RLG00000023777

deoxyribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009395
Physical Location & Seq
Reverse (-)
28076774 .. 28080624
3851 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023777

Sequence Viewer

Length: 912 bp
ATGTTCAAAGGACTCTACAATGGAAAGCAGTGCCACGTCTCCGATATACCCACCGTTTTGACCCGGGCTTGGGCTGCCGGCGTCCACCGCATCATCGTCACTGGTGGATCACTTGAGGAATCAAAGGAAGCTTTAACAATTGCACAAACTGATGCAAGGCTTTTTTGTACTGTGGGTGTGCACCCAACTAGATGCAAGGAATTTGATGAGAGCGGGGATCCAGATAAGCATTTTCAGGCGCTTTTGTCATTGGCCAGAGAGGGGATAGAGAAAGGGAAGGTGGTTGCAATTGGTGAGTGTGGACTGGACTATGACAGGCTTCAGTTTTGCCCTCCGGAAATTCAAAAGAAGTATTTTGAGAAGCAGTTTGAATTGGCACATGCCACCAAGCTGCCCATGTTTCTGCATATGCGTGCAGCTGCTCAAGATTTCTATGAAATTGTAGAAAGAAATAGGAACAGATTCACTGCTGGGGTGGCCCATTCATTTACTGGTAGTTCAGACGATTGTGATAAGCTTCTTTCCATCTGCAACATGTATATAGGTGTGAATGGTTGCTCTTTGAAGACAACTGAGAATCTTGATGTTGTGAGGGGCATTCCTATCGAGAGAATGATGATTGAAACAGATTCTCCATATTGTGGAATCAAGAACAGTCATGCTGGGGTTAAATTTGTGAAGTCCACATGGCCTTCTAAGAAGAAAGAGAAGTATGATGAAGAGTGCATTGTTAAAGACCGCAATGAACCTTGTTTGGTTCAGCAAGTTCTTGAGGTGATAGCTGGTTGTAAAGGAATCAGTGATGTAGGTCAGCTGAGCAGGACACTGTACCAGAACACTTGCAGGGTTTTCTTTCCTCAAGACTTGGATTCTGCTGCAGATTCTTTACTTGCTGGGCACGACCCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TatD_DNase

Protein Analysis

304

Amino Acids

33.81

Weight (kDa)

6.16

Isoelectric Point (pI)

31.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TatD_DNase PF01026 14 - 284 2.9e-52 TatD related DNase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 641
AccBSI CCGCTC 1 cut(s) 213
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 3 cut(s) 88, 213, 739
AclWI GGATC 3 cut(s) 115, 212, 225
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 3 cut(s) 200, 339, 671
AcuI CTGAAG 1 cut(s) 305
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 2 cut(s) 169, 830
AfiI CCNNNNNNNGG 4 cut(s) 69, 70, 261, 641
AflIII ACRYGT 1 cut(s) 534
AgsI TTSAA 5 cut(s) 7, 344, 371, 565, 623
AjiI CACGTC 1 cut(s) 37
AluBI AGCT 6 cut(s) 131, 391, 419, 517, 782, 814
AluI AGCT 6 cut(s) 131, 391, 419, 517, 782, 814
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 1 cut(s) 43
Alw44I GTGCAC 1 cut(s) 179
AlwI GGATC 3 cut(s) 115, 212, 225
Ama87I CYCGRG 1 cut(s) 63
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 3 cut(s) 252, 477, 689
ApaLI GTGCAC 1 cut(s) 179
ApeKI GCWGC 5 cut(s) 74, 391, 416, 419, 875
ApoI RAATTY 3 cut(s) 200, 339, 671
Asp700I GAANNNNTTC 1 cut(s) 461
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 478
AsuC2I CCSGG 2 cut(s) 64, 65
AsuHPI GGTGA 2 cut(s) 305, 787
AvaI CYCGRG 1 cut(s) 63
BaeGI GKGCMC 2 cut(s) 183, 900
BalI TGGCCA 1 cut(s) 254
BamHI GGATCC 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 572
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 5 cut(s) 61, 378, 406, 428, 862
BccI CCATC 1 cut(s) 533
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BcnI CCSGG 2 cut(s) 64, 65
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 876
BfoI RGCGCY 1 cut(s) 242
BisI GCNGC 5 cut(s) 75, 392, 417, 420, 876
BlpI GCTNAGC 1 cut(s) 815
BlsI GCNGC 5 cut(s) 76, 393, 418, 421, 877
Bme1390I CCNGG 2 cut(s) 64, 65
BmeT110I CYCGRG 1 cut(s) 63
BmgBI CACGTC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 478
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 2 cut(s) 64, 65
BmsI GCATC 3 cut(s) 99, 142, 182
BpiI GAAGAC 1 cut(s) 572
Bpu1102I GCTNAGC 1 cut(s) 815
BpuEI CTTGAG 4 cut(s) 134, 408, 791, 843
BpuMI CCSGG 2 cut(s) 64, 65
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 63
BsaWI WCCGGW 1 cut(s) 334
BsaXI ACNNNNNCTCC 2 cut(s) 616, 646
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 70, 261, 641
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 3 cut(s) 106, 309, 496
Bse3DI GCAATG 1 cut(s) 748
BseAI TCCGGA 1 cut(s) 334
BseDI CCNNGG 1 cut(s) 63
BseLI CCNNNNNNNGG 4 cut(s) 69, 70, 261, 641
BseMI GCAATG 1 cut(s) 748
BseMII CTCAG 2 cut(s) 564, 806
BseNI ACTGG 3 cut(s) 106, 309, 496
BseSI GKGCMC 2 cut(s) 183, 900
BseXI GCAGC 5 cut(s) 61, 378, 406, 428, 862
BseYI CCCAGC 3 cut(s) 470, 662, 893
BsgI GTGCAG 1 cut(s) 435
BshFI GGCC 3 cut(s) 254, 479, 691
BsiHKAI GWGCWC 1 cut(s) 183
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 3 cut(s) 64, 78, 335
BslI CCNNNNNNNGG 4 cut(s) 69, 70, 261, 641
BsmAI GTCTC 1 cut(s) 43
BsmBI CGTCTC 1 cut(s) 43
BsmI GAATGC 1 cut(s) 597
BsnI GGCC 3 cut(s) 254, 479, 691
BsoBI CYCGRG 1 cut(s) 63
Bsp1286I GDGCHC 2 cut(s) 183, 900
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 2 cut(s) 107, 217
Bsp1720I GCTNAGC 1 cut(s) 815
