Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0009472

Physical Location & Seq

Genomic Coordinates Reverse (-)

40900834 .. 40904937

Gene Structure 4104 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00114800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

ATGCCGACGATTAAGCCCCCGCTGTCTCTTGATTTCGACACCTCTGTTTTCAACAAGGAGAAGATCAACCTCGCTGGTCACGATGAGTATATTGTAAAGGGAGGAAGGGACTTGTTCCATCTGCTGCCCGATGCTTTCAAGGGCATCAAGCAGATCGGTGTCATCGGTTGGGGTTCCCAGGGACCTGCTCAAGCTCAGAACTTGAGAGATTCTCTAGCTGAAGCAAAGTCGAATATTGTGGTCAAGATTGGGCTCAGGAAGGGATCCCGTTCATTTAATGAAGCTCGCGCAGCTGGTTTCACCGAGGAGAATGGGACTTTAGGTGATATATGGGAAACAATCTCAGAGAGTGATCTTGTGTTGCTACTAATCTCTGATTCTGCACAGGCAGATAATTATGAGAAAATATTTTCACACATGAAACCAAATAGCATACTTGGTCTTTCCCACGGTTTCCTTCTAGGGCATTTGCAGTCGGAGGGACTTGACTTTCCCAAGAACATCAGTGTAATTGCTGTATGCCCAAAGGGAATGGGTCCGTCAGTAAGGAGACTTTATGTCCAAGGCAAAGAAATAAATGGTGCGGGAATTAATTCAAGTTTTGCAGTCCACCAGGATGTTGATGGGAGAGCCACAGATGTGGCTCTGGGCTGGTCGGTTGCCCTTGGTTCTCCTTTCACATTTGTCACTACGCTAGAGCAGGAATACAAAAGTGACATCTTTGGCGAGCGAGGCATATTACTTGGTGCTGTTCATGGAATTGTGGAGTCCCTGTTCAGAAGATACACCGACAGCGGAATGAGTGAAGATTCTGCTTATAAAAATACTGTTGAATGTGTTACTGGAATTGTGTCAAGAACCATATCAACTAAGGGCATGCTGGAAATATACAGCTCTTTGTCTGAAGCTGACAAGAAGGAGTTCGAGACTGCATATAGTGCCTCCTATTATCCTTGCATGGACATCCTCTATGAATGCTATGAGGATGTAGCAAGTGGCAGTGAGATCCGCAGCGTTGTTTTGGCTGGGCGGCGATTTTATGAGAAAGATGGTCTTCCTGCCTTCCCTATGGGGAAGATTGATCAGACGCGCATGTGGAAAGTTGGTGAGCGTGTCCGATCGACTCGTCCAGCTGGTGATCTAGGCCCTCTACACCCTTTCACAGCTGGTGTTTTTGTGGCCCTGATGATGGCACAGATTGAAGTGTTAAGGAAGAAGGGGCACTCCTACTCAGAGATAATCAATGAAAGTGTAATTGAGTCGGTGGATTCTTTGAATCCTTTTATGCATGCTAGGGGAGTTTCTTTCATGGTTGATAATTGCTCAACAACAGCGCGGTTGGGATCAAGGAAATGGGCTCCAAGATTTGATTATATTTTAACTCAGCAAGCCTTGGTTGCCGTGGACAATGGTACACCCATCAATCAGGACCTCATTAGCAACTTCGTGTCAGATCCAGTGCATGGAGCCATTGAAGTATGTGCTCAACTGAGACCCACAGTTGATATTTCAGTGCCTGCTGATGCAGACTTTGTGCGACCAGAGTTGCGCCAATCTAGCAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

IlvC IlvN

Protein Analysis

521

Amino Acids

57.06

Weight (kDa)

5.5

Isoelectric Point (pI)

42.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KARI_N	PF07991	50 - 224	1.3e-31	Acetohydroxy acid isomeroreductase, NADPH-binding domain
KARI_C	PF01450	233 - 377	3.3e-34	Ketol-acid reductoisomerase, C-terminal domain
KARI_C	PF01450	389 - 458	3.8e-07	Ketol-acid reductoisomerase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009472)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58610 AT3G58610 AT3G58610
fragaria_vesca	FvH4_6g27150 FvH4_6g27150
malus_domestica	MD09G1239300.v1.1 MD17G1231900.v1.1 MD17G1232500.v1.1
prunus_persica	Prupe.3G122300_v2.0.a1 Prupe.3G122300_v2.0.a1
pyrus_communis	pycom09g15800 pycom17g23520
rosa_chinensis	RchiOBHm_Chr2g0129141
rosa_laevigata	RLG00000019058 RLG00000019060
rosa_multiflora	Rmu_sc0003479.1_g000002
rosa_roxburghii	Rroxscaffold_2G00114800
rosa_rugosa	Rorug02G0285100
rosa_samantha	Rh2BG345200 Rh2CG324600 Rh2DG362000
rosa_wichuraiana	Rw2G027350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	819
Acc36I	ACCTGC	1 cut(s)	193
AccB7I	CCANNNNNTGG	1 cut(s)	1463
AccII	CGCG	3 cut(s)	288, 1090, 1336
AciI	CCGC	6 cut(s)	20, 584, 795, 1009, 1030, 1336
AclWI	GGATC	5 cut(s)	258, 271, 1000, 1351, 1448
AcuI	CTGAAG	2 cut(s)	240, 924
AfaI	GTAC	1 cut(s)	1414
AfiI	CCNNNNNNNGG	2 cut(s)	1189, 1463
AgsI	TTSAA	7 cut(s)	52, 139, 597, 833, 1202, 1276, 1475
AhdI	GACNNNNNGTC	1 cut(s)	557
AjnI	CCWGG	2 cut(s)	177, 612
AleI	CACNNNNGTG	1 cut(s)	638
AloI	GAACNNNNNNTCC	2 cut(s)	758, 790
AluBI	AGCT	8 cut(s)	194, 218, 284, 293, 894, 908, 1133, 1166
AluI	AGCT	8 cut(s)	194, 218, 284, 293, 894, 908, 1133, 1166
Alw21I	GWGCWC	1 cut(s)	1486
Alw26I	GTCTC	4 cut(s)	30, 544, 920, 1486
AlwI	GGATC	5 cut(s)	258, 271, 1000, 1351, 1448
AlwNI	CAGNNNCTG	1 cut(s)	1517
AoxI	GGCC	2 cut(s)	1144, 1179
ApeKI	GCWGC	3 cut(s)	124, 290, 1011
AseI	ATTAAT	1 cut(s)	591
Asp700I	GAANNNNTTC	2 cut(s)	592, 920
AspLEI	GCGC	4 cut(s)	290, 1092, 1336, 1551
AspS9I	GGNCC	5 cut(s)	182, 536, 1145, 1180, 1429
AsuHPI	GGTGA	4 cut(s)	292, 335, 1118, 1148
AvaII	GGWCC	3 cut(s)	182, 536, 1429
BaeGI	GKGCMC	1 cut(s)	1224
BamHI	GGATCC	1 cut(s)	263
BanII	GRGCYC	2 cut(s)	255, 1360
BbsI	GAAGAC	1 cut(s)	1046
Bbv12I	GWGCWC	1 cut(s)	1486
BbvI	GCAGC	3 cut(s)	111, 302, 1023
BccI	CCATC	5 cut(s)	126, 617, 1043, 1183, 1427
BceAI	ACGGC	1 cut(s)	1385
BciT130I	CCWGG	2 cut(s)	179, 614
BclI	TGATCA	1 cut(s)	1081
BcoDI	GTCTC	4 cut(s)	30, 544, 920, 1486
BfaI	CTAG	6 cut(s)	215, 461, 695, 1142, 1293, 1557
BfuAI	ACCTGC	1 cut(s)	193
BisI	GCNGC	4 cut(s)	125, 291, 1012, 1031
BlsI	GCNGC	4 cut(s)	126, 292, 1013, 1032
Bme1390I	CCNGG	2 cut(s)	179, 614
Bme18I	GGWCC	3 cut(s)	182, 536, 1429
BmeRI	GACNNNNNGTC	1 cut(s)	557
BmgT120I	GGNCC	5 cut(s)	182, 536, 1145, 1180, 1429
BmiI	GGNNCC	6 cut(s)	175, 183, 265, 537, 1359, 1468
BmrFI	CCNGG	2 cut(s)	179, 614
BmsI	GCATC	3 cut(s)	121, 153, 1513
BpiI	GAAGAC	1 cut(s)	1046
BplI	GAGNNNNNCTC	2 cut(s)	196, 228
Bpu10I	CCTNAGC	1 cut(s)	254
BpuEI	CTTGAG	2 cut(s)	174, 223
BsaI	GGTCTC	1 cut(s)	1486
BsaJI	CCNNGG	8 cut(s)	177, 178, 303, 448, 562, 664, 1392, 1401
BsaXI	ACNNNNNCTCC	2 cut(s)	758, 788
Bsc4I	CCNNNNNNNGG	2 cut(s)	1189, 1463
Bse1I	ACTGG	2 cut(s)	847, 1457
BseBI	CCWGG	2 cut(s)	179, 614
BseDI	CCNNGG	8 cut(s)	177, 178, 303, 448, 562, 664, 1392, 1401
BseGI	GGATG	3 cut(s)	622, 963, 991
BseLI	CCNNNNNNNGG	2 cut(s)	1189, 1463
BseMII	CTCAG	6 cut(s)	209, 268, 357, 1245, 1397, 1481
BseNI	ACTGG	2 cut(s)	847, 1457
BseRI	GAGGAG	1 cut(s)	320
BseSI	GKGCMC	1 cut(s)	1224
BseXI	GCAGC	3 cut(s)	111, 302, 1023
BseYI	CCCAGC	1 cut(s)	1025
BsgI	GTGCAG	1 cut(s)	366
Bsh1236I	CGCG	3 cut(s)	288, 1090, 1336
Bsh1285I	CGRYCG	1 cut(s)	1121
BshFI	GGCC	2 cut(s)	1146, 1181
BsiEI	CGRYCG	1 cut(s)	1121
BsiHKAI	GWGCWC	1 cut(s)	1486
BslFI	GGGAC	5 cut(s)	122, 195, 328, 495, 754
BslI	CCNNNNNNNGG	2 cut(s)	1189, 1463
BsmAI	GTCTC	4 cut(s)	30, 544, 920, 1486
BsmFI	GGGAC	5 cut(s)	122, 195, 328, 495, 754
BsmI	GAATGC	1 cut(s)	980
BsnI	GGCC	2 cut(s)	1146, 1181
Bso31I	GGTCTC	1 cut(s)	1486
Bsp1286I	GDGCHC	4 cut(s)	255, 1224, 1360, 1486
BspACI	CCGC	6 cut(s)	20, 584, 795, 1009, 1030, 1336
BspANI	GGCC	2 cut(s)	1146, 1181
BspCNI	CTCAG	6 cut(s)	208, 267, 356, 1244, 1396, 1482
BspFNI	CGCG	3 cut(s)	288, 1090, 1336
BspLI	GGNNCC	6 cut(s)	175, 183, 265, 537, 1359, 1468
BspMI	ACCTGC	1 cut(s)	193
BspPI	GGATC	5 cut(s)	258, 271, 1000, 1351, 1448
BspTNI	GGTCTC	1 cut(s)	1486
BsrI	ACTGG	2 cut(s)	847, 1457
BssECI	CCNNGG	8 cut(s)	177, 178, 303, 448, 562, 664, 1392, 1401
BssT1I	CCWWGG	3 cut(s)	562, 664, 1392
Bst2UI	CCWGG	2 cut(s)	179, 614
Bst4CI	ACNGT	3 cut(s)	452, 829, 1501
BstAPI	GCANNNNNTGC	1 cut(s)	938
BstC8I	GCNNGC	6 cut(s)	286, 728, 878, 1290, 1389, 1518
