FvH4_6g20480

deoxyribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009395
Physical Location & Seq
Forward (+)
14107887 .. 14111788
3902 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20480.t2

Sequence Viewer

Length: 960 bp
ATGGCGACAATCCGCATGATAGATATAGCCGTTAACTTCACAGATGGAATGTTCAAAGGCCTCTACAATGGGAAGCAGTGCCACGTCTCCGATATCGCAACCGTTCTCAGCCGCGCTTGGGCCGCCGGCGTCGACCGCATCATCGTGACTGGTGGATCACTTGAGGAATCAAAGGAAGCTCTCACAATTGCTCAAACTGATGCAAGGCTTTTTTGTACTGTGGGTGTGCACCCAACTAGATGCAAGGAATTTGATGAGAGCGGGGATCCAGAGAAGCATTTCCAGGCGCTTTTTTCGTTGGCCAAAGAGGGAATACAGAAAGGGAAGGTGGTTGCAATTGGTGAGTGTGGACTGGACTATGACAGGGTTCAGTTTTGCCCTCCGGAGATTCAAAAGAAGTATTTTGAGAAGCAGTTTGAATTGGCACATGCCACTAAGCTGCCCATGTTTCTGCATATGCGTGCAGCTGCTCAAGATTTCTGTGAAATTGTAGAAAGAAACAGGGGCAGATTCACTGCTGGGGTGGCCCATTCATTTACTGGTAGTGCAGATGATTGTGATAAACTTCTTTCCATCAACAACATGTATATAGGTGTGAATGGTTGTTCTTTGAAGACAACCGAGAATCTTGATGTTGTAAGAGGCATTCCTATTGAGAGAATGATGATTGAAACAGATTCTCCATATTGTGGAATCAAGAACAGTCATGCTGGGATTAAGTTTGTGAAGTCCACATGGCCTTCTAAGAAGAAAGAGAAGTATGATCAAGAGTGCATTGTTAAAGATCGCAATGAACCTTGCTTGGTGCGGCAAGTTCTTGAGGTGATAGCTGGTTGTAAAGGAATCAGTGATGTAAGTCAGCTGAGCAGGACATTATACCAGAACACTTGCAGGGTTTTCTTTCCTCAAGACTTAGATTCTGCAGCAGATTCTTTACTTGCTGGGCACGACCCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TatD_DNase

Protein Analysis

320

Amino Acids

35.45

Weight (kDa)

6.26

Isoelectric Point (pI)

