Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0009472

Physical Location & Seq

Genomic Coordinates Reverse (-)

28026599 .. 28031024

Gene Structure 4426 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1231900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1569 bp

ATGGTGTCAATGCCGTCCATTAAGCCCCCTCCTCTCGATTTCGATACCTCTGTTTTCACCAAGGAGAAGATCAACCTCGCTGGTCACAACGAGTATATTGTGAGAGGAGGAAGGGATCTGTTCCATTTGCTGCCCGATGCTTTCAAGGGTATTAAGCAGATTGGTGTTATCGGTTGGGGTTCTCAGGGACCTGCTCAAGCTCAGAATCTAAGAGATTCTCTAGTTGAAGCAAAGTCTGACATTGTTGTCAAGATTGGACTCAGGAAGGGTTCTCATTCTTTTGCTGAAGCTCGTGCAGCTGGTTTCACCGAGGAGAATGGGACTTTGGGGGATATATGGGAAACAATCTCAGGCAGTAATCTCGTGTTGCTACTAATCTCTGATTCTGCACAGGCCGATAATTATGAGAAAATATTTTCACACATGAAACCAAATAGTATACTTGGTCTTTCCCATGGTTTTCTTCTAGGGCATTTGCAGTCAGTGGGACTTGACTTTCCCAAGAACTTCAGTGTAATTGCTGTATGTCCCAAGGGGATGGGTCCATCTGTAAGGAGACTTTATGTCCAAGGCAAAGAGATAAATGGTGCCGGAATTAATTCCAGTTTTGCAGTCCACCAGGATGTTGATGGTAGAGCCACAGATGTGGCCCTGGGCTGGTCAGTTGCCCTTGGTTCTCCTTTCACATTTGCCACTACACTAGAGCAGGAATACAAAAGTGACATCTTTGGTGAACGAGGCATATTACTTGGTGCTGTTCATGGAATTGTGGAGTCCCTGTTTAGAAGGTACACTGAAAATGGAATGAGTGAGGATTTGGCTTATAAAAACACTGTTGAATGTATAACTGGAATTGTGTCAAGGACCATCTCAACTAAGGGAATGTTAGAGATTTACAACTCTTTGTCTGAAGATGGCAAAAAGGAATTTGAGGCTGCATATAGCGCCTCATTTTATCCATGCATGGACATCCTGTATGAATGCTATGAAGATGTTGCCAGTGGCAGCGAGATCCGCAGTGTTGTCTTGGCCGGTCGTCGCTTTTATGAGAAGGAGGGTCTGCCTGCATTCCCAATGGGTAAAATTGATCAGACCCGCATGTGGAAAGTCGGTGAGCGTGTCAGATCAACAAGGCCAGCTGGTGATCTAGGCCCGTTACACCCTTTCACTGCAGGTGTTTTTGTTGCCTTAATGATGGCGCAGATCGAAGTATTGAGGAAGAAAGGACATTCATATTCAGAGATCATTAATGAAAGTGTTATTGAGTCGGTGGATTCTTTGAATCCTTTTATGCATGCACGGGGAGTTTCTTTCATGGTTGATAACTGTTCAACAACAGCTCGGTTGGGATCAAGGAAATGGGCTCCACGATTTGATTACATCCTTACTCAGCAGGCCCTAGTTTCCGTGGACAATGGTGCACCCATCAACCGAGACCTCATCAGCAACTTCATGTCAGATCCAGTGCATGGAGCCATCAAAGTATGCGCTGAACTGAGACCTACAGTTGACATTTCAGTGACTGCTGATGCAGATTTTGTGCGACCAGAGTTGCGACAGAGCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

IlvC IlvN

Protein Analysis

523

Amino Acids

57.17

Weight (kDa)

5.9

Isoelectric Point (pI)

