RchiOBHm_Chr2g0129141

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009472
Physical Location & Seq
Forward (+)
44845304 .. 44850395
5092 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50083

Sequence Viewer

Length: 1992 bp
ATGAGTTGCGTCAATTGCCAAATTTTTTTTTTTTTGGTTCAACACTGGTTTTTACATTATTGGTTTGTTTTCTTCTGCCTTTCGAAGTCGTGTAAAGACACGTTAGCCACAAAAACCTACTATAAAAACCTGCTTAAACTCCAACGCCTACTCTCTGTTGCTCGCCGCCTCTCGTTGCCCTCTCACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCAGCTCGCACTAATGTCGGCTGCAACTTCCTCCTTCTCTCCAGCCCTCACCGCTACTCCACCTTCATCCTCCAAAGCCCACGCGGCGGCTCCCTCCACTCTCGCCTTCTCCTCCGCCGTCTCCAAGTCCCTCAACCCTCTCAGGCTCAGAGCCGCCTCTCGCTCTGCTGCTGTTGGCCGCGGATCCGCTCTCGGAGCTCGTATGGTCTCAATGCCGACGATTAAGCCCCCGCTCTCTCTTGATTTCGACACCTCTGTTTTCAACAAGGAGAAGGTCAACCTCGCTGGTCACGATGAGTATATTGTAAAGGGAGGAAGGGACTTGTTCCATCTGCTGCCTGATGCCTTCAAGGGCATCAAGCAGATCGGTGTCATCGGTTGGGGTTCCCAGGGACCTGCTCAAGCTCAGAACTTGAGAGATTCTCTAGCTGAAGCAAAGTCAAATATTGTGGTCAAGATTGGGCTCAGGAAGGGATCCCGTTCATTTAATGAAGCTCGCGCAGCTGGTTTCACCGAGGAGAATGGGACTTTAGGTGATATATGGGAAACAATCTCAGAGAGTGATCTTGTGTTGCTACTAATCTCTGATTCTGCACAGGCAGATAATTATGAGAAAATATTTTCGCACATGAAACCAAATAGCATACTTGGTCTTTCCCACGGTTTCCTTCTAGGGCATTTGCAGTCAGAGGGACTTGACTTTCCCAAGAACATCAGTGTAATTGCTGTATGCCCAAAGGGAATGGGTCCGTCAGTAAGGAGACTTTATGTCCAAGGCAAAGAAATAAATGGTGCGGGAATTAATTCAAGTTTTGCAGTCCACCAGGATGTTGATGGGAGAGCCACAGATGTGGCTCTGGGCTGGTCGGTTGCCCTTGGTTCTCCTTTCACATTTGTGACTACGCTAGAGCAGGAATACAAAAGTGACATCTTTGGCGAGCGAGGCATATTACTTGGTGCTGTTCATGGAATTGTGGAGTCCCTGTTCAGAAGATACACCGACAGCGGAATGAGTGAAGATTCTGCTTATAAAAATACAGTTGAATGTGTTACTGGAATTGTGTCAAGAACCATATCAACTAAGGGCATGTTGGAAATATACAGCTCTTTGTCTGAAGCTGACAGGAAGGAGTTCGAGACTGCATATAGTGCCTCCTATTATCCTTGCATGGACATCCTCTATGAATGCTATGAGGATGTAGCAAGCGGCAGTGAGATCCGCAGTGTTGTTTTGGCTGGGCGGCGATTTTATGAGAAAGATGGTCTTCCTGCCTTCCCTATGGGGAAGATTGATCAGACGCGCATGTGGAAAGTTGGTGAACGTGTCCGATCGACACGTCCAGCTGGTGATCTAGGCCCTCTACACCCTTTCACAGCTGGTGTTTTTGTGGCCCTGATGATGGCACAGATTGAAGTGTTAAGGAAGAAAGGGCACTCCTACTCAGAGATAATCAACGAAAGTGTAATCGAGTCGGTGGATTCTTTGAATCCTTTTATGCATGCTAGGGGAGTTTCTTTCATGGTTGATAATTGCTCAACAACAGCGCGGTTGGGATCAAGGAAATGGGCTCCAAGATTTGATTATATTCTAACTCAGCAAGCTTTGGTTGCCGTGGACAACGGTACACCCATCAATCAGGACCTCATTAGCAACTTCATGTCAGATCCAGTGCATGGAGCCATTGAAGTATGTGCTCAACTGAGACCCACAGTTGATATTTCAGTGCCTGCTGATGCAGACTTTGTGCGACCAGAGTTGCGCCAATCTAGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

663

Amino Acids

72.45

Weight (kDa)

7.96

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KARI_N PF07991 192 - 366 2.1e-31 Acetohydroxy acid isomeroreductase, NADPH-binding domain
KARI_C PF01450 375 - 519 8.9e-34 Ketol-acid reductoisomerase, C-terminal domain
KARI_C PF01450 531 - 600 5.4e-07 Ketol-acid reductoisomerase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1245
Acc36I ACCTGC 2 cut(s) 138, 619
AccB7I CCANNNNNTGG 1 cut(s) 1889
AccBSI CCGCTC 2 cut(s) 404, 448
AccII CGCG 5 cut(s) 299, 396, 714, 1516, 1762
AclWI GGATC 7 cut(s) 393, 406, 684, 697, 1426, 1777, 1874
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 1 cut(s) 21
AcuI CTGAAG 2 cut(s) 666, 1350
AfaI GTAC 1 cut(s) 1840
AfiI CCNNNNNNNGG 3 cut(s) 301, 1615, 1889
AflIII ACRYGT 3 cut(s) 99, 1537, 1550
AgsI TTSAA 8 cut(s) 41, 478, 565, 1023, 1259, 1628, 1702, 1901
AhdI GACNNNNNGTC 1 cut(s) 983
AjiI CACGTC 1 cut(s) 1553
AjnI CCWGG 2 cut(s) 603, 1038
AleI CACNNNNGTG 2 cut(s) 1064, 1109
AloI GAACNNNNNNTCC 2 cut(s) 1184, 1216
Alw21I GWGCWC 2 cut(s) 415, 1912
Alw26I GTCTC 5 cut(s) 340, 427, 970, 1346, 1912
AlwI GGATC 7 cut(s) 393, 406, 684, 697, 1426, 1777, 1874
AlwNI CAGNNNCTG 1 cut(s) 1943
AoxI GGCC 3 cut(s) 391, 1570, 1605
ApeKI GCWGC 4 cut(s) 236, 383, 550, 716
ApoI RAATTY 1 cut(s) 21
AseI ATTAAT 1 cut(s) 1017
Asp700I GAANNNNTTC 2 cut(s) 1018, 1346
AspLEI GCGC 4 cut(s) 716, 1518, 1762, 1977
AspS9I GGNCC 5 cut(s) 608, 962, 1571, 1606, 1855
AsuHPI GGTGA 5 cut(s) 256, 718, 761, 1544, 1574
AsuII TTCGAA 1 cut(s) 83
AvaII GGWCC 3 cut(s) 608, 962, 1855
BaeGI GKGCMC 1 cut(s) 1650
BamHI GGATCC 2 cut(s) 398, 689
BanII GRGCYC 3 cut(s) 415, 681, 1786
BbsI GAAGAC 1 cut(s) 1472
Bbv12I GWGCWC 2 cut(s) 415, 1912
BbvI GCAGC 4 cut(s) 223, 370, 537, 728
BccI CCATC 5 cut(s) 552, 1043, 1469, 1609, 1853
BceAI ACGGC 2 cut(s) 317, 1811
BcgI CGANNNNNNTGC 2 cut(s) 213, 247
BciT130I CCWGG 2 cut(s) 605, 1040
BclI TGATCA 1 cut(s) 1507
BcoDI GTCTC 5 cut(s) 340, 427, 970, 1346, 1912
BfaI CTAG 6 cut(s) 641, 887, 1121, 1568, 1719, 1983
BfuAI ACCTGC 2 cut(s) 138, 619
BglI GCCNNNNNGGC 1 cut(s) 299
Bme1390I CCNGG 2 cut(s) 605, 1040
Bme18I GGWCC 3 cut(s) 608, 962, 1855
BmeRI GACNNNNNGTC 1 cut(s) 983
BmgBI CACGTC 1 cut(s) 1553
BmgT120I GGNCC 5 cut(s) 608, 962, 1571, 1606, 1855
BmiI GGNNCC 8 cut(s) 306, 400, 601, 609, 691, 963, 1785, 1894
BmrFI CCNGG 2 cut(s) 605, 1040
BmsI GCATC 3 cut(s) 547, 579, 1939
BpiI GAAGAC 1 cut(s) 1472
BplI GAGNNNNNCTC 2 cut(s) 622, 654
BpmI CTGGAG 2 cut(s) 200, 240
Bpu10I CCTNAGC 1 cut(s) 680
Bpu14I TTCGAA 1 cut(s) 83
BpuEI CTTGAG 2 cut(s) 600, 649
BsaI GGTCTC 2 cut(s) 427, 1912
BsaJI CCNNGG 8 cut(s) 394, 603, 604, 729, 874, 988, 1090, 1827
BsaXI ACNNNNNCTCC 4 cut(s) 256, 286, 1184, 1214
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 1615, 1889
Bse1I ACTGG 3 cut(s) 50, 1273, 1883
BseBI CCWGG 2 cut(s) 605, 1040
BseDI CCNNGG 8 cut(s) 394, 603, 604, 729, 874, 988, 1090, 1827
BseGI GGATG 4 cut(s) 281, 1048, 1389, 1417
BseLI CCNNNNNNNGG 3 cut(s) 301, 1615, 1889
BseMII CTCAG 8 cut(s) 370, 376, 635, 694, 783, 1671, 1823, 1907
BseNI ACTGG 3 cut(s) 50, 1273, 1883
BseRI GAGGAG 2 cut(s) 316, 746
BseSI GKGCMC 1 cut(s) 1650
BseXI GCAGC 4 cut(s) 223, 370, 537, 728
BseYI CCCAGC 1 cut(s) 1451
BsgI GTGCAG 1 cut(s) 792
Bsh1236I CGCG 5 cut(s) 299, 396, 714, 1516, 1762
Bsh1285I CGRYCG 1 cut(s) 1547
BshFI GGCC 3 cut(s) 393, 1572, 1607
BsiEI CGRYCG 1 cut(s) 1547
BsiHKAI GWGCWC 2 cut(s) 415, 1912
BslFI GGGAC 6 cut(s) 328, 548, 621, 754, 921, 1180
BslI CCNNNNNNNGG 3 cut(s) 301, 1615, 1889
BsmAI GTCTC 5 cut(s) 340, 427, 970, 1346, 1912
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 6 cut(s) 328, 548, 621, 754, 921, 1180
BsmI GAATGC 1 cut(s) 1406
BsnI GGCC 3 cut(s) 393, 1572, 1607
Bso31I GGTCTC 2 cut(s) 427, 1912
Bsp119I TTCGAA 1 cut(s) 83
Bsp1286I GDGCHC 5 cut(s) 415, 681, 1650, 1786, 1912
BspANI GGCC 3 cut(s) 393, 1572, 1607
BspCNI CTCAG 8 cut(s) 369, 375, 634, 693, 782, 1670, 1822, 1908
BspFNI CGCG 5 cut(s) 299, 396, 714, 1516, 1762
BspLI GGNNCC 8 cut(s) 306, 400, 601, 609, 691, 963, 1785, 1894
BspMI ACCTGC 2 cut(s) 138, 619
BspPI GGATC 7 cut(s) 393, 406, 684, 697, 1426, 1777, 1874
BspT104I TTCGAA 1 cut(s) 83
BspTNI GGTCTC 2 cut(s) 427, 1912
BsrBI CCGCTC 2 cut(s) 404, 448
BsrI ACTGG 3 cut(s) 50, 1273, 1883
BssECI CCNNGG 8 cut(s) 394, 603, 604, 729, 874, 988, 1090, 1827
BssT1I CCWWGG 2 cut(s) 988, 1090
Bst2UI CCWGG 2 cut(s) 605, 1040
Bst4CI ACNGT 4 cut(s) 878, 1255, 1838, 1927
BstAPI GCANNNNNTGC 1 cut(s) 1364
BstBI TTCGAA 1 cut(s) 83
BstC8I GCNNGC 8 cut(s) 163, 222, 712, 1154, 1420, 1716, 1815, 1944
BstDEI CTNAG 9 cut(s) 356, 362, 621, 680, 769, 1296, 1657, 1809, 1916
BstDSI CCRYGG 3 cut(s) 394, 874, 1827
BstF5I GGATG 4 cut(s) 281, 1048, 1389, 1417
BstFNI CGCG 5 cut(s) 299, 396, 714, 1516, 1762
BstHHI GCGC 4 cut(s) 716, 1518, 1762, 1977
BstMAI GTCTC 5 cut(s) 340, 427, 970, 1346, 1912
BstMCI CGRYCG 1 cut(s) 1547
BstMWI GCNNNNNNNGC 9 cut(s) 15, 266, 299, 410, 716, 1158, 1364, 1823, 1983
