MD12G1041900.v1.1

deoxyribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009395
Physical Location & Seq
Forward (+)
4640711 .. 4645036
4326 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1041900.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGCAACAATCCGCATGATAGACATAGCCGTCAACTTCACAGATGGAATGTTCAGAGGAGTATACAATGGAAAGCAGTGCCATGTCTCGGATATAGCCACGGTTTTGAGCAGGGCTTGGACTGCCGGCGTCGACCGCATTATCGTCACTGGTGGATCACTTGAGGAATCAAAGGAAGCTCTAACAATTGCGGAAACCGATGCAAGGCTTTTTTGTACGGTTGGTGTGCACCCCACTAGATGCAAGGAATTTGAAGAGAGTGGGGATCCAGATAAGCATTTTCAGGCGCTTCTGTCATTGGCCAAAGAGGGCATCGAGAAGGGAAAGGTGGTTGCAATTGGTGAGTGTGGACTGGACTATGACAGGCTTCAGTTTTGCCCAGCAGAGATCCAAAAAAAGTATTTTGAGAAGCAGTTTGAATTGGCACACACCACTAAGCTTCCCATGTTTCTGCATATGCGTGCAGCTGCTCCAGATTTCTGTGAAATTGTAGAGAGAAACAGGGGCAGATTCAGTGCTGGGGTCGCCCACTCATTTACTGGCAGTGCAGAAGATCGTGATAAACTTCTTTCCATCAACAACATGTATATAGGAGTAAATGGTTGCTCTCTGAAGACAACTGAGAATCTCGACGTTGTGAGGGACATTCCTATTGAGAGGATGATGATTGAGACAGACTCTCCTTATTGTGGAATCAAGAACACAAGTGCTGGGATTAAATTTGTGAAGTCCATTTGGCCTTCGAAGAAGAAAGAGAAGTATGATCAAGAGTGCATTGTTAAAGACCGCAATGAACCGTGTTTGGTTCGGCAAGTTCTTGAGGTGGTAGCTGGTTGTAAGGGTATCAATGACATAGGTCAGCTGAGCCGGACATTGTATCACAATACTTGCAGGGTTTTCTTTCCTCAGGATTTGGATTCTGCAGCAGATTCTTTACTTGATGGTCATGACCCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TatD_DNase

Protein Analysis

320

Amino Acids

35.58

Weight (kDa)

5.82

Isoelectric Point (pI)