26.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TatD_DNase PF01026 30 - 300 4.6e-53 TatD related DNase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 689
AccBSI CCGCTC 1 cut(s) 261
AccI GTMKAC 1 cut(s) 132
AccII CGCG 1 cut(s) 114
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 6 cut(s) 13, 112, 123, 136, 261, 808
AclWI GGATC 3 cut(s) 163, 260, 273
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 1 cut(s) 248
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 1 cut(s) 217
AfiI CCNNNNNNNGG 2 cut(s) 118, 689
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 5 cut(s) 55, 392, 419, 613, 671
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 282
AluBI AGCT 5 cut(s) 179, 439, 467, 830, 862
AluI AGCT 5 cut(s) 179, 439, 467, 830, 862
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 91
Alw44I GTGCAC 1 cut(s) 227
AlwI GGATC 3 cut(s) 163, 260, 273
Aor13HI TCCGGA 1 cut(s) 382
AoxI GGCC 5 cut(s) 58, 120, 300, 525, 737
ApaLI GTGCAC 1 cut(s) 227
ApeKI GCWGC 4 cut(s) 439, 464, 467, 923
ApoI RAATTY 1 cut(s) 248
Asp700I GAANNNNTTC 1 cut(s) 278
AspLEI GCGC 2 cut(s) 116, 289
AspS9I GGNCC 2 cut(s) 120, 526
AsuHPI GGTGA 2 cut(s) 353, 835
BaeGI GKGCMC 2 cut(s) 231, 948
BalI TGGCCA 1 cut(s) 302
BamHI GGATCC 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 426, 454, 476, 935
BccI CCATC 2 cut(s) 38, 581
BceAI ACGGC 1 cut(s) 14
BciT130I CCWGG 1 cut(s) 284
BclI TGATCA 1 cut(s) 763
BcoDI GTCTC 1 cut(s) 91
BfaI CTAG 1 cut(s) 237
BfmI CTRYAG 1 cut(s) 921
BfoI RGCGCY 1 cut(s) 290
BisI GCNGC 7 cut(s) 112, 123, 440, 465, 468, 809, 924
BlpI GCTNAGC 1 cut(s) 863
BlsI GCNGC 7 cut(s) 113, 124, 441, 466, 469, 810, 925
Bme1390I CCNGG 1 cut(s) 284
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 2 cut(s) 120, 526
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 284
BmsI GCATC 3 cut(s) 147, 190, 230
BpiI GAAGAC 1 cut(s) 620
Bpu1102I GCTNAGC 1 cut(s) 863
BpuEI CTTGAG 4 cut(s) 182, 456, 839, 891
BsaHI GRCGYC 1 cut(s) 129
BsaWI WCCGGW 1 cut(s) 382
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 689
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 3 cut(s) 154, 357, 544
Bse3DI GCAATG 1 cut(s) 796
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 1 cut(s) 284
BseLI CCNNNNNNNGG 2 cut(s) 118, 689
BseMI GCAATG 1 cut(s) 796
BseMII CTCAG 2 cut(s) 121, 854
BseNI ACTGG 3 cut(s) 154, 357, 544
BseSI GKGCMC 2 cut(s) 231, 948
BseXI GCAGC 4 cut(s) 426, 454, 476, 935
BseYI CCCAGC 3 cut(s) 518, 710, 941
BsgI GTGCAG 2 cut(s) 483, 567
Bsh1236I CGCG 1 cut(s) 114
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 5 cut(s) 60, 122, 302, 527, 739
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 2 cut(s) 126, 383
BslI CCNNNNNNNGG 2 cut(s) 118, 689
BsmAI GTCTC 1 cut(s) 91
BsmBI CGTCTC 1 cut(s) 91
BsmI GAATGC 1 cut(s) 645
BsnI GGCC 5 cut(s) 60, 122, 302, 527, 739
Bsp1286I GDGCHC 2 cut(s) 231, 948
Bsp13I TCCGGA 1 cut(s) 382
Bsp143I GATC 4 cut(s) 155, 265, 763, 784
Bsp1720I GCTNAGC 1 cut(s) 863
BspACI CCGC 6 cut(s) 13, 112, 123, 136, 261, 808
BspANI GGCC 5 cut(s) 60, 122, 302, 527, 739
BspCNI CTCAG 2 cut(s) 120, 855
BspEI TCCGGA 1 cut(s) 382
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 925
BspPI GGATC 3 cut(s) 163, 260, 273
BsrBI CCGCTC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 796
