FvH4_6g21140

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012445
Physical Location & Seq
Forward (+)
14723052 .. 14727518
4467 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21140.t2

Sequence Viewer

Length: 1230 bp
ATGGCGGGCGGTAAACAAAGCGCGGCGGGGGTTCCTCTGCTGGAAAAGGCCGTGGGGGCTTCGAGGGGTCAGACGCTCGGAAACATAATCGTCTCGGTTGTTGGGACCGGAGTTCTTGGCCTTCCGTATGCTATGAGAATCGCTGGTTGGCTTGCTGGCTCGCTTGGCGTCATTGTCACTGGGATCTCCACCTACTACTGCATGCTCCTCCTTGTGCAATGCAGGGAGAAATATGCATCAGAAGAAGATTCAGTGGTTAAAAAGTCTTATGGTGATTTGGGGTATGAATGTATGGGAAGGAAAGGTCGTATTCTGACCGAATCCTTGATTTTGATTGCTCAGTGCGGGGGCTCTGTGGCTTATCTTGTGTTTGTTGGCCAAAATGTTTCATCTATGTTCAATGGCCATGGAATCTCGGTTAATTCTTGCATATTTTTGCTAGTGCCGGTGGAAATTGGGTTATCTTGGATAGGTAGTCTATCTGCTTTAGCTCCCTTCAGTATCTTTGCTGCCATCTGCAATTTGTTGGCTATGGCAATTGTGGTAAAGGAAGACATACAGCAAGCTGTAGAGGGTGAGTTTTCTTTTAGGGATAGGACGGCGATCACATCAAATATAGGAGGATTGCCATTCGCAGGAGGCGTTGCAGTGTTTTGTTTTGAGGGGTTCGGGATGACATTGGCTTTGGAAGCCTCTATGAAAGATAAAACCCAGTTCCCAAAATTGCTTGCTCAGGCTTTCACAGGGATAAGCATTGTCTACGCTTTGTTTGGATTCTTTGGATACATGGCTTACGGTGATCAAACAAGAGAGATTATCTCTCTCAATCTCCCTCAAAATTGGTCTTCCATAGCCGTTCAGATTGGCTTGTGCTTGGGTTTAGTATTTACATTCCCGATCATGCTCCACCCAATAAATGAGATCATAGAGGGAAACATAACAAAAGATTCATCTACAAGACTGGGAAAATTGGGAATATACATGAGTCGAGCAATGGTGGTGATGGTGTTGGCCATCTTCGCCTCATGTGTGCCCGGATTTGCAGTGTTTGCTTCACTTGTGGGAAGTACAGTATGTGCACTGATATCATTTGTATTGCCAGCCACATTTCATTTAAAATTATTTGGTTCTTCCCTACAGTTCTGGCAGAAAGCCTTGGATTTCTTCATTTTACTGTGTGGATTACTTTTTGCGGTATATGGTACTTACAATGCCATTGTCGGTGTATGA

Protein Analysis

410

Amino Acids

43.54

Weight (kDa)