37.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KARI_N	PF07991	52 - 226	7.5e-30	Acetohydroxy acid isomeroreductase, NADPH-binding domain
KARI_C	PF01450	235 - 379	4.5e-34	Ketol-acid reductoisomerase, C-terminal domain
KARI_C	PF01450	391 - 461	3.8e-07	Ketol-acid reductoisomerase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009472)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58610 AT3G58610 AT3G58610
fragaria_vesca	FvH4_6g27150 FvH4_6g27150
malus_domestica	MD09G1239300.v1.1 MD17G1231900.v1.1 MD17G1232500.v1.1
prunus_persica	Prupe.3G122300_v2.0.a1 Prupe.3G122300_v2.0.a1
pyrus_communis	pycom09g15800 pycom17g23520
rosa_chinensis	RchiOBHm_Chr2g0129141
rosa_laevigata	RLG00000019058 RLG00000019060
rosa_multiflora	Rmu_sc0003479.1_g000002
rosa_roxburghii	Rroxscaffold_2G00114800
rosa_rugosa	Rorug02G0285100
rosa_samantha	Rh2BG345200 Rh2CG324600 Rh2DG362000
rosa_wichuraiana	Rw2G027350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	825
AarI	CACCTGC	1 cut(s)	1163
AasI	GACNNNNNNGTC	1 cut(s)	245
Acc36I	ACCTGC	2 cut(s)	199, 1163
AccB1I	GGYRCC	1 cut(s)	587
AccB7I	CCANNNNNTGG	1 cut(s)	1469
AccI	GTMKAC	1 cut(s)	439
AciI	CCGC	2 cut(s)	1015, 1096
AclWI	GGATC	4 cut(s)	123, 1006, 1357, 1454
AcoI	YGGCCR	1 cut(s)	1029
AcsI	RAATTY	1 cut(s)	926
AcuI	CTGAAG	3 cut(s)	306, 493, 930
AfaI	GTAC	1 cut(s)	791
AfiI	CCNNNNNNNGG	3 cut(s)	657, 1101, 1469
AgsI	TTSAA	5 cut(s)	145, 227, 839, 1282, 1332
AhdI	GACNNNNNGTC	1 cut(s)	563
AjnI	CCWGG	2 cut(s)	618, 651
AjuI	GAANNNNNNNTTGG	2 cut(s)	308, 340
AleI	CACNNNNGTG	1 cut(s)	644
AluBI	AGCT	5 cut(s)	200, 290, 299, 1139, 1340
AluI	AGCT	5 cut(s)	200, 290, 299, 1139, 1340
Alw21I	GWGCWC	1 cut(s)	1423
Alw26I	GTCTC	3 cut(s)	550, 1428, 1492
Alw44I	GTGCAC	1 cut(s)	1419
AlwI	GGATC	4 cut(s)	123, 1006, 1357, 1454
AlwNI	CAGNNNCTG	1 cut(s)	1523
AoxI	GGCC	6 cut(s)	393, 648, 1029, 1133, 1150, 1395
ApaLI	GTGCAC	1 cut(s)	1419
ApeKI	GCWGC	4 cut(s)	130, 296, 935, 1005
ApoI	RAATTY	1 cut(s)	926
AseI	ATTAAT	2 cut(s)	597, 1248
Asp700I	GAANNNNTTC	1 cut(s)	598
AspLEI	GCGC	3 cut(s)	947, 1201, 1490
AspS9I	GGNCC	6 cut(s)	188, 542, 649, 864, 1151, 1396
AsuHPI	GGTGA	5 cut(s)	49, 298, 743, 1124, 1154
AvaII	GGWCC	3 cut(s)	188, 542, 864
BaeGI	GKGCMC	1 cut(s)	1423
BaeI	ACNNNNGTAYC	2 cut(s)	36, 69
BanI	GGYRCC	1 cut(s)	587
BanII	GRGCYC	1 cut(s)	1366
BauI	CACGAG	2 cut(s)	291, 362