BstNI CCWGG 2 cut(s) 605, 1040
BstNSI RCATGY 3 cut(s) 1306, 1522, 1718
BstSCI CCNGG 2 cut(s) 603, 1038
BstSLI GKGCMC 1 cut(s) 1650
BstUI CGCG 5 cut(s) 299, 396, 714, 1516, 1762
BstV1I GCAGC 4 cut(s) 223, 370, 537, 728
BstV2I GAAGAC 1 cut(s) 1472
BstX2I RGATCY 4 cut(s) 398, 689, 1431, 1879
BstXI CCANNNNNNTGG 1 cut(s) 1066
BstYI RGATCY 4 cut(s) 398, 689, 1431, 1879
BsuRI GGCC 3 cut(s) 393, 1572, 1607
BtgI CCRYGG 3 cut(s) 394, 874, 1827
BtrI CACGTC 1 cut(s) 1553
BtsCI GGATG 4 cut(s) 281, 1048, 1389, 1417
BtsI GCAGTG 2 cut(s) 1432, 1444
BtsIMutI CAGTG 6 cut(s) 43, 937, 1432, 1444, 1890, 1944
BveI ACCTGC 2 cut(s) 138, 619
Cac8I GCNNGC 8 cut(s) 163, 222, 712, 1154, 1420, 1716, 1815, 1944
CaiI CAGNNNCTG 1 cut(s) 1943
CfoI GCGC 4 cut(s) 716, 1518, 1762, 1977
Cfr13I GGNCC 5 cut(s) 608, 962, 1571, 1606, 1855
Cfr42I CCGCGG 1 cut(s) 397
CseI GACGC 1 cut(s) 1522
Csp6I GTAC 1 cut(s) 1839
CspCI CAANNNNNGTGG 2 cut(s) 645, 680
CviAII CATG 9 cut(s) 844, 1181, 1303, 1384, 1519, 1715, 1735, 1873, 1889
CviQI GTAC 1 cut(s) 1839
DdeI CTNAG 9 cut(s) 356, 362, 621, 680, 769, 1296, 1657, 1809, 1916
DriI GACNNNNNGTC 1 cut(s) 983
EaeI YGGCCR 1 cut(s) 391
Eam1105I GACNNNNNGTC 1 cut(s) 983
EciI GGCGGA 1 cut(s) 319
Ecl136II GAGCTC 1 cut(s) 413
Eco130I CCWWGG 2 cut(s) 988, 1090
Eco24I GRGCYC 3 cut(s) 415, 681, 1786
Eco31I GGTCTC 2 cut(s) 427, 1912
Eco47I GGWCC 3 cut(s) 608, 962, 1855
Eco53kI GAGCTC 1 cut(s) 413
Eco57I CTGAAG 2 cut(s) 666, 1350
EcoICRI GAGCTC 1 cut(s) 413
EcoO109I RGGNCCY 3 cut(s) 608, 1571, 1855
EcoRII CCWGG 2 cut(s) 603, 1038
EcoT14I CCWWGG 2 cut(s) 988, 1090
EcoT22I ATGCAT 1 cut(s) 1716
EcoT38I GRGCYC 3 cut(s) 415, 681, 1786
ErhI CCWWGG 2 cut(s) 988, 1090
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 9 cut(s) 847, 1184, 1306, 1387, 1522, 1718, 1738, 1876, 1892
FalI AAGNNNNNCTT 2 cut(s) 1464, 1496
FaqI GGGAC 6 cut(s) 328, 548, 621, 754, 921, 1180
FatI CATG 9 cut(s) 843, 1180, 1302, 1383, 1518, 1714, 1734, 1872, 1888
FauI CCCGC 2 cut(s) 453, 1003
FbaI TGATCA 1 cut(s) 1507
FokI GGATG 4 cut(s) 268, 1055, 1376, 1424
FriOI GRGCYC 3 cut(s) 415, 681, 1786
FspBI CTAG 6 cut(s) 641, 887, 1121, 1568, 1719, 1983
GlaI GCGC 4 cut(s) 715, 1517, 1761, 1976
GsaI CCCAGC 1 cut(s) 1455
GsuI CTGGAG 2 cut(s) 200, 240
HaeIII GGCC 3 cut(s) 393, 1572, 1607
HgaI GACGC 1 cut(s) 1522
HhaI GCGC 4 cut(s) 716, 1518, 1762, 1977
Hin1II CATG 9 cut(s) 847, 1184, 1306, 1387, 1522, 1718, 1738, 1876, 1892
