RLG00000008581

U3-containing 90S pre-ribosomal complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012458
Physical Location & Seq
Reverse (-)
32512294 .. 32517548
5255 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008581

Sequence Viewer

Length: 870 bp
ATGGGGAGCGGGAAAGAAAGGAACTCTAAGAACAAGGGTAAGCCGCTTGGCCCCAAAACAAGCAAACTCAAAGCCGACTCCCACTCCGGCATCAACAACAAGAACAAGAAGAAGAAGAAGCAGCAGCGGCGGCCCAACAAGAATACCCAAAATGAAAACCAAATCGGAACTCAAAACAGCGAAGACGGCAACAACAACAACAAGCTACAACCGGCATCGCTGTCGGCACAACTCAACTCGTTCCTCGACAAGTTTCAATCCGCCAATCGTCTCCAGCTTTCTTCTCTCGAATTGGACTCCCTCAATGATAAATGTATTGTGGACCTGTCTGGAAGCACGGATCAAGATGTCGAAAACTTGGGGAAACATGTGAAGGCAGCTTTTGGTCCATCGTGGAAAGCCGAACTATGTGATAAGCGTCTCTTGGAAGGAAAAGTTGATCCAGGAAGTCCAGCAGTTCTTATTATTAGTACATCTGCCTTGAGATCAATAGAACTTCTAAGGGGCTTTCGGTCGCTGACTAAAGAATGCCATCCAGTGAAGCTGTTCTCAAAGCATATGAAGGTTGACGAACAGGTCTCCTTGTTAAAGGACCGTGTTAACATCGCCAGTGGTACACCAAGCAGGATAAAGAAGCTAATTGACATTGAGGCATTGGGGCTTTCACGGTTATCAGTGATTGTGCTTGATGTGCACCCAGATGTAAAGGGCTATTCCCTGTTGACGCTTCCACAAGTCAGAGATGAATTTTGGGATTTGTACAAGACGTATTTTCACCAGCGATTACTCCAAGGTTCCTTGCGTCTTTCTCTCTATGGTCCATTAATAACTGATAATGAGTTCAAGGGAAAAAAGAAACCCACTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMS1

Protein Analysis

290

Amino Acids

32.25

Weight (kDa)

