Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012445

Physical Location & Seq

Genomic Coordinates Forward (+)

5411944 .. 5415340

Gene Structure 3397 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1047000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGAAGGTGGAGGGGGAGAAAGCAAGGGGGTTCCTCTGCTGGACAGGCCGGCTGGGGCTTCGACGGCGCAGACGCTCGGAAATATTATTGTTTCGGTTGTCGGCACTGGCGTTTTGGGCCTCCCCTTCGCTTTGAGAATCGCCGGTTGGCTCGCTGGTTCTCTTGGGGTCATGGTTGCTGGTCTCTCCACCTACTACTGCATGCTCCTCCTTGTGCAATGTAGGGATAAGTTGGCATCAGAAGAAAATTCATCAGGTAAAAAAACTTATGGTGATTTGGGGCATGGATGCATGGGAAGAACAGGTCGTTATCTAACCGAATCCTTGATTCTGATTGCCCAATGTGGAGGTTCCGTGGCGTACCTTGTATTTATTGGACAAAACCTTTCGTCGATGTTGAATGGCCACGGTTTCTCAGTTGCTTCCTGTATATTTTTATTAGTTCCAGTCGAAATTGGGTTGTCTTGGATAGGCAGTCTATCAGCTTTAGCACCCTTCAGTGTGTTTGCGTCAGTATGCAATGTGTTGGCTATGGCGTTTGTGGTGAAAGAAGACATACAGCAGGCAGTGGAGGGCGAGTTTTCTTTTAGGGATAGGACAGCGATCACATCAAGTATAGGAGGGTTGCCATTTGCAGGAGGTGTGGCGGTGTTCTGTTTTGAGGGGTTTGGGATGACATTAGCACTGGAAGGTTCTATGCGAGATAAAAGTACATTCCCGAGGTTGCTTGCTCAGGCTTTCACAGGGATAACCCTTGTCTATTGTTTATTTGGATTTGCCGGTTACATGGCTTATGGTGATCAAACCCTCGAAATTGTCACTCTCAATCTCCCTCACAATTGGTCTGCCATGGTGGTTCAGATTGGTTTGTGCTTGGGGCTAATATTTACCTTCCCAATCATGCTACACCCGATAAACGAGATCATAGAAGGGAAGTTGGTAAAAGGCAAATGGTTTCAGAAAGCTCATGATGACAATGACGATTATTCAACGATGCCGCCTCTAGGAAGGTTTGCAGTGTACGTGAGTCGAGCAATAGTGGTGATCATACTGGCCGTCTTGGCCTCGTGCGTTCCAGGGTTTGCAGTATTTGCGTCGCTCGTGGGAAGTACTGTATGTGCACTGATCTCATTTGTTCTACCTGCTTCATTTCACTTAGCATTGTTGGGTTCTTCCCTAAAATTGTGGCAAAGAACCTTGGATTGTTGCATATTATTTTGCGGAATGCTTTTTGCCGCATACGGCGCTTACAATGCCGTAGTTGGCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0009605 GO:0009607 GO:0009624 GO:0012505 GO:0015075 GO:0015171 GO:0015173 GO:0015175 GO:0015318 GO:0015711 GO:0015801 GO:0015804 GO:0015849 GO:0016020 GO:0022857 GO:0031984 GO:0034220 GO:0042175 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0071702 GO:0071705 GO:0098656 GO:0098827 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

Aa_trans

Protein Analysis

424

Amino Acids

44.73

Weight (kDa)

6.07

Isoelectric Point (pI)

