Rmu_sc0002377.1_g000010

U3-containing 90S pre-ribosomal complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002377.1
OG0012458
Physical Location & Seq
Reverse (-)
73281 .. 78144
4864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002377.1_g000010.1.cds

Sequence Viewer

Length: 855 bp
atggggagcgggaaagaaaggaactctaagaacaagggtaagccgcttggccccaaaacaaccaaactcaaagccgactcccactccggcatcaacaacaagaacaagaacaagaagcagcagcggcccaacaagaatacccaaaatgaaaaccaaatcggaactcaaaacagcgaagacagcaagctacaaccggcgtcgccgtcggcacaactcaactcgttcctcgacaagtttcaatccgccaatcgtctccagctttcttctctcgaattggactccctcactgataagtgtattgtggacctgtctggaagcacggatcaggatgtcgaaaacttggggaaacatgtgaaggcagcttttggtccatcgtggaaagcccaactatgtgataagcgtctcttggaaggaaaagttgatccaggaagtccagcagttcttattattagtacatctgccttgagatcaatagaacttctaaggggctttcggtcgctgactaaagaatgccatccagtgaagctgttctcaaagcatatgaaggttgacgaacaggtctccttgttaaaggaccgtgttaacatcgctagtggtacaccaagcaggataaagaagctaattgatattgaggcattgggactttcacggttatcagtgattgtgcttgatgtgcacccagatgtaaagggctattccctgttgacgcttccacaagtcagagatgaattttgggacttgtacaagacctattttcaccagcgattactccaaggttccttgcgtctctctctctatggtccattaatatctgataacgagttcaagggaaaaaagaagagacccaatgaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMS1

Protein Analysis

284

Amino Acids

31.67

Weight (kDa)

9.7

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43110 AT2G43110
fragaria_vesca FvH4_4g11840 FvH4_6g25320
malus_domestica MD04G1034000.v1.1 MD13G1276400.v1.1
prunus_persica Prupe.1G180600_v2.0.a1
pyrus_communis pycom04g02840
rosa_chinensis RchiOBHm_Chr4g0409281
rosa_laevigata RLG00000008581
rosa_multiflora Rmu_sc0002377.1_g000010
rosa_roxburghii Rroxscaffold_5G00353750 Rroxscaffold_7G00195060
rosa_rugosa Rorug04G0092000
rosa_samantha Rh4AG155000 Rh4BG152700 Rh4CG164000 Rh4DG148100
rosa_wichuraiana Rw4G012660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 557
AccBSI CCGCTC 1 cut(s) 9
AciI CCGC 4 cut(s) 9, 44, 124, 243
AclWI GGATC 2 cut(s) 330, 416
AcsI RAATTY 1 cut(s) 728
AcyI GRCGYC 1 cut(s) 197
AfaI GTAC 3 cut(s) 454, 598, 743
AflIII ACRYGT 1 cut(s) 349
AgsI TTSAA 2 cut(s) 239, 826
AjnI CCWGG 1 cut(s) 424
AluBI AGCT 5 cut(s) 187, 259, 362, 526, 619
AluI AGCT 5 cut(s) 187, 259, 362, 526, 619
Alw21I GWGCWC 1 cut(s) 678
Alw26I GTCTC 5 cut(s) 257, 407, 565, 791, 835
Alw44I GTGCAC 1 cut(s) 674
AlwI GGATC 2 cut(s) 330, 416
AoxI GGCC 2 cut(s) 49, 125
ApaLI GTGCAC 1 cut(s) 674
ApeKI GCWGC 3 cut(s) 118, 121, 359
ApoI RAATTY 1 cut(s) 728
AseI ATTAAT 1 cut(s) 806
Asp700I GAANNNNTTC 1 cut(s) 527
AspS9I GGNCC 6 cut(s) 50, 126, 304, 368, 574, 800
AsuHPI GGTGA 1 cut(s) 749
AvaII GGWCC 4 cut(s) 304, 368, 574, 800
BaeGI GKGCMC 1 cut(s) 678
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 678
BbvI GCAGC 3 cut(s) 130, 133, 371
BccI CCATC 2 cut(s) 379, 522
BceAI ACGGC 1 cut(s) 187
BciT130I CCWGG 1 cut(s) 426
BcoDI GTCTC 5 cut(s) 257, 407, 565, 791, 835
BfaI CTAG 1 cut(s) 591
BisI GCNGC 5 cut(s) 44, 119, 122, 125, 360
BlsI GCNGC 5 cut(s) 45, 120, 123, 126, 361
Bme1390I CCNGG 1 cut(s) 426
Bme18I GGWCC 4 cut(s) 304, 368, 574, 800
BmgT120I GGNCC 6 cut(s) 50, 126, 304, 368, 574, 800
BmiI GGNNCC 2 cut(s) 52, 778
BmrFI CCNGG 1 cut(s) 426
BmsI GCATC 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 183
BpmI CTGGAG 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 484
BsaHI GRCGYC 1 cut(s) 197
BsaI GGTCTC 2 cut(s) 565, 835
BsaJI CCNNGG 1 cut(s) 772
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 518
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 772
BseGI GGATG 2 cut(s) 334, 514
BseNI ACTGG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 678
BseXI GCAGC 3 cut(s) 130, 133, 371
Bsh1285I CGRYCG 1 cut(s) 497
BshFI GGCC 2 cut(s) 51, 127
BsiEI CGRYCG 1 cut(s) 497
BsiHKAI GWGCWC 1 cut(s) 678
BsiSI CCGG 2 cut(s) 87, 194
BslFI GGGAC 2 cut(s) 654, 749
BsmAI GTCTC 5 cut(s) 257, 407, 565, 791, 835
BsmBI CGTCTC 3 cut(s) 257, 407, 791
BsmFI GGGAC 2 cut(s) 654, 749
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 2 cut(s) 51, 127
Bso31I GGTCTC 2 cut(s) 565, 835
Bsp1286I GDGCHC 1 cut(s) 678
Bsp1407I TGTACA 1 cut(s) 741
Bsp143I GATC 3 cut(s) 322, 421, 467