29.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TatD_DNase PF01026 30 - 300 2.8e-51 TatD related DNase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 29
AccI GTMKAC 2 cut(s) 63, 132
AciI CCGC 4 cut(s) 13, 136, 191, 787
AclWI GGATC 4 cut(s) 163, 260, 273, 382
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 2 cut(s) 248, 719
AcuI CTGAAG 2 cut(s) 353, 632
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 1 cut(s) 217
AfiI CCNNNNNNNGG 3 cut(s) 88, 204, 689
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 2 cut(s) 254, 419
AluBI AGCT 5 cut(s) 179, 439, 467, 830, 862
AluI AGCT 5 cut(s) 179, 439, 467, 830, 862
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 2 cut(s) 91, 665
Alw44I GTGCAC 1 cut(s) 227
AlwI GGATC 4 cut(s) 163, 260, 273, 382
AoxI GGCC 2 cut(s) 300, 737
ApaLI GTGCAC 1 cut(s) 227
ApeKI GCWGC 3 cut(s) 464, 467, 923
ApoI RAATTY 2 cut(s) 248, 719
AspLEI GCGC 1 cut(s) 289
AsuHPI GGTGA 1 cut(s) 353
AsuII TTCGAA 1 cut(s) 743
AxyI CCTNAGG 1 cut(s) 906
BaeGI GKGCMC 1 cut(s) 231
BalI TGGCCA 1 cut(s) 302
BamHI GGATCC 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 3 cut(s) 454, 476, 935
BccI CCATC 3 cut(s) 38, 581, 935
BceAI ACGGC 1 cut(s) 14
BclI TGATCA 1 cut(s) 763
BcoDI GTCTC 2 cut(s) 91, 665
BfaI CTAG 1 cut(s) 237
BfmI CTRYAG 1 cut(s) 921
BfoI RGCGCY 1 cut(s) 290
BisI GCNGC 3 cut(s) 465, 468, 924
BlpI GCTNAGC 1 cut(s) 863
BlsI GCNGC 3 cut(s) 466, 469, 925
BmiI GGNNCC 1 cut(s) 267
BmsI GCATC 3 cut(s) 190, 230, 321
BoxI GACNNNNGTC 1 cut(s) 855
BpiI GAAGAC 1 cut(s) 620
BplI GAGNNNNNCTC 2 cut(s) 662, 694
BpmI CTGGAG 1 cut(s) 456
Bpu1102I GCTNAGC 1 cut(s) 863
Bpu14I TTCGAA 1 cut(s) 743
BpuEI CTTGAG 2 cut(s) 182, 839
BsaHI GRCGYC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 204, 689
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 3 cut(s) 154, 357, 544
Bse21I CCTNAGG 1 cut(s) 906
Bse3DI GCAATG 1 cut(s) 796
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 1 cut(s) 666
BseLI CCNNNNNNNGG 3 cut(s) 88, 204, 689
BseMI GCAATG 1 cut(s) 796
BseMII CTCAG 3 cut(s) 612, 854, 920
BseNI ACTGG 3 cut(s) 154, 357, 544
BseRI GAGGAG 1 cut(s) 72
BseSI GKGCMC 1 cut(s) 231
BseXI GCAGC 3 cut(s) 454, 476, 935
BseYI CCCAGC 3 cut(s) 379, 518, 710
BsgI GTGCAG 2 cut(s) 483, 567
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 2 cut(s) 302, 739
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 2 cut(s) 126, 868
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 3 cut(s) 88, 204, 689
BsmAI GTCTC 2 cut(s) 91, 665
BsmFI GGGAC 1 cut(s) 656
BsnI GGCC 2 cut(s) 302, 739
Bsp119I TTCGAA 1 cut(s) 743
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 5 cut(s) 155, 265, 387, 553, 763
Bsp1720I GCTNAGC 1 cut(s) 863
BspACI CCGC 4 cut(s) 13, 136, 191, 787
BspANI GGCC 2 cut(s) 302, 739
BspCNI CTCAG 3 cut(s) 613, 855, 919
BspHI TCATGA 1 cut(s) 946
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 925
BspPI GGATC 4 cut(s) 163, 260, 273, 382
BspT104I TTCGAA 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 796
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 3 cut(s) 154, 357, 544