9.72

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMS1 PF14617 30 - 253 1.6e-30 U3-containing 90S pre-ribosomal complex subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43110 AT2G43110
fragaria_vesca FvH4_4g11840 FvH4_6g25320
malus_domestica MD04G1034000.v1.1 MD13G1276400.v1.1
prunus_persica Prupe.1G180600_v2.0.a1
pyrus_communis pycom04g02840
rosa_chinensis RchiOBHm_Chr4g0409281
rosa_laevigata RLG00000008581
rosa_multiflora Rmu_sc0002377.1_g000010
rosa_roxburghii Rroxscaffold_5G00353750 Rroxscaffold_7G00195060
rosa_rugosa Rorug04G0092000
rosa_samantha Rh4AG155000 Rh4BG152700 Rh4CG164000 Rh4DG148100
rosa_wichuraiana Rw4G012660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 575
AccBSI CCGCTC 1 cut(s) 9
AciI CCGC 5 cut(s) 9, 44, 127, 130, 261
AclWI GGATC 2 cut(s) 348, 434
AcsI RAATTY 1 cut(s) 746
AfaI GTAC 3 cut(s) 472, 616, 761
AflIII ACRYGT 1 cut(s) 367
AgsI TTSAA 2 cut(s) 257, 844
AjnI CCWGG 1 cut(s) 442
AluBI AGCT 5 cut(s) 205, 277, 380, 544, 637
AluI AGCT 5 cut(s) 205, 277, 380, 544, 637
Alw21I GWGCWC 1 cut(s) 696
Alw26I GTCTC 3 cut(s) 275, 425, 583
Alw44I GTGCAC 1 cut(s) 692
AlwI GGATC 2 cut(s) 348, 434
AoxI GGCC 2 cut(s) 49, 131
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 3 cut(s) 121, 124, 377
ApoI RAATTY 1 cut(s) 746
AseI ATTAAT 1 cut(s) 824
Asp700I GAANNNNTTC 1 cut(s) 545
AspS9I GGNCC 6 cut(s) 50, 132, 322, 386, 592, 818
AsuHPI GGTGA 1 cut(s) 767
AvaII GGWCC 4 cut(s) 322, 386, 592, 818
BaeGI GKGCMC 1 cut(s) 696
BbsI GAAGAC 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 696
BbvI GCAGC 3 cut(s) 133, 136, 389
BccI CCATC 2 cut(s) 397, 540
BceAI ACGGC 1 cut(s) 202
BcgI CGANNNNNNTGC 2 cut(s) 204, 238
BciT130I CCWGG 1 cut(s) 444
BcoDI GTCTC 3 cut(s) 275, 425, 583
BisI GCNGC 6 cut(s) 44, 122, 125, 128, 131, 378
BlsI GCNGC 6 cut(s) 45, 123, 126, 129, 132, 379
Bme1390I CCNGG 1 cut(s) 444
Bme18I GGWCC 4 cut(s) 322, 386, 592, 818
BmgT120I GGNCC 6 cut(s) 50, 132, 322, 386, 592, 818
BmiI GGNNCC 2 cut(s) 52, 796
BmrFI CCNGG 1 cut(s) 444
BmsI GCATC 2 cut(s) 99, 224
BpiI GAAGAC 1 cut(s) 189
BpmI CTGGAG 1 cut(s) 257
BpuEI CTTGAG 1 cut(s) 502
BsaI GGTCTC 1 cut(s) 583
BsaJI CCNNGG 1 cut(s) 790
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 2 cut(s) 536, 609
BseBI CCWGG 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 790
BseGI GGATG 1 cut(s) 532
BseNI ACTGG 2 cut(s) 536, 609
BseSI GKGCMC 1 cut(s) 696
BseXI GCAGC 3 cut(s) 133, 136, 389
Bsh1285I CGRYCG 1 cut(s) 515
BshFI GGCC 2 cut(s) 51, 133
BsiEI CGRYCG 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 696
BsiSI CCGG 2 cut(s) 87, 212
BsmAI GTCTC 3 cut(s) 275, 425, 583
BsmBI CGTCTC 2 cut(s) 275, 425
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 2 cut(s) 51, 133