BspACI CCGC 4 cut(s) 9, 44, 124, 243
BspANI GGCC 2 cut(s) 51, 127
BspLI GGNNCC 2 cut(s) 52, 778
BspPI GGATC 2 cut(s) 330, 416
BspTNI GGTCTC 2 cut(s) 565, 835
BsrBI CCGCTC 1 cut(s) 9
BsrFI RCCGGY 1 cut(s) 193
BsrGI TGTACA 1 cut(s) 741
BsrI ACTGG 1 cut(s) 518
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 772
BssMI GATC 3 cut(s) 322, 421, 467
BssNI GRCGYC 1 cut(s) 197
BssT1I CCWWGG 1 cut(s) 772
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 2 cut(s) 578, 651
Bst6I CTCTTC 1 cut(s) 833
BstACI GRCGYC 1 cut(s) 197
BstAUI TGTACA 1 cut(s) 741
BstC8I GCNNGC 1 cut(s) 185
BstDEI CTNAG 2 cut(s) 27, 482
BstF5I GGATG 2 cut(s) 334, 514
BstKTI GATC 3 cut(s) 325, 424, 470
BstMAI GTCTC 5 cut(s) 257, 407, 565, 791, 835
BstMBI GATC 3 cut(s) 322, 421, 467
BstMCI CGRYCG 1 cut(s) 497
BstMWI GCNNNNNNNGC 3 cut(s) 124, 180, 673
BstNI CCWGG 1 cut(s) 426
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 424
BstSLI GKGCMC 1 cut(s) 678
BstV1I GCAGC 3 cut(s) 130, 133, 371
BstV2I GAAGAC 1 cut(s) 183
BsuRI GGCC 2 cut(s) 51, 127
BtgZI GCGATG 1 cut(s) 571
BtsCI GGATG 2 cut(s) 334, 514
BtsIMutI CAGTG 3 cut(s) 285, 525, 663
Cac8I GCNNGC 1 cut(s) 185
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 6 cut(s) 50, 126, 304, 368, 574, 800
CseI GACGC 4 cut(s) 186, 389, 715, 773
Csp6I GTAC 3 cut(s) 453, 597, 742
CviAII CATG 1 cut(s) 350
CviQI GTAC 3 cut(s) 453, 597, 742
DdeI CTNAG 2 cut(s) 27, 482
DpnI GATC 3 cut(s) 324, 423, 469
DpnII GATC 3 cut(s) 322, 421, 467
DrdI GACNNNNNNGTC 1 cut(s) 557
DseDI GACNNNNNNGTC 1 cut(s) 557
Eam1104I CTCTTC 1 cut(s) 833
EarI CTCTTC 1 cut(s) 833
EciI GGCGGA 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 772
Eco31I GGTCTC 2 cut(s) 565, 835
Eco47I GGWCC 4 cut(s) 304, 368, 574, 800
EcoRII CCWGG 1 cut(s) 424
EcoT14I CCWWGG 1 cut(s) 772
ErhI CCWWGG 1 cut(s) 772
Esp3I CGTCTC 3 cut(s) 257, 407, 791
FaeI CATG 1 cut(s) 353
FaiI YATR 5 cut(s) 351, 391, 540, 542, 798
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FaqI GGGAC 2 cut(s) 654, 749
FatI CATG 1 cut(s) 349
FauI CCCGC 1 cut(s) 2
FauNDI CATATG 1 cut(s) 540
Fnu4HI GCNGC 5 cut(s) 44, 119, 122, 125, 360
FokI GGATG 2 cut(s) 341, 501
Fsp4HI GCNGC 5 cut(s) 44, 119, 122, 125, 360
FspBI CTAG 1 cut(s) 591
GluI GCNGC 5 cut(s) 44, 119, 122, 125, 360
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 2 cut(s) 51, 127
HapII CCGG 2 cut(s) 87, 194
HgaI GACGC 4 cut(s) 186, 389, 715, 773
Hin1I GRCGYC 1 cut(s) 197
Hin1II CATG 1 cut(s) 353
HincII GTYRAC 3 cut(s) 550, 583, 705
HindII GTYRAC 3 cut(s) 550, 583, 705
HinfI GANTC 2 cut(s) 77, 278
HpaI GTTAAC 1 cut(s) 583
HpaII CCGG 2 cut(s) 87, 194
HphI GGTGA 1 cut(s) 749
Hpy166II GTNNAC 6 cut(s) 304, 550, 583, 599, 676, 705
Hpy188I TCNGA 3 cut(s) 161, 722, 814
Hpy188III TCNNGA 3 cut(s) 269, 312, 326
Hpy8I GTNNAC 6 cut(s) 304, 550, 583, 599, 676, 705