Bbv12I	GWGCWC	1 cut(s)	1423
BbvI	GCAGC	4 cut(s)	117, 308, 922, 1017
BccI	CCATC	8 cut(s)	532, 553, 623, 875, 908, 1189, 1433, 1484
BcgI	CGANNNNNNTGC	2 cut(s)	343, 377
BciT130I	CCWGG	2 cut(s)	620, 653
BclI	TGATCA	1 cut(s)	1087
BcoDI	GTCTC	3 cut(s)	550, 1428, 1492
BfaI	CTAG	5 cut(s)	221, 467, 701, 1148, 1400
BfmI	CTRYAG	2 cut(s)	1170, 1503
BfoI	RGCGCY	1 cut(s)	948
BfuAI	ACCTGC	2 cut(s)	199, 1163
BisI	GCNGC	4 cut(s)	131, 297, 936, 1006
BlsI	GCNGC	4 cut(s)	132, 298, 937, 1007
Bme1390I	CCNGG	2 cut(s)	620, 653
Bme18I	GGWCC	3 cut(s)	188, 542, 864
BmeRI	GACNNNNNGTC	1 cut(s)	563
BmgT120I	GGNCC	6 cut(s)	188, 542, 649, 864, 1151, 1396
BmiI	GGNNCC	5 cut(s)	189, 543, 589, 1365, 1474
BmrFI	CCNGG	2 cut(s)	620, 653
BmsI	GCATC	2 cut(s)	127, 1519
BpuEI	CTTGAG	1 cut(s)	180
BsaI	GGTCTC	2 cut(s)	1428, 1492
BsaJI	CCNNGG	9 cut(s)	60, 309, 454, 531, 568, 651, 652, 670, 1407
BsaXI	ACNNNNNCTCC	2 cut(s)	764, 794
Bsc4I	CCNNNNNNNGG	3 cut(s)	657, 1101, 1469
Bse118I	RCCGGY	1 cut(s)	1031
Bse1I	ACTGG	4 cut(s)	603, 853, 999, 1463
BseBI	CCWGG	2 cut(s)	620, 653
BseDI	CCNNGG	9 cut(s)	60, 309, 454, 531, 568, 651, 652, 670, 1407
BseGI	GGATG	4 cut(s)	543, 628, 969, 1380
BseLI	CCNNNNNNNGG	3 cut(s)	657, 1101, 1469
BseMII	CTCAG	6 cut(s)	197, 215, 274, 363, 1403, 1487
BseNI	ACTGG	4 cut(s)	603, 853, 999, 1463
BseRI	GAGGAG	3 cut(s)	21, 120, 326
BseSI	GKGCMC	1 cut(s)	1423
BseXI	GCAGC	4 cut(s)	117, 308, 922, 1017
BsgI	GTGCAG	2 cut(s)	315, 372
Bsh1285I	CGRYCG	1 cut(s)	1036
BshFI	GGCC	6 cut(s)	395, 650, 1031, 1135, 1152, 1397
BshNI	GGYRCC	1 cut(s)	587
BsiEI	CGRYCG	1 cut(s)	1036
BsiHKAI	GWGCWC	1 cut(s)	1423
BsiSI	CCGG	2 cut(s)	591, 1032
BslFI	GGGAC	5 cut(s)	201, 334, 501, 513, 760
BslI	CCNNNNNNNGG	3 cut(s)	657, 1101, 1469
BsmAI	GTCTC	3 cut(s)	550, 1428, 1492
BsmFI	GGGAC	5 cut(s)	201, 334, 501, 513, 760
BsmI	GAATGC	2 cut(s)	986, 1067
BsnI	GGCC	6 cut(s)	395, 650, 1031, 1135, 1152, 1397
Bso31I	GGTCTC	2 cut(s)	1428, 1492
Bsp1286I	GDGCHC	2 cut(s)	1366, 1423
Bsp19I	CCATGG	1 cut(s)	454
BspACI	CCGC	2 cut(s)	1015, 1096
BspANI	GGCC	6 cut(s)	395, 650, 1031, 1135, 1152, 1397
BspCNI	CTCAG	6 cut(s)	196, 214, 273, 362, 1402, 1488
BspLI	GGNNCC	5 cut(s)	189, 543, 589, 1365, 1474
BspMAI	CTGCAG	1 cut(s)	1174
BspMI	ACCTGC	2 cut(s)	199, 1163
BspPI	GGATC	4 cut(s)	123, 1006, 1357, 1454
BspT107I	GGYRCC	1 cut(s)	587
BspTNI	GGTCTC	2 cut(s)	1428, 1492
BsrFI	RCCGGY	1 cut(s)	1031
