Rorug02G0285100

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009472
Physical Location & Seq
Reverse (-)
31038990 .. 31045326
6337 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0285100.1

Sequence Viewer

Length: 1512 bp
ATGAAAGGTGGATTTAACTATAAAATGCTGGGGAGACCAGTCTCTGTCACTTTGACTGCTCTTGTTTGTACAACCATATTACTTTTGGTTTGGGAGAAAAATCCTTTTGCGGATACTCTACTATTGGCCCGAGAACATTTTCCAATTCCTTCGATAGATTTTCTGGAGGACTCCTCCTCAAAATCTTTGAAGACAGATGAGAATGTTCAAGGGAAGGATTCACCTTCTGTTATAATAGAAGAAGCAAAAGAAATTGAAAATTCTGGTGCTGCACCAGTTCAAGGGAAGGGGTCACCTTCTATTATAATAGAAAAAACAAACAAAACACAAAATTCTGGTGCTGCACCAATTCAAGGGAATTCACCTTCTACTACAATAGAAGAAACAAACAAAATAGAAAACTCTGGTGCTGCACCGACAGATTTTGCATCTTCTCCTAGAGTGCATGAACATAATTCGAATGCGACATCTCCTCACCAACCTAAAGTCTGTAATTATGCCAAGGGTAGATGGATTCCAGACAGCAGGCGACCATTGTATTCTGGGTTTCATTGTAAGCAATGGTTATCAGCAATGTGGGCTTGCAGACTGACACAACGACCAGATTTCTCTTTTGAGGGATACCGGTGGAAGCCAGAAAGTTGTGAAATGCCAGAGTTCGAGCGATCTGCATTCTTGAGAAGAATGCAGGACAAGACAATTGCTTTTATAGGAGATTCTTTGGGCCGACAGCAGTTCCAGTCTTTAATGTGTATGGCAACCGGTGGAGAAGAAAGCCCAGAAGTGCAAAATGTGGGAAAAGAATATGGCCTTGTCAAACGTCGTGGAGCCATTCGTCCTGATGGCTGGGCCTATCGATTTCCGAAGACCAATACCACCATTTTGTATTACTGGTCAGCAAGCCTCAGTGATCTTGTGCCGCTTAACAGCTCAAACCGAACCACTGATATTGCGATGCATTTGGACCGTCCTCCAGCTTTCATGAGGCAGTACCTTGATCGGTTTGATGTGTTGGTTCTTAATACAGGGCATCACTGGAACAGGGGAAAAATTAATGGAAATCGGTGGGTAATGTATGTAGATGGAAAACCTAATGAAGATAAAAGGCGTGCACAAATTGACAGAGCAAAGAATTTTACCATACATAGTGTTGTCAGGTGGCTTGATTCACAACTTCCATCGCATCCCCACCTCAAAACATTCTTTAGGACCATCTCACCAAGGCATTTCTTTAATGGTGACTGGAACACTGGGGGGTCCTGTGATAATACTACTCCGATGTCTGGAGGAAGTGAAGTGGTCCAAGAAGGATCAAGTGATTTAATCATTGAGTCTGCTTTGAGAGGTACAAAGACAAAAATTTTGGACATCACTGCTCTTTCTCAATTGAGGGATGAAGGTCATGTATCCCGCTACACCATTAGAGGAACTGCTGCTGTAAATGATTGCTTGCACTGGTGCCTACCTGGAATTCCAGACACATGGAATGAACTTCTTATTGCACAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

503

Amino Acids

56.54

Weight (kDa)

8.46

Isoelectric Point (pI)

