Prupe.7G093300_v2.0.a1

deoxyribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0009395
Physical Location & Seq
Reverse (-)
12575078 .. 12579128
4051 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G093300.6

Sequence Viewer

Length: 993 bp
ATGGCGACAGTCCGCATGATAGATATAGCCGTCAACTTCACAGATGGCATGTTCAAAGGAATCTACAATGGGAAGCAGTGCCACGTCTCGGATATAGCTACTGTTTTGAGCAGGGCTTGGACTGCCGGCGTCGACCGCATCATCGTCACTGGTGGATCACTTGAGGAATCAAAGGAAGCTCTAACAATTGCAGAAACCGATGCAAGGCTTTTTTGTACAGTTGGTGTGCACCCAACTAGATGCAAGGAATTCGAAGAGAGCGGGGATCCAGATAAGCATTTTCAGGCGCTTTTGTCATTGGCCAAAGAGGGAATAGAGAAAGGAAAGGTGGTTGCAATTGGTGAATGTGGATTGGACTATGATAGGCTTCAGTTTTGCCCAGCAGAGATTCAAAAGAAGTATTTTGAGAAGCAGTTTGAATTAGCACATGCCATGAAGCTGCCCATGTTTCTGCATATGCGTGCAGCTGCTCCAGATTTTTGTGAAATTGTGGAGAGAAATAGGAGCAGATTCAGCGCAGGGGTGGCCCATTCATTTACTGGTAGTGCAGAAGATCGTGATAAACTTCTTTCCATCAGCAACATGTATATAGGTGTAAATGGTTGCTCTCTGAAGACAGCTGAGAATCTTGATGTTGTGAGGAGCATTCCTATTGAGAGGATGATGATTGAGACTGATTCTCCATATTGTGGAATCAAGAACACAAGTGCCGGGATTAAATTTGTAAAGTCCACTTGGCCTTCTAAGAAGAAAGAGAAGTACGATCAAGAGTGCATTGTTAAAGACCGCAATGAACCGTGTTTGGTTCGGCAAGTTCTAGAGGTGGTAGCTGGTTGCAAAGGAATCAGTGAAATAGGTCAGCTGAGCAGGACACTATACCACAACACTTGCCGGACTTGGATTCTGCAGCAGATTCTCTACTTGCTGGTCATGACCCTCAGTGATAAAACCAAATTCCTTCCCGCCGCAGGTTATTCAGTTCCTCTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TatD_DNase

Protein Analysis

331

Amino Acids

36.8

Weight (kDa)

7.5

Isoelectric Point (pI)

32.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 959
AccB7I CCANNNNNTGG 1 cut(s) 689
AccBSI CCGCTC 1 cut(s) 261
AccI GTMKAC 1 cut(s) 132
AciI CCGC 6 cut(s) 13, 136, 261, 787, 963, 966
AclWI GGATC 3 cut(s) 163, 260, 273
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 3 cut(s) 248, 719, 953
AcuI CTGAAG 2 cut(s) 353, 632
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 2 cut(s) 217, 761
AfiI CCNNNNNNNGG 4 cut(s) 88, 204, 689, 968
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 3 cut(s) 55, 392, 419
AjiI CACGTC 1 cut(s) 85
AluBI AGCT 7 cut(s) 98, 179, 439, 467, 620, 830, 862
AluI AGCT 7 cut(s) 98, 179, 439, 467, 620, 830, 862
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 2 cut(s) 91, 665
Alw44I GTGCAC 1 cut(s) 227
AlwI GGATC 3 cut(s) 163, 260, 273
AoxI GGCC 3 cut(s) 300, 525, 737
ApaLI GTGCAC 1 cut(s) 227
ApeKI GCWGC 4 cut(s) 439, 464, 467, 907
ApoI RAATTY 3 cut(s) 248, 719, 953
AspLEI GCGC 2 cut(s) 289, 518
AspS9I GGNCC 1 cut(s) 526
AsuC2I CCSGG 1 cut(s) 712
AsuHPI GGTGA 1 cut(s) 353
AsuII TTCGAA 1 cut(s) 252
BaeGI GKGCMC 1 cut(s) 231
BalI TGGCCA 1 cut(s) 302
BamHI GGATCC 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 426, 454, 476, 919
BccI CCATC 2 cut(s) 38, 581
BceAI ACGGC 1 cut(s) 14
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BcnI CCSGG 1 cut(s) 712
BcoDI GTCTC 2 cut(s) 91, 665
BfaI CTAG 2 cut(s) 237, 818
BfmI CTRYAG 1 cut(s) 905
BfoI RGCGCY 1 cut(s) 290
BfuAI ACCTGC 1 cut(s) 959
BisI GCNGC 5 cut(s) 440, 465, 468, 908, 966
BlpI GCTNAGC 1 cut(s) 863
BlsI GCNGC 5 cut(s) 441, 466, 469, 909, 967
Bme1390I CCNGG 1 cut(s) 712
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 526
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 712
BmsI GCATC 3 cut(s) 147, 190, 230
BpiI GAAGAC 1 cut(s) 620
BpmI CTGGAG 1 cut(s) 456
Bpu1102I GCTNAGC 1 cut(s) 863
Bpu14I TTCGAA 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 712
BsaHI GRCGYC 1 cut(s) 129
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 204, 689, 968
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 154, 544
Bse3DI GCAATG 1 cut(s) 796
BseGI GGATG 1 cut(s) 666
BseLI CCNNNNNNNGG 4 cut(s) 88, 204, 689, 968
BseMI GCAATG 1 cut(s) 796
BseMII CTCAG 3 cut(s) 612, 854, 952
BseNI ACTGG 2 cut(s) 154, 544
BseRI GAGGAG 1 cut(s) 655
BseSI GKGCMC 1 cut(s) 231
BseXI GCAGC 4 cut(s) 426, 454, 476, 919
BseYI CCCAGC 1 cut(s) 379
BsgI GTGCAG 2 cut(s) 483, 567
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 3 cut(s) 302, 527, 739
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 3 cut(s) 126, 711, 892
BslI CCNNNNNNNGG 4 cut(s) 88, 204, 689, 968
BsmAI GTCTC 2 cut(s) 91, 665
BsmBI CGTCTC 1 cut(s) 91
BsmI GAATGC 1 cut(s) 645
BsnI GGCC 3 cut(s) 302, 527, 739
Bsp119I TTCGAA 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 231
Bsp1407I TGTACA 1 cut(s) 215
Bsp143I GATC 4 cut(s) 155, 265, 553, 763
Bsp1720I GCTNAGC 1 cut(s) 863
BspACI CCGC 6 cut(s) 13, 136, 261, 787, 963, 966
BspANI GGCC 3 cut(s) 302, 527, 739
BspCNI CTCAG 3 cut(s) 613, 855, 951
BspHI TCATGA 2 cut(s) 930, 989
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 909
BspMI ACCTGC 1 cut(s) 959
BspPI GGATC 3 cut(s) 163, 260, 273
BspT104I TTCGAA 1 cut(s) 252