BsrI	ACTGG	4 cut(s)	603, 853, 999, 1463
BssAI	RCCGGY	1 cut(s)	1031
BssECI	CCNNGG	9 cut(s)	60, 309, 454, 531, 568, 651, 652, 670, 1407
BssNAI	GTATAC	1 cut(s)	440
BssSI	CACGAG	2 cut(s)	291, 362
BssT1I	CCWWGG	5 cut(s)	60, 454, 531, 568, 670
Bst1107I	GTATAC	1 cut(s)	440
Bst2BI	CACGAG	2 cut(s)	291, 362
Bst2UI	CCWGG	2 cut(s)	620, 653
Bst4CI	ACNGT	3 cut(s)	835, 1328, 1507
BstC8I	GCNNGC	4 cut(s)	1065, 1137, 1296, 1395
BstDEI	CTNAG	8 cut(s)	183, 201, 209, 260, 349, 876, 1389, 1496
BstDSI	CCRYGG	2 cut(s)	454, 1407
BstF5I	GGATG	4 cut(s)	543, 628, 969, 1380
BstH2I	RGCGCY	1 cut(s)	948
BstHHI	GCGC	3 cut(s)	947, 1201, 1490
BstMAI	GTCTC	3 cut(s)	550, 1428, 1492
BstMCI	CGRYCG	1 cut(s)	1036
BstMWI	GCNNNNNNNGC	3 cut(s)	296, 944, 1014
BstNI	CCWGG	2 cut(s)	620, 653
BstNSI	RCATGY	2 cut(s)	1102, 1298
BstSCI	CCNGG	2 cut(s)	618, 651
BstSFI	CTRYAG	2 cut(s)	1170, 1503
BstSLI	GKGCMC	1 cut(s)	1423
BstV1I	GCAGC	4 cut(s)	117, 308, 922, 1017
BstX2I	RGATCY	3 cut(s)	115, 1011, 1459
BstXI	CCANNNNNNTGG	2 cut(s)	538, 646
BstYI	RGATCY	3 cut(s)	115, 1011, 1459
BstZ17I	GTATAC	1 cut(s)	440
BsuRI	GGCC	6 cut(s)	395, 650, 1031, 1135, 1152, 1397
BtgI	CCRYGG	2 cut(s)	454, 1407
BtsCI	GGATG	4 cut(s)	543, 628, 969, 1380
BtsI	GCAGTG	2 cut(s)	1024, 1167
BtsIMutI	CAGTG	9 cut(s)	489, 517, 792, 831, 1006, 1024, 1167, 1470, 1524
BveI	ACCTGC	2 cut(s)	199, 1163
Cac8I	GCNNGC	4 cut(s)	1065, 1137, 1296, 1395
CaiI	CAGNNNCTG	1 cut(s)	1523
CfoI	GCGC	3 cut(s)	947, 1201, 1490
Cfr10I	RCCGGY	1 cut(s)	1031
Cfr13I	GGNCC	6 cut(s)	188, 542, 649, 864, 1151, 1396
Csp6I	GTAC	1 cut(s)	790
CviQI	GTAC	1 cut(s)	790
DdeI	CTNAG	8 cut(s)	183, 201, 209, 260, 349, 876, 1389, 1496
DrdI	GACNNNNNNGTC	1 cut(s)	245
DriI	GACNNNNNGTC	1 cut(s)	563
DseDI	GACNNNNNNGTC	1 cut(s)	245
EaeI	YGGCCR	1 cut(s)	1029
Eam1105I	GACNNNNNGTC	1 cut(s)	563
Eco130I	CCWWGG	5 cut(s)	60, 454, 531, 568, 670
Eco24I	GRGCYC	1 cut(s)	1366
Eco31I	GGTCTC	2 cut(s)	1428, 1492
Eco47I	GGWCC	3 cut(s)	188, 542, 864
Eco57I	CTGAAG	3 cut(s)	306, 493, 930
EcoO109I	RGGNCCY	2 cut(s)	188, 1396
EcoRII	CCWGG	2 cut(s)	618, 651
EcoT14I	CCWWGG	5 cut(s)	60, 454, 531, 568, 670
EcoT22I	ATGCAT	2 cut(s)	965, 1296
EcoT38I	GRGCYC	1 cut(s)	1366
ErhI	CCWWGG	5 cut(s)	60, 454, 531, 568, 670
FaqI	GGGAC	5 cut(s)	201, 334, 501, 513, 760
FauI	CCCGC	1 cut(s)	1103
FbaI	TGATCA	1 cut(s)	1087
FblI	GTMKAC	1 cut(s)	439
Fnu4HI	GCNGC	4 cut(s)	131, 297, 936, 1006