BstDEI	CTNAG	7 cut(s)	195, 254, 343, 870, 1231, 1383, 1490
BstDSI	CCRYGG	2 cut(s)	448, 1401
BstF5I	GGATG	3 cut(s)	622, 963, 991
BstFNI	CGCG	3 cut(s)	288, 1090, 1336
BstHHI	GCGC	4 cut(s)	290, 1092, 1336, 1551
BstMAI	GTCTC	4 cut(s)	30, 544, 920, 1486
BstMCI	CGRYCG	1 cut(s)	1121
BstMWI	GCNNNNNNNGC	5 cut(s)	290, 732, 938, 1397, 1557
BstNI	CCWGG	2 cut(s)	179, 614
BstNSI	RCATGY	3 cut(s)	880, 1096, 1292
BstSCI	CCNGG	2 cut(s)	177, 612
BstSLI	GKGCMC	1 cut(s)	1224
BstUI	CGCG	3 cut(s)	288, 1090, 1336
BstV1I	GCAGC	3 cut(s)	111, 302, 1023
BstV2I	GAAGAC	1 cut(s)	1046
BstX2I	RGATCY	3 cut(s)	263, 1005, 1453
BstXI	CCANNNNNNTGG	1 cut(s)	640
BstYI	RGATCY	3 cut(s)	263, 1005, 1453
BsuRI	GGCC	2 cut(s)	1146, 1181
BtgI	CCRYGG	2 cut(s)	448, 1401
BtsCI	GGATG	3 cut(s)	622, 963, 991
BtsI	GCAGTG	1 cut(s)	1006
BtsIMutI	CAGTG	4 cut(s)	511, 1006, 1464, 1518
BveI	ACCTGC	1 cut(s)	193
Cac8I	GCNNGC	6 cut(s)	286, 728, 878, 1290, 1389, 1518
CaiI	CAGNNNCTG	1 cut(s)	1517
CfoI	GCGC	4 cut(s)	290, 1092, 1336, 1551
Cfr13I	GGNCC	5 cut(s)	182, 536, 1145, 1180, 1429
CseI	GACGC	1 cut(s)	1096
Csp6I	GTAC	1 cut(s)	1413
CviAII	CATG	8 cut(s)	418, 755, 877, 958, 1093, 1289, 1309, 1463
CviQI	GTAC	1 cut(s)	1413
DdeI	CTNAG	7 cut(s)	195, 254, 343, 870, 1231, 1383, 1490
DriI	GACNNNNNGTC	1 cut(s)	557
Eam1105I	GACNNNNNGTC	1 cut(s)	557
Eco130I	CCWWGG	3 cut(s)	562, 664, 1392
Eco24I	GRGCYC	2 cut(s)	255, 1360
Eco31I	GGTCTC	1 cut(s)	1486
Eco47I	GGWCC	3 cut(s)	182, 536, 1429
Eco57I	CTGAAG	2 cut(s)	240, 924
EcoO109I	RGGNCCY	3 cut(s)	182, 1145, 1429
EcoRII	CCWGG	2 cut(s)	177, 612
EcoT14I	CCWWGG	3 cut(s)	562, 664, 1392
EcoT22I	ATGCAT	1 cut(s)	1290
EcoT38I	GRGCYC	2 cut(s)	255, 1360
ErhI	CCWWGG	3 cut(s)	562, 664, 1392
FaeI	CATG	8 cut(s)	421, 758, 880, 961, 1096, 1292, 1312, 1466
FalI	AAGNNNNNCTT	2 cut(s)	1038, 1070
FaqI	GGGAC	5 cut(s)	122, 195, 328, 495, 754
FatI	CATG	8 cut(s)	417, 754, 876, 957, 1092, 1288, 1308, 1462
FauI	CCCGC	2 cut(s)	27, 577
FbaI	TGATCA	1 cut(s)	1081
Fnu4HI	GCNGC	4 cut(s)	125, 291, 1012, 1031
FokI	GGATG	3 cut(s)	629, 950, 998
FriOI	GRGCYC	2 cut(s)	255, 1360
Fsp4HI	GCNGC	4 cut(s)	125, 291, 1012, 1031
FspBI	CTAG	6 cut(s)	215, 461, 695, 1142, 1293, 1557
GlaI	GCGC	4 cut(s)	289, 1091, 1335, 1550
GluI	GCNGC	4 cut(s)	125, 291, 1012, 1031
GsaI	CCCAGC	1 cut(s)	1029
HaeIII	GGCC	2 cut(s)	1146, 1181
HgaI	GACGC	1 cut(s)	1096
HhaI	GCGC	4 cut(s)	290, 1092, 1336, 1551
Hin1II	CATG	8 cut(s)	421, 758, 880, 961, 1096, 1292, 1312, 1466
Hin6I	GCGC	4 cut(s)	288, 1090, 1334, 1549
HinP1I	GCGC	4 cut(s)	288, 1090, 1334, 1549
HinfI	GANTC	8 cut(s)	209, 377, 767, 809, 1123, 1259, 1268, 1276
HphI	GGTGA	4 cut(s)	292, 335, 1118, 1148
Hpy166II	GTNNAC	3 cut(s)	610, 1405, 1415
Hpy188III	TCNNGA	7 cut(s)	29, 80, 244, 256, 855, 925, 1427
Hpy8I	GTNNAC	3 cut(s)	610, 1405, 1415
Hpy99I	CGWCG	1 cut(s)	10
HpyAV	CCTTC	6 cut(s)	99, 253, 467, 910, 1072, 1210
HpyCH4III	ACNGT	3 cut(s)	452, 829, 1501
HpyCH4V	TGCA	8 cut(s)	383, 472, 605, 932, 957, 1288, 1462, 1526
HpyF10VI	GCNNNNNNNGC	5 cut(s)	290, 732, 938, 1397, 1557
HpyF3I	CTNAG	7 cut(s)	195, 254, 343, 870, 1231, 1383, 1490
Hsp92II	CATG	8 cut(s)	421, 758, 880, 961, 1096, 1292, 1312, 1466
HspAI	GCGC	4 cut(s)	288, 1090, 1334, 1549
Ksp22I	TGATCA	1 cut(s)	1081
LmnI	GCTCC	2 cut(s)	1363, 1466
Lsp1109I	GCAGC	3 cut(s)	111, 302, 1023
LweI	GCATC	3 cut(s)	121, 153, 1513
MaeI	CTAG	6 cut(s)	215, 461, 695, 1142, 1293, 1557
MaeIII	GTNAC	4 cut(s)	77, 685, 713, 838
MboII	GAAGA	6 cut(s)	73, 792, 818, 1046, 1087, 1225
MflI	RGATCY	3 cut(s)	263, 1005, 1453
MhlI	GDGCHC	4 cut(s)	255, 1224, 1360, 1486
MluCI	AATT	9 cut(s)	394, 510, 588, 592, 759, 846, 1254, 1318, 1561
MlyI	GAGTC	3 cut(s)	776, 1117, 1268
MmeI	TCCRAC	1 cut(s)	456
Mph1103I	ATGCAT	1 cut(s)	1290
MroXI	GAANNNNTTC	2 cut(s)	592, 920
MseI	TTAA	5 cut(s)	12, 276, 591, 1208, 1379
MslI	CAYNNNNRTG	2 cut(s)	615, 638
MspA1I	CMGCKG	5 cut(s)	22, 293, 795, 1133, 1166
MspR9I	CCNGG	2 cut(s)	179, 614
Mva1269I	GAATGC	1 cut(s)	980
MvaI	CCWGG	2 cut(s)	179, 614
MvnI	CGCG	3 cut(s)	288, 1090, 1336
MwoI	GCNNNNNNNGC	5 cut(s)	290, 732, 938, 1397, 1557
NlaIII	CATG	8 cut(s)	421, 758, 880, 961, 1096, 1292, 1312, 1466
NlaIV	GGNNCC	6 cut(s)	175, 183, 265, 537, 1359, 1468
NmuCI	GTSAC	3 cut(s)	77, 685, 713
NsiI	ATGCAT	1 cut(s)	1290
NspI	RCATGY	3 cut(s)	880, 1096, 1292
OliI	CACNNNNGTG	1 cut(s)	638
PaeI	GCATGC	2 cut(s)	880, 1292
PasI	CCCWGGG	1 cut(s)	178
PcsI	WCGNNNNNNNCGW	1 cut(s)	78
PctI	GAATGC	1 cut(s)	980
PdmI	GAANNNNTTC	2 cut(s)	592, 920
PfeI	GAWTC	5 cut(s)	209, 377, 809, 1268, 1276
PflMI	CCANNNNNTGG	1 cut(s)	1463
PkrI	GCNGC	4 cut(s)	126, 292, 1013, 1032
Ple19I	CGATCG	1 cut(s)	1121
PleI	GAGTC	3 cut(s)	775, 1117, 1267
PpsI	GAGTC	3 cut(s)	775, 1117, 1267
PpuMI	RGGWCCY	2 cut(s)	182, 1429
PshBI	ATTAAT	1 cut(s)	591
PsiI	TTATAA	1 cut(s)	819
Psp5II	RGGWCCY	2 cut(s)	182, 1429
Psp6I	CCWGG	2 cut(s)	177, 612
PspFI	CCCAGC	1 cut(s)	1025
PspGI	CCWGG	2 cut(s)	177, 612
PspN4I	GGNNCC	6 cut(s)	175, 183, 265, 537, 1359, 1468
PspPI	GGNCC	5 cut(s)	182, 536, 1145, 1180, 1429
PspPPI	RGGWCCY	2 cut(s)	182, 1429
PstNI	CAGNNNCTG	1 cut(s)	1517
PsuI	RGATCY	3 cut(s)	263, 1005, 1453
PvuI	CGATCG	1 cut(s)	1121
PvuII	CAGCTG	3 cut(s)	293, 1133, 1166
RsaI	GTAC	1 cut(s)	1414
RsaNI	GTAC	1 cut(s)	1413
RseI	CAYNNNNRTG	2 cut(s)	615, 638
SaqAI	TTAA	5 cut(s)	12, 276, 591, 1208, 1379
SatI	GCNGC	4 cut(s)	125, 291, 1012, 1031
Sau96I	GGNCC	5 cut(s)	182, 536, 1145, 1180, 1429
SchI	GAGTC	3 cut(s)	776, 1117, 1268
ScrFI	CCNGG	2 cut(s)	179, 614
SduI	GDGCHC	4 cut(s)	255, 1224, 1360, 1486
SfaNI	GCATC	3 cut(s)	121, 153, 1513
SinI	GGWCC	3 cut(s)	182, 536, 1429
SmiMI	CAYNNNNRTG	2 cut(s)	615, 638
SmlI	CTYRAG	2 cut(s)	189, 202
SmoI	CTYRAG	2 cut(s)	189, 202
SphI	GCATGC	2 cut(s)	880, 1292
Sse9I	AATT	9 cut(s)	394, 510, 588, 592, 759, 846, 1254, 1318, 1561
SsiI	CCGC	6 cut(s)	20, 584, 795, 1009, 1030, 1336
SspI	AATATT	2 cut(s)	235, 408
SspMI	CTAG	6 cut(s)	215, 461, 695, 1142, 1293, 1557
StyD4I	CCNGG	2 cut(s)	177, 612
StyI	CCWWGG	3 cut(s)	562, 664, 1392
TaaI	ACNGT	3 cut(s)	452, 829, 1501
TaqI	TCGA	4 cut(s)	36, 230, 924, 1121
TasI	AATT	9 cut(s)	394, 510, 588, 592, 759, 846, 1254, 1318, 1561
TauI	GCSGC	1 cut(s)	1033
TfiI	GAWTC	5 cut(s)	209, 377, 809, 1268, 1276
Tru1I	TTAA	5 cut(s)	12, 276, 591, 1208, 1379
Tru9I	TTAA	5 cut(s)	12, 276, 591, 1208, 1379
TscAI	CASTG	4 cut(s)	511, 1006, 1464, 1518
TseFI	GTSAC	3 cut(s)	77, 685, 713
TseI	GCWGC	3 cut(s)	124, 290, 1011
Tsp45I	GTSAC	3 cut(s)	77, 685, 713
TspDTI	ATGAA	7 cut(s)	261, 294, 434, 743, 987, 1260, 1297
TspGWI	ACGGA	1 cut(s)	528
TspRI	CASTG	4 cut(s)	511, 1006, 1464, 1518
Van91I	CCANNNNNTGG	1 cut(s)	1463
VpaK11BI	GGWCC	3 cut(s)	182, 536, 1429
VspI	ATTAAT	1 cut(s)	591
XceI	RCATGY	3 cut(s)	880, 1096, 1292
XcmI	CCANNNNNNNNNTGG	1 cut(s)	620
XmnI	GAANNNNTTC	2 cut(s)	592, 920
XspI	CTAG	6 cut(s)	215, 461, 695, 1142, 1293, 1557
Zsp2I	ATGCAT	1 cut(s)	1290

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.