BspACI CCGC 3 cut(s) 88, 213, 739
BspANI GGCC 3 cut(s) 254, 479, 691
BspCNI CTCAG 2 cut(s) 565, 807
BspEI TCCGGA 1 cut(s) 334
BspLI GGNNCC 1 cut(s) 219
BspMAI CTGCAG 1 cut(s) 880
BspPI GGATC 3 cut(s) 115, 212, 225
BsrBI CCGCTC 1 cut(s) 213
BsrDI GCAATG 1 cut(s) 748
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 3 cut(s) 106, 309, 496
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 63
BssMI GATC 2 cut(s) 107, 217
BssNI GRCGYC 1 cut(s) 81
Bst4CI ACNGT 4 cut(s) 55, 172, 656, 828
Bst6I CTCTTC 1 cut(s) 714
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 79, 414
BstDEI CTNAG 4 cut(s) 573, 696, 815, 906
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 2 cut(s) 110, 220
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 2 cut(s) 107, 217
BstMWI GCNNNNNNNGC 3 cut(s) 74, 87, 476
BstNSI RCATGY 2 cut(s) 383, 538
BstSCI CCNGG 2 cut(s) 62, 63
BstSFI CTRYAG 1 cut(s) 876
BstSLI GKGCMC 2 cut(s) 183, 900
BstV1I GCAGC 5 cut(s) 61, 378, 406, 428, 862
BstV2I GAAGAC 1 cut(s) 572
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 3 cut(s) 254, 479, 691
BtrI CACGTC 1 cut(s) 37
BtsI GCAGTG 2 cut(s) 35, 465
BtsIMutI CAGTG 5 cut(s) 35, 99, 465, 805, 824
Cac8I GCNNGC 2 cut(s) 79, 414
CfoI GCGC 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 478
Cfr9I CCCGGG 1 cut(s) 63
CseI GACGC 1 cut(s) 70
Csp6I GTAC 2 cut(s) 168, 829
CviAII CATG 5 cut(s) 380, 397, 535, 659, 687
CviQI GTAC 2 cut(s) 168, 829
DdeI CTNAG 4 cut(s) 573, 696, 815, 906
DpnI GATC 2 cut(s) 109, 219
DpnII GATC 2 cut(s) 107, 217
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
Eco57I CTGAAG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 63
Esp3I CGTCTC 1 cut(s) 43
FaeI CATG 5 cut(s) 383, 400, 538, 662, 690
FatI CATG 5 cut(s) 379, 396, 534, 658, 686
FauI CCCGC 1 cut(s) 206
FauNDI CATATG 1 cut(s) 408
Fnu4HI GCNGC 5 cut(s) 75, 392, 417, 420, 876
Fsp4HI GCNGC 5 cut(s) 75, 392, 417, 420, 876
FspBI CTAG 1 cut(s) 189
GlaI GCGC 1 cut(s) 240
GluI GCNGC 5 cut(s) 75, 392, 417, 420, 876
GsaI CCCAGC 3 cut(s) 474, 666, 897
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 3 cut(s) 254, 479, 691
HapII CCGG 3 cut(s) 64, 78, 335
HgaI GACGC 1 cut(s) 70
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 5 cut(s) 383, 400, 538, 662, 690
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HindIII AAGCTT 2 cut(s) 129, 515
HinfI GANTC 9 cut(s) 12, 119, 462, 577, 629, 645, 795, 869, 881
HpaII CCGG 3 cut(s) 64, 78, 335
HphI GGTGA 2 cut(s) 305, 787
Hpy166II GTNNAC 4 cut(s) 85, 181, 302, 684
Hpy188I TCNGA 2 cut(s) 43, 502
Hpy188III TCNNGA 8 cut(s) 221, 335, 425, 581, 607, 649, 770, 860
Hpy8I GTNNAC 4 cut(s) 85, 181, 302, 684
HpyAV CCTTC 2 cut(s) 271, 702
HpyCH4III ACNGT 4 cut(s) 55, 172, 656, 828
HpyCH4IV ACGT 1 cut(s) 36
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 87, 476
HpyF3I CTNAG 4 cut(s) 573, 696, 815, 906
HpySE526I ACGT 1 cut(s) 36
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 5 cut(s) 383, 400, 538, 662, 690
HspAI GCGC 1 cut(s) 239
Kpn2I TCCGGA 1 cut(s) 334
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Kzo9I GATC 2 cut(s) 107, 217
Lsp1109I GCAGC 5 cut(s) 61, 378, 406, 428, 862
LweI GCATC 3 cut(s) 99, 142, 182
MaeI CTAG 1 cut(s) 189