Bso31I GGTCTC 1 cut(s) 583
Bsp1286I GDGCHC 1 cut(s) 696
Bsp1407I TGTACA 1 cut(s) 759
Bsp143I GATC 3 cut(s) 340, 439, 485
BspACI CCGC 5 cut(s) 9, 44, 127, 130, 261
BspANI GGCC 2 cut(s) 51, 133
BspLI GGNNCC 2 cut(s) 52, 796
BspPI GGATC 2 cut(s) 348, 434
BspTNI GGTCTC 1 cut(s) 583
BsrBI CCGCTC 1 cut(s) 9
BsrFI RCCGGY 1 cut(s) 211
BsrGI TGTACA 1 cut(s) 759
BsrI ACTGG 2 cut(s) 536, 609
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 1 cut(s) 790
BssMI GATC 3 cut(s) 340, 439, 485
BssT1I CCWWGG 1 cut(s) 790
Bst2UI CCWGG 1 cut(s) 444
Bst4CI ACNGT 2 cut(s) 596, 669
BstAUI TGTACA 1 cut(s) 759
BstDEI CTNAG 2 cut(s) 27, 500
BstF5I GGATG 1 cut(s) 532
BstKTI GATC 3 cut(s) 343, 442, 488
BstMAI GTCTC 3 cut(s) 275, 425, 583
BstMBI GATC 3 cut(s) 340, 439, 485
BstMCI CGRYCG 1 cut(s) 515
BstMWI GCNNNNNNNGC 4 cut(s) 127, 130, 186, 691
BstNI CCWGG 1 cut(s) 444
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 442
BstSLI GKGCMC 1 cut(s) 696
BstV1I GCAGC 3 cut(s) 133, 136, 389
BstV2I GAAGAC 1 cut(s) 189
BsuRI GGCC 2 cut(s) 51, 133
BtgZI GCGATG 2 cut(s) 201, 589
BtsCI GGATG 1 cut(s) 532
BtsIMutI CAGTG 4 cut(s) 543, 616, 681, 861
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 6 cut(s) 50, 132, 322, 386, 592, 818
CseI GACGC 3 cut(s) 407, 733, 791
Csp6I GTAC 3 cut(s) 471, 615, 760
CviAII CATG 1 cut(s) 368
CviQI GTAC 3 cut(s) 471, 615, 760
DdeI CTNAG 2 cut(s) 27, 500
DpnI GATC 3 cut(s) 342, 441, 487
DpnII GATC 3 cut(s) 340, 439, 485
DrdI GACNNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 1 cut(s) 575
EciI GGCGGA 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 790
Eco31I GGTCTC 1 cut(s) 583
Eco47I GGWCC 4 cut(s) 322, 386, 592, 818
EcoRII CCWGG 1 cut(s) 442
EcoT14I CCWWGG 1 cut(s) 790
ErhI CCWWGG 1 cut(s) 790
Esp3I CGTCTC 2 cut(s) 275, 425
FaeI CATG 1 cut(s) 371
FaiI YATR 5 cut(s) 369, 409, 558, 560, 816
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FatI CATG 1 cut(s) 367
FauI CCCGC 1 cut(s) 2
FauNDI CATATG 1 cut(s) 558
Fnu4HI GCNGC 6 cut(s) 44, 122, 125, 128, 131, 378
FokI GGATG 1 cut(s) 519
Fsp4HI GCNGC 6 cut(s) 44, 122, 125, 128, 131, 378
GluI GCNGC 6 cut(s) 44, 122, 125, 128, 131, 378
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 2 cut(s) 51, 133
HapII CCGG 2 cut(s) 87, 212
HgaI GACGC 3 cut(s) 407, 733, 791
Hin1II CATG 1 cut(s) 371
HincII GTYRAC 3 cut(s) 568, 601, 723
HindII GTYRAC 3 cut(s) 568, 601, 723
HinfI GANTC 2 cut(s) 77, 296
HpaI GTTAAC 1 cut(s) 601
HpaII CCGG 2 cut(s) 87, 212
HphI GGTGA 1 cut(s) 767
Hpy166II GTNNAC 6 cut(s) 322, 568, 601, 617, 694, 723
Hpy188I TCNGA 2 cut(s) 167, 740
Hpy188III TCNNGA 3 cut(s) 287, 330, 344
Hpy8I GTNNAC 6 cut(s) 322, 568, 601, 617, 694, 723
HpyAV CCTTC 3 cut(s) 367, 422, 556