FokI	GGATG	4 cut(s)	550, 635, 956, 1367
FriOI	GRGCYC	1 cut(s)	1366
Fsp4HI	GCNGC	4 cut(s)	131, 297, 936, 1006
FspBI	CTAG	5 cut(s)	221, 467, 701, 1148, 1400
GlaI	GCGC	3 cut(s)	946, 1200, 1489
GluI	GCNGC	4 cut(s)	131, 297, 936, 1006
HaeII	RGCGCY	1 cut(s)	948
HaeIII	GGCC	6 cut(s)	395, 650, 1031, 1135, 1152, 1397
HapII	CCGG	2 cut(s)	591, 1032
HhaI	GCGC	3 cut(s)	947, 1201, 1490
Hin6I	GCGC	3 cut(s)	945, 1199, 1488
HinP1I	GCGC	3 cut(s)	945, 1199, 1488
HincII	GTYRAC	1 cut(s)	1510
HindII	GTYRAC	1 cut(s)	1510
HinfI	GANTC	8 cut(s)	205, 215, 258, 383, 773, 1265, 1274, 1282
HpaII	CCGG	2 cut(s)	591, 1032
HphI	GGTGA	5 cut(s)	49, 298, 743, 1124, 1154
Hpy166II	GTNNAC	7 cut(s)	440, 616, 734, 792, 1411, 1421, 1510
Hpy188I	TCNGA	8 cut(s)	204, 238, 382, 910, 1092, 1124, 1240, 1459
Hpy188III	TCNNGA	3 cut(s)	35, 250, 262
Hpy8I	GTNNAC	7 cut(s)	440, 616, 734, 792, 1411, 1421, 1510
Hpy99I	CGWCG	1 cut(s)	1041
HpyAV	CCTTC	4 cut(s)	105, 259, 780, 1045
HpyCH4III	ACNGT	3 cut(s)	835, 1328, 1507
HpyF10VI	GCNNNNNNNGC	3 cut(s)	296, 944, 1014
HpyF3I	CTNAG	8 cut(s)	183, 201, 209, 260, 349, 876, 1389, 1496
HspAI	GCGC	3 cut(s)	945, 1199, 1488
Ksp22I	TGATCA	1 cut(s)	1087
LmnI	GCTCC	2 cut(s)	1369, 1472
Lsp1109I	GCAGC	4 cut(s)	117, 308, 922, 1017
LweI	GCATC	2 cut(s)	127, 1519
MaeI	CTAG	5 cut(s)	221, 467, 701, 1148, 1400
MaeIII	GTNAC	4 cut(s)	83, 719, 1155, 1519
MboII	GAAGA	5 cut(s)	79, 455, 923, 1001, 1231
MflI	RGATCY	3 cut(s)	115, 1011, 1459
MhlI	GDGCHC	2 cut(s)	1366, 1423
MluCI	AATT	8 cut(s)	400, 516, 594, 598, 765, 852, 926, 1083
MlyI	GAGTC	3 cut(s)	252, 782, 1274
Mph1103I	ATGCAT	2 cut(s)	965, 1296
MroXI	GAANNNNTTC	1 cut(s)	598
MseI	TTAA	5 cut(s)	21, 153, 597, 1190, 1248
MslI	CAYNNNNRTG	3 cut(s)	621, 644, 1517
MspA1I	CMGCKG	2 cut(s)	299, 1139
MspI	CCGG	2 cut(s)	591, 1032
MspR9I	CCNGG	2 cut(s)	620, 653
Mva1269I	GAATGC	2 cut(s)	986, 1067
MvaI	CCWGG	2 cut(s)	620, 653
MwoI	GCNNNNNNNGC	3 cut(s)	296, 944, 1014
NcoI	CCATGG	1 cut(s)	454
NlaIV	GGNNCC	5 cut(s)	189, 543, 589, 1365, 1474
NmuCI	GTSAC	3 cut(s)	83, 719, 1519
NsiI	ATGCAT	2 cut(s)	965, 1296
NspI	RCATGY	2 cut(s)	1102, 1298
OliI	CACNNNNGTG	1 cut(s)	644
PaeI	GCATGC	1 cut(s)	1298
PaqCI	CACCTGC	1 cut(s)	1163
PasI	CCCWGGG	1 cut(s)	652
PctI	GAATGC	2 cut(s)	986, 1067
PdmI	GAANNNNTTC	1 cut(s)	598
PfeI	GAWTC	5 cut(s)	205, 215, 383, 1274, 1282
PflMI	CCANNNNNTGG	1 cut(s)	1469