56.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 163 - 216 4e-15 PMR5 N terminal Domain
PC-Esterase PF13839 217 - 501 2.2e-74 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 233, 305
AccB1I GGYRCC 1 cut(s) 1458
AciI CCGC 3 cut(s) 110, 920, 1411
AclWI GGATC 1 cut(s) 1318
AcsI RAATTY 6 cut(s) 259, 331, 358, 1132, 1359, 1470
AfaI GTAC 3 cut(s) 70, 992, 1348
AfiI CCNNNNNNNGG 3 cut(s) 281, 353, 1283
AgeI ACCGGT 2 cut(s) 624, 761
AgsI TTSAA 5 cut(s) 190, 209, 257, 281, 353
AjnI CCWGG 1 cut(s) 1465
AluBI AGCT 2 cut(s) 930, 977
AluI AGCT 2 cut(s) 930, 977
Alw21I GWGCWC 1 cut(s) 1114
Alw26I GTCTC 2 cut(s) 28, 46
Alw44I GTGCAC 1 cut(s) 1110
AlwI GGATC 1 cut(s) 1318
AlwNI CAGNNNCTG 1 cut(s) 44
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 4 cut(s) 126, 724, 808, 849
ApaLI GTGCAC 1 cut(s) 1110
ApeKI GCWGC 4 cut(s) 269, 341, 410, 1433
ApoI RAATTY 6 cut(s) 259, 331, 358, 1132, 1359, 1470
ArsI GACNNNNNNTTYG 2 cut(s) 1345, 1377
AseI ATTAAT 1 cut(s) 1053
AsiGI ACCGGT 2 cut(s) 624, 761
Asp700I GAANNNNTTC 1 cut(s) 138
AspS9I GGNCC 7 cut(s) 127, 724, 849, 964, 1209, 1257, 1300
AsuHPI GGTGA 6 cut(s) 213, 285, 354, 467, 1209, 1250
AsuII TTCGAA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 4 cut(s) 964, 1209, 1257, 1300
BaeGI GKGCMC 1 cut(s) 1114
BanI GGYRCC 1 cut(s) 1458
BbsI GAAGAC 2 cut(s) 197, 872
Bbv12I GWGCWC 1 cut(s) 1114
BbvI GCAGC 4 cut(s) 256, 328, 397, 1420
BccI CCATC 5 cut(s) 504, 836, 1076, 1186, 1220
BcgI CGANNNNNNTGC 2 cut(s) 650, 684
BciT130I CCWGG 1 cut(s) 1467
BciVI GTATCC 3 cut(s) 106, 614, 1417
BcoDI GTCTC 2 cut(s) 28, 46
BfaI CTAG 1 cut(s) 438
BfuI GTATCC 3 cut(s) 106, 614, 1417
BisI GCNGC 5 cut(s) 270, 342, 411, 920, 1434
BlsI GCNGC 5 cut(s) 271, 343, 412, 921, 1435
Bme1390I CCNGG 1 cut(s) 1467
Bme18I GGWCC 4 cut(s) 964, 1209, 1257, 1300
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 7 cut(s) 127, 724, 849, 964, 1209, 1257, 1300
BmiI GGNNCC 3 cut(s) 829, 1258, 1460
BmrFI CCNGG 1 cut(s) 1467
BmrI ACTGGG 1 cut(s) 1260
BmsI GCATC 4 cut(s) 437, 945, 1039, 1192
BmuI ACTGGG 1 cut(s) 1260
BpiI GAAGAC 2 cut(s) 197, 872
BplI GAGNNNNNCTC 2 cut(s) 158, 190
BpmI CTGGAG 3 cut(s) 185, 957, 1305
Bpu14I TTCGAA 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 697
Bsa29I ATCGAT 1 cut(s) 856
BsaI GGTCTC 1 cut(s) 28
BsaJI CCNNGG 2 cut(s) 501, 1220
BsaWI WCCGGW 2 cut(s) 624, 761
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 353, 1283
Bse118I RCCGGY 2 cut(s) 624, 761
Bse1I ACTGG 8 cut(s) 38, 275, 739, 896, 1040, 1247, 1255, 1460
