTAC 2 cut(s) 515, 599
AgsI TTSAA 3 cut(s) 85, 175, 181
AjnI CCWGG 3 cut(s) 156, 216, 432
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AluBI AGCT 3 cut(s) 213, 253, 604
AluI AGCT 3 cut(s) 213, 253, 604
AlwI GGATC 1 cut(s) 118
AoxI GGCC 5 cut(s) 19, 28, 343, 435, 472
ApeKI GCWGC 2 cut(s) 63, 444
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 2 cut(s) 430, 607
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 6 cut(s) 94, 157, 214, 301, 490, 496
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 2 cut(s) 430, 607
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 75
BbsI GAAGAC 2 cut(s) 163, 193
BbvI GCAGC 2 cut(s) 50, 456
BccI CCATC 5 cut(s) 113, 233, 404, 477, 525
BceAI ACGGC 5 cut(s) 43, 118, 272, 397, 487
BciT130I CCWGG 3 cut(s) 158, 218, 434
BclI TGATCA 1 cut(s) 459
BcnI CCSGG 1 cut(s) 476
BglI GCCNNNNNGGC 1 cut(s) 27
BisI GCNGC 4 cut(s) 19, 41, 64, 445
BlsI GCNGC 4 cut(s) 20, 42, 65, 446
Bme1390I CCNGG 4 cut(s) 158, 218, 434, 476
Bme18I GGWCC 2 cut(s) 430, 607
BmgT120I GGNCC 2 cut(s) 430, 607
BmiI GGNNCC 3 cut(s) 74, 385, 556
BmrFI CCNGG 4 cut(s) 158, 218, 434, 476
BmsI GCATC 1 cut(s) 605
BpiI GAAGAC 2 cut(s) 163, 193
BpmI CTGGAG 3 cut(s) 113, 140, 193
Bpu14I TTCGAA 1 cut(s) 8
BpuMI CCSGG 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 464
BsaHI GRCGYC 3 cut(s) 162, 279, 354
BsaJI CCNNGG 1 cut(s) 340
BsaWI WCCGGW 2 cut(s) 506, 566
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 422, 452
Bse8I GATNNNNATC 1 cut(s) 464
BseBI CCWGG 3 cut(s) 158, 218, 434
BseDI CCNNGG 1 cut(s) 340
BseGI GGATG 3 cut(s) 90, 105, 150
BseJI GATNNNNATC 1 cut(s) 464
BseRI GAGGAG 3 cut(s) 86, 196, 220
BseX3I CGGCCG 1 cut(s) 472
BseXI GCAGC 2 cut(s) 50, 456
BseYI CCCAGC 1 cut(s) 300
Bsh1236I CGCG 1 cut(s) 68
Bsh1285I CGRYCG 1 cut(s) 475
BshFI GGCC 5 cut(s) 21, 30, 345, 437, 474
BshNI GGYRCC 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 475
BsiSI CCGG 5 cut(s) 441, 475, 507, 536, 567
BslFI GGGAC 2 cut(s) 176, 377
BsmFI GGGAC 2 cut(s) 176, 377
BsnI GGCC 5 cut(s) 21, 30, 345, 437, 474
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 5 cut(s) 78, 123, 306, 459, 465
BspACI CCGC 8 cut(s) 18, 40, 55, 68, 145, 235, 295, 329
BspANI GGCC 5 cut(s) 21, 30, 345, 437, 474
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 3 cut(s) 74, 385, 556
BspPI GGATC 1 cut(s) 118
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 383
BsrBI CCGCTC 1 cut(s) 297
BssECI CCNNGG 1 cut(s) 340
BssMI GATC 5 cut(s) 78, 123, 306, 459, 465
BssNI GRCGYC 3 cut(s) 162, 279, 354
BssT1I CCWWGG 1 cut(s) 340
Bst2UI CCWGG 3 cut(s) 158, 218, 434
Bst4CI ACNGT 2 cut(s) 169, 190
BstACI GRCGYC 3 cut(s) 162, 279, 354
