Rh2DG362000

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009472
Physical Location & Seq
Forward (+)
51176714 .. 51181602
4889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG362000.1

Sequence Viewer

Length: 1764 bp
ATGTCGGCTGCAACTTCCTCCTTCTCTCCAGCCCTCACCGCTACTCCACCTTCATCCTCCAAAGCCCACGCGGCGGCTCCCTCCACGCTCGCCTTCTCCTCCGCCGTCTCCAAGTCCCTCAACCCTCTCAGGCTCAGAGCCGCCTCTCGCGCTGCTGCTGATGGCCGCGGATCCGCTCTCGGAGCTCGTATGGTCTCAATGCCGACGATTAAGCCCCCGCTCTCTCTTGATTTCGACACCTCTGTTTTCAACAAGGAGAAGGTCAACCTCGCTGGTCACGATGAGTATATTGTAAAGGGAGGAAGGGACTTGTTCCATCTGCTGCCTGATGCCTTCAAGGGCATCAAGCAGATCGGTGTCATCGGTTGGGGTTCCCAGGGACCTGCTCAAGCTCAGAACTTGAGAGATTCTCTAGCTGAAGCAAAGTCAAATATTGTGGTCAAGATTGGGCTCAGGAAGGGATCCCGTTCATTTAATGAAGCTCGCGCAGCTGGTTTCACTGAGGAGAATGGGACTTTAGGTGATATATGGGAAACAATCTCAGAGAGTGATCTTGTGTTGCTACTAATCTCTGATTCTGCACAGGCAGATAATTATGAGAAAATATTTTCGCACATGAAACCAAATAGCATACTTGGTCTTTCCCACGGTTTCCTTCTAGGGCATTTGCAGTCGGAGGGACTTGACTTTCCCAAGAACATCAGTGTAATTGCTGTATGCCCAAAGGGAATGGGTCCGTCAGTAAGGAGACTTTATGTCCAAGGCAAAGAAATAAATGGTGCGGGAATTAATTCAAGTTTTGCAGTCCACCAGGATGTTGATGGGAGAGCCACAGATGTGGCTCTGGGCTGGTