Rroxscaffold_6G00405370

deoxyribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009395
Physical Location & Seq
Reverse (-)
27761614 .. 27766208
4595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00405370.1

Sequence Viewer

Length: 1278 bp
ATGTCGAAGAAGGAGGGCGAAACCTCCGCGTCCGGACTTGATTCCCGCAAAAGCGGAAACCAAAAGCTACAAATTGTAGCTTCTGATTTCTGCGGTTTAGCGAAGCGCTTCGGCCAAAAGCTACTCTCGAATGGTTTACCAAACAGCCCAGATAACTTTGAAAGCGCTTTTGGTCCTAGAAAGCCAGCAGGTCTTTCTTCCTCTCTTCGCTATCAGTTTGGCCATGGCGACGGTCCGCATGATAGGCAAGTAACTCTTCCACAACCTCTTTCTACAATTATTCTCACCTCCTCAATACTAATTCTCTCATTCTCTGCGATTCCGGTGACTTCAAATACAGATATAGCCGTTAACTTCACAGATGGAATGTTCAAAGGACTCTACAATGGGAAGCAGTGCCACGTCTCCGATATACCCACCGTTTTGACCCGGGCTTGGGCTGCCGGCGTCCACCGCATCATCGTCACTGGTGGATCACTTGAGGAATCAAAGGAAGCTTTAACAATTGCACAAACTGATGCAAGGCTTTTTTGTACTGTGGGTGTGCACCCAACTAGATGCAAGGAATTTGATGACAGCGGGGATCCAGAAAAGCATTTTCAGGCGCTTTTGTCATTGGCCAGAGAGGGGATAGAGAAAGGGAAGGTGGTTGCAATTGGTGAGTGTGGACTGGACTATGACAGGCTTCAGTTTTGCTCTCCGGAAATTCAAAAGAAGTATTTTGAGAAGCAGTTTGAATTGGCACATGCCACTAAGCTGCCCATGTTTCTGCATATGCGTGCAGCTGCTCAAGATTTCTGTGAAATTGTAGAAAGGAATAGGAACAGATTCACTGCTGGGGTGGCCCATTCATTTACTGGTAGTTCAGACGATTGTGATAAGCTTCTTTCCATCAGCAACATGTATATAGGTGTGAATGGTTGCTCTTTGAAGACAACTGAGAATCTTGATGTTGTGAGGGGCATTCCTGTTGACAGAATGATGATTGAAACAGATTCTCCATATTGTGGAATCAAGAACAGTCATGCTGGGATTAAATTTGTGAAGTCCACATGGCCTTCTAAGAAGAAAGAGAAGTATGATCAAGAGTGCATTGTTAAAGACCGCAATGAACCTTGTTTGGTTCTGCAAGTTCTTGAGGTGATAGCTGGTTGTAAAGGAATCAGTGATGAAGGTCAGCTGAGCAGGACACTGTACCAGAACACTTGCAGGGTTTTCTTTCCTCAAGACTTGGATTCTGCTGCAGATTCTTTACTTGCTGGGCACGATCCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TatD_DNase

Protein Analysis

425

Amino Acids

46.64

Weight (kDa)

6.6

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TatD_DNase PF01026 136 - 406 3.1e-51 TatD related DNase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 179
AccB7I CCANNNNNTGG 1 cut(s) 1007
AccII CGCG 1 cut(s) 29
AccIII TCCGGA 2 cut(s) 32, 700
AciI CCGC 8 cut(s) 27, 46, 54, 93, 236, 454, 579, 1105
AclWI GGATC 4 cut(s) 481, 578, 591, 1262
AcoI YGGCCR 3 cut(s) 112, 220, 618
AcsI RAATTY 3 cut(s) 566, 705, 1037
AcuI CTGAAG 1 cut(s) 671
AcyI GRCGYC 1 cut(s) 447
AfaI GTAC 2 cut(s) 535, 1196
AfeI AGCGCT 2 cut(s) 107, 166
AfiI CCNNNNNNNGG 4 cut(s) 435, 436, 627, 1007
AflIII ACRYGT 1 cut(s) 900
AgsI TTSAA 7 cut(s) 161, 333, 373, 710, 737, 931, 989
AjiI CACGTC 1 cut(s) 403
AjuI GAANNNNNNNTTGG 2 cut(s) 153, 185
AluBI AGCT 9 cut(s) 67, 80, 121, 497, 757, 785, 883, 1148, 1180
AluI AGCT 9 cut(s) 67, 80, 121, 497, 757, 785, 883, 1148, 1180
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 1 cut(s) 409
Alw44I GTGCAC 1 cut(s) 545
AlwI GGATC 4 cut(s) 481, 578, 591, 1262
Ama87I CYCGRG 1 cut(s) 429
Aor13HI TCCGGA 2 cut(s) 32, 700
Aor51HI AGCGCT 2 cut(s) 107, 166
AoxI GGCC 5 cut(s) 112, 220, 618, 843, 1055
ApaLI GTGCAC 1 cut(s) 545
ApeKI GCWGC 5 cut(s) 440, 757, 782, 785, 1241
ApoI RAATTY 3 cut(s) 566, 705, 1037
Asp700I GAANNNNTTC 2 cut(s) 107, 827
AspLEI GCGC 3 cut(s) 108, 167, 607
AspS9I GGNCC 3 cut(s) 173, 233, 844
AsuC2I CCSGG 2 cut(s) 430, 431
AsuHPI GGTGA 4 cut(s) 277, 337, 671, 1153
AvaI CYCGRG 1 cut(s) 429
AvaII GGWCC 2 cut(s) 173, 233
BaeGI GKGCMC 2 cut(s) 549, 1266
BalI TGGCCA 2 cut(s) 222, 620
BamHI GGATCC 1 cut(s) 583
BbsI GAAGAC 1 cut(s) 938
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 5 cut(s) 427, 744, 772, 794, 1228
BccI CCATC 2 cut(s) 356, 899
BceAI ACGGC 1 cut(s) 332
BclI TGATCA 1 cut(s) 1081
BcnI CCSGG 2 cut(s) 430, 431
BcoDI GTCTC 1 cut(s) 409
BfaI CTAG 2 cut(s) 177, 555
BfmI CTRYAG 1 cut(s) 1242
BfoI RGCGCY 3 cut(s) 109, 168, 608
BfuAI ACCTGC 1 cut(s) 179
BisI GCNGC 5 cut(s) 441, 758, 783, 786, 1242
BlpI GCTNAGC 1 cut(s) 1181
BlsI GCNGC 5 cut(s) 442, 759, 784, 787, 1243
Bme1390I CCNGG 2 cut(s) 430, 431
Bme18I GGWCC 2 cut(s) 173, 233
BmeT110I CYCGRG 1 cut(s) 429
BmgBI CACGTC 1 cut(s) 403
BmgT120I GGNCC 3 cut(s) 173, 233, 844
BmiI GGNNCC 1 cut(s) 585
BmrFI CCNGG 2 cut(s) 430, 431
BmsI GCATC 3 cut(s) 465, 508, 548
BpiI GAAGAC 1 cut(s) 938
Bpu1102I GCTNAGC 1 cut(s) 1181
BpuEI CTTGAG 4 cut(s) 500, 774, 1157, 1209
BpuMI CCSGG 2 cut(s) 430, 431
BsaHI GRCGYC 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 223, 429
BsaWI WCCGGW 3 cut(s) 32, 322, 700
BsaXI ACNNNNNCTCC 2 cut(s) 982, 1012
Bsc4I CCNNNNNNNGG 4 cut(s) 435, 436, 627, 1007
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 3 cut(s) 472, 675, 862
Bse3DI GCAATG 1 cut(s) 1114
BseAI TCCGGA 2 cut(s) 32, 700
BseDI CCNNGG 2 cut(s) 223, 429
BseLI CCNNNNNNNGG 4 cut(s) 435, 436, 627, 1007
BseMI GCAATG 1 cut(s) 1114
BseMII CTCAG 2 cut(s) 930, 1172
BseNI ACTGG 3 cut(s) 472, 675, 862
BseRI GAGGAG 1 cut(s) 280
BseSI GKGCMC 2 cut(s) 549, 1266
BseXI GCAGC 5 cut(s) 427, 744, 772, 794, 1228
BseYI CCCAGC 3 cut(s) 836, 1028, 1259
BsgI GTGCAG 1 cut(s) 801
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 5 cut(s) 114, 222, 620, 845, 1057
BsiHKAI GWGCWC 1 cut(s) 549
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 5 cut(s) 33, 323, 430, 444, 701
BslI CCNNNNNNNGG 4 cut(s) 435, 436, 627, 1007
BsmAI GTCTC 1 cut(s) 409
BsmBI CGTCTC 1 cut(s) 409
BsmI GAATGC 1 cut(s) 963
BsnI GGCC 5 cut(s) 114, 222, 620, 845, 1057
BsoBI CYCGRG 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 549, 1266
Bsp13I TCCGGA 2 cut(s) 32, 700
Bsp143I GATC 4 cut(s) 473, 583, 1081, 1267
Bsp1720I GCTNAGC 1 cut(s) 1181
Bsp19I CCATGG 1 cut(s) 223
BspACI CCGC 8 cut(s) 27, 46, 54, 93, 236, 454, 579, 1105
BspANI GGCC 5 cut(s) 114, 222, 620, 845, 1057
BspCNI CTCAG 2 cut(s) 931, 1173
BspEI TCCGGA 2 cut(s) 32, 700
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 1 cut(s) 585
BspMAI CTGCAG 1 cut(s) 1246
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 4 cut(s) 481, 578, 591, 1262
BsrDI GCAATG 1 cut(s) 1114
BsrFI RCCGGY 1 cut(s) 443
BsrI ACTGG 3 cut(s) 472, 675, 862
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 2 cut(s) 223, 429
BssMI GATC 4 cut(s) 473, 583, 1081, 1267
BssNI GRCGYC 1 cut(s) 447
BssT1I CCWWGG 1 cut(s) 223
Bst4CI ACNGT 5 cut(s) 233, 421, 538, 1022, 1194
Bst6I CTCTTC 2 cut(s) 210, 261
BstACI GRCGYC 1 cut(s) 447
BstC8I GCNNGC 3 cut(s) 186, 445, 780
BstDEI CTNAG 5 cut(s) 753, 939, 1062, 1181, 1272
BstDSI CCRYGG 1 cut(s) 223
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 3 cut(s) 109, 168, 608
BstHHI GCGC 3 cut(s) 108, 167, 607
BstKTI GATC 4 cut(s) 476, 586, 1084, 1270
BstMAI GTCTC 1 cut(s) 409
BstMBI GATC 4 cut(s) 473, 583, 1081, 1267
BstMWI GCNNNNNNNGC 4 cut(s) 244, 440, 453, 842
BstNSI RCATGY 2 cut(s) 749, 904
BstSCI CCNGG 2 cut(s) 428, 429
BstSFI CTRYAG 1 cut(s) 1242
BstSLI GKGCMC 2 cut(s) 549, 1266
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 5 cut(s) 427, 744, 772, 794, 1228
BstV2I GAAGAC 1 cut(s) 938
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BsuRI GGCC 5 cut(s) 114, 222, 620, 845, 1057
BtgI CCRYGG 1 cut(s) 223
BtrI CACGTC 1 cut(s) 403
BtsI GCAGTG 2 cut(s) 401, 831
BtsIMutI CAGTG 5 cut(s) 401, 465, 831, 1171, 1190
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 3 cut(s) 186, 445, 780
CfoI GCGC 3 cut(s) 108, 167, 607
Cfr10I RCCGGY 1 cut(s) 443
Cfr13I GGNCC 3 cut(s) 173, 233, 844
Cfr9I CCCGGG 1 cut(s) 429
CpoI CGGWCCG 1 cut(s) 233
CseI GACGC 2 cut(s) 18, 436
Csp6I GTAC 2 cut(s) 534, 1195
CspI CGGWCCG 1 cut(s) 233
CviAII CATG 7 cut(s) 224, 239, 746, 763, 901, 1025, 1053
CviQI GTAC 2 cut(s) 534, 1195
DdeI CTNAG 5 cut(s) 753, 939, 1062, 1181, 1272
DpnI GATC 4 cut(s) 475, 585, 1083, 1269
DpnII GATC 4 cut(s) 473, 583, 1081, 1267
EaeI YGGCCR 3 cut(s) 112, 220, 618
Eam1104I CTCTTC 2 cut(s) 210, 261
EarI CTCTTC 2 cut(s) 210, 261
Eco130I CCWWGG 1 cut(s) 223
Eco47I GGWCC 2 cut(s) 173, 233
Eco47III AGCGCT 2 cut(s) 107, 166
Eco57I CTGAAG 1 cut(s) 671
Eco88I CYCGRG 1 cut(s) 429
EcoT14I CCWWGG 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 223
Esp3I CGTCTC 1 cut(s) 409
FaeI CATG 7 cut(s) 227, 242, 749, 766, 904, 1028, 1056
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FatI CATG 7 cut(s) 223, 238, 745, 762, 900, 1024, 1052
FauI CCCGC 2 cut(s) 53, 572
FauNDI CATATG 1 cut(s) 774
FbaI TGATCA 1 cut(s) 1081
Fnu4HI GCNGC 5 cut(s) 441, 758, 783, 786, 1242
Fsp4HI GCNGC 5 cut(s) 441, 758, 783, 786, 1242
FspBI CTAG 2 cut(s) 177, 555
GlaI GCGC 3 cut(s) 107, 166, 606
GluI GCNGC 5 cut(s) 441, 758, 783, 786, 1242
GsaI CCCAGC 3 cut(s) 840, 1032, 1263
HaeII RGCGCY 3 cut(s) 109, 168, 608
HaeIII GGCC 5 cut(s) 114, 222, 620, 845, 1057
HapII CCGG 5 cut(s) 33, 323, 430, 444, 701
HgaI GACGC 2 cut(s) 18, 436
HhaI GCGC 3 cut(s) 108, 167, 607
Hin1I GRCGYC 1 cut(s) 447
Hin1II CATG 7 cut(s) 227, 242, 749, 766, 904, 1028, 1056
Hin6I GCGC 3 cut(s) 106, 165, 605
HinP1I GCGC 3 cut(s) 106, 165, 605
HincII GTYRAC 2 cut(s) 352, 973
HindII GTYRAC 2 cut(s) 352, 973
HindIII AAGCTT 2 cut(s) 495, 881
HpaI GTTAAC 1 cut(s) 352
HpaII CCGG 5 cut(s) 33, 323, 430, 444, 701
HphI GGTGA 4 cut(s) 277, 337, 671, 1153
Hpy166II GTNNAC 7 cut(s) 137, 352, 451, 547, 668, 973, 1050
Hpy188I TCNGA 3 cut(s) 85, 409, 868
Hpy8I GTNNAC 7 cut(s) 137, 352, 451, 547, 668, 973, 1050
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 4 cut(s) 4, 637, 1068, 1166
HpyCH4III ACNGT 5 cut(s) 233, 421, 538, 1022, 1194
HpyCH4IV ACGT 1 cut(s) 402
HpyF10VI GCNNNNNNNGC 4 cut(s) 244, 440, 453, 842
HpyF3I CTNAG 5 cut(s) 753, 939, 1062, 1181, 1272
HpySE526I ACGT 1 cut(s) 402
Hsp92I GRCGYC 1 cut(s) 447
Hsp92II CATG 7 cut(s) 227, 242, 749, 766, 904, 1028, 1056
HspAI GCGC 3 cut(s) 106, 165, 605
Kpn2I TCCGGA 2 cut(s) 32, 700
KroI GCCGGC 1 cut(s) 443
KroNI GCCGGC 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 1081
KspAI GTTAAC 1 cut(s) 352
Kzo9I GATC 4 cut(s) 473, 583, 1081, 1267
Lsp1109I GCAGC 5 cut(s) 427, 744, 772, 794, 1228
LweI GCATC 3 cut(s) 465, 508, 548
MaeI CTAG 2 cut(s) 177, 555
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 3 cut(s) 250, 325, 463
MalI GATC 4 cut(s) 475, 585, 1083, 1269
MboI GATC 4 cut(s) 473, 583, 1081, 1267
MboII GAAGA 6 cut(s) 19, 189, 197, 248, 943, 1078
MfeI CAATTG 2 cut(s) 504, 654
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 2 cut(s) 549, 1266
MlsI TGGCCA 2 cut(s) 222, 620
MluNI TGGCCA 2 cut(s) 222, 620
MlyI GAGTC 1 cut(s) 372
Mox20I TGGCCA 2 cut(s) 222, 620
MroI TCCGGA 2 cut(s) 32, 700
MroNI GCCGGC 1 cut(s) 443
MroXI GAANNNNTTC 2 cut(s) 107, 827
MscI TGGCCA 2 cut(s) 222, 620
MseI TTAA 4 cut(s) 351, 500, 1035, 1098
MslI CAYNNNNRTG 1 cut(s) 777
Msp20I TGGCCA 2 cut(s) 222, 620
MspA1I CMGCKG 3 cut(s) 579, 785, 1180
MspI CCGG 5 cut(s) 33, 323, 430, 444, 701
MspR9I CCNGG 2 cut(s) 430, 431
MunI CAATTG 2 cut(s) 504, 654
Mva1269I GAATGC 1 cut(s) 963
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 4 cut(s) 244, 440, 453, 842
NaeI GCCGGC 1 cut(s) 445
NciI CCSGG 2 cut(s) 430, 431
NcoI CCATGG 1 cut(s) 223
NdeI CATATG 1 cut(s) 774
NdeII GATC 4 cut(s) 473, 583, 1081, 1267
NgoMIV GCCGGC 1 cut(s) 443
NlaIII CATG 7 cut(s) 227, 242, 749, 766, 904, 1028, 1056
NlaIV GGNNCC 1 cut(s) 585
NmuCI GTSAC 2 cut(s) 325, 463
NspI RCATGY 2 cut(s) 749, 904
PciI ACATGT 1 cut(s) 900
PctI GAATGC 1 cut(s) 963
PdiI GCCGGC 1 cut(s) 445
PdmI GAANNNNTTC 2 cut(s) 107, 827
PflMI CCANNNNNTGG 1 cut(s) 1007
PkrI GCNGC 5 cut(s) 442, 759, 784, 787, 1243
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
PscI ACATGT 1 cut(s) 900
PspFI CCCAGC 3 cut(s) 836, 1028, 1259
PspN4I GGNNCC 1 cut(s) 585
PspPI GGNCC 3 cut(s) 173, 233, 844
PstI CTGCAG 1 cut(s) 1246
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 2 cut(s) 785, 1180
RsaI GTAC 2 cut(s) 535, 1196
RsaNI GTAC 2 cut(s) 534, 1195
RseI CAYNNNNRTG 1 cut(s) 777
Rsr2I CGGWCCG 1 cut(s) 233
RsrII CGGWCCG 1 cut(s) 233
SaqAI TTAA 4 cut(s) 351, 500, 1035, 1098
SatI GCNGC 5 cut(s) 441, 758, 783, 786, 1242
Sau3AI GATC 4 cut(s) 473, 583, 1081, 1267
Sau96I GGNCC 3 cut(s) 173, 233, 844
SchI GAGTC 1 cut(s) 372
ScrFI CCNGG 2 cut(s) 430, 431
SduI GDGCHC 2 cut(s) 549, 1266
SfaNI GCATC 3 cut(s) 465, 508, 548
SfcI CTRYAG 1 cut(s) 1242
SinI GGWCC 2 cut(s) 173, 233
SmaI CCCGGG 1 cut(s) 431
SmiMI CAYNNNNRTG 1 cut(s) 777
SmlI CTYRAG 4 cut(s) 479, 789, 1136, 1224
SmoI CTYRAG 4 cut(s) 479, 789, 1136, 1224
SsiI CCGC 8 cut(s) 27, 46, 54, 93, 236, 454, 579, 1105
SspMI CTAG 2 cut(s) 177, 555
StyD4I CCNGG 2 cut(s) 428, 429
StyI CCWWGG 1 cut(s) 223
TaaI ACNGT 5 cut(s) 233, 421, 538, 1022, 1194
TaiI ACGT 1 cut(s) 405
TaqI TCGA 2 cut(s) 5, 128
TatI WGTACW 1 cut(s) 533
Tru1I TTAA 4 cut(s) 351, 500, 1035, 1098
Tru9I TTAA 4 cut(s) 351, 500, 1035, 1098
TscAI CASTG 5 cut(s) 401, 472, 838, 1171, 1197
TseFI GTSAC 2 cut(s) 325, 463
TseI GCWGC 5 cut(s) 440, 757, 782, 785, 1241
Tsp45I GTSAC 2 cut(s) 325, 463
TspDTI ATGAA 3 cut(s) 840, 1125, 1185
TspMI CCCGGG 1 cut(s) 429
TspRI CASTG 5 cut(s) 401, 472, 838, 1171, 1197
Van91I CCANNNNNTGG 1 cut(s) 1007
VneI GTGCAC 1 cut(s) 545
VpaK11BI GGWCC 2 cut(s) 173, 233
XapI RAATTY 3 cut(s) 566, 705, 1037
XceI RCATGY 2 cut(s) 749, 904
XcmI CCANNNNNNNNNTGG 1 cut(s) 854
XmaI CCCGGG 1 cut(s) 429
XmnI GAANNNNTTC 2 cut(s) 107, 827
XspI CTAG 2 cut(s) 177, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.