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 1 cut(s) 97
MalI GATC 2 cut(s) 109, 219
MbiI CCGCTC 1 cut(s) 213
MboI GATC 2 cut(s) 107, 217
MboII GAAGA 3 cut(s) 577, 712, 731
MfeI CAATTG 2 cut(s) 138, 288
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 2 cut(s) 183, 900
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 7 cut(s) 138, 200, 288, 339, 371, 438, 671
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 1 cut(s) 6
MnlI CCTC 6 cut(s) 109, 253, 342, 585, 766, 867
Mox20I TGGCCA 1 cut(s) 254
MroI TCCGGA 1 cut(s) 334
MroNI GCCGGC 1 cut(s) 77
MroXI GAANNNNTTC 1 cut(s) 461
MscI TGGCCA 1 cut(s) 254
MseI TTAA 3 cut(s) 134, 669, 732
MslI CAYNNNNRTG 1 cut(s) 411
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 2 cut(s) 419, 814
MspI CCGG 3 cut(s) 64, 78, 335
MspR9I CCNGG 2 cut(s) 64, 65
MunI CAATTG 2 cut(s) 138, 288
Mva1269I GAATGC 1 cut(s) 597
MwoI GCNNNNNNNGC 3 cut(s) 74, 87, 476
NaeI GCCGGC 1 cut(s) 79
NciI CCSGG 2 cut(s) 64, 65
NdeI CATATG 1 cut(s) 408
NdeII GATC 2 cut(s) 107, 217
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 5 cut(s) 383, 400, 538, 662, 690
NlaIV GGNNCC 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 97
NspI RCATGY 2 cut(s) 383, 538
PciI ACATGT 1 cut(s) 534
PctI GAATGC 1 cut(s) 597
PdiI GCCGGC 1 cut(s) 79
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 8 cut(s) 119, 462, 577, 629, 645, 795, 869, 881
PflMI CCANNNNNTGG 1 cut(s) 641
PkrI GCNGC 5 cut(s) 76, 393, 418, 421, 877
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
PscI ACATGT 1 cut(s) 534
PspFI CCCAGC 3 cut(s) 470, 662, 893
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 478
PstI CTGCAG 1 cut(s) 880
PsuI RGATCY 1 cut(s) 217
PvuII CAGCTG 2 cut(s) 419, 814
RsaI GTAC 2 cut(s) 169, 830
RsaNI GTAC 2 cut(s) 168, 829
RseI CAYNNNNRTG 1 cut(s) 411
SaqAI TTAA 3 cut(s) 134, 669, 732
SatI GCNGC 5 cut(s) 75, 392, 417, 420, 876
Sau3AI GATC 2 cut(s) 107, 217
Sau96I GGNCC 1 cut(s) 478
SchI GAGTC 1 cut(s) 6
ScrFI CCNGG 2 cut(s) 64, 65
SduI GDGCHC 2 cut(s) 183, 900
SfaNI GCATC 3 cut(s) 99, 142, 182
SfcI CTRYAG 1 cut(s) 876
SmaI CCCGGG 1 cut(s) 65
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 4 cut(s) 113, 423, 770, 858
SmoI CTYRAG 4 cut(s) 113, 423, 770, 858
Sse9I AATT 7 cut(s) 138, 200, 288, 339, 371, 438, 671
SsiI CCGC 3 cut(s) 88, 213, 739
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 62, 63
TaaI ACNGT 4 cut(s) 55, 172, 656, 828
TaiI ACGT 1 cut(s) 39
TaqI TCGA 1 cut(s) 606
TasI AATT 7 cut(s) 138, 200, 288, 339, 371, 438, 671
TatI WGTACW 1 cut(s) 167
TfiI GAWTC 8 cut(s) 119, 462, 577, 629, 645, 795, 869, 881
Tru1I TTAA 3 cut(s) 134, 669, 732
Tru9I TTAA 3 cut(s) 134, 669, 732
TscAI CASTG 5 cut(s) 35, 106, 472, 805, 831
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 5 cut(s) 74, 391, 416, 419, 875
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 4 cut(s) 450, 474, 732, 759
TspMI CCCGGG 1 cut(s) 63
TspRI CASTG 5 cut(s) 35, 106, 472, 805, 831
Van91I CCANNNNNTGG 1 cut(s) 641
VneI GTGCAC 1 cut(s) 179
XapI RAATTY 3 cut(s) 200, 339, 671
XceI RCATGY 2 cut(s) 383, 538
XcmI CCANNNNNNNNNTGG 1 cut(s) 488
XmaI CCCGGG 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.