HpyCH4III ACNGT 2 cut(s) 596, 669
HpyCH4IV ACGT 1 cut(s) 767
HpyCH4V TGCA 1 cut(s) 694
HpyF10VI GCNNNNNNNGC 4 cut(s) 127, 130, 186, 691
HpyF3I CTNAG 2 cut(s) 27, 500
HpySE526I ACGT 1 cut(s) 767
Hsp92II CATG 1 cut(s) 371
KspAI GTTAAC 1 cut(s) 601
Kzo9I GATC 3 cut(s) 340, 439, 485
LmnI GCTCC 1 cut(s) 6
Lsp1109I GCAGC 3 cut(s) 133, 136, 389
LweI GCATC 2 cut(s) 99, 224
MaeII ACGT 1 cut(s) 767
MalI GATC 3 cut(s) 342, 441, 487
MbiI CCGCTC 1 cut(s) 9
MboI GATC 3 cut(s) 340, 439, 485
MboII GAAGA 5 cut(s) 121, 124, 127, 194, 273
MhlI GDGCHC 1 cut(s) 696
MluCI AATT 3 cut(s) 290, 639, 746
MlyI GAGTC 2 cut(s) 71, 290
MnlI CCTC 3 cut(s) 254, 311, 643
MroXI GAANNNNTTC 1 cut(s) 545
MseI TTAA 3 cut(s) 587, 600, 824
MslI CAYNNNNRTG 1 cut(s) 699
MspA1I CMGCKG 1 cut(s) 127
MspI CCGG 2 cut(s) 87, 212
MspR9I CCNGG 1 cut(s) 444
Mva1269I GAATGC 1 cut(s) 533
MvaI CCWGG 1 cut(s) 444
MwoI GCNNNNNNNGC 4 cut(s) 127, 130, 186, 691
NdeI CATATG 1 cut(s) 558
NdeII GATC 3 cut(s) 340, 439, 485
NlaIII CATG 1 cut(s) 371
NlaIV GGNNCC 2 cut(s) 52, 796
NspI RCATGY 1 cut(s) 371
PciI ACATGT 1 cut(s) 367
PctI GAATGC 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 545
PfoI TCCNGGA 1 cut(s) 442
PkrI GCNGC 6 cut(s) 45, 123, 126, 129, 132, 379
PleI GAGTC 2 cut(s) 71, 290
PpsI GAGTC 2 cut(s) 71, 290
PscI ACATGT 1 cut(s) 367
PshBI ATTAAT 1 cut(s) 824
Psp6I CCWGG 1 cut(s) 442
PspGI CCWGG 1 cut(s) 442
PspN4I GGNNCC 2 cut(s) 52, 796
PspPI GGNCC 6 cut(s) 50, 132, 322, 386, 592, 818
RsaI GTAC 3 cut(s) 472, 616, 761
RsaNI GTAC 3 cut(s) 471, 615, 760
RseI CAYNNNNRTG 1 cut(s) 699
SaqAI TTAA 3 cut(s) 587, 600, 824
SatI GCNGC 6 cut(s) 44, 122, 125, 128, 131, 378
Sau3AI GATC 3 cut(s) 340, 439, 485
Sau96I GGNCC 6 cut(s) 50, 132, 322, 386, 592, 818
SchI GAGTC 2 cut(s) 71, 290
ScrFI CCNGG 1 cut(s) 444
SduI GDGCHC 1 cut(s) 696
SfaNI GCATC 2 cut(s) 99, 224
SinI GGWCC 4 cut(s) 322, 386, 592, 818
SmiMI CAYNNNNRTG 1 cut(s) 699
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 3 cut(s) 290, 639, 746
SsiI CCGC 5 cut(s) 9, 44, 127, 130, 261
StyD4I CCNGG 1 cut(s) 442
StyI CCWWGG 1 cut(s) 790
TaaI ACNGT 2 cut(s) 596, 669
TaiI ACGT 1 cut(s) 770
TaqI TCGA 3 cut(s) 246, 288, 351
TaqII GACCGA 1 cut(s) 501
TasI AATT 3 cut(s) 290, 639, 746
TatI WGTACW 2 cut(s) 470, 759
TauI GCSGC 3 cut(s) 46, 130, 133
Tru1I TTAA 3 cut(s) 587, 600, 824
Tru9I TTAA 3 cut(s) 587, 600, 824
TscAI CASTG 4 cut(s) 543, 616, 681, 868
TseI GCWGC 3 cut(s) 121, 124, 377
TspDTI ATGAA 3 cut(s) 168, 575, 759
TspGWI ACGGA 1 cut(s) 353
TspRI CASTG 4 cut(s) 543, 616, 681, 868
VneI GTGCAC 1 cut(s) 692
VpaK11BI GGWCC 4 cut(s) 322, 386, 592, 818
VspI ATTAAT 1 cut(s) 824
XapI RAATTY 1 cut(s) 746
XceI RCATGY 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.