Bse3DI GCAATG 2 cut(s) 566, 579
BseBI CCWGG 1 cut(s) 1467
BseCI ATCGAT 1 cut(s) 856
BseDI CCNNGG 2 cut(s) 501, 1220
BseGI GGATG 2 cut(s) 1183, 1399
BseLI CCNNNNNNNGG 3 cut(s) 281, 353, 1283
BseMI GCAATG 2 cut(s) 566, 579
BseMII CTCAG 1 cut(s) 919
BseNI ACTGG 8 cut(s) 38, 275, 739, 896, 1040, 1247, 1255, 1460
BseRI GAGGAG 3 cut(s) 163, 166, 462
BseSI GKGCMC 1 cut(s) 1114
BseXI GCAGC 4 cut(s) 256, 328, 397, 1420
BseYI CCCAGC 2 cut(s) 28, 846
BsgI GTGCAG 3 cut(s) 255, 327, 396
BshFI GGCC 4 cut(s) 128, 726, 810, 851
BshNI GGYRCC 1 cut(s) 1458
BshTI ACCGGT 2 cut(s) 624, 761
BshVI ATCGAT 1 cut(s) 856
BsiHKAI GWGCWC 1 cut(s) 1114
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 2 cut(s) 625, 762
BslI CCNNNNNNNGG 3 cut(s) 281, 353, 1283
BsmAI GTCTC 2 cut(s) 28, 46
BsmI GAATGC 3 cut(s) 466, 671, 690
BsnI GGCC 4 cut(s) 128, 726, 810, 851
Bso31I GGTCTC 1 cut(s) 28
BsoBI CYCGRG 1 cut(s) 129
Bsp119I TTCGAA 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 1114
Bsp1407I TGTACA 1 cut(s) 68
Bsp143I GATC 4 cut(s) 665, 910, 997, 1310
BspACI CCGC 3 cut(s) 110, 920, 1411
BspANI GGCC 4 cut(s) 128, 726, 810, 851
BspCNI CTCAG 1 cut(s) 918
BspDI ATCGAT 1 cut(s) 856
BspHI TCATGA 1 cut(s) 981
BspLI GGNNCC 3 cut(s) 829, 1258, 1460
BspPI GGATC 1 cut(s) 1318
BspT104I TTCGAA 1 cut(s) 458
BspT107I GGYRCC 1 cut(s) 1458
BspTNI GGTCTC 1 cut(s) 28
BsrDI GCAATG 2 cut(s) 566, 579
BsrFI RCCGGY 2 cut(s) 624, 761
BsrGI TGTACA 1 cut(s) 68
BsrI ACTGG 8 cut(s) 38, 275, 739, 896, 1040, 1247, 1255, 1460
BssAI RCCGGY 2 cut(s) 624, 761
BssECI CCNNGG 2 cut(s) 501, 1220
BssMI GATC 4 cut(s) 665, 910, 997, 1310
BssT1I CCWWGG 2 cut(s) 501, 1220
Bst2UI CCWGG 1 cut(s) 1467
Bst4CI ACNGT 1 cut(s) 968
BstAUI TGTACA 1 cut(s) 68
BstBI TTCGAA 1 cut(s) 458
BstC8I GCNNGC 5 cut(s) 527, 583, 901, 1110, 1451
BstDEI CTNAG 1 cut(s) 905
BstEII GGTNACC 1 cut(s) 291
BstF5I GGATG 2 cut(s) 1183, 1399
BstKTI GATC 4 cut(s) 668, 913, 1000, 1313
BstMAI GTCTC 2 cut(s) 28, 46
BstMBI GATC 4 cut(s) 665, 910, 997, 1310
BstMWI GCNNNNNNNGC 1 cut(s) 578
BstNI CCWGG 1 cut(s) 1467
BstPI GGTNACC 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 1465
BstSLI GKGCMC 1 cut(s) 1114
BstV1I GCAGC 4 cut(s) 256, 328, 397, 1420
BstV2I GAAGAC 2 cut(s) 197, 872
BstXI CCANNNNNNTGG 1 cut(s) 1482
Bsu15I ATCGAT 1 cut(s) 856
BsuI GTATCC 3 cut(s) 106, 614, 1417
BsuRI GGCC 4 cut(s) 128, 726, 810, 851
BsuTUI ATCGAT 1 cut(s) 856
BtgZI GCGATG 2 cut(s) 968, 1164
BtsCI GGATG 2 cut(s) 1183, 1399
BtsI GCAGTG 1 cut(s) 1371
BtsIMutI CAGTG 6 cut(s) 913, 942, 1033, 1248, 1371, 1453