Hpy99I CGWCG 2 cut(s) 202, 208
HpyAV CCTTC 3 cut(s) 349, 404, 538
HpyCH4III ACNGT 2 cut(s) 578, 651
HpyCH4V TGCA 1 cut(s) 676
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 180, 673
HpyF3I CTNAG 2 cut(s) 27, 482
Hsp92I GRCGYC 1 cut(s) 197
Hsp92II CATG 1 cut(s) 353
KspAI GTTAAC 1 cut(s) 583
Kzo9I GATC 3 cut(s) 322, 421, 467
LmnI GCTCC 1 cut(s) 6
Lsp1109I GCAGC 3 cut(s) 130, 133, 371
LweI GCATC 1 cut(s) 99
MaeI CTAG 1 cut(s) 591
MalI GATC 3 cut(s) 324, 423, 469
MbiI CCGCTC 1 cut(s) 9
MboI GATC 3 cut(s) 322, 421, 467
MboII GAAGA 3 cut(s) 188, 255, 850
MhlI GDGCHC 1 cut(s) 678
MluCI AATT 3 cut(s) 272, 621, 728
MlyI GAGTC 2 cut(s) 71, 272
MnlI CCTC 3 cut(s) 236, 293, 625
MroXI GAANNNNTTC 1 cut(s) 527
MseI TTAA 3 cut(s) 569, 582, 806
MslI CAYNNNNRTG 1 cut(s) 681
MspA1I CMGCKG 1 cut(s) 124
MspI CCGG 2 cut(s) 87, 194
MspR9I CCNGG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 515
MvaI CCWGG 1 cut(s) 426
MwoI GCNNNNNNNGC 3 cut(s) 124, 180, 673
NdeI CATATG 1 cut(s) 540
NdeII GATC 3 cut(s) 322, 421, 467
NlaIII CATG 1 cut(s) 353
NlaIV GGNNCC 2 cut(s) 52, 778
NspI RCATGY 1 cut(s) 353
PciI ACATGT 1 cut(s) 349
PctI GAATGC 1 cut(s) 515
PdmI GAANNNNTTC 1 cut(s) 527
PfoI TCCNGGA 1 cut(s) 424
PkrI GCNGC 5 cut(s) 45, 120, 123, 126, 361
PleI GAGTC 2 cut(s) 71, 272
PpsI GAGTC 2 cut(s) 71, 272
PscI ACATGT 1 cut(s) 349
PshBI ATTAAT 1 cut(s) 806
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 2 cut(s) 52, 778
PspPI GGNCC 6 cut(s) 50, 126, 304, 368, 574, 800
RsaI GTAC 3 cut(s) 454, 598, 743
RsaNI GTAC 3 cut(s) 453, 597, 742
RseI CAYNNNNRTG 1 cut(s) 681
SaqAI TTAA 3 cut(s) 569, 582, 806
SatI GCNGC 5 cut(s) 44, 119, 122, 125, 360
Sau3AI GATC 3 cut(s) 322, 421, 467
Sau96I GGNCC 6 cut(s) 50, 126, 304, 368, 574, 800
SchI GAGTC 2 cut(s) 71, 272
ScrFI CCNGG 1 cut(s) 426
SduI GDGCHC 1 cut(s) 678
SfaNI GCATC 1 cut(s) 99
SinI GGWCC 4 cut(s) 304, 368, 574, 800
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 1 cut(s) 463
SmoI CTYRAG 1 cut(s) 463
Sse9I AATT 3 cut(s) 272, 621, 728
SsiI CCGC 4 cut(s) 9, 44, 124, 243
SspMI CTAG 1 cut(s) 591
StyD4I CCNGG 1 cut(s) 424
StyI CCWWGG 1 cut(s) 772
TaaI ACNGT 2 cut(s) 578, 651
TaqI TCGA 3 cut(s) 228, 270, 333
TaqII GACCGA 1 cut(s) 483
TasI AATT 3 cut(s) 272, 621, 728
TatI WGTACW 2 cut(s) 452, 741
TauI GCSGC 2 cut(s) 46, 127
Tru1I TTAA 3 cut(s) 569, 582, 806
Tru9I TTAA 3 cut(s) 569, 582, 806
TscAI CASTG 3 cut(s) 292, 525, 663
TseI GCWGC 3 cut(s) 118, 121, 359
TspDTI ATGAA 3 cut(s) 162, 557, 741
TspGWI ACGGA 1 cut(s) 335
TspRI CASTG 3 cut(s) 292, 525, 663
VneI GTGCAC 1 cut(s) 674
VpaK11BI GGWCC 4 cut(s) 304, 368, 574, 800
VspI ATTAAT 1 cut(s) 806
XapI RAATTY 1 cut(s) 728
XceI RCATGY 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 527
XspI CTAG 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.