Hin6I GCGC 4 cut(s) 714, 1516, 1760, 1975
HinP1I GCGC 4 cut(s) 714, 1516, 1760, 1975
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HindIII AAGCTT 1 cut(s) 1815
HinfI GANTC 7 cut(s) 635, 803, 1193, 1235, 1685, 1694, 1702
HphI GGTGA 5 cut(s) 256, 718, 761, 1544, 1574
Hpy166II GTNNAC 5 cut(s) 493, 1036, 1535, 1831, 1841
Hpy188III TCNNGA 7 cut(s) 455, 506, 670, 682, 1281, 1351, 1853
Hpy8I GTNNAC 5 cut(s) 493, 1036, 1535, 1831, 1841
Hpy99I CGWCG 1 cut(s) 436
HpyCH4III ACNGT 4 cut(s) 878, 1255, 1838, 1927
HpyCH4IV ACGT 3 cut(s) 101, 1537, 1552
HpyCH4V TGCA 9 cut(s) 239, 809, 898, 1031, 1358, 1383, 1714, 1888, 1952
HpyF10VI GCNNNNNNNGC 9 cut(s) 15, 266, 299, 410, 716, 1158, 1364, 1823, 1983
HpyF3I CTNAG 9 cut(s) 356, 362, 621, 680, 769, 1296, 1657, 1809, 1916
HpySE526I ACGT 3 cut(s) 101, 1537, 1552
Hsp92II CATG 9 cut(s) 847, 1184, 1306, 1387, 1522, 1718, 1738, 1876, 1892
HspAI GCGC 4 cut(s) 714, 1516, 1760, 1975
Ksp22I TGATCA 1 cut(s) 1507
KspI CCGCGG 1 cut(s) 397
LmnI GCTCC 4 cut(s) 310, 410, 1789, 1892
Lsp1109I GCAGC 4 cut(s) 223, 370, 537, 728
LweI GCATC 3 cut(s) 547, 579, 1939
MaeI CTAG 6 cut(s) 641, 887, 1121, 1568, 1719, 1983
MaeII ACGT 3 cut(s) 101, 1537, 1552
MaeIII GTNAC 4 cut(s) 503, 1111, 1139, 1264
MbiI CCGCTC 2 cut(s) 404, 448
MboII GAAGA 6 cut(s) 64, 1218, 1244, 1472, 1513, 1651
MfeI CAATTG 1 cut(s) 13
MflI RGATCY 4 cut(s) 398, 689, 1431, 1879
MhlI GDGCHC 5 cut(s) 415, 681, 1650, 1786, 1912
MlyI GAGTC 2 cut(s) 1202, 1694
MmeI TCCRAC 2 cut(s) 166, 1287
Mph1103I ATGCAT 1 cut(s) 1716
MroXI GAANNNNTTC 2 cut(s) 1018, 1346
MseI TTAA 5 cut(s) 135, 438, 702, 1017, 1634
MslI CAYNNNNRTG 3 cut(s) 1041, 1064, 1109
MspA1I CMGCKG 5 cut(s) 396, 719, 1221, 1559, 1592
MspR9I CCNGG 2 cut(s) 605, 1040
MunI CAATTG 1 cut(s) 13
Mva1269I GAATGC 1 cut(s) 1406
MvaI CCWGG 2 cut(s) 605, 1040
MvnI CGCG 5 cut(s) 299, 396, 714, 1516, 1762
MwoI GCNNNNNNNGC 9 cut(s) 15, 266, 299, 410, 716, 1158, 1364, 1823, 1983
NlaIII CATG 9 cut(s) 847, 1184, 1306, 1387, 1522, 1718, 1738, 1876, 1892
NlaIV GGNNCC 8 cut(s) 306, 400, 601, 609, 691, 963, 1785, 1894
NmuCI GTSAC 3 cut(s) 503, 1111, 1139
NsiI ATGCAT 1 cut(s) 1716
NspI RCATGY 3 cut(s) 1306, 1522, 1718
NspV TTCGAA 1 cut(s) 83
OliI CACNNNNGTG 2 cut(s) 1064, 1109
PaeI GCATGC 1 cut(s) 1718
PasI CCCWGGG 1 cut(s) 604
PcsI WCGNNNNNNNCGW 1 cut(s) 504
PctI GAATGC 1 cut(s) 1406
PdmI GAANNNNTTC 2 cut(s) 1018, 1346
PfeI GAWTC 5 cut(s) 635, 803, 1235, 1694, 1702