7.45

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 21 - 406 3.7e-94 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11900 AT3G11900
fragaria_vesca FvH4_6g21140 FvH4_6g21140 FvH4_6g21140
malus_domestica MD12G1047000.v1.1
prunus_persica Prupe.7G088200_v2.0.a1 Prupe.7G088200_v2.0.a1
pyrus_communis pycom12g04050
rosa_chinensis RchiOBHm_Chr3g0477611
rosa_laevigata RLG00000023706
rosa_multiflora Rmu_sc0000775.1_g000010
rosa_roxburghii Rroxscaffold_6G00403830
rosa_rugosa Rorug03G0162300
rosa_samantha Rh3AG212300 Rh3BG246000 Rh3CG239600 Rh3DG239100
rosa_wichuraiana Rw3G019220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 759
AccII CGCG 1 cut(s) 23
AciI CCGC 6 cut(s) 5, 9, 23, 26, 345, 1193
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 3 cut(s) 376, 403, 1011
AcuI CTGAAG 1 cut(s) 481
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 2 cut(s) 1069, 1204
AfiI CCNNNNNNNGG 1 cut(s) 635
AgsI TTSAA 1 cut(s) 400
AjuI GAANNNNNNNTTGG 2 cut(s) 130, 162
AluBI AGCT 2 cut(s) 491, 566
AluI AGCT 2 cut(s) 491, 566
Alw21I GWGCWC 1 cut(s) 1081
Alw26I GTCTC 1 cut(s) 97
Alw44I GTGCAC 1 cut(s) 1077
AlwI GGATC 1 cut(s) 191
AoxI GGCC 5 cut(s) 48, 118, 376, 403, 1011
ApaLI GTGCAC 1 cut(s) 1077
ApeKI GCWGC 1 cut(s) 509
ArsI GACNNNNNNTTYG 2 cut(s) 667, 699
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 1 cut(s) 105
AsuC2I CCSGG 1 cut(s) 1035
AsuHPI GGTGA 4 cut(s) 284, 587, 809, 1012
AvaII GGWCC 1 cut(s) 105
BaeGI GKGCMC 2 cut(s) 1035, 1081
BalI TGGCCA 3 cut(s) 378, 405, 1013
BanII GRGCYC 1 cut(s) 353
BbsI GAAGAC 2 cut(s) 558, 837
Bbv12I GWGCWC 1 cut(s) 1081
BbvI GCAGC 1 cut(s) 496
BccI CCATC 3 cut(s) 521, 997, 1022
BceAI ACGGC 3 cut(s) 35, 615, 839
BciVI GTATCC 1 cut(s) 776
BclI TGATCA 1 cut(s) 799
BcnI CCSGG 1 cut(s) 1035
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 1 cut(s) 440
BfmI CTRYAG 2 cut(s) 567, 1136
BfuI GTATCC 1 cut(s) 776
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 2 cut(s) 24, 510
BlsI GCNGC 2 cut(s) 25, 511
Bme1390I CCNGG 1 cut(s) 1035
Bme18I GGWCC 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 105
BmiI GGNNCC 2 cut(s) 33, 106
BmrFI CCNGG 1 cut(s) 1035
BmrI ACTGGG 3 cut(s) 189, 706, 971
BmsI GCATC 1 cut(s) 245
BmuI ACTGGG 3 cut(s) 189, 706, 971
BpiI GAAGAC 2 cut(s) 558, 837
BplI GAGNNNNNCTC 2 cut(s) 803, 835
Bpu10I CCTNAGC 1 cut(s) 732
BpuMI CCSGG 1 cut(s) 1035
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 3 cut(s) 51, 406, 1155
BsaWI WCCGGW 1 cut(s) 107
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse118I RCCGGY 1 cut(s) 445
Bse1I ACTGG 3 cut(s) 184, 712, 966
Bse3DI GCAATG 2 cut(s) 224, 999
BseDI CCNNGG 3 cut(s) 51, 406, 1155
BseGI GGATG 1 cut(s) 678
BseLI CCNNNNNNNGG 1 cut(s) 635
BseMI GCAATG 2 cut(s) 224, 999
BseMII CTCAG 2 cut(s) 353, 746
BseNI ACTGG 3 cut(s) 184, 712, 966
BseRI GAGGAG 1 cut(s) 197
BseSI GKGCMC 2 cut(s) 1035, 1081
BseXI GCAGC 1 cut(s) 496
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 5 cut(s) 50, 120, 378, 405, 1013
BsiHKAI GWGCWC 1 cut(s) 1081
BsiSI CCGG 3 cut(s) 108, 446, 1035
BslFI GGGAC 1 cut(s) 118
BslI CCNNNNNNNGG 1 cut(s) 635
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 118
BsnI GGCC 5 cut(s) 50, 120, 378, 405, 1013
Bsp1286I GDGCHC 3 cut(s) 353, 1035, 1081
Bsp143I GATC 5 cut(s) 183, 603, 799, 897, 921
Bsp19I CCATGG 1 cut(s) 406
BspACI CCGC 6 cut(s) 5, 9, 23, 26, 345, 1193
BspANI GGCC 5 cut(s) 50, 120, 378, 405, 1013
BspCNI CTCAG 2 cut(s) 352, 745
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 2 cut(s) 33, 106
BspPI GGATC 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 224, 999
BsrFI RCCGGY 1 cut(s) 445
BsrI ACTGG 3 cut(s) 184, 712, 966
BssAI RCCGGY 1 cut(s) 445
BssECI CCNNGG 3 cut(s) 51, 406, 1155
BssMI GATC 5 cut(s) 183, 603, 799, 897, 921
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 2 cut(s) 406, 1155
Bst4CI ACNGT 4 cut(s) 797, 1072, 1140, 1176
BstACI GRCGYC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 1049
BstC8I GCNNGC 8 cut(s) 7, 153, 157, 161, 203, 564, 729, 1101
BstDEI CTNAG 2 cut(s) 339, 732
BstDSI CCRYGG 2 cut(s) 51, 406
BstF5I GGATG 1 cut(s) 678
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 5 cut(s) 186, 606, 802, 900, 924
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 5 cut(s) 183, 603, 799, 897, 921
BstMWI GCNNNNNNNGC 5 cut(s) 56, 165, 689, 1019, 1049
BstNSI RCATGY 1 cut(s) 205
BstSCI CCNGG 1 cut(s) 1033
BstSFI CTRYAG 2 cut(s) 567, 1136
BstSLI GKGCMC 2 cut(s) 1035, 1081
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 1 cut(s) 496
BstV2I GAAGAC 2 cut(s) 558, 837
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BsuI GTATCC 1 cut(s) 776
BsuRI GGCC 5 cut(s) 50, 120, 378, 405, 1013
BtgI CCRYGG 2 cut(s) 51, 406
BtsCI GGATG 1 cut(s) 678
BtsI GCAGTG 2 cut(s) 654, 1050
BtsIMutI CAGTG 6 cut(s) 177, 258, 347, 654, 1050, 1079
Cac8I GCNNGC 8 cut(s) 7, 153, 157, 161, 203, 564, 729, 1101
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 1 cut(s) 445
Cfr13I GGNCC 1 cut(s) 105
CseI GACGC 2 cut(s) 82, 157
Csp6I GTAC 2 cut(s) 1068, 1203
CviAII CATG 6 cut(s) 202, 407, 787, 901, 982, 1026
CviQI GTAC 2 cut(s) 1068, 1203
DdeI CTNAG 2 cut(s) 339, 732
DpnI GATC 5 cut(s) 185, 605, 801, 899, 923
DpnII GATC 5 cut(s) 183, 603, 799, 897, 921
DraI TTTAAA 1 cut(s) 1116
EaeI YGGCCR 3 cut(s) 376, 403, 1011
Eco130I CCWWGG 2 cut(s) 406, 1155
Eco24I GRGCYC 1 cut(s) 353
Eco32I GATATC 1 cut(s) 1086
Eco47I GGWCC 1 cut(s) 105
Eco57I CTGAAG 1 cut(s) 481
EcoRV GATATC 1 cut(s) 1086
EcoT14I CCWWGG 2 cut(s) 406, 1155
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 2 cut(s) 406, 1155
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 6 cut(s) 205, 410, 790, 904, 985, 1029
FaqI GGGAC 1 cut(s) 118
FatI CATG 6 cut(s) 201, 406, 786, 900, 981, 1025
FauI CCCGC 2 cut(s) 19, 338
FbaI TGATCA 1 cut(s) 799
FblI GTMKAC 1 cut(s) 759
Fnu4HI GCNGC 2 cut(s) 24, 510
FokI GGATG 1 cut(s) 685
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 24, 510
FspBI CTAG 1 cut(s) 440
GlaI GCGC 1 cut(s) 22
GluI GCNGC 2 cut(s) 24, 510
HaeIII GGCC 5 cut(s) 50, 120, 378, 405, 1013
HapII CCGG 3 cut(s) 108, 446, 1035
HgaI GACGC 2 cut(s) 82, 157
HhaI GCGC 1 cut(s) 23
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 6 cut(s) 205, 410, 790, 904, 985, 1029
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HinfI GANTC 7 cut(s) 138, 248, 320, 411, 774, 947, 985
HpaII CCGG 3 cut(s) 108, 446, 1035
HphI GGTGA 4 cut(s) 284, 587, 809, 1012
Hpy166II GTNNAC 3 cut(s) 14, 760, 1079
Hpy188I TCNGA 5 cut(s) 72, 80, 241, 315, 861
Hpy188III TCNNGA 2 cut(s) 670, 895
Hpy8I GTNNAC 3 cut(s) 14, 760, 1079
HpyAV CCTTC 3 cut(s) 131, 291, 505