PkrI	GCNGC	4 cut(s)	132, 298, 937, 1007
PleI	GAGTC	3 cut(s)	252, 781, 1273
PpsI	GAGTC	3 cut(s)	252, 781, 1273
PpuMI	RGGWCCY	1 cut(s)	188
PshBI	ATTAAT	2 cut(s)	597, 1248
PsiI	TTATAA	1 cut(s)	825
Psp5II	RGGWCCY	1 cut(s)	188
Psp6I	CCWGG	2 cut(s)	618, 651
PspGI	CCWGG	2 cut(s)	618, 651
PspN4I	GGNNCC	5 cut(s)	189, 543, 589, 1365, 1474
PspPI	GGNCC	6 cut(s)	188, 542, 649, 864, 1151, 1396
PspPPI	RGGWCCY	1 cut(s)	188
PstI	CTGCAG	1 cut(s)	1174
PstNI	CAGNNNCTG	1 cut(s)	1523
PsuI	RGATCY	3 cut(s)	115, 1011, 1459
PvuII	CAGCTG	2 cut(s)	299, 1139
RsaI	GTAC	1 cut(s)	791
RsaNI	GTAC	1 cut(s)	790
RseI	CAYNNNNRTG	3 cut(s)	621, 644, 1517
SaqAI	TTAA	5 cut(s)	21, 153, 597, 1190, 1248
SatI	GCNGC	4 cut(s)	131, 297, 936, 1006
Sau96I	GGNCC	6 cut(s)	188, 542, 649, 864, 1151, 1396
SchI	GAGTC	3 cut(s)	252, 782, 1274
ScrFI	CCNGG	2 cut(s)	620, 653
SduI	GDGCHC	2 cut(s)	1366, 1423
SfaNI	GCATC	2 cut(s)	127, 1519
SfcI	CTRYAG	2 cut(s)	1170, 1503
SinI	GGWCC	3 cut(s)	188, 542, 864
SmiMI	CAYNNNNRTG	3 cut(s)	621, 644, 1517
SmlI	CTYRAG	1 cut(s)	195
SmoI	CTYRAG	1 cut(s)	195
SphI	GCATGC	1 cut(s)	1298
Sse9I	AATT	8 cut(s)	400, 516, 594, 598, 765, 852, 926, 1083
SsiI	CCGC	2 cut(s)	1015, 1096
SspI	AATATT	1 cut(s)	414
SspMI	CTAG	5 cut(s)	221, 467, 701, 1148, 1400
StyD4I	CCNGG	2 cut(s)	618, 651
StyI	CCWWGG	5 cut(s)	60, 454, 531, 568, 670
TaaI	ACNGT	3 cut(s)	835, 1328, 1507
TaqI	TCGA	3 cut(s)	36, 42, 1206
TasI	AATT	8 cut(s)	400, 516, 594, 598, 765, 852, 926, 1083
TfiI	GAWTC	5 cut(s)	205, 215, 383, 1274, 1282
Tru1I	TTAA	5 cut(s)	21, 153, 597, 1190, 1248
Tru9I	TTAA	5 cut(s)	21, 153, 597, 1190, 1248
TscAI	CASTG	9 cut(s)	489, 517, 799, 838, 1006, 1024, 1174, 1470, 1524
TseFI	GTSAC	3 cut(s)	83, 719, 1519
TseI	GCWGC	4 cut(s)	130, 296, 935, 1005
Tsp45I	GTSAC	3 cut(s)	83, 719, 1519
TspDTI	ATGAA	8 cut(s)	440, 749, 993, 1002, 1221, 1266, 1303, 1441
TspGWI	ACGGA	1 cut(s)	1396
TspRI	CASTG	9 cut(s)	489, 517, 799, 838, 1006, 1024, 1174, 1470, 1524
Van91I	CCANNNNNTGG	1 cut(s)	1469
VneI	GTGCAC	1 cut(s)	1419
VpaK11BI	GGWCC	3 cut(s)	188, 542, 864
VspI	ATTAAT	2 cut(s)	597, 1248
XapI	RAATTY	1 cut(s)	926
XceI	RCATGY	2 cut(s)	1102, 1298
XcmI	CCANNNNNNNNNTGG	1 cut(s)	626
XmiI	GTMKAC	1 cut(s)	439
XmnI	GAANNNNTTC	1 cut(s)	598
XspI	CTAG	5 cut(s)	221, 467, 701, 1148, 1400
Zsp2I	ATGCAT	2 cut(s)	965, 1296

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.