FvH4_6g25320

U3-containing 90S pre-ribosomal complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012458
Physical Location & Seq
Reverse (-)
19161340 .. 19166092
4753 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g25320.t1

Sequence Viewer

Length: 765 bp
ATGGGGAGCGGGAAAGAAAGGAACAACTCTGTCAAGAAGAAGAACAAGGGCAACCCGCTTGGCCCCAAAACAACCAAACTTAAACCCCATGCCACCAACAACAACAACAAGAATACCCAAATCGAAGCTCAAAACATCGAAAAGCTACAACCGTCGGCACAACTCAACTTGTTCCTCGACAAATTTCAGTCTGCCAATCATCTCAAGCTCTCTTGTCTCGAATTGGACTCCCTCACTGATAAATGTATTGTGGACATGTCTACGGATCAAGATGTCGAAAGCTTGGGGAAACATGTCAAGGCAGCTTTTGGGCCGTCATGGAAGGAGGAACTATGTGATAAGCGTCTCTTGGAAGGAAAAGTCGATCCAGGGAGCCCCGCAGTTCTTATTATTAGCACATCCGCCTTGAGATCAATAGAACTTCTAAGTCTAGACATTTTTCTCTCATCCACTTTAGGCCTTTTCCAGGTCTCATTGTTGAAGGATCGTGTTAACATCGCTAGTGGTACACCAAACAGGATAAAGAGGCTAATTGACATTGAGGCATTGGGGCTGTCACGGTTATCAGTGATTGTGCTTGATGTGCACCCAGATGTCAAGGGCTATTCACTGTTGACGCTTCCGCAAGTCAGTGATGAATTTTGGGACTTGTACAAGACCTACTTTCACCAGCGATTACTCCAAGGGTCCTTGCGTCTCTCTCTTTATGGTCCATTAAAATCTGATAATGAGTTCAAGGGAAAAAAGAAAAGACCCAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMS1

Protein Analysis

255

Amino Acids

28.32

Weight (kDa)

9.29

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMS1 PF14617 160 - 217 5.9e-07 U3-containing 90S pre-ribosomal complex subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43110 AT2G43110
fragaria_vesca FvH4_4g11840 FvH4_6g25320
malus_domestica MD04G1034000.v1.1 MD13G1276400.v1.1
prunus_persica Prupe.1G180600_v2.0.a1
pyrus_communis pycom04g02840
rosa_chinensis RchiOBHm_Chr4g0409281
rosa_laevigata RLG00000008581
rosa_multiflora Rmu_sc0002377.1_g000010
rosa_roxburghii Rroxscaffold_5G00353750 Rroxscaffold_7G00195060
rosa_rugosa Rorug04G0092000
rosa_samantha Rh4AG155000 Rh4BG152700 Rh4CG164000 Rh4DG148100
rosa_wichuraiana Rw4G012660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 9
AccI GTMKAC 1 cut(s) 260
AciI CCGC 5 cut(s) 9, 56, 378, 402, 623
AclWI GGATC 3 cut(s) 273, 359, 492
AcsI RAATTY 2 cut(s) 182, 638
AfaI GTAC 2 cut(s) 508, 653
AfiI CCNNNNNNNGG 1 cut(s) 466
AflIII ACRYGT 2 cut(s) 255, 292
AgsI TTSAA 2 cut(s) 481, 736
AjnI CCWGG 2 cut(s) 367, 465
AluBI AGCT 5 cut(s) 128, 145, 208, 282, 305
AluI AGCT 5 cut(s) 128, 145, 208, 282, 305
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 4 cut(s) 221, 350, 475, 701
Alw44I GTGCAC 1 cut(s) 584
AlwI GGATC 3 cut(s) 273, 359, 492
AoxI GGCC 3 cut(s) 61, 311, 457
ApaLI GTGCAC 1 cut(s) 584
ApeKI GCWGC 1 cut(s) 302
ApoI RAATTY 2 cut(s) 182, 638
AspS9I GGNCC 4 cut(s) 62, 311, 687, 710
AsuHPI GGTGA 1 cut(s) 659
AvaII GGWCC 2 cut(s) 687, 710
BaeGI GKGCMC 1 cut(s) 588
BanII GRGCYC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 588
BbvI GCAGC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 298
BciT130I CCWGG 2 cut(s) 369, 467
BcoDI GTCTC 4 cut(s) 221, 350, 475, 701
BfaI CTAG 2 cut(s) 431, 501
BisI GCNGC 1 cut(s) 303
BlsI GCNGC 1 cut(s) 304
Bme1390I CCNGG 2 cut(s) 369, 467
Bme18I GGWCC 2 cut(s) 687, 710
BmgT120I GGNCC 4 cut(s) 62, 311, 687, 710
BmiI GGNNCC 3 cut(s) 64, 374, 688
BmrFI CCNGG 2 cut(s) 369, 467
BpuEI CTTGAG 2 cut(s) 188, 427
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 2 cut(s) 368, 682
Bsc4I CCNNNNNNNGG 1 cut(s) 466
BseBI CCWGG 2 cut(s) 369, 467
BseDI CCNNGG 2 cut(s) 368, 682
BseGI GGATG 2 cut(s) 398, 446
BseLI CCNNNNNNNGG 1 cut(s) 466
BseSI GKGCMC 1 cut(s) 588
BseXI GCAGC 1 cut(s) 314
BshFI GGCC 3 cut(s) 63, 313, 459
BsiHKAI GWGCWC 1 cut(s) 588
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 4 cut(s) 221, 350, 475, 701
BsmBI CGTCTC 2 cut(s) 350, 701
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 3 cut(s) 63, 313, 459
Bso31I GGTCTC 1 cut(s) 475
Bsp1286I GDGCHC 2 cut(s) 377, 588
Bsp1407I TGTACA 1 cut(s) 651
Bsp143I GATC 4 cut(s) 265, 364, 410, 484
BspACI CCGC 5 cut(s) 9, 56, 378, 402, 623
BspANI GGCC 3 cut(s) 63, 313, 459
BspLI GGNNCC 3 cut(s) 64, 374, 688
BspPI GGATC 3 cut(s) 273, 359, 492
BspTNI GGTCTC 1 cut(s) 475
BsrBI CCGCTC 1 cut(s) 9
BsrGI TGTACA 1 cut(s) 651
BssECI CCNNGG 2 cut(s) 368, 682
BssMI GATC 4 cut(s) 265, 364, 410, 484
BssT1I CCWWGG 1 cut(s) 682
Bst2UI CCWGG 2 cut(s) 369, 467
Bst4CI ACNGT 3 cut(s) 153, 561, 612
BstAUI TGTACA 1 cut(s) 651
BstDEI CTNAG 1 cut(s) 425
BstENI CCTNNNNNAGG 1 cut(s) 464
BstF5I GGATG 2 cut(s) 398, 446
BstKTI GATC 4 cut(s) 268, 367, 413, 487
BstMAI GTCTC 4 cut(s) 221, 350, 475, 701
BstMBI GATC 4 cut(s) 265, 364, 410, 484
BstMWI GCNNNNNNNGC 1 cut(s) 583
BstNI CCWGG 2 cut(s) 369, 467
BstNSI RCATGY 2 cut(s) 259, 296
BstSCI CCNGG 2 cut(s) 367, 465
BstSLI GKGCMC 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 314
BsuRI GGCC 3 cut(s) 63, 313, 459
BtgZI GCGATG 1 cut(s) 481
BtsCI GGATG 2 cut(s) 398, 446
BtsIMutI CAGTG 4 cut(s) 234, 573, 608, 637
Cfr13I GGNCC 4 cut(s) 62, 311, 687, 710
CseI GACGC 3 cut(s) 332, 625, 683
Csp6I GTAC 2 cut(s) 507, 652
CviAII CATG 4 cut(s) 89, 256, 293, 318
CviQI GTAC 2 cut(s) 507, 652
DdeI CTNAG 1 cut(s) 425
DpnI GATC 4 cut(s) 267, 366, 412, 486
DpnII GATC 4 cut(s) 265, 364, 410, 484
EciI GGCGGA 1 cut(s) 391
Eco130I CCWWGG 1 cut(s) 682
Eco147I AGGCCT 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 377
Eco31I GGTCTC 1 cut(s) 475
Eco47I GGWCC 2 cut(s) 687, 710
EcoNI CCTNNNNNAGG 1 cut(s) 464
EcoO109I RGGNCCY 1 cut(s) 687
EcoRII CCWGG 2 cut(s) 367, 465
EcoT14I CCWWGG 1 cut(s) 682
EcoT38I GRGCYC 1 cut(s) 377
ErhI CCWWGG 1 cut(s) 682
Esp3I CGTCTC 2 cut(s) 350, 701
FaeI CATG 4 cut(s) 92, 259, 296, 321
FaiI YATR 6 cut(s) 90, 257, 294, 319, 334, 708
FalI AAGNNNNNCTT 4 cut(s) 332, 364, 647, 679
FaqI GGGAC 1 cut(s) 659
FatI CATG 4 cut(s) 88, 255, 292, 317
FauI CCCGC 3 cut(s) 2, 63, 385
FblI GTMKAC 1 cut(s) 260
Fnu4HI GCNGC 1 cut(s) 303
FokI GGATG 2 cut(s) 385, 433
FriOI GRGCYC 1 cut(s) 377
Fsp4HI GCNGC 1 cut(s) 303
FspBI CTAG 2 cut(s) 431, 501
GluI GCNGC 1 cut(s) 303
HaeIII GGCC 3 cut(s) 63, 313, 459
HgaI GACGC 3 cut(s) 332, 625, 683
Hin1II CATG 4 cut(s) 92, 259, 296, 321
HincII GTYRAC 2 cut(s) 493, 615
HindII GTYRAC 2 cut(s) 493, 615
HindIII AAGCTT 1 cut(s) 280
HinfI GANTC 1 cut(s) 227
HpaI GTTAAC 1 cut(s) 493
HphI GGTGA 1 cut(s) 659
Hpy166II GTNNAC 6 cut(s) 253, 261, 493, 509, 586, 615
Hpy188I TCNGA 1 cut(s) 724
Hpy188III TCNNGA 4 cut(s) 34, 218, 269, 431
Hpy8I GTNNAC 6 cut(s) 253, 261, 493, 509, 586, 615
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 3 cut(s) 316, 347, 475
HpyCH4III ACNGT 3 cut(s) 153, 561, 612
HpyCH4V TGCA 1 cut(s) 586
HpyF10VI GCNNNNNNNGC 1 cut(s) 583
HpyF3I CTNAG 1 cut(s) 425
Hsp92II CATG 4 cut(s) 92, 259, 296, 321
KspAI GTTAAC 1 cut(s) 493
Kzo9I GATC 4 cut(s) 265, 364, 410, 484
LmnI GCTCC 2 cut(s) 6, 372
LpnPI CCDG 7 cut(s) 354, 381, 452, 479, 502, 603, 683
Lsp1109I GCAGC 1 cut(s) 314
MaeI CTAG 2 cut(s) 431, 501
MaeIII GTNAC 1 cut(s) 555
MalI GATC 4 cut(s) 267, 366, 412, 486
MbiI CCGCTC 1 cut(s) 9
MboI GATC 4 cut(s) 265, 364, 410, 484
MboII GAAGA 2 cut(s) 49, 52
MhlI GDGCHC 2 cut(s) 377, 588
MluCI AATT 4 cut(s) 182, 221, 531, 638
MlyI GAGTC 1 cut(s) 221
MnlI CCTC 5 cut(s) 185, 242, 319, 519, 535
MseI TTAA 3 cut(s) 81, 492, 716
MslI CAYNNNNRTG 1 cut(s) 591
MspR9I CCNGG 2 cut(s) 369, 467
MvaI CCWGG 2 cut(s) 369, 467
MwoI GCNNNNNNNGC 1 cut(s) 583
NdeII GATC 4 cut(s) 265, 364, 410, 484
NlaIII CATG 4 cut(s) 92, 259, 296, 321
NlaIV GGNNCC 3 cut(s) 64, 374, 688
NmuCI GTSAC 1 cut(s) 555
NspI RCATGY 2 cut(s) 259, 296
PceI AGGCCT 1 cut(s) 459
PciI ACATGT 2 cut(s) 255, 292
PkrI GCNGC 1 cut(s) 304
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PpuMI RGGWCCY 1 cut(s) 687
PscI ACATGT 2 cut(s) 255, 292
Psp5II RGGWCCY 1 cut(s) 687
Psp6I CCWGG 2 cut(s) 367, 465
PspGI CCWGG 2 cut(s) 367, 465
PspN4I GGNNCC 3 cut(s) 64, 374, 688
PspPI GGNCC 4 cut(s) 62, 311, 687, 710
PspPPI RGGWCCY 1 cut(s) 687
RsaI GTAC 2 cut(s) 508, 653
RsaNI GTAC 2 cut(s) 507, 652
RseI CAYNNNNRTG 1 cut(s) 591
SaqAI TTAA 3 cut(s) 81, 492, 716
SatI GCNGC 1 cut(s) 303
Sau3AI GATC 4 cut(s) 265, 364, 410, 484
Sau96I GGNCC 4 cut(s) 62, 311, 687, 710
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 2 cut(s) 369, 467
SduI GDGCHC 2 cut(s) 377, 588
SetI ASST 7 cut(s) 130, 147, 210, 284, 307, 471, 662
SinI GGWCC 2 cut(s) 687, 710
SmiMI CAYNNNNRTG 1 cut(s) 591
SmlI CTYRAG 2 cut(s) 203, 406
SmoI CTYRAG 2 cut(s) 203, 406
Sse9I AATT 4 cut(s) 182, 221, 531, 638
SseBI AGGCCT 1 cut(s) 459
SsiI CCGC 5 cut(s) 9, 56, 378, 402, 623
SspMI CTAG 2 cut(s) 431, 501
StuI AGGCCT 1 cut(s) 459
StyD4I CCNGG 2 cut(s) 367, 465
StyI CCWWGG 1 cut(s) 682
TaaI ACNGT 3 cut(s) 153, 561, 612
TaqI TCGA 6 cut(s) 123, 138, 177, 219, 276, 363
TasI AATT 4 cut(s) 182, 221, 531, 638
TatI WGTACW 1 cut(s) 651
Tru1I TTAA 3 cut(s) 81, 492, 716
Tru9I TTAA 3 cut(s) 81, 492, 716
TscAI CASTG 4 cut(s) 241, 573, 615, 637
TseFI GTSAC 1 cut(s) 555
TseI GCWGC 1 cut(s) 302
Tsp45I GTSAC 1 cut(s) 555
TspDTI ATGAA 1 cut(s) 651
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 4 cut(s) 241, 573, 615, 637
VneI GTGCAC 1 cut(s) 584
VpaK11BI GGWCC 2 cut(s) 687, 710
XagI CCTNNNNNAGG 1 cut(s) 464
XapI RAATTY 2 cut(s) 182, 638
XbaI TCTAGA 1 cut(s) 430
XceI RCATGY 2 cut(s) 259, 296
XmiI GTMKAC 1 cut(s) 260
XspI CTAG 2 cut(s) 431, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.