HpyCH4III ACNGT 4 cut(s) 797, 1072, 1140, 1176
HpyCH4V TGCA 9 cut(s) 201, 217, 222, 236, 429, 519, 647, 1043, 1079
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 165, 689, 1019, 1049
HpyF3I CTNAG 2 cut(s) 339, 732
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 6 cut(s) 205, 410, 790, 904, 985, 1029
HspAI GCGC 1 cut(s) 21
Ksp22I TGATCA 1 cut(s) 799
Kzo9I GATC 5 cut(s) 183, 603, 799, 897, 921
LmnI GCTCC 3 cut(s) 210, 496, 909
Lsp1109I GCAGC 1 cut(s) 496
LweI GCATC 1 cut(s) 245
MaeI CTAG 1 cut(s) 440
MaeIII GTNAC 1 cut(s) 175
MalI GATC 5 cut(s) 185, 605, 801, 899, 923
MboI GATC 5 cut(s) 183, 603, 799, 897, 921
MboII GAAGA 7 cut(s) 254, 257, 563, 837, 1009, 1122, 1156
MfeI CAATTG 1 cut(s) 537
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 3 cut(s) 353, 1035, 1081
MlsI TGGCCA 3 cut(s) 378, 405, 1013
MluCI AATT 8 cut(s) 421, 453, 520, 537, 722, 838, 968, 1118
MluNI TGGCCA 3 cut(s) 378, 405, 1013
MlyI GAGTC 1 cut(s) 994
Mox20I TGGCCA 3 cut(s) 378, 405, 1013
Mph1103I ATGCAT 1 cut(s) 238
MscI TGGCCA 3 cut(s) 378, 405, 1013
MseI TTAA 3 cut(s) 258, 420, 1115
Msp20I TGGCCA 3 cut(s) 378, 405, 1013
MspI CCGG 3 cut(s) 108, 446, 1035
MspR9I CCNGG 1 cut(s) 1035
MunI CAATTG 1 cut(s) 537
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 5 cut(s) 56, 165, 689, 1019, 1049
NciI CCSGG 1 cut(s) 1035
NcoI CCATGG 1 cut(s) 406
NdeII GATC 5 cut(s) 183, 603, 799, 897, 921
NlaIII CATG 6 cut(s) 205, 410, 790, 904, 985, 1029
NlaIV GGNNCC 2 cut(s) 33, 106
NmuCI GTSAC 1 cut(s) 175
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 1 cut(s) 205
PaeI GCATGC 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 639
PfeI GAWTC 6 cut(s) 138, 248, 320, 411, 774, 947
PkrI GCNGC 2 cut(s) 25, 511
PleI GAGTC 1 cut(s) 993
PpsI GAGTC 1 cut(s) 993
PspN4I GGNNCC 2 cut(s) 33, 106
PspPI GGNCC 1 cut(s) 105
PsuI RGATCY 1 cut(s) 183
RsaI GTAC 2 cut(s) 1069, 1204
RsaNI GTAC 2 cut(s) 1068, 1203
SaqAI TTAA 3 cut(s) 258, 420, 1115
SatI GCNGC 2 cut(s) 24, 510
Sau3AI GATC 5 cut(s) 183, 603, 799, 897, 921
Sau96I GGNCC 1 cut(s) 105
SchI GAGTC 1 cut(s) 994
ScrFI CCNGG 1 cut(s) 1035
SduI GDGCHC 3 cut(s) 353, 1035, 1081
SetI ASST 5 cut(s) 194, 307, 475, 493, 568
SfaNI GCATC 1 cut(s) 245
SfcI CTRYAG 2 cut(s) 567, 1136
SinI GGWCC 1 cut(s) 105
SphI GCATGC 1 cut(s) 205
Sse9I AATT 8 cut(s) 421, 453, 520, 537, 722, 838, 968, 1118
SsiI CCGC 6 cut(s) 5, 9, 23, 26, 345, 1193
SspMI CTAG 1 cut(s) 440
StyD4I CCNGG 1 cut(s) 1033
StyI CCWWGG 2 cut(s) 406, 1155
TaaI ACNGT 4 cut(s) 797, 1072, 1140, 1176
TaqI TCGA 2 cut(s) 62, 988
TaqII GACCGA 1 cut(s) 332
TasI AATT 8 cut(s) 421, 453, 520, 537, 722, 838, 968, 1118
TatI WGTACW 1 cut(s) 1067
TauI GCSGC 1 cut(s) 26
TfiI GAWTC 6 cut(s) 138, 248, 320, 411, 774, 947
Tru1I TTAA 3 cut(s) 258, 420, 1115
Tru9I TTAA 3 cut(s) 258, 420, 1115
TscAI CASTG 6 cut(s) 184, 258, 347, 654, 1050, 1086
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 1 cut(s) 509
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 6 cut(s) 300, 378, 713, 939, 1100, 1156
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 6 cut(s) 184, 258, 347, 654, 1050, 1086
VneI GTGCAC 1 cut(s) 1077
VpaK11BI GGWCC 1 cut(s) 105
XceI RCATGY 1 cut(s) 205
XmiI GTMKAC 1 cut(s) 759
XspI CTAG 1 cut(s) 440
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.