Cac8I GCNNGC 5 cut(s) 527, 583, 901, 1110, 1451
CaiI CAGNNNCTG 1 cut(s) 44
CciI TCATGA 1 cut(s) 981
Cfr10I RCCGGY 2 cut(s) 624, 761
Cfr13I GGNCC 7 cut(s) 127, 724, 849, 964, 1209, 1257, 1300
ClaI ATCGAT 1 cut(s) 856
Csp6I GTAC 3 cut(s) 69, 991, 1347
CspAI ACCGGT 2 cut(s) 624, 761
CspCI CAANNNNNGTGG 4 cut(s) 805, 840, 931, 966
CviAII CATG 4 cut(s) 446, 982, 1403, 1482
CviQI GTAC 3 cut(s) 69, 991, 1347
DdeI CTNAG 1 cut(s) 905
DpnI GATC 4 cut(s) 667, 912, 999, 1312
DpnII GATC 4 cut(s) 665, 910, 997, 1310
Eco130I CCWWGG 2 cut(s) 501, 1220
Eco31I GGTCTC 1 cut(s) 28
Eco47I GGWCC 4 cut(s) 964, 1209, 1257, 1300
Eco88I CYCGRG 1 cut(s) 129
Eco91I GGTNACC 1 cut(s) 291
EcoO109I RGGNCCY 1 cut(s) 1257
EcoO65I GGTNACC 1 cut(s) 291
EcoRI GAATTC 2 cut(s) 358, 1470
EcoRII CCWGG 1 cut(s) 1465
EcoT14I CCWWGG 2 cut(s) 501, 1220
EcoT22I ATGCAT 1 cut(s) 960
ErhI CCWWGG 2 cut(s) 501, 1220
FaeI CATG 4 cut(s) 449, 985, 1406, 1485
FatI CATG 4 cut(s) 445, 981, 1402, 1481
FauI CCCGC 1 cut(s) 1418
Fnu4HI GCNGC 5 cut(s) 270, 342, 411, 920, 1434
FokI GGATG 2 cut(s) 1170, 1406
Fsp4HI GCNGC 5 cut(s) 270, 342, 411, 920, 1434
FspBI CTAG 1 cut(s) 438
GluI GCNGC 5 cut(s) 270, 342, 411, 920, 1434
GsaI CCCAGC 2 cut(s) 32, 850
GsuI CTGGAG 3 cut(s) 185, 957, 1305
HaeIII GGCC 4 cut(s) 128, 726, 810, 851
HapII CCGG 2 cut(s) 625, 762
Hin1II CATG 4 cut(s) 449, 985, 1406, 1485
HinfI GANTC 6 cut(s) 170, 218, 514, 716, 1166, 1331
HpaII CCGG 2 cut(s) 625, 762
HphI GGTGA 6 cut(s) 213, 285, 354, 467, 1209, 1250
Hpy166II GTNNAC 1 cut(s) 1112
Hpy188I TCNGA 2 cut(s) 864, 1278
Hpy188III TCNNGA 7 cut(s) 164, 518, 676, 839, 982, 1284, 1475
Hpy8I GTNNAC 1 cut(s) 1112
Hpy99I CGWCG 1 cut(s) 825
HpyAV CCTTC 8 cut(s) 159, 208, 234, 280, 306, 375, 1301, 1391
HpyCH4III ACNGT 1 cut(s) 968
HpyCH4IV ACGT 1 cut(s) 820
HpyF10VI GCNNNNNNNGC 1 cut(s) 578
HpyF3I CTNAG 1 cut(s) 905
HpySE526I ACGT 1 cut(s) 820
Hsp92II CATG 4 cut(s) 449, 985, 1406, 1485
Kzo9I GATC 4 cut(s) 665, 910, 997, 1310
LmnI GCTCC 1 cut(s) 827
Lsp1109I GCAGC 4 cut(s) 256, 328, 397, 1420
LweI GCATC 4 cut(s) 437, 945, 1039, 1192
MaeI CTAG 1 cut(s) 438
MaeII ACGT 1 cut(s) 820
MaeIII GTNAC 3 cut(s) 46, 291, 1238
MalI GATC 4 cut(s) 667, 912, 999, 1312
MboI GATC 4 cut(s) 665, 910, 997, 1310
MboII GAAGA 8 cut(s) 202, 251, 392, 423, 693, 782, 877, 1109
MfeI CAATTG 2 cut(s) 699, 1385
MhlI GDGCHC 1 cut(s) 1114
MlyI GAGTC 2 cut(s) 164, 1340
Mph1103I ATGCAT 1 cut(s) 960
MroXI GAANNNNTTC 1 cut(s) 138
MseI TTAA 7 cut(s) 15, 746, 924, 1020, 1053, 1233, 1322