PflMI CCANNNNNTGG 1 cut(s) 1889
Ple19I CGATCG 1 cut(s) 1547
PleI GAGTC 2 cut(s) 1201, 1693
PpsI GAGTC 2 cut(s) 1201, 1693
PpuMI RGGWCCY 2 cut(s) 608, 1855
PshBI ATTAAT 1 cut(s) 1017
PsiI TTATAA 1 cut(s) 1245
Psp124BI GAGCTC 1 cut(s) 415
Psp5II RGGWCCY 2 cut(s) 608, 1855
Psp6I CCWGG 2 cut(s) 603, 1038
PspFI CCCAGC 1 cut(s) 1451
PspGI CCWGG 2 cut(s) 603, 1038
PspN4I GGNNCC 8 cut(s) 306, 400, 601, 609, 691, 963, 1785, 1894
PspPI GGNCC 5 cut(s) 608, 962, 1571, 1606, 1855
PspPPI RGGWCCY 2 cut(s) 608, 1855
PstNI CAGNNNCTG 1 cut(s) 1943
PsuI RGATCY 4 cut(s) 398, 689, 1431, 1879
PvuI CGATCG 1 cut(s) 1547
PvuII CAGCTG 3 cut(s) 719, 1559, 1592
RsaI GTAC 1 cut(s) 1840
RsaNI GTAC 1 cut(s) 1839
RseI CAYNNNNRTG 3 cut(s) 1041, 1064, 1109
SacI GAGCTC 1 cut(s) 415
SacII CCGCGG 1 cut(s) 397
SaqAI TTAA 5 cut(s) 135, 438, 702, 1017, 1634
Sau96I GGNCC 5 cut(s) 608, 962, 1571, 1606, 1855
SchI GAGTC 2 cut(s) 1202, 1694
ScrFI CCNGG 2 cut(s) 605, 1040
SduI GDGCHC 5 cut(s) 415, 681, 1650, 1786, 1912
SfaNI GCATC 3 cut(s) 547, 579, 1939
Sfr303I CCGCGG 1 cut(s) 397
SfuI TTCGAA 1 cut(s) 83
SgrBI CCGCGG 1 cut(s) 397
SinI GGWCC 3 cut(s) 608, 962, 1855
SmiMI CAYNNNNRTG 3 cut(s) 1041, 1064, 1109
SmlI CTYRAG 2 cut(s) 615, 628
SmoI CTYRAG 2 cut(s) 615, 628
SphI GCATGC 1 cut(s) 1718
SspI AATATT 2 cut(s) 661, 834
SspMI CTAG 6 cut(s) 641, 887, 1121, 1568, 1719, 1983
SstI GAGCTC 1 cut(s) 415
StyD4I CCNGG 2 cut(s) 603, 1038
StyI CCWWGG 2 cut(s) 988, 1090
TaaI ACNGT 4 cut(s) 878, 1255, 1838, 1927
TaiI ACGT 3 cut(s) 104, 1540, 1555
TaqI TCGA 5 cut(s) 83, 462, 1350, 1547, 1683
TauI GCSGC 7 cut(s) 168, 302, 305, 371, 396, 1425, 1459
TfiI GAWTC 5 cut(s) 635, 803, 1235, 1694, 1702
Tru1I TTAA 5 cut(s) 135, 438, 702, 1017, 1634
Tru9I TTAA 5 cut(s) 135, 438, 702, 1017, 1634
TscAI CASTG 6 cut(s) 50, 937, 1432, 1444, 1890, 1944
TseFI GTSAC 3 cut(s) 503, 1111, 1139
TseI GCWGC 4 cut(s) 236, 383, 550, 716
Tsp45I GTSAC 3 cut(s) 503, 1111, 1139
TspDTI ATGAA 8 cut(s) 270, 687, 720, 860, 1169, 1413, 1723, 1861
TspGWI ACGGA 1 cut(s) 954
TspRI CASTG 6 cut(s) 50, 937, 1432, 1444, 1890, 1944
Van91I CCANNNNNTGG 1 cut(s) 1889
VpaK11BI GGWCC 3 cut(s) 608, 962, 1855
VspI ATTAAT 1 cut(s) 1017
XapI RAATTY 1 cut(s) 21
XceI RCATGY 3 cut(s) 1306, 1522, 1718
XcmI CCANNNNNNNNNTGG 1 cut(s) 1046
XmnI GAANNNNTTC 2 cut(s) 1018, 1346
XspI CTAG 6 cut(s) 641, 887, 1121, 1568, 1719, 1983
Zsp2I ATGCAT 1 cut(s) 1716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.