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 23
BstEII GGTNACC 1 cut(s) 220
BstF5I GGATG 3 cut(s) 90, 105, 150
BstFNI CGCG 1 cut(s) 68
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 5 cut(s) 81, 126, 309, 462, 468
BstMBI GATC 5 cut(s) 78, 123, 306, 459, 465
BstMCI CGRYCG 1 cut(s) 475
BstMWI GCNNNNNNNGC 5 cut(s) 27, 63, 139, 610, 624
BstNI CCWGG 3 cut(s) 158, 218, 434
BstPI GGTNACC 1 cut(s) 220
BstSCI CCNGG 4 cut(s) 156, 216, 432, 474
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 50, 456
BstV2I GAAGAC 2 cut(s) 163, 193
BstZI CGGCCG 1 cut(s) 472
BsuRI GGCC 5 cut(s) 21, 30, 345, 437, 474
BtgZI GCGATG 2 cut(s) 382, 628
BtsCI GGATG 3 cut(s) 90, 105, 150
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 58
Cac8I GCNNGC 1 cut(s) 23
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 2 cut(s) 430, 607
CseI GACGC 1 cut(s) 436
Csp6I GTAC 2 cut(s) 514, 598
CviQI GTAC 2 cut(s) 514, 598
DpnI GATC 5 cut(s) 80, 125, 308, 461, 467
DpnII GATC 5 cut(s) 78, 123, 306, 459, 465
DrdI GACNNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 3 cut(s) 19, 28, 472
EagI CGGCCG 1 cut(s) 472
EciI GGCGGA 1 cut(s) 44
EclXI CGGCCG 1 cut(s) 472
Eco130I CCWWGG 1 cut(s) 340
Eco147I AGGCCT 1 cut(s) 437
Eco24I GRGCYC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 430, 607
Eco52I CGGCCG 1 cut(s) 472
Eco91I GGTNACC 1 cut(s) 220
EcoO65I GGTNACC 1 cut(s) 220
EcoRII CCWGG 3 cut(s) 156, 216, 432
EcoT14I CCWWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 340
FaiI YATR 4 cut(s) 525, 545, 629, 631
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FaqI GGGAC 2 cut(s) 176, 377
FauI CCCGC 4 cut(s) 61, 152, 242, 336
FauNDI CATATG 1 cut(s) 629
FbaI TGATCA 1 cut(s) 459
FblI GTMKAC 3 cut(s) 282, 291, 378
Fnu4HI GCNGC 4 cut(s) 19, 41, 64, 445
FokI GGATG 3 cut(s) 77, 92, 137
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 4 cut(s) 19, 41, 64, 445
GlaI GCGC 1 cut(s) 67
GluI GCNGC 4 cut(s) 19, 41, 64, 445
GsaI CCCAGC 1 cut(s) 304
GsuI CTGGAG 3 cut(s) 113, 140, 193
HaeIII GGCC 5 cut(s) 21, 30, 345, 437, 474
HapII CCGG 5 cut(s) 441, 475, 507, 536, 567
HgaI GACGC 1 cut(s) 436
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 3 cut(s) 162, 279, 354
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 1 cut(s) 51
HpaII CCGG 5 cut(s) 441, 475, 507, 536, 567
HphI GGTGA 6 cut(s) 94, 157, 214, 301, 490, 496
Hpy166II GTNNAC 4 cut(s) 283, 292, 358, 379
Hpy188I TCNGA 1 cut(s) 581
Hpy188III TCNNGA 2 cut(s) 76, 463
Hpy8I GTNNAC 4 cut(s) 283, 292, 358, 379
Hpy99I CGWCG 2 cut(s) 281, 473
HpyAV CCTTC 2 cut(s) 322, 382
HpyCH4III ACNGT 2 cut(s) 169, 190
HpyCH4IV ACGT 5 cut(s) 162, 198, 279, 354, 390
HpyCH4V TGCA 1 cut(s) 618