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 5 cut(s) 155, 265, 387, 553, 763
BssNAI GTATAC 1 cut(s) 64
BssNI GRCGYC 1 cut(s) 129
Bst1107I GTATAC 1 cut(s) 64
Bst4CI ACNGT 3 cut(s) 103, 220, 798
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 1 cut(s) 129
BstBI TTCGAA 1 cut(s) 743
BstC8I GCNNGC 2 cut(s) 127, 462
BstDEI CTNAG 5 cut(s) 435, 621, 863, 906, 954
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 1 cut(s) 666
BstH2I RGCGCY 1 cut(s) 290
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 5 cut(s) 158, 268, 390, 556, 766
BstMAI GTCTC 2 cut(s) 91, 665
BstMBI GATC 5 cut(s) 155, 265, 387, 553, 763
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 3 cut(s) 122, 135, 524
BstNSI RCATGY 1 cut(s) 586
BstPAI GACNNNNGTC 1 cut(s) 855
BstSFI CTRYAG 1 cut(s) 921
BstSLI GKGCMC 1 cut(s) 231
BstV1I GCAGC 3 cut(s) 454, 476, 935
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 2 cut(s) 265, 387
BstYI RGATCY 2 cut(s) 265, 387
BstZ17I GTATAC 1 cut(s) 64
Bsu36I CCTNAGG 1 cut(s) 906
BsuRI GGCC 2 cut(s) 302, 739
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 1 cut(s) 666
BtsI GCAGTG 2 cut(s) 83, 550
BtsIMutI CAGTG 4 cut(s) 83, 147, 520, 550
Cac8I GCNNGC 2 cut(s) 127, 462
CciI TCATGA 1 cut(s) 946
CfoI GCGC 1 cut(s) 289
Cfr10I RCCGGY 1 cut(s) 125
CseI GACGC 1 cut(s) 118
Csp6I GTAC 1 cut(s) 216
CviAII CATG 5 cut(s) 16, 83, 445, 583, 947
CviQI GTAC 1 cut(s) 216
DdeI CTNAG 5 cut(s) 435, 621, 863, 906, 954
DpnI GATC 5 cut(s) 157, 267, 389, 555, 765
DpnII GATC 5 cut(s) 155, 265, 387, 553, 763
DrdI GACNNNNNNGTC 1 cut(s) 29
DseDI GACNNNNNNGTC 1 cut(s) 29
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco57I CTGAAG 2 cut(s) 353, 632
Eco81I CCTNAGG 1 cut(s) 906
FaeI CATG 5 cut(s) 19, 86, 448, 586, 950
FaqI GGGAC 1 cut(s) 656
FatI CATG 5 cut(s) 15, 82, 444, 582, 946
FauNDI CATATG 1 cut(s) 456
FbaI TGATCA 1 cut(s) 763
FblI GTMKAC 2 cut(s) 63, 132
Fnu4HI GCNGC 3 cut(s) 465, 468, 924
FokI GGATG 1 cut(s) 673
Fsp4HI GCNGC 3 cut(s) 465, 468, 924
FspBI CTAG 1 cut(s) 237
GlaI GCGC 1 cut(s) 288
GluI GCNGC 3 cut(s) 465, 468, 924
GsaI CCCAGC 3 cut(s) 383, 522, 714
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 290
HaeIII GGCC 2 cut(s) 302, 739
HapII CCGG 2 cut(s) 126, 868
HgaI GACGC 1 cut(s) 118
HhaI GCGC 1 cut(s) 289
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 5 cut(s) 19, 86, 448, 586, 950
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HincII GTYRAC 2 cut(s) 34, 133
HindII GTYRAC 2 cut(s) 34, 133
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 7 cut(s) 167, 510, 625, 677, 693, 917, 929
HpaII CCGG 2 cut(s) 126, 868
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 5 cut(s) 34, 64, 133, 229, 350
Hpy188I TCNGA 3 cut(s) 56, 91, 612
Hpy8I GTNNAC 5 cut(s) 34, 64, 133, 229, 350
Hpy99I CGWCG 2 cut(s) 134, 635
HpyAV CCTTC 2 cut(s) 313, 750
HpyCH4III ACNGT 3 cut(s) 103, 220, 798
HpyCH4IV ACGT 1 cut(s) 633
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 135, 524
HpyF3I CTNAG 5 cut(s) 435, 621, 863, 906, 954
HpySE526I ACGT 1 cut(s) 633
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 5 cut(s) 19, 86, 448, 586, 950