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 3 cut(s) 154, 357, 544
BssAI RCCGGY 1 cut(s) 125
BssMI GATC 4 cut(s) 155, 265, 763, 784
BssNI GRCGYC 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 3 cut(s) 103, 220, 704
BstACI GRCGYC 1 cut(s) 129
BstC8I GCNNGC 2 cut(s) 127, 462
BstDEI CTNAG 6 cut(s) 107, 435, 744, 863, 913, 954
BstFNI CGCG 1 cut(s) 114
BstH2I RGCGCY 1 cut(s) 290
BstHHI GCGC 2 cut(s) 116, 289
BstKTI GATC 4 cut(s) 158, 268, 766, 787
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 4 cut(s) 155, 265, 763, 784
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 3 cut(s) 122, 135, 524
BstNI CCWGG 1 cut(s) 284
BstNSI RCATGY 2 cut(s) 431, 586
BstSCI CCNGG 1 cut(s) 282
BstSFI CTRYAG 1 cut(s) 921
BstSLI GKGCMC 2 cut(s) 231, 948
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 4 cut(s) 426, 454, 476, 935
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 5 cut(s) 60, 122, 302, 527, 739
BtrI CACGTC 1 cut(s) 85
BtsI GCAGTG 2 cut(s) 83, 513
BtsIMutI CAGTG 3 cut(s) 83, 513, 853
Cac8I GCNNGC 2 cut(s) 127, 462
CfoI GCGC 2 cut(s) 116, 289
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 120, 526
CseI GACGC 1 cut(s) 118
Csp6I GTAC 1 cut(s) 216
CviAII CATG 6 cut(s) 16, 428, 445, 583, 707, 735
CviQI GTAC 1 cut(s) 216
DdeI CTNAG 6 cut(s) 107, 435, 744, 863, 913, 954
DpnI GATC 4 cut(s) 157, 267, 765, 786
DpnII GATC 4 cut(s) 155, 265, 763, 784
EaeI YGGCCR 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 60
Eco32I GATATC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 282
EcoRV GATATC 1 cut(s) 94
Esp3I CGTCTC 1 cut(s) 91
FaeI CATG 6 cut(s) 19, 431, 448, 586, 710, 738
FatI CATG 6 cut(s) 15, 427, 444, 582, 706, 734
FauI CCCGC 1 cut(s) 254
FauNDI CATATG 1 cut(s) 456
FbaI TGATCA 1 cut(s) 763
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 7 cut(s) 112, 123, 440, 465, 468, 809, 924
Fsp4HI GCNGC 7 cut(s) 112, 123, 440, 465, 468, 809, 924
FspBI CTAG 1 cut(s) 237
GlaI GCGC 2 cut(s) 115, 288
GluI GCNGC 7 cut(s) 112, 123, 440, 465, 468, 809, 924
GsaI CCCAGC 3 cut(s) 522, 714, 945
HaeII RGCGCY 1 cut(s) 290
HaeIII GGCC 5 cut(s) 60, 122, 302, 527, 739
HapII CCGG 2 cut(s) 126, 383
HgaI GACGC 1 cut(s) 118
HhaI GCGC 2 cut(s) 116, 289
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 6 cut(s) 19, 431, 448, 586, 710, 738
Hin6I GCGC 2 cut(s) 114, 287
HinP1I GCGC 2 cut(s) 114, 287
HincII GTYRAC 2 cut(s) 34, 133
HindII GTYRAC 2 cut(s) 34, 133
HinfI GANTC 9 cut(s) 167, 388, 510, 625, 677, 693, 843, 917, 929
HpaI GTTAAC 1 cut(s) 34
HpaII CCGG 2 cut(s) 126, 383
HphI GGTGA 2 cut(s) 353, 835
Hpy166II GTNNAC 5 cut(s) 34, 133, 229, 350, 732
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 9 cut(s) 145, 269, 383, 473, 629, 697, 767, 818, 908
Hpy8I GTNNAC 5 cut(s) 34, 133, 229, 350, 732
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 2 cut(s) 319, 750
HpyCH4III ACNGT 3 cut(s) 103, 220, 704
HpyCH4IV ACGT 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 135, 524
HpyF3I CTNAG 6 cut(s) 107, 435, 744, 863, 913, 954
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 6 cut(s) 19, 431, 448, 586, 710, 738
HspAI GCGC 2 cut(s) 114, 287
Kpn2I TCCGGA 1 cut(s) 382
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 763
KspAI GTTAAC 1 cut(s) 34
Kzo9I GATC 4 cut(s) 155, 265, 763, 784