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 63, 139, 610, 624
HpySE526I ACGT 5 cut(s) 162, 198, 279, 354, 390
Hsp92I GRCGYC 3 cut(s) 162, 279, 354
HspAI GCGC 1 cut(s) 66
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 5 cut(s) 78, 123, 306, 459, 465
LmnI GCTCC 4 cut(s) 78, 302, 406, 554
Lsp1109I GCAGC 2 cut(s) 50, 456
LweI GCATC 1 cut(s) 605
MaeII ACGT 5 cut(s) 162, 198, 279, 354, 390
MaeIII GTNAC 2 cut(s) 163, 220
MalI GATC 5 cut(s) 80, 125, 308, 461, 467
MbiI CCGCTC 1 cut(s) 297
MboI GATC 5 cut(s) 78, 123, 306, 459, 465
MboII GAAGA 4 cut(s) 73, 163, 193, 499
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 1 cut(s) 570
MmeI TCCRAC 1 cut(s) 559
MnlI CCTC 9 cut(s) 104, 107, 119, 137, 164, 217, 241, 425, 448
MslI CAYNNNNRTG 1 cut(s) 522
MspA1I CMGCKG 1 cut(s) 604
MspI CCGG 5 cut(s) 441, 475, 507, 536, 567
MspR9I CCNGG 4 cut(s) 158, 218, 434, 476
MvaI CCWGG 3 cut(s) 158, 218, 434
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 5 cut(s) 27, 63, 139, 610, 624
NciI CCSGG 1 cut(s) 476
NdeI CATATG 1 cut(s) 629
NdeII GATC 5 cut(s) 78, 123, 306, 459, 465
NlaIV GGNNCC 3 cut(s) 74, 385, 556
NmuCI GTSAC 2 cut(s) 163, 220
NspV TTCGAA 1 cut(s) 8
PceI AGGCCT 1 cut(s) 437
PcsI WCGNNNNNNNCGW 3 cut(s) 273, 285, 387
PfeI GAWTC 1 cut(s) 51
PfoI TCCNGGA 1 cut(s) 156
PkrI GCNGC 4 cut(s) 20, 42, 65, 446
Psp6I CCWGG 3 cut(s) 156, 216, 432
PspEI GGTNACC 1 cut(s) 220
PspFI CCCAGC 1 cut(s) 300
PspGI CCWGG 3 cut(s) 156, 216, 432
PspN4I GGNNCC 3 cut(s) 74, 385, 556
PspPI GGNCC 2 cut(s) 430, 607
PvuII CAGCTG 1 cut(s) 604
RsaI GTAC 2 cut(s) 515, 599
RsaNI GTAC 2 cut(s) 514, 598
RseI CAYNNNNRTG 1 cut(s) 522
SatI GCNGC 4 cut(s) 19, 41, 64, 445
Sau3AI GATC 5 cut(s) 78, 123, 306, 459, 465
Sau96I GGNCC 2 cut(s) 430, 607
ScrFI CCNGG 4 cut(s) 158, 218, 434, 476
SduI GDGCHC 1 cut(s) 75
SfaNI GCATC 1 cut(s) 605
SfiI GGCCNNNNNGGCC 1 cut(s) 27
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 2 cut(s) 430, 607
SmiMI CAYNNNNRTG 1 cut(s) 522
Sse9I AATT 1 cut(s) 570
SseBI AGGCCT 1 cut(s) 437
SsiI CCGC 8 cut(s) 18, 40, 55, 68, 145, 235, 295, 329
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 4 cut(s) 156, 216, 432, 474
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 2 cut(s) 169, 190
TaiI ACGT 5 cut(s) 165, 201, 282, 357, 393
TaqI TCGA 3 cut(s) 8, 276, 468
TasI AATT 1 cut(s) 570
TauI GCSGC 2 cut(s) 21, 43
TfiI GAWTC 1 cut(s) 51
TscAI CASTG 1 cut(s) 65
TseFI GTSAC 2 cut(s) 163, 220
TseI GCWGC 2 cut(s) 63, 444
Tsp45I GTSAC 2 cut(s) 163, 220
TspDTI ATGAA 4 cut(s) 79, 139, 490, 532
TspRI CASTG 1 cut(s) 65
VpaK11BI GGWCC 2 cut(s) 430, 607
XmiI GTMKAC 3 cut(s) 282, 291, 378
ZraI GACGTC 3 cut(s) 163, 280, 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.