BsrBI CCGCTC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 796
BsrFI RCCGGY 1 cut(s) 125
BsrGI TGTACA 1 cut(s) 215
BsrI ACTGG 2 cut(s) 154, 544
BssAI RCCGGY 1 cut(s) 125
BssMI GATC 4 cut(s) 155, 265, 553, 763
BssNI GRCGYC 1 cut(s) 129
Bst4CI ACNGT 4 cut(s) 10, 103, 220, 798
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 1 cut(s) 129
BstAUI TGTACA 1 cut(s) 215
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 2 cut(s) 127, 462
BstDEI CTNAG 4 cut(s) 621, 744, 863, 938
BstF5I GGATG 1 cut(s) 666
BstH2I RGCGCY 1 cut(s) 290
BstHHI GCGC 2 cut(s) 289, 518
BstKTI GATC 4 cut(s) 158, 268, 556, 766
BstMAI GTCTC 2 cut(s) 91, 665
BstMBI GATC 4 cut(s) 155, 265, 553, 763
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 4 cut(s) 122, 135, 513, 524
BstNSI RCATGY 3 cut(s) 52, 431, 586
BstSCI CCNGG 1 cut(s) 710
BstSFI CTRYAG 1 cut(s) 905
BstSLI GKGCMC 1 cut(s) 231
BstV1I GCAGC 4 cut(s) 426, 454, 476, 919
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 3 cut(s) 302, 527, 739
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 666
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 4 cut(s) 83, 147, 853, 946
BveI ACCTGC 1 cut(s) 959
Cac8I GCNNGC 2 cut(s) 127, 462
CciI TCATGA 2 cut(s) 930, 989
CfoI GCGC 2 cut(s) 289, 518
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 526
CseI GACGC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 216, 760
CspCI CAANNNNNGTGG 2 cut(s) 869, 904
CviAII CATG 8 cut(s) 16, 49, 428, 433, 445, 583, 931, 990
CviQI GTAC 2 cut(s) 216, 760
DdeI CTNAG 4 cut(s) 621, 744, 863, 938
DpnI GATC 4 cut(s) 157, 267, 555, 765
DpnII GATC 4 cut(s) 155, 265, 553, 763
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco57I CTGAAG 2 cut(s) 353, 632
EcoRI GAATTC 1 cut(s) 248
Esp3I CGTCTC 1 cut(s) 91
FaeI CATG 8 cut(s) 19, 52, 431, 436, 448, 586, 934, 993
FatI CATG 8 cut(s) 15, 48, 427, 432, 444, 582, 930, 989
FauI CCCGC 2 cut(s) 254, 970
FauNDI CATATG 1 cut(s) 456
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 5 cut(s) 440, 465, 468, 908, 966
FokI GGATG 1 cut(s) 673
Fsp4HI GCNGC 5 cut(s) 440, 465, 468, 908, 966
FspBI CTAG 2 cut(s) 237, 818
GlaI GCGC 2 cut(s) 288, 517
GluI GCNGC 5 cut(s) 440, 465, 468, 908, 966
GsaI CCCAGC 1 cut(s) 383
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 290
HaeIII GGCC 3 cut(s) 302, 527, 739
HapII CCGG 3 cut(s) 126, 711, 892
HgaI GACGC 1 cut(s) 118
HhaI GCGC 2 cut(s) 289, 518
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 8 cut(s) 19, 52, 431, 436, 448, 586, 934, 993
Hin6I GCGC 2 cut(s) 287, 516
HinP1I GCGC 2 cut(s) 287, 516
HincII GTYRAC 2 cut(s) 34, 133
HindII GTYRAC 2 cut(s) 34, 133
HpaII CCGG 3 cut(s) 126, 711, 892
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 4 cut(s) 34, 133, 229, 732
Hpy188I TCNGA 2 cut(s) 91, 612
Hpy188III TCNNGA 9 cut(s) 269, 473, 557, 629, 697, 767, 818, 931, 990
Hpy8I GTNNAC 4 cut(s) 34, 133, 229, 732
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 2 cut(s) 750, 968
HpyCH4III ACNGT 4 cut(s) 10, 103, 220, 798
HpyCH4IV ACGT 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 135, 513, 524
HpyF3I CTNAG 4 cut(s) 621, 744, 863, 938
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 8 cut(s) 19, 52, 431, 436, 448, 586, 934, 993