30.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aa_trans	PF01490	20 - 420	4.6e-93	Transmembrane amino acid transporter protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012445)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11900 AT3G11900
fragaria_vesca	FvH4_6g21140 FvH4_6g21140 FvH4_6g21140
malus_domestica	MD12G1047000.v1.1
prunus_persica	Prupe.7G088200_v2.0.a1 Prupe.7G088200_v2.0.a1
pyrus_communis	pycom12g04050
rosa_chinensis	RchiOBHm_Chr3g0477611
rosa_laevigata	RLG00000023706
rosa_multiflora	Rmu_sc0000775.1_g000010
rosa_roxburghii	Rroxscaffold_6G00403830
rosa_rugosa	Rorug03G0162300
rosa_samantha	Rh3AG212300 Rh3BG246000 Rh3CG239600 Rh3DG239100
rosa_wichuraiana	Rw3G019220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1150
AciI	CCGC	4 cut(s)	647, 998, 1221, 1236
AcoI	YGGCCR	2 cut(s)	403, 1053
AcsI	RAATTY	1 cut(s)	247
AcuI	CTGAAG	1 cut(s)	481
AfaI	GTAC	4 cut(s)	362, 712, 1022, 1111
AfiI	CCNNNNNNNGG	2 cut(s)	635, 1004
AgsI	TTSAA	2 cut(s)	400, 990
AjnI	CCWGG	1 cut(s)	1075
AjuI	GAANNNNNNNTTGG	2 cut(s)	130, 162
AluBI	AGCT	2 cut(s)	485, 965
AluI	AGCT	2 cut(s)	485, 965
Alw21I	GWGCWC	1 cut(s)	1123
Alw26I	GTCTC	1 cut(s)	188
Alw44I	GTGCAC	1 cut(s)	1119
Ama87I	CYCGRG	1 cut(s)	718
AoxI	GGCC	5 cut(s)	47, 118, 403, 1053, 1062
ApaLI	GTGCAC	1 cut(s)	1119
ApoI	RAATTY	1 cut(s)	247
AspLEI	GCGC	2 cut(s)	70, 1247
AspS9I	GGNCC	1 cut(s)	118
AsuHPI	GGTGA	4 cut(s)	284, 556, 809, 1054
AvaI	CYCGRG	1 cut(s)	718
BaeGI	GKGCMC	1 cut(s)	1123
BalI	TGGCCA	1 cut(s)	405
BauI	CACGAG	2 cut(s)	1066, 1100
BbsI	GAAGAC	1 cut(s)	558
Bbv12I	GWGCWC	1 cut(s)	1123
BceAI	ACGGC	4 cut(s)	81, 1040, 1241, 1258
BciT130I	CCWGG	1 cut(s)	1077
BclI	TGATCA	2 cut(s)	799, 1044
BcoDI	GTCTC	1 cut(s)	188
BfaI	CTAG	1 cut(s)	1004
BfoI	RGCGCY	1 cut(s)	1248
BfuAI	ACCTGC	1 cut(s)	1150
BglI	GCCNNNNNGGC	1 cut(s)	1061
BisI	GCNGC	2 cut(s)	998, 1236
BlsI	GCNGC	2 cut(s)	999, 1237
BmcAI	AGTACT	1 cut(s)	1111
Bme1390I	CCNGG	1 cut(s)	1077
BmeT110I	CYCGRG	1 cut(s)	718
BmgT120I	GGNCC	1 cut(s)	118
BmiI	GGNNCC	2 cut(s)	33, 352
BmrFI	CCNGG	1 cut(s)	1077
BmsI	GCATC	3 cut(s)	245, 278, 984
BpiI	GAAGAC	1 cut(s)	558
Bpu10I	CCTNAGC	1 cut(s)	732
BsaAI	YACGTR	1 cut(s)	1024
BsaI	GGTCTC	1 cut(s)	188
BsaJI	CCNNGG	6 cut(s)	354, 406, 719, 849, 1076, 1197
BsaXI	ACNNNNNCTCC	4 cut(s)	339, 369, 563, 593
Bsc4I	CCNNNNNNNGG	2 cut(s)	635, 1004
Bse118I	RCCGGY	3 cut(s)	49, 143, 779
Bse1I	ACTGG	4 cut(s)	112, 446, 690, 1056