HspAI GCGC 1 cut(s) 287
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 763
Kzo9I GATC 5 cut(s) 155, 265, 387, 553, 763
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 3 cut(s) 454, 476, 935
LweI GCATC 3 cut(s) 190, 230, 321
MaeI CTAG 1 cut(s) 237
MaeII ACGT 1 cut(s) 633
MaeIII GTNAC 1 cut(s) 145
MalI GATC 5 cut(s) 157, 267, 389, 555, 765
MboI GATC 5 cut(s) 155, 265, 387, 553, 763
MboII GAAGA 5 cut(s) 266, 563, 625, 757, 760
MfeI CAATTG 2 cut(s) 186, 336
MflI RGATCY 2 cut(s) 265, 387
MhlI GDGCHC 1 cut(s) 231
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 6 cut(s) 186, 248, 336, 419, 486, 719
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 1 cut(s) 671
MnlI CCTC 7 cut(s) 50, 157, 301, 633, 651, 814, 915
Mox20I TGGCCA 1 cut(s) 302
MroNI GCCGGC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 302
MseI TTAA 2 cut(s) 717, 780
MslI CAYNNNNRTG 1 cut(s) 459
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 2 cut(s) 467, 862
MspI CCGG 2 cut(s) 126, 868
MunI CAATTG 2 cut(s) 186, 336
MwoI GCNNNNNNNGC 3 cut(s) 122, 135, 524
NaeI GCCGGC 1 cut(s) 127
NdeI CATATG 1 cut(s) 456
NdeII GATC 5 cut(s) 155, 265, 387, 553, 763
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 5 cut(s) 19, 86, 448, 586, 950
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 145
NspI RCATGY 1 cut(s) 586
NspV TTCGAA 1 cut(s) 743
PagI TCATGA 1 cut(s) 946
PciI ACATGT 1 cut(s) 582
PdiI GCCGGC 1 cut(s) 127
PfeI GAWTC 6 cut(s) 167, 510, 625, 693, 917, 929
PkrI GCNGC 3 cut(s) 466, 469, 925
PleI GAGTC 1 cut(s) 671
PpsI GAGTC 1 cut(s) 671
PscI ACATGT 1 cut(s) 582
PshAI GACNNNNGTC 1 cut(s) 855
PspFI CCCAGC 3 cut(s) 379, 518, 710
PspN4I GGNNCC 1 cut(s) 267
PstI CTGCAG 1 cut(s) 925
PsuI RGATCY 2 cut(s) 265, 387
PvuII CAGCTG 2 cut(s) 467, 862
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
RseI CAYNNNNRTG 1 cut(s) 459
SalI GTCGAC 1 cut(s) 131
SaqAI TTAA 2 cut(s) 717, 780
SatI GCNGC 3 cut(s) 465, 468, 924
Sau3AI GATC 5 cut(s) 155, 265, 387, 553, 763
SchI GAGTC 1 cut(s) 671
SduI GDGCHC 1 cut(s) 231
SetI ASST 9 cut(s) 181, 330, 441, 469, 636, 825, 832, 859, 864
SfaNI GCATC 3 cut(s) 190, 230, 321
SfcI CTRYAG 1 cut(s) 921
SfuI TTCGAA 1 cut(s) 743
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 2 cut(s) 161, 818
SmoI CTYRAG 2 cut(s) 161, 818
Sse9I AATT 6 cut(s) 186, 248, 336, 419, 486, 719
SsiI CCGC 4 cut(s) 13, 136, 191, 787
SspMI CTAG 1 cut(s) 237
TaaI ACNGT 3 cut(s) 103, 220, 798
TaiI ACGT 1 cut(s) 636
TaqI TCGA 4 cut(s) 132, 315, 630, 743
TasI AATT 6 cut(s) 186, 248, 336, 419, 486, 719
TfiI GAWTC 6 cut(s) 167, 510, 625, 693, 917, 929
Tru1I TTAA 2 cut(s) 717, 780
Tru9I TTAA 2 cut(s) 717, 780
TscAI CASTG 4 cut(s) 83, 154, 520, 550
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 3 cut(s) 464, 467, 923
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 1 cut(s) 807
TspRI CASTG 4 cut(s) 83, 154, 520, 550
VneI GTGCAC 1 cut(s) 227
XapI RAATTY 2 cut(s) 248, 719
XceI RCATGY 1 cut(s) 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XmiI GTMKAC 2 cut(s) 63, 132
XspI CTAG 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.