HspAI GCGC 2 cut(s) 287, 516
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Kzo9I GATC 4 cut(s) 155, 265, 553, 763
LmnI GCTCC 3 cut(s) 475, 504, 642
Lsp1109I GCAGC 4 cut(s) 426, 454, 476, 919
LweI GCATC 3 cut(s) 147, 190, 230
MaeI CTAG 2 cut(s) 237, 818
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 145
MalI GATC 4 cut(s) 157, 267, 555, 765
MbiI CCGCTC 1 cut(s) 261
MboI GATC 4 cut(s) 155, 265, 553, 763
MboII GAAGA 4 cut(s) 266, 563, 625, 760
MfeI CAATTG 2 cut(s) 186, 336
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 231
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 7 cut(s) 186, 248, 336, 419, 486, 719, 953
MluNI TGGCCA 1 cut(s) 302
MnlI CCTC 7 cut(s) 157, 301, 633, 651, 814, 947, 993
Mox20I TGGCCA 1 cut(s) 302
MroNI GCCGGC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 302
MseI TTAA 2 cut(s) 717, 780
MslI CAYNNNNRTG 1 cut(s) 459
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 3 cut(s) 467, 620, 862
MspI CCGG 3 cut(s) 126, 711, 892
MspR9I CCNGG 1 cut(s) 712
MunI CAATTG 2 cut(s) 186, 336
Mva1269I GAATGC 1 cut(s) 645
MwoI GCNNNNNNNGC 4 cut(s) 122, 135, 513, 524
NaeI GCCGGC 1 cut(s) 127
NciI CCSGG 1 cut(s) 712
NdeI CATATG 1 cut(s) 456
NdeII GATC 4 cut(s) 155, 265, 553, 763
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 8 cut(s) 19, 52, 431, 436, 448, 586, 934, 993
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 145
NspI RCATGY 3 cut(s) 52, 431, 586
NspV TTCGAA 1 cut(s) 252
PagI TCATGA 2 cut(s) 930, 989
PciI ACATGT 1 cut(s) 582
PctI GAATGC 1 cut(s) 645
PdiI GCCGGC 1 cut(s) 127
PflMI CCANNNNNTGG 1 cut(s) 689
PkrI GCNGC 5 cut(s) 441, 466, 469, 909, 967
PscI ACATGT 1 cut(s) 582
PspFI CCCAGC 1 cut(s) 379
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 1 cut(s) 526
PstI CTGCAG 1 cut(s) 909
PsuI RGATCY 1 cut(s) 265
PvuII CAGCTG 3 cut(s) 467, 620, 862
RsaI GTAC 2 cut(s) 217, 761
RsaNI GTAC 2 cut(s) 216, 760
RseI CAYNNNNRTG 1 cut(s) 459
SalI GTCGAC 1 cut(s) 131
SaqAI TTAA 2 cut(s) 717, 780
SatI GCNGC 5 cut(s) 440, 465, 468, 908, 966
Sau3AI GATC 4 cut(s) 155, 265, 553, 763
Sau96I GGNCC 1 cut(s) 526
ScrFI CCNGG 1 cut(s) 712
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 3 cut(s) 147, 190, 230
SfcI CTRYAG 1 cut(s) 905
SfuI TTCGAA 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 7 cut(s) 186, 248, 336, 419, 486, 719, 953
SsiI CCGC 6 cut(s) 13, 136, 261, 787, 963, 966
SspMI CTAG 2 cut(s) 237, 818
StyD4I CCNGG 1 cut(s) 710
TaaI ACNGT 4 cut(s) 10, 103, 220, 798
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 132, 252
TasI AATT 7 cut(s) 186, 248, 336, 419, 486, 719, 953
TatI WGTACW 1 cut(s) 215
TauI GCSGC 1 cut(s) 968
Tru1I TTAA 2 cut(s) 717, 780
Tru9I TTAA 2 cut(s) 717, 780
TscAI CASTG 4 cut(s) 83, 154, 853, 946
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 4 cut(s) 439, 464, 467, 907
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 4 cut(s) 449, 522, 807, 978
TspRI CASTG 4 cut(s) 83, 154, 853, 946
Van91I CCANNNNNTGG 1 cut(s) 689
VneI GTGCAC 1 cut(s) 227
XapI RAATTY 3 cut(s) 248, 719, 953
XbaI TCTAGA 1 cut(s) 817
XceI RCATGY 3 cut(s) 52, 431, 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 2 cut(s) 237, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.