Lsp1109I GCAGC 4 cut(s) 426, 454, 476, 935
LweI GCATC 3 cut(s) 147, 190, 230
MaeI CTAG 1 cut(s) 237
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 145
MalI GATC 4 cut(s) 157, 267, 765, 786
MbiI CCGCTC 1 cut(s) 261
MboI GATC 4 cut(s) 155, 265, 763, 784
MboII GAAGA 2 cut(s) 625, 760
MfeI CAATTG 2 cut(s) 186, 336
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 2 cut(s) 231, 948
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 5 cut(s) 186, 248, 336, 419, 486
MluNI TGGCCA 1 cut(s) 302
MnlI CCTC 7 cut(s) 71, 157, 301, 390, 635, 814, 915
Mox20I TGGCCA 1 cut(s) 302
MreI CGCCGGCG 1 cut(s) 125
MroI TCCGGA 1 cut(s) 382
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 1 cut(s) 278
MscI TGGCCA 1 cut(s) 302
MseI TTAA 3 cut(s) 33, 717, 780
MslI CAYNNNNRTG 2 cut(s) 143, 459
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 2 cut(s) 467, 862
MspI CCGG 2 cut(s) 126, 383
MspR9I CCNGG 1 cut(s) 284
MunI CAATTG 2 cut(s) 186, 336
Mva1269I GAATGC 1 cut(s) 645
MvaI CCWGG 1 cut(s) 284
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 3 cut(s) 122, 135, 524
NaeI GCCGGC 1 cut(s) 127
NdeI CATATG 1 cut(s) 456
NdeII GATC 4 cut(s) 155, 265, 763, 784
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 6 cut(s) 19, 431, 448, 586, 710, 738
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 145
NspI RCATGY 2 cut(s) 431, 586
PceI AGGCCT 1 cut(s) 60
PciI ACATGT 1 cut(s) 582
PctI GAATGC 1 cut(s) 645
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 9 cut(s) 167, 388, 510, 625, 677, 693, 843, 917, 929
PflMI CCANNNNNTGG 1 cut(s) 689
PkrI GCNGC 7 cut(s) 113, 124, 441, 466, 469, 810, 925
PscI ACATGT 1 cut(s) 582
Psp6I CCWGG 1 cut(s) 282
PspFI CCCAGC 3 cut(s) 518, 710, 941
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 2 cut(s) 120, 526
PstI CTGCAG 1 cut(s) 925
PsuI RGATCY 1 cut(s) 265
PvuII CAGCTG 2 cut(s) 467, 862
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
RseI CAYNNNNRTG 2 cut(s) 143, 459
SalI GTCGAC 1 cut(s) 131
SaqAI TTAA 3 cut(s) 33, 717, 780
SatI GCNGC 7 cut(s) 112, 123, 440, 465, 468, 809, 924
Sau3AI GATC 4 cut(s) 155, 265, 763, 784
Sau96I GGNCC 2 cut(s) 120, 526
ScrFI CCNGG 1 cut(s) 284
SduI GDGCHC 2 cut(s) 231, 948
SfaNI GCATC 3 cut(s) 147, 190, 230
SfcI CTRYAG 1 cut(s) 921
SgrAI CRCCGGYG 1 cut(s) 125
SmiMI CAYNNNNRTG 2 cut(s) 143, 459
SmlI CTYRAG 4 cut(s) 161, 471, 818, 906
SmoI CTYRAG 4 cut(s) 161, 471, 818, 906
Sse9I AATT 5 cut(s) 186, 248, 336, 419, 486
SseBI AGGCCT 1 cut(s) 60
SsiI CCGC 6 cut(s) 13, 112, 123, 136, 261, 808
SspMI CTAG 1 cut(s) 237
StuI AGGCCT 1 cut(s) 60
StyD4I CCNGG 1 cut(s) 282
TaaI ACNGT 3 cut(s) 103, 220, 704
TaiI ACGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 132
TasI AATT 5 cut(s) 186, 248, 336, 419, 486
TatI WGTACW 1 cut(s) 215
TauI GCSGC 3 cut(s) 114, 125, 811
TfiI GAWTC 9 cut(s) 167, 388, 510, 625, 677, 693, 843, 917, 929
Tru1I TTAA 3 cut(s) 33, 717, 780
Tru9I TTAA 3 cut(s) 33, 717, 780
TscAI CASTG 3 cut(s) 83, 520, 853
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 4 cut(s) 439, 464, 467, 923
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 2 cut(s) 522, 807
TspRI CASTG 3 cut(s) 83, 520, 853
Van91I CCANNNNNTGG 1 cut(s) 689
VneI GTGCAC 1 cut(s) 227
XapI RAATTY 1 cut(s) 248
XceI RCATGY 2 cut(s) 431, 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.