MspI CCGG 2 cut(s) 625, 762
MspR9I CCNGG 1 cut(s) 1467
MunI CAATTG 2 cut(s) 699, 1385
Mva1269I GAATGC 3 cut(s) 466, 671, 690
MvaI CCWGG 1 cut(s) 1467
MwoI GCNNNNNNNGC 1 cut(s) 578
NdeII GATC 4 cut(s) 665, 910, 997, 1310
NlaIII CATG 4 cut(s) 449, 985, 1406, 1485
NlaIV GGNNCC 3 cut(s) 829, 1258, 1460
NmuCI GTSAC 3 cut(s) 46, 291, 1238
NsiI ATGCAT 1 cut(s) 960
NspV TTCGAA 1 cut(s) 458
PagI TCATGA 1 cut(s) 981
PctI GAATGC 3 cut(s) 466, 671, 690
PdmI GAANNNNTTC 1 cut(s) 138
PfeI GAWTC 4 cut(s) 218, 514, 716, 1166
PinAI ACCGGT 2 cut(s) 624, 761
PkrI GCNGC 5 cut(s) 271, 343, 412, 921, 1435
PleI GAGTC 2 cut(s) 164, 1339
PpsI GAGTC 2 cut(s) 164, 1339
PpuMI RGGWCCY 1 cut(s) 1257
PshBI ATTAAT 1 cut(s) 1053
PsiI TTATAA 2 cut(s) 233, 305
Psp5II RGGWCCY 1 cut(s) 1257
Psp6I CCWGG 1 cut(s) 1465
PspEI GGTNACC 1 cut(s) 291
PspFI CCCAGC 2 cut(s) 28, 846
PspGI CCWGG 1 cut(s) 1465
PspN4I GGNNCC 3 cut(s) 829, 1258, 1460
PspPI GGNCC 7 cut(s) 127, 724, 849, 964, 1209, 1257, 1300
PspPPI RGGWCCY 1 cut(s) 1257
PstNI CAGNNNCTG 1 cut(s) 44
RsaI GTAC 3 cut(s) 70, 992, 1348
RsaNI GTAC 3 cut(s) 69, 991, 1347
SaqAI TTAA 7 cut(s) 15, 746, 924, 1020, 1053, 1233, 1322
SatI GCNGC 5 cut(s) 270, 342, 411, 920, 1434
Sau3AI GATC 4 cut(s) 665, 910, 997, 1310
Sau96I GGNCC 7 cut(s) 127, 724, 849, 964, 1209, 1257, 1300
SchI GAGTC 2 cut(s) 164, 1340
ScrFI CCNGG 1 cut(s) 1467
SduI GDGCHC 1 cut(s) 1114
SfaNI GCATC 4 cut(s) 437, 945, 1039, 1192
SfuI TTCGAA 1 cut(s) 458
SinI GGWCC 4 cut(s) 964, 1209, 1257, 1300
SmlI CTYRAG 1 cut(s) 676
SmoI CTYRAG 1 cut(s) 676
SsiI CCGC 3 cut(s) 110, 920, 1411
SspMI CTAG 1 cut(s) 438
StyD4I CCNGG 1 cut(s) 1465
StyI CCWWGG 2 cut(s) 501, 1220
TaaI ACNGT 1 cut(s) 968
TaiI ACGT 1 cut(s) 823
TaqI TCGA 4 cut(s) 152, 458, 660, 856
TatI WGTACW 1 cut(s) 68
TauI GCSGC 1 cut(s) 922
TfiI GAWTC 4 cut(s) 218, 514, 716, 1166
Tru1I TTAA 7 cut(s) 15, 746, 924, 1020, 1053, 1233, 1322
Tru9I TTAA 7 cut(s) 15, 746, 924, 1020, 1053, 1233, 1322
TscAI CASTG 6 cut(s) 913, 949, 1040, 1255, 1378, 1460
TseFI GTSAC 3 cut(s) 46, 291, 1238
TseI GCWGC 4 cut(s) 269, 341, 410, 1433
Tsp45I GTSAC 3 cut(s) 46, 291, 1238
TspDTI ATGAA 7 cut(s) 17, 462, 539, 970, 1110, 1410, 1503
TspRI CASTG 6 cut(s) 913, 949, 1040, 1255, 1378, 1460
VneI GTGCAC 1 cut(s) 1110
VpaK11BI GGWCC 4 cut(s) 964, 1209, 1257, 1300
VspI ATTAAT 1 cut(s) 1053
XapI RAATTY 6 cut(s) 259, 331, 358, 1132, 1359, 1470
XcmI CCANNNNNNNNNTGG 1 cut(s) 82
XmnI GAANNNNTTC 1 cut(s) 138
XspI CTAG 1 cut(s) 438
Zsp2I ATGCAT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.