Rorug03G0162300

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0012445
Physical Location & Seq
Forward (+)
13475634 .. 13481049
5416 bp
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UTR
Exon/CDS
Intron
Rorug03G0162300.1

Sequence Viewer

Length: 831 bp
ATGTTTTACAAACGAAACGGTGGTCTATTGCTAGAACAACAATTATCGTCAGGTGATATTAACGTTGAGAGAATCAAATTGTTCAAATGCAAGGAGCTACAGAGGTCTACAAACAATTTCAATGCTGATAGAATTATTGGCCAAGGGGGGCAAGATACTGTTTATAAAGGCATGTTGATGGATGGAAGAATTGTGGCTGTTAAAAAGTCAAAAATTGTAGACAAGGCAAAACTTTCAGAATTCATCAATGAGGTTGTCGTTCTTTCTCAAATCAACCACAGGAACATCGTTCAACGATCGGGTTGTTGTCTAGAGACGGAAGTTCCTCTTTTGGGAACATTTGGTTATTTGGATCCTGAATACTACCAATCAAGCCAATTCACTGAGAAAAGTGACGTCTACAGCTTTGGAGTGGTGCTTGTTGAGCTATTGACCGGAAAAAAACCAGTAACAAGGTTAGCCGAAGACGAAACATATTTTATTGTTTCGTTGCAGGAAGATTCTCTATTTGACATTCTCGATGCTCGAGTTGTGAATGAAGGCTCCAAAGATGGTATTTTGGCAGTCGCTGAGCTTGCAAAACGATGCTTAAATTTGAATGGAAGAAATCGTCCTACTATGAGGGAGATCACAGCAGAGTTGGAGGCCATTCAATTATCAGAGAAACCTGCCGACCCGCATCAACAAAGTTGTGAAAGGGTTGAATTTGGCCAAAATTATGGAATTGAGCAGTGGGATGATGTTGTTTCTAGTTCAACAATGTCAACTAGGGAAGGTTCTCCGACTCCATCATCAGTTGAACTTCCACTAGTTTCTATGAAGACATGTTAA

Protein Analysis

276

Amino Acids

30.9

Weight (kDa)

5.14

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 41 - 105 3.4e-07 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 42 - 107 3e-07 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 111 - 211 1.2e-11 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 116 - 214 1.9e-11 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11900 AT3G11900
fragaria_vesca FvH4_6g21140 FvH4_6g21140 FvH4_6g21140
malus_domestica MD12G1047000.v1.1
prunus_persica Prupe.7G088200_v2.0.a1 Prupe.7G088200_v2.0.a1
pyrus_communis pycom12g04050
rosa_chinensis RchiOBHm_Chr3g0477611
rosa_laevigata RLG00000023706
rosa_multiflora Rmu_sc0000775.1_g000010
rosa_roxburghii Rroxscaffold_6G00403830
rosa_rugosa Rorug03G0162300
rosa_samantha Rh3AG212300 Rh3BG246000 Rh3CG239600 Rh3DG239100
rosa_wichuraiana Rw3G019220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 165
AatII GACGTC 1 cut(s) 399
Acc36I ACCTGC 1 cut(s) 676
AccI GTMKAC 3 cut(s) 107, 219, 399
AciI CCGC 1 cut(s) 677
AclI AACGTT 1 cut(s) 63
AclWI GGATC 2 cut(s) 347, 360
AcoI YGGCCR 2 cut(s) 139, 709
AcsI RAATTY 3 cut(s) 239, 592, 704
AcyI GRCGYC 1 cut(s) 396
AfiI CCNNNNNNNGG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 824
AgsI TTSAA 8 cut(s) 85, 121, 293, 598, 653, 704, 756, 800
AhlI ACTAGT 1 cut(s) 808
AluBI AGCT 4 cut(s) 97, 405, 427, 574
AluI AGCT 4 cut(s) 97, 405, 427, 574
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 2 cut(s) 347, 360
AlwNI CAGNNNCTG 1 cut(s) 569
Ama87I CYCGRG 1 cut(s) 525
AoxI GGCC 3 cut(s) 139, 645, 709
ApoI RAATTY 3 cut(s) 239, 592, 704
ArsI GACNNNNNNTTYG 2 cut(s) 7, 39
AsuHPI GGTGA 1 cut(s) 65
AvaI CYCGRG 1 cut(s) 525
BalI TGGCCA 2 cut(s) 141, 711
BamHI GGATCC 1 cut(s) 352
BbsI GAAGAC 2 cut(s) 471, 827
BccI CCATC 4 cut(s) 172, 176, 545, 796
BcoDI GTCTC 1 cut(s) 308
BcuI ACTAGT 1 cut(s) 808
BfaI CTAG 5 cut(s) 32, 311, 750, 768, 809
BfmI CTRYAG 2 cut(s) 98, 400
BfuAI ACCTGC 1 cut(s) 676
BlpI GCTNAGC 1 cut(s) 570
BmeT110I CYCGRG 1 cut(s) 525
BmiI GGNNCC 2 cut(s) 354, 544
BmsI GCATC 3 cut(s) 511, 575, 688
BpiI GAAGAC 2 cut(s) 471, 827
Bpu1102I GCTNAGC 1 cut(s) 570
BsaHI GRCGYC 1 cut(s) 396
BsaJI CCNNGG 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 434
Bsc4I CCNNNNNNNGG 1 cut(s) 332
Bse1I ACTGG 1 cut(s) 446
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 2 cut(s) 187, 742
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMII CTCAG 2 cut(s) 375, 561
BseNI ACTGG 1 cut(s) 446
Bsh1285I CGRYCG 1 cut(s) 299
BshFI GGCC 3 cut(s) 141, 647, 711
BsiEI CGRYCG 1 cut(s) 299
BsiHKCI CYCGRG 1 cut(s) 525
BsiSI CCGG 1 cut(s) 435
BslI CCNNNNNNNGG 1 cut(s) 332
BsmAI GTCTC 1 cut(s) 308
BsmBI CGTCTC 1 cut(s) 308
BsnI GGCC 3 cut(s) 141, 647, 711
BsoBI CYCGRG 1 cut(s) 525
Bsp143I GATC 3 cut(s) 296, 352, 627
Bsp1720I GCTNAGC 1 cut(s) 570
BspACI CCGC 1 cut(s) 677
BspANI GGCC 3 cut(s) 141, 647, 711
BspCNI CTCAG 2 cut(s) 376, 562
BspLI GGNNCC 2 cut(s) 354, 544
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 2 cut(s) 347, 360
BsrI ACTGG 1 cut(s) 446
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 3 cut(s) 296, 352, 627
BssNI GRCGYC 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 142
Bst4CI ACNGT 2 cut(s) 20, 160
BstACI GRCGYC 1 cut(s) 396
BstC8I GCNNGC 1 cut(s) 576
BstDEI CTNAG 2 cut(s) 384, 570
BstF5I GGATG 2 cut(s) 187, 742
BstKTI GATC 3 cut(s) 299, 355, 630
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 3 cut(s) 296, 352, 627
BstMCI CGRYCG 1 cut(s) 299
BstMWI GCNNNNNNNGC 2 cut(s) 424, 575
BstNSI RCATGY 2 cut(s) 175, 828
BstSFI CTRYAG 2 cut(s) 98, 400
BstV2I GAAGAC 2 cut(s) 471, 827
BstX2I RGATCY 1 cut(s) 352
BstXI CCANNNNNNTGG 1 cut(s) 719
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 3 cut(s) 141, 647, 711
BtsCI GGATG 2 cut(s) 187, 742
BtsI GCAGTG 1 cut(s) 737
BtsIMutI CAGTG 2 cut(s) 381, 737
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 1 cut(s) 576
CaiI CAGNNNCTG 1 cut(s) 569
CviAII CATG 2 cut(s) 172, 825
DdeI CTNAG 2 cut(s) 384, 570
DpnI GATC 3 cut(s) 298, 354, 629
DpnII GATC 3 cut(s) 296, 352, 627
EaeI YGGCCR 2 cut(s) 139, 709
Eco130I CCWWGG 1 cut(s) 142
Eco88I CYCGRG 1 cut(s) 525
EcoRI GAATTC 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 142
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 2 cut(s) 175, 828
FaiI YATR 7 cut(s) 165, 173, 475, 620, 720, 818, 826
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FatI CATG 2 cut(s) 171, 824
FauI CCCGC 1 cut(s) 684
FblI GTMKAC 3 cut(s) 107, 219, 399
FokI GGATG 2 cut(s) 194, 749
FspBI CTAG 5 cut(s) 32, 311, 750, 768, 809
HaeIII GGCC 3 cut(s) 141, 647, 711
HapII CCGG 1 cut(s) 435
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 2 cut(s) 175, 828
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HinfI GANTC 3 cut(s) 72, 500, 784
HpaII CCGG 1 cut(s) 435
HphI GGTGA 1 cut(s) 65
Hpy166II GTNNAC 4 cut(s) 108, 220, 400, 765
Hpy188I TCNGA 3 cut(s) 238, 661, 783
Hpy188III TCNNGA 3 cut(s) 311, 356, 518
Hpy8I GTNNAC 4 cut(s) 108, 220, 400, 765
HpyAV CCTTC 2 cut(s) 533, 767
HpyCH4III ACNGT 2 cut(s) 20, 160
HpyCH4IV ACGT 2 cut(s) 63, 396
HpyCH4V TGCA 3 cut(s) 90, 493, 578
HpyF10VI GCNNNNNNNGC 2 cut(s) 424, 575
HpyF3I CTNAG 2 cut(s) 384, 570
HpySE526I ACGT 2 cut(s) 63, 396
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 2 cut(s) 175, 828
Kzo9I GATC 3 cut(s) 296, 352, 627
LmnI GCTCC 2 cut(s) 94, 548
LpnPI CCDG 7 cut(s) 36, 265, 369, 448, 459, 479, 681
LweI GCATC 3 cut(s) 511, 575, 688
MaeI CTAG 5 cut(s) 32, 311, 750, 768, 809
MaeII ACGT 2 cut(s) 63, 396
MaeIII GTNAC 2 cut(s) 392, 448
MalI GATC 3 cut(s) 298, 354, 629
MboI GATC 3 cut(s) 296, 352, 627
MboII GAAGA 4 cut(s) 198, 476, 509, 615
MflI RGATCY 1 cut(s) 352
MlsI TGGCCA 2 cut(s) 141, 711
MluNI TGGCCA 2 cut(s) 141, 711
MlyI GAGTC 1 cut(s) 778
MmeI TCCRAC 2 cut(s) 621, 806
MnlI CCTC 5 cut(s) 96, 244, 336, 615, 637
Mox20I TGGCCA 2 cut(s) 141, 711
MscI TGGCCA 2 cut(s) 141, 711
MseI TTAA 4 cut(s) 60, 201, 590, 829
MslI CAYNNNNRTG 1 cut(s) 176
Msp20I TGGCCA 2 cut(s) 141, 711
MspI CCGG 1 cut(s) 435
MwoI GCNNNNNNNGC 2 cut(s) 424, 575
NdeII GATC 3 cut(s) 296, 352, 627
NlaIII CATG 2 cut(s) 175, 828
NlaIV GGNNCC 2 cut(s) 354, 544
NmuCI GTSAC 1 cut(s) 392
NspI RCATGY 2 cut(s) 175, 828
PaeR7I CTCGAG 1 cut(s) 525
PciI ACATGT 1 cut(s) 824
PfeI GAWTC 2 cut(s) 72, 500
Ple19I CGATCG 1 cut(s) 299
PleI GAGTC 1 cut(s) 778
PpsI GAGTC 1 cut(s) 778
PscI ACATGT 1 cut(s) 824
PsiI TTATAA 1 cut(s) 165
Psp1406I AACGTT 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 354, 544
PspXI VCTCGAGB 1 cut(s) 525
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 1 cut(s) 352
PvuI CGATCG 1 cut(s) 299
RseI CAYNNNNRTG 1 cut(s) 176
SaqAI TTAA 4 cut(s) 60, 201, 590, 829
Sau3AI GATC 3 cut(s) 296, 352, 627
SchI GAGTC 1 cut(s) 778
SfaNI GCATC 3 cut(s) 511, 575, 688
SfcI CTRYAG 2 cut(s) 98, 400
Sfr274I CTCGAG 1 cut(s) 525
SlaI CTCGAG 1 cut(s) 525
SmiMI CAYNNNNRTG 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 525
SmoI CTYRAG 1 cut(s) 525
SpeI ACTAGT 1 cut(s) 808
SsiI CCGC 1 cut(s) 677
SspMI CTAG 5 cut(s) 32, 311, 750, 768, 809
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 2 cut(s) 20, 160
TaiI ACGT 2 cut(s) 66, 399
TaqI TCGA 2 cut(s) 519, 526
TfiI GAWTC 2 cut(s) 72, 500
Tru1I TTAA 4 cut(s) 60, 201, 590, 829
Tru9I TTAA 4 cut(s) 60, 201, 590, 829
TscAI CASTG 2 cut(s) 388, 737
TseFI GTSAC 1 cut(s) 392
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 2 cut(s) 232, 552
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 2 cut(s) 388, 737
XapI RAATTY 3 cut(s) 239, 592, 704
XbaI TCTAGA 1 cut(s) 310
XceI RCATGY 2 cut(s) 175, 828
XhoI CTCGAG 1 cut(s) 525
XmiI GTMKAC 3 cut(s) 107, 219, 399
XspI CTAG 5 cut(s) 32, 311, 750, 768, 809
ZraI GACGTC 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.