FvH4_6g27150

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009472
Physical Location & Seq
Forward (+)
20865421 .. 20869418
3998 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27150.t2

Sequence Viewer

Length: 1761 bp
ATGGCGGCTGCTACTTCCTCTTTCTCTCCAGCCCTCACCGCTCCACCTTCCTCATCCTCCGCCGCCGCCTCCAAAACCTACCCGGCTCCCTCCTCTCTCGGCTTCTCCTCCGCCCTCTCCAAGTCTCTCCACCCTCTCAGGGCCAGAGCCACTCGCGCTGCTGCTAATGGCTCCGCTCTCGGAGCTCGTATGGTCTCGGTGCCGGCGATTAAGACTCCTCTTTCGCTTGATTTCGAGACCTCTGTTTTCAAGAAGGAGAAGATCAACCTCGCTGGTCACGATGAGTATATCGTGAGAGGAGGAAGGGACTTATTCCATCTGCTGCCTGATGCCTTTAAAGGTATCAAGCAGATTGGTGTTATCGGTTGGGGTTCTCAGGGACCTGCTCAAGCTCAGAACTTAAGAGATTCTCTTGCTGAAGCGAAGTCTGATATTGTTGTCAAGATTGGGCTTAGGAAGGGATCCCGTTCATTTAATGAAGCTCGGGCAGTTGGTTTCACTGAGGAAAGTGGGACTTTGGGTGATATATGGGAAACAGTCTCAAGCAGTGATCTTGTGTTGCTGCTAATTTCTGATTCTGCACAGGCAGATAATTATGAGAAAATATTTTCGCACATGAAACCAAATAGCATACTTGGTCTTTCCCACGGTTTCCTTCTGGGGCATTTGCAGTCAGTGGGACTTGACTTTCCCAAGAACATCAGTGTAATTGCTGTATGCCCAAAGGGAATGGGTCCATCAGTAAGGAGACTTTATGTCCAAGGCAAAGAAATAAATGGTGCGGGAATTAATTCAAGTTTTGCAGTCCACCAGGATGTTGATGGGAGAGCCACAGATGTGGCCCTGGGCTGGTCCGTTGCCCTTGGTTCTCCTTTCTCGTTTGTCACTACACTAGAGCAGGAATACAAAAGTGACATCTTTGGCGAGAGAGGCATATTACTTGGTGCTGTTCATGGAATTGTAGAATCCCTTTTCAGAAGATACACTGACAGTGGAATGAGTGAAGATTCTGCTTATAAAAACACTGTTGAATGTGTAACTGGAATCGTGTCAAGGACCATTTCAACCAAGGGCATGTTAGAAATATACAACTCTTTATCTGAAGCAGACAAGAAGGAATTCGAGGCTGCATACAGTGCCTCGTATTATCCCTGCATGGACATCCTCTATGAATGCTATGAGGATGTGGCAAGTGGCAGTGAAATCCGCAGTGTAGTGTTGGCTGGGCGCCGATTTTATGAGAAGGATGGTCTTCCTGCCTTCCCTATGGGGAAGATTGATCAGACGCGCATGTGGAAAGTTGGTGAGCGTGTCCGCGCGACACGCCCTGCTGGTGATCTAGGCCCACTACACCCGTTTACAGCTGGTGTTTTTGTGGCCTTGATGATGGCACAGATTGAAGTGTTGAGGAAGAAGGGACACTCTTACTCAGAGATAATCAATGAAAGTGTCATTGAGTCGGTGGATTCCTTGAATCCTTTTATGCATGCTAGGGGAGTATCTTTCATGGTAGATAATTGCTCAACAACAGCCCGGTTGGGATCAAGGAAATGGGCTCCAAGATTTGATTATATTCTAACTCAGCAAGCGTTGGTTGCCGTGGACAACGGTACACCCATCAACCAGGACCTCATTAGCAATTTCCTGTCAGATCCAGTGCATGGAGCCATTGAAGTATGCGCTCAACTTAGACCCACAGTTGATATTTCAGTGCCTGCTGACGCAGACTTTGTGCGACCAGAGTTGCGCTCTAGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

587

Amino Acids

63.21

Weight (kDa)

6.34

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KARI_N PF07991 31 - 205 7e-31 Acetohydroxy acid isomeroreductase, NADPH-binding domain
KARI_C PF01450 214 - 358 5.2e-34 Ketol-acid reductoisomerase, C-terminal domain
KARI_C PF01450 370 - 439 3.6e-07 Ketol-acid reductoisomerase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
Acc36I ACCTGC 1 cut(s) 391
AccB1I GGYRCC 2 cut(s) 199, 1227
AccB7I CCANNNNNTGG 1 cut(s) 1661
AccBSI CCGCTC 2 cut(s) 41, 176
AccII CGCG 4 cut(s) 156, 1288, 1317, 1319
AclWI GGATC 4 cut(s) 456, 469, 1549, 1646
AcsI RAATTY 1 cut(s) 1118
AcuI CTGAAG 2 cut(s) 438, 1122
AcyI GRCGYC 1 cut(s) 1228
AfaI GTAC 1 cut(s) 1612
AfiI CCNNNNNNNGG 3 cut(s) 139, 849, 1661
AflII CTTAAG 1 cut(s) 400
AgsI TTSAA 7 cut(s) 250, 795, 1031, 1065, 1400, 1474, 1673
AhdI GACNNNNNGTC 1 cut(s) 755
AjnI CCWGG 3 cut(s) 810, 843, 1623
AleI CACNNNNGTG 1 cut(s) 836
AluBI AGCT 4 cut(s) 185, 392, 482, 1364
AluI AGCT 4 cut(s) 185, 392, 482, 1364
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 5 cut(s) 129, 199, 230, 544, 742
AlwI GGATC 4 cut(s) 456, 469, 1549, 1646
AlwNI CAGNNNCTG 1 cut(s) 1715
Ama87I CYCGRG 1 cut(s) 483
AoxI GGCC 4 cut(s) 141, 840, 1342, 1377
ApeKI GCWGC 6 cut(s) 8, 158, 161, 322, 562, 1127
ApoI RAATTY 1 cut(s) 1118
ArsI GACNNNNNNTTYG 4 cut(s) 205, 237, 1712, 1744
AseI ATTAAT 1 cut(s) 789
Asp700I GAANNNNTTC 2 cut(s) 790, 1118
AspLEI GCGC 6 cut(s) 158, 1230, 1290, 1319, 1682, 1749
AspS9I GGNCC 8 cut(s) 141, 380, 734, 841, 852, 1056, 1343, 1627
AsuC2I CCSGG 2 cut(s) 83, 1534
AsuHPI GGTGA 4 cut(s) 28, 533, 1316, 1346
AvaI CYCGRG 1 cut(s) 483
AvaII GGWCC 5 cut(s) 380, 734, 852, 1056, 1627
BamHI GGATCC 1 cut(s) 461
BanI GGYRCC 2 cut(s) 199, 1227
BanII GRGCYC 2 cut(s) 187, 1558
BbsI GAAGAC 1 cut(s) 1244
Bbv12I GWGCWC 1 cut(s) 187
BbvI GCAGC 5 cut(s) 145, 148, 309, 549, 1114
BccI CCATC 6 cut(s) 324, 745, 815, 1241, 1381, 1625
BceAI ACGGC 1 cut(s) 1583
BciT130I CCWGG 3 cut(s) 812, 845, 1625
BclI TGATCA 1 cut(s) 1279
BcnI CCSGG 2 cut(s) 83, 1534
BcoDI GTCTC 5 cut(s) 129, 199, 230, 544, 742
BfaI CTAG 4 cut(s) 893, 1340, 1491, 1752
BfoI RGCGCY 1 cut(s) 1231
BfrI CTTAAG 1 cut(s) 400
BfuAI ACCTGC 1 cut(s) 391
BisI GCNGC 9 cut(s) 6, 9, 63, 66, 159, 162, 323, 563, 1128
BlsI GCNGC 9 cut(s) 7, 10, 64, 67, 160, 163, 324, 564, 1129
Bme1390I CCNGG 5 cut(s) 83, 812, 845, 1534, 1625
Bme18I GGWCC 5 cut(s) 380, 734, 852, 1056, 1627
BmeRI GACNNNNNGTC 1 cut(s) 755
BmeT110I CYCGRG 1 cut(s) 483
BmgT120I GGNCC 8 cut(s) 141, 380, 734, 841, 852, 1056, 1343, 1627
BmiI GGNNCC 9 cut(s) 87, 172, 201, 381, 463, 735, 1229, 1557, 1666
BmrFI CCNGG 5 cut(s) 83, 812, 845, 1534, 1625
BmsI GCATC 1 cut(s) 319
BpiI GAAGAC 1 cut(s) 1244
BplI GAGNNNNNCTC 1 cut(s) 1733
BpmI CTGGAG 1 cut(s) 12
Bpu10I CCTNAGC 1 cut(s) 452
BpuEI CTTGAG 2 cut(s) 372, 526
BpuMI CCSGG 2 cut(s) 83, 1534
BsaHI GRCGYC 1 cut(s) 1228
BsaI GGTCTC 2 cut(s) 199, 230
BsaJI CCNNGG 7 cut(s) 646, 760, 843, 844, 862, 1068, 1599
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 849, 1661
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 1045, 1655
BseBI CCWGG 3 cut(s) 812, 845, 1625
BseDI CCNNGG 7 cut(s) 646, 760, 843, 844, 862, 1068, 1599
BseGI GGATG 5 cut(s) 53, 820, 1161, 1189, 1252
BseLI CCNNNNNNNGG 3 cut(s) 139, 849, 1661
BseMII CTCAG 6 cut(s) 151, 389, 407, 492, 1443, 1595
BseNI ACTGG 2 cut(s) 1045, 1655
BseRI GAGGAG 4 cut(s) 82, 97, 207, 312
BseXI GCAGC 5 cut(s) 145, 148, 309, 549, 1114
BseYI CCCAGC 1 cut(s) 1223
BsgI GTGCAG 1 cut(s) 564
Bsh1236I CGCG 4 cut(s) 156, 1288, 1317, 1319
BshFI GGCC 4 cut(s) 143, 842, 1344, 1379
BshNI GGYRCC 2 cut(s) 199, 1227
BsiHKAI GWGCWC 1 cut(s) 187
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 3 cut(s) 83, 203, 1534
BslFI GGGAC 5 cut(s) 320, 393, 526, 693, 1431
BslI CCNNNNNNNGG 3 cut(s) 139, 849, 1661
BsmAI GTCTC 5 cut(s) 129, 199, 230, 544, 742
BsmFI GGGAC 5 cut(s) 320, 393, 526, 693, 1431
BsmI GAATGC 1 cut(s) 1178
BsnI GGCC 4 cut(s) 143, 842, 1344, 1379
Bso31I GGTCTC 2 cut(s) 199, 230
BsoBI CYCGRG 1 cut(s) 483
Bsp1286I GDGCHC 2 cut(s) 187, 1558
Bsp143I GATC 7 cut(s) 261, 461, 550, 1279, 1336, 1541, 1651
BspANI GGCC 4 cut(s) 143, 842, 1344, 1379
BspCNI CTCAG 6 cut(s) 150, 388, 406, 493, 1442, 1594
BspFNI CGCG 4 cut(s) 156, 1288, 1317, 1319
BspLI GGNNCC 9 cut(s) 87, 172, 201, 381, 463, 735, 1229, 1557, 1666
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 4 cut(s) 456, 469, 1549, 1646
BspT107I GGYRCC 2 cut(s) 199, 1227
BspTI CTTAAG 1 cut(s) 400
BspTNI GGTCTC 2 cut(s) 199, 230
BsrBI CCGCTC 2 cut(s) 41, 176
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 2 cut(s) 1045, 1655
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 7 cut(s) 646, 760, 843, 844, 862, 1068, 1599
BssMI GATC 7 cut(s) 261, 461, 550, 1279, 1336, 1541, 1651
BssNI GRCGYC 1 cut(s) 1228
BssT1I CCWWGG 3 cut(s) 760, 862, 1068
Bst2UI CCWGG 3 cut(s) 812, 845, 1625
Bst4CI ACNGT 7 cut(s) 538, 650, 992, 1027, 1136, 1610, 1699
BstACI GRCGYC 1 cut(s) 1228
BstAFI CTTAAG 1 cut(s) 400
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstC8I GCNNGC 4 cut(s) 204, 1488, 1587, 1716
BstDEI CTNAG 8 cut(s) 137, 375, 393, 452, 501, 1429, 1581, 1688
BstDSI CCRYGG 2 cut(s) 646, 1599
BstF5I GGATG 5 cut(s) 53, 820, 1161, 1189, 1252
BstFNI CGCG 4 cut(s) 156, 1288, 1317, 1319
BstH2I RGCGCY 1 cut(s) 1231
BstHHI GCGC 6 cut(s) 158, 1230, 1290, 1319, 1682, 1749
BstKTI GATC 7 cut(s) 264, 464, 553, 1282, 1339, 1544, 1654
BstMAI GTCTC 5 cut(s) 129, 199, 230, 544, 742
BstMBI GATC 7 cut(s) 261, 461, 550, 1279, 1336, 1541, 1651
BstMWI GCNNNNNNNGC 7 cut(s) 38, 155, 182, 930, 1136, 1323, 1595
BstNI CCWGG 3 cut(s) 812, 845, 1625
BstNSI RCATGY 3 cut(s) 1078, 1294, 1490
BstSCI CCNGG 5 cut(s) 81, 810, 843, 1532, 1623
BstUI CGCG 4 cut(s) 156, 1288, 1317, 1319
BstV1I GCAGC 5 cut(s) 145, 148, 309, 549, 1114
BstV2I GAAGAC 1 cut(s) 1244
BstX2I RGATCY 2 cut(s) 461, 1651
BstXI CCANNNNNNTGG 1 cut(s) 838
BstYI RGATCY 2 cut(s) 461, 1651
BsuRI GGCC 4 cut(s) 143, 842, 1344, 1379
BtgI CCRYGG 2 cut(s) 646, 1599
BtsCI GGATG 5 cut(s) 53, 820, 1161, 1189, 1252
BtsI GCAGTG 3 cut(s) 553, 1204, 1216
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 4 cut(s) 204, 1488, 1587, 1716
CaiI CAGNNNCTG 1 cut(s) 1715
CfoI GCGC 6 cut(s) 158, 1230, 1290, 1319, 1682, 1749
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 8 cut(s) 141, 380, 734, 841, 852, 1056, 1343, 1627
CseI GACGC 2 cut(s) 1294, 1730
Csp6I GTAC 1 cut(s) 1611
CviAII CATG 8 cut(s) 616, 953, 1075, 1156, 1291, 1487, 1507, 1661
CviQI GTAC 1 cut(s) 1611
DdeI CTNAG 8 cut(s) 137, 375, 393, 452, 501, 1429, 1581, 1688
DinI GGCGCC 1 cut(s) 1229
DpnI GATC 7 cut(s) 263, 463, 552, 1281, 1338, 1543, 1653
DpnII GATC 7 cut(s) 261, 461, 550, 1279, 1336, 1541, 1651
DraI TTTAAA 1 cut(s) 337
DriI GACNNNNNGTC 1 cut(s) 755
Eam1105I GACNNNNNGTC 1 cut(s) 755
EciI GGCGGA 2 cut(s) 49, 100
Ecl136II GAGCTC 1 cut(s) 185
Eco130I CCWWGG 3 cut(s) 760, 862, 1068
Eco24I GRGCYC 2 cut(s) 187, 1558
Eco31I GGTCTC 2 cut(s) 199, 230
Eco47I GGWCC 5 cut(s) 380, 734, 852, 1056, 1627
Eco53kI GAGCTC 1 cut(s) 185
Eco57I CTGAAG 2 cut(s) 438, 1122
Eco88I CYCGRG 1 cut(s) 483
EcoICRI GAGCTC 1 cut(s) 185
EcoO109I RGGNCCY 2 cut(s) 380, 1627
EcoRI GAATTC 1 cut(s) 1118
EcoRII CCWGG 3 cut(s) 810, 843, 1623
EcoT14I CCWWGG 3 cut(s) 760, 862, 1068
EcoT22I ATGCAT 1 cut(s) 1488
EcoT38I GRGCYC 2 cut(s) 187, 1558
EgeI GGCGCC 1 cut(s) 1229
EheI GGCGCC 1 cut(s) 1229
ErhI CCWWGG 3 cut(s) 760, 862, 1068
FaeI CATG 8 cut(s) 619, 956, 1078, 1159, 1294, 1490, 1510, 1664
FalI AAGNNNNNCTT 2 cut(s) 499, 531
FaqI GGGAC 5 cut(s) 320, 393, 526, 693, 1431
FatI CATG 8 cut(s) 615, 952, 1074, 1155, 1290, 1486, 1506, 1660
FauI CCCGC 1 cut(s) 775
FbaI TGATCA 1 cut(s) 1279
Fnu4HI GCNGC 9 cut(s) 6, 9, 63, 66, 159, 162, 323, 563, 1128
FokI GGATG 5 cut(s) 40, 827, 1148, 1196, 1259
FriOI GRGCYC 2 cut(s) 187, 1558
Fsp4HI GCNGC 9 cut(s) 6, 9, 63, 66, 159, 162, 323, 563, 1128
FspBI CTAG 4 cut(s) 893, 1340, 1491, 1752
GlaI GCGC 6 cut(s) 157, 1229, 1289, 1318, 1681, 1748
GluI GCNGC 9 cut(s) 6, 9, 63, 66, 159, 162, 323, 563, 1128
GsaI CCCAGC 1 cut(s) 1227
GsuI CTGGAG 1 cut(s) 12
HaeII RGCGCY 1 cut(s) 1231
HaeIII GGCC 4 cut(s) 143, 842, 1344, 1379
HapII CCGG 3 cut(s) 83, 203, 1534
HgaI GACGC 2 cut(s) 1294, 1730
HhaI GCGC 6 cut(s) 158, 1230, 1290, 1319, 1682, 1749
Hin1I GRCGYC 1 cut(s) 1228
Hin1II CATG 8 cut(s) 619, 956, 1078, 1159, 1294, 1490, 1510, 1664
Hin6I GCGC 6 cut(s) 156, 1228, 1288, 1317, 1680, 1747
HinP1I GCGC 6 cut(s) 156, 1228, 1288, 1317, 1680, 1747
HinfI GANTC 9 cut(s) 214, 407, 575, 965, 1007, 1044, 1457, 1466, 1474
HpaII CCGG 3 cut(s) 83, 203, 1534
HphI GGTGA 4 cut(s) 28, 533, 1316, 1346
Hpy166II GTNNAC 4 cut(s) 808, 1359, 1603, 1613
Hpy188I TCNGA 9 cut(s) 182, 396, 430, 574, 977, 1102, 1284, 1432, 1651
Hpy188III TCNNGA 5 cut(s) 235, 250, 278, 292, 442
Hpy8I GTNNAC 4 cut(s) 808, 1359, 1603, 1613
HpyAV CCTTC 9 cut(s) 57, 247, 297, 451, 665, 1108, 1237, 1270, 1408
HpyCH4III ACNGT 7 cut(s) 538, 650, 992, 1027, 1136, 1610, 1699
HpyCH4V TGCA 7 cut(s) 581, 670, 803, 1130, 1155, 1486, 1660
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 155, 182, 930, 1136, 1323, 1595
HpyF3I CTNAG 8 cut(s) 137, 375, 393, 452, 501, 1429, 1581, 1688
Hsp92I GRCGYC 1 cut(s) 1228
Hsp92II CATG 8 cut(s) 619, 956, 1078, 1159, 1294, 1490, 1510, 1664
HspAI GCGC 6 cut(s) 156, 1228, 1288, 1317, 1680, 1747
KasI GGCGCC 1 cut(s) 1227
KroI GCCGGC 1 cut(s) 202
KroNI GCCGGC 1 cut(s) 204
Ksp22I TGATCA 1 cut(s) 1279
Kzo9I GATC 7 cut(s) 261, 461, 550, 1279, 1336, 1541, 1651
LmnI GCTCC 6 cut(s) 46, 91, 176, 182, 1561, 1664
Lsp1109I GCAGC 5 cut(s) 145, 148, 309, 549, 1114
LweI GCATC 1 cut(s) 319
MaeI CTAG 4 cut(s) 893, 1340, 1491, 1752
MaeIII GTNAC 4 cut(s) 275, 883, 911, 1036
MalI GATC 7 cut(s) 263, 463, 552, 1281, 1338, 1543, 1653
MbiI CCGCTC 2 cut(s) 41, 176
MboI GATC 7 cut(s) 261, 461, 550, 1279, 1336, 1541, 1651
MboII GAAGA 6 cut(s) 271, 990, 1016, 1244, 1285, 1423
MflI RGATCY 2 cut(s) 461, 1651
MhlI GDGCHC 2 cut(s) 187, 1558
Mly113I GGCGCC 1 cut(s) 1228
MlyI GAGTC 2 cut(s) 208, 1466
Mph1103I ATGCAT 1 cut(s) 1488
MroNI GCCGGC 1 cut(s) 202
MroXI GAANNNNTTC 2 cut(s) 790, 1118
MseI TTAA 5 cut(s) 210, 336, 401, 474, 789
MslI CAYNNNNRTG 2 cut(s) 813, 836
MspA1I CMGCKG 1 cut(s) 1364
MspCI CTTAAG 1 cut(s) 400
MspI CCGG 3 cut(s) 83, 203, 1534
MspR9I CCNGG 5 cut(s) 83, 812, 845, 1534, 1625
Mva1269I GAATGC 1 cut(s) 1178
MvaI CCWGG 3 cut(s) 812, 845, 1625
MvnI CGCG 4 cut(s) 156, 1288, 1317, 1319
MwoI GCNNNNNNNGC 7 cut(s) 38, 155, 182, 930, 1136, 1323, 1595
NaeI GCCGGC 1 cut(s) 204
NarI GGCGCC 1 cut(s) 1228
NciI CCSGG 2 cut(s) 83, 1534
NdeII GATC 7 cut(s) 261, 461, 550, 1279, 1336, 1541, 1651
NgoMIV GCCGGC 1 cut(s) 202
NlaIII CATG 8 cut(s) 619, 956, 1078, 1159, 1294, 1490, 1510, 1664
NlaIV GGNNCC 9 cut(s) 87, 172, 201, 381, 463, 735, 1229, 1557, 1666
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 3 cut(s) 275, 883, 911
NsiI ATGCAT 1 cut(s) 1488
NspI RCATGY 3 cut(s) 1078, 1294, 1490
OliI CACNNNNGTG 1 cut(s) 836
PaeI GCATGC 1 cut(s) 1490
PasI CCCWGGG 1 cut(s) 844
PcsI WCGNNNNNNNCGW 2 cut(s) 203, 276
PctI GAATGC 1 cut(s) 1178
PdiI GCCGGC 1 cut(s) 204
PdmI GAANNNNTTC 2 cut(s) 790, 1118
PfeI GAWTC 7 cut(s) 407, 575, 965, 1007, 1044, 1466, 1474
PflMI CCANNNNNTGG 1 cut(s) 1661
PkrI GCNGC 9 cut(s) 7, 10, 64, 67, 160, 163, 324, 564, 1129
PleI GAGTC 2 cut(s) 208, 1465
PluTI GGCGCC 1 cut(s) 1231
PpsI GAGTC 2 cut(s) 208, 1465
PpuMI RGGWCCY 2 cut(s) 380, 1627
PshBI ATTAAT 1 cut(s) 789
PsiI TTATAA 1 cut(s) 1017
Psp124BI GAGCTC 1 cut(s) 187
Psp5II RGGWCCY 2 cut(s) 380, 1627
Psp6I CCWGG 3 cut(s) 810, 843, 1623
PspFI CCCAGC 1 cut(s) 1223
PspGI CCWGG 3 cut(s) 810, 843, 1623
PspN4I GGNNCC 9 cut(s) 87, 172, 201, 381, 463, 735, 1229, 1557, 1666
PspPI GGNCC 8 cut(s) 141, 380, 734, 841, 852, 1056, 1343, 1627
PspPPI RGGWCCY 2 cut(s) 380, 1627
PstNI CAGNNNCTG 1 cut(s) 1715
PsuI RGATCY 2 cut(s) 461, 1651
PvuII CAGCTG 1 cut(s) 1364
RsaI GTAC 1 cut(s) 1612
RsaNI GTAC 1 cut(s) 1611
RseI CAYNNNNRTG 2 cut(s) 813, 836
SacI GAGCTC 1 cut(s) 187
SaqAI TTAA 5 cut(s) 210, 336, 401, 474, 789
SatI GCNGC 9 cut(s) 6, 9, 63, 66, 159, 162, 323, 563, 1128
Sau3AI GATC 7 cut(s) 261, 461, 550, 1279, 1336, 1541, 1651
Sau96I GGNCC 8 cut(s) 141, 380, 734, 841, 852, 1056, 1343, 1627
SchI GAGTC 2 cut(s) 208, 1466
ScrFI CCNGG 5 cut(s) 83, 812, 845, 1534, 1625
SduI GDGCHC 2 cut(s) 187, 1558
SfaNI GCATC 1 cut(s) 319
SfoI GGCGCC 1 cut(s) 1229
SinI GGWCC 5 cut(s) 380, 734, 852, 1056, 1627
SmiMI CAYNNNNRTG 2 cut(s) 813, 836
SmlI CTYRAG 3 cut(s) 387, 400, 541
SmoI CTYRAG 3 cut(s) 387, 400, 541
SphI GCATGC 1 cut(s) 1490
SspDI GGCGCC 1 cut(s) 1227
SspI AATATT 1 cut(s) 606
SspMI CTAG 4 cut(s) 893, 1340, 1491, 1752
SstI GAGCTC 1 cut(s) 187
StyD4I CCNGG 5 cut(s) 81, 810, 843, 1532, 1623
StyI CCWWGG 3 cut(s) 760, 862, 1068
TaaI ACNGT 7 cut(s) 538, 650, 992, 1027, 1136, 1610, 1699
TaqI TCGA 2 cut(s) 234, 1122
TauI GCSGC 3 cut(s) 8, 65, 68
TfiI GAWTC 7 cut(s) 407, 575, 965, 1007, 1044, 1466, 1474
Tru1I TTAA 5 cut(s) 210, 336, 401, 474, 789
Tru9I TTAA 5 cut(s) 210, 336, 401, 474, 789
TseFI GTSAC 3 cut(s) 275, 883, 911
TseI GCWGC 6 cut(s) 8, 158, 161, 322, 562, 1127
Tsp45I GTSAC 3 cut(s) 275, 883, 911
TspDTI ATGAA 7 cut(s) 459, 492, 632, 941, 1185, 1458, 1495
TspGWI ACGGA 1 cut(s) 844
Van91I CCANNNNNTGG 1 cut(s) 1661
Vha464I CTTAAG 1 cut(s) 400
VpaK11BI GGWCC 5 cut(s) 380, 734, 852, 1056, 1627
VspI ATTAAT 1 cut(s) 789
XapI RAATTY 1 cut(s) 1118
XceI RCATGY 3 cut(s) 1078, 1294, 1490
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmnI GAANNNNTTC 2 cut(s) 790, 1118
XspI CTAG 4 cut(s) 893, 1340, 1491, 1752
Zsp2I ATGCAT 1 cut(s) 1488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.