Bse3DI	GCAATG	2 cut(s)	224, 526
BseBI	CCWGG	1 cut(s)	1077
BseDI	CCNNGG	6 cut(s)	354, 406, 719, 849, 1076, 1197
BseGI	GGATG	2 cut(s)	293, 678
BseLI	CCNNNNNNNGG	2 cut(s)	635, 1004
BseMI	GCAATG	2 cut(s)	224, 526
BseMII	CTCAG	2 cut(s)	429, 746
BseNI	ACTGG	4 cut(s)	112, 446, 690, 1056
BseRI	GAGGAG	1 cut(s)	197
BseSI	GKGCMC	1 cut(s)	1123
BseYI	CCCAGC	1 cut(s)	53
BshFI	GGCC	5 cut(s)	49, 120, 405, 1055, 1064
BsiHKAI	GWGCWC	1 cut(s)	1123
BsiHKCI	CYCGRG	1 cut(s)	718
BsiSI	CCGG	3 cut(s)	50, 144, 780
BslI	CCNNNNNNNGG	2 cut(s)	635, 1004
BsmAI	GTCTC	1 cut(s)	188
BsmI	GAATGC	1 cut(s)	1230
BsnI	GGCC	5 cut(s)	49, 120, 405, 1055, 1064
Bso31I	GGTCTC	1 cut(s)	188
BsoBI	CYCGRG	1 cut(s)	718
Bsp1286I	GDGCHC	1 cut(s)	1123
Bsp143I	GATC	5 cut(s)	603, 799, 921, 1044, 1125
Bsp19I	CCATGG	1 cut(s)	849
BspACI	CCGC	4 cut(s)	647, 998, 1221, 1236
BspANI	GGCC	5 cut(s)	49, 120, 405, 1055, 1064
BspCNI	CTCAG	2 cut(s)	428, 745
BspHI	TCATGA	1 cut(s)	967
BspLI	GGNNCC	2 cut(s)	33, 352
BspMI	ACCTGC	1 cut(s)	1150
BspTNI	GGTCTC	1 cut(s)	188
BsrDI	GCAATG	2 cut(s)	224, 526
BsrFI	RCCGGY	3 cut(s)	49, 143, 779
BsrI	ACTGG	4 cut(s)	112, 446, 690, 1056
BssAI	RCCGGY	3 cut(s)	49, 143, 779
BssECI	CCNNGG	6 cut(s)	354, 406, 719, 849, 1076, 1197
BssMI	GATC	5 cut(s)	603, 799, 921, 1044, 1125
BssSI	CACGAG	2 cut(s)	1066, 1100
BssT1I	CCWWGG	2 cut(s)	849, 1197
Bst2BI	CACGAG	2 cut(s)	1066, 1100
Bst2UI	CCWGG	1 cut(s)	1077
Bst4CI	ACNGT	2 cut(s)	410, 1114
BstAPI	GCANNNNNTGC	1 cut(s)	1091
BstBAI	YACGTR	1 cut(s)	1024
BstC8I	GCNNGC	5 cut(s)	51, 153, 203, 564, 729
BstDEI	CTNAG	3 cut(s)	415, 732, 1156
BstDSI	CCRYGG	3 cut(s)	354, 406, 849
BstF5I	GGATG	2 cut(s)	293, 678
BstH2I	RGCGCY	1 cut(s)	1248
BstHHI	GCGC	2 cut(s)	70, 1247
BstKTI	GATC	5 cut(s)	606, 802, 924, 1047, 1128
BstMAI	GTCTC	1 cut(s)	188
BstMBI	GATC	5 cut(s)	603, 799, 921, 1044, 1125
BstMWI	GCNNNNNNNGC	7 cut(s)	46, 65, 117, 1061, 1091, 1244, 1253
BstNI	CCWGG	1 cut(s)	1077
BstNSI	RCATGY	1 cut(s)	205
BstSCI	CCNGG	1 cut(s)	1075
BstSLI	GKGCMC	1 cut(s)	1123
BstV2I	GAAGAC	1 cut(s)	558
BsuRI	GGCC	5 cut(s)	49, 120, 405, 1055, 1064
BtgI	CCRYGG	3 cut(s)	354, 406, 849
BtsCI	GGATG	2 cut(s)	293, 678
BtsI	GCAGTG	2 cut(s)	573, 1023
BtsIMutI	CAGTG	6 cut(s)	105, 505, 573, 683, 1023, 1121
BveI	ACCTGC	1 cut(s)	1150
Cac8I	GCNNGC	5 cut(s)	51, 153, 203, 564, 729
CciI	TCATGA	1 cut(s)	967
CfoI	GCGC	2 cut(s)	70, 1247
Cfr10I	RCCGGY	3 cut(s)	49, 143, 779
Cfr13I	GGNCC	1 cut(s)	118
CseI	GACGC	3 cut(s)	82, 498, 1083
Csp6I	GTAC	4 cut(s)	361, 711, 1021, 1110
CviAII	CATG	8 cut(s)	172, 202, 284, 292, 787, 850, 901, 968
CviQI	GTAC	4 cut(s)	361, 711, 1021, 1110
DdeI	CTNAG	3 cut(s)	415, 732, 1156
DpnI	GATC	5 cut(s)	605, 801, 923, 1046, 1127
DpnII	GATC	5 cut(s)	603, 799, 921, 1044, 1125
EaeI	YGGCCR	2 cut(s)	403, 1053
Eco130I	CCWWGG	2 cut(s)	849, 1197
Eco31I	GGTCTC	1 cut(s)	188
Eco57I	CTGAAG	1 cut(s)	481
Eco88I	CYCGRG	1 cut(s)	718
EcoRII	CCWGG	1 cut(s)	1075
EcoT14I	CCWWGG	2 cut(s)	849, 1197
EcoT22I	ATGCAT	1 cut(s)	293
ErhI	CCWWGG	2 cut(s)	849, 1197
FaeI	CATG	8 cut(s)	175, 205, 287, 295, 790, 853, 904, 971
FatI	CATG	8 cut(s)	171, 201, 283, 291, 786, 849, 900, 967
FbaI	TGATCA	2 cut(s)	799, 1044
Fnu4HI	GCNGC	2 cut(s)	998, 1236
FokI	GGATG	2 cut(s)	300, 685
Fsp4HI	GCNGC	2 cut(s)	998, 1236
FspBI	CTAG	1 cut(s)	1004
GlaI	GCGC	2 cut(s)	69, 1246
GluI	GCNGC	2 cut(s)	998, 1236
GsaI	CCCAGC	1 cut(s)	57
HaeII	RGCGCY	1 cut(s)	1248
HaeIII	GGCC	5 cut(s)	49, 120, 405, 1055, 1064
HapII	CCGG	3 cut(s)	50, 144, 780
HgaI	GACGC	3 cut(s)	82, 498, 1083
HhaI	GCGC	2 cut(s)	70, 1247
Hin1II	CATG	8 cut(s)	175, 205, 287, 295, 790, 853, 904, 971
Hin6I	GCGC	2 cut(s)	68, 1245
HinP1I	GCGC	2 cut(s)	68, 1245
HinfI	GANTC	4 cut(s)	138, 320, 328, 1027
HpaII	CCGG	3 cut(s)	50, 144, 780
HphI	GGTGA	4 cut(s)	284, 556, 809, 1054
Hpy166II	GTNNAC	2 cut(s)	1021, 1121
Hpy188I	TCNGA	5 cut(s)	80, 241, 333, 861, 960
Hpy188III	TCNNGA	2 cut(s)	718, 968
Hpy8I	GTNNAC	2 cut(s)	1021, 1121
Hpy99I	CGWCG	3 cut(s)	67, 394, 1099
HpyAV	CCTTC	6 cut(s)	136, 505, 683, 901, 923, 1002
HpyCH4III	ACNGT	2 cut(s)	410, 1114
HpyCH4IV	ACGT	1 cut(s)	1023
HpyCH4V	TGCA	9 cut(s)	201, 217, 291, 519, 635, 1016, 1085, 1121, 1209
HpyF10VI	GCNNNNNNNGC	7 cut(s)	46, 65, 117, 1061, 1091, 1244, 1253
HpyF3I	CTNAG	3 cut(s)	415, 732, 1156
HpySE526I	ACGT	1 cut(s)	1023
Hsp92II	CATG	8 cut(s)	175, 205, 287, 295, 790, 853, 904, 971
HspAI	GCGC	2 cut(s)	68, 1245
KroI	GCCGGC	1 cut(s)	49
KroNI	GCCGGC	1 cut(s)	51
Ksp22I	TGATCA	2 cut(s)	799, 1044
Kzo9I	GATC	5 cut(s)	603, 799, 921, 1044, 1125
LmnI	GCTCC	1 cut(s)	210
LweI	GCATC	3 cut(s)	245, 278, 984
MaeI	CTAG	1 cut(s)	1004
MaeII	ACGT	1 cut(s)	1023
MaeIII	GTNAC	2 cut(s)	782, 817
MalI	GATC	5 cut(s)	605, 801, 923, 1046, 1127
MboI	GATC	5 cut(s)	603, 799, 921, 1044, 1125
MboII	GAAGA	4 cut(s)	254, 309, 563, 1164
MfeI	CAATTG	1 cut(s)	838
MhlI	GDGCHC	1 cut(s)	1123
MlsI	TGGCCA	1 cut(s)	405
MluCI	AATT	5 cut(s)	247, 453, 813, 838, 1181
MluNI	TGGCCA	1 cut(s)	405
MlyI	GAGTC	1 cut(s)	1036
Mox20I	TGGCCA	1 cut(s)	405
Mph1103I	ATGCAT	1 cut(s)	293
MroNI	GCCGGC	1 cut(s)	49
MscI	TGGCCA	1 cut(s)	405
Msp20I	TGGCCA	1 cut(s)	405
MspI	CCGG	3 cut(s)	50, 144, 780
MspR9I	CCNGG	1 cut(s)	1077
MunI	CAATTG	1 cut(s)	838
Mva1269I	GAATGC	1 cut(s)	1230
MvaI	CCWGG	1 cut(s)	1077
MwoI	GCNNNNNNNGC	7 cut(s)	46, 65, 117, 1061, 1091, 1244, 1253
NaeI	GCCGGC	1 cut(s)	51
NcoI	CCATGG	1 cut(s)	849
NdeII	GATC	5 cut(s)	603, 799, 921, 1044, 1125
NgoMIV	GCCGGC	1 cut(s)	49
NlaIII	CATG	8 cut(s)	175, 205, 287, 295, 790, 853, 904, 971
NlaIV	GGNNCC	2 cut(s)	33, 352
NmuCI	GTSAC	1 cut(s)	817
NsiI	ATGCAT	1 cut(s)	293
NspI	RCATGY	1 cut(s)	205
PaeI	GCATGC	1 cut(s)	205
PagI	TCATGA	1 cut(s)	967
PcsI	WCGNNNNNNNCGW	1 cut(s)	108
PctI	GAATGC	1 cut(s)	1230
PdiI	GCCGGC	1 cut(s)	51
PfeI	GAWTC	3 cut(s)	138, 320, 328
PkrI	GCNGC	2 cut(s)	999, 1237
PleI	GAGTC	1 cut(s)	1035
PpsI	GAGTC	1 cut(s)	1035
Ppu21I	YACGTR	1 cut(s)	1024
Psp6I	CCWGG	1 cut(s)	1075
PspFI	CCCAGC	1 cut(s)	53
PspGI	CCWGG	1 cut(s)	1075
PspN4I	GGNNCC	2 cut(s)	33, 352
PspPI	GGNCC	1 cut(s)	118
RsaI	GTAC	4 cut(s)	362, 712, 1022, 1111
RsaNI	GTAC	4 cut(s)	361, 711, 1021, 1110
SatI	GCNGC	2 cut(s)	998, 1236
Sau3AI	GATC	5 cut(s)	603, 799, 921, 1044, 1125
Sau96I	GGNCC	1 cut(s)	118
ScaI	AGTACT	1 cut(s)	1111
SchI	GAGTC	1 cut(s)	1036
ScrFI	CCNGG	1 cut(s)	1077
SduI	GDGCHC	1 cut(s)	1123
SfaNI	GCATC	3 cut(s)	245, 278, 984
SfiI	GGCCNNNNNGGCC	1 cut(s)	1061
SphI	GCATGC	1 cut(s)	205
Sse9I	AATT	5 cut(s)	247, 453, 813, 838, 1181
SsiI	CCGC	4 cut(s)	647, 998, 1221, 1236
SspI	AATATT	2 cut(s)	85, 885
SspMI	CTAG	1 cut(s)	1004
StyD4I	CCNGG	1 cut(s)	1075
StyI	CCWWGG	2 cut(s)	849, 1197
TaaI	ACNGT	2 cut(s)	410, 1114
TaiI	ACGT	1 cut(s)	1026
TaqI	TCGA	5 cut(s)	62, 392, 450, 810, 1030
TasI	AATT	5 cut(s)	247, 453, 813, 838, 1181
TatI	WGTACW	2 cut(s)	710, 1109
TauI	GCSGC	2 cut(s)	1000, 1238
TfiI	GAWTC	3 cut(s)	138, 320, 328
TscAI	CASTG	6 cut(s)	112, 505, 573, 690, 1023, 1128
TseFI	GTSAC	1 cut(s)	817
Tsp45I	GTSAC	1 cut(s)	817
TspDTI	ATGAA	2 cut(s)	240, 1137
TspGWI	ACGGA	1 cut(s)	343
TspRI	CASTG	6 cut(s)	112, 505, 573, 690, 1023, 1128
VneI	GTGCAC	1 cut(s)	1119
XapI	RAATTY	1 cut(s)	247
XceI	RCATGY	1 cut(s)	205
XspI	CTAG	1 cut(s)	1004
ZrmI	AGTACT	1 cut(s)	1111
Zsp2I	ATGCAT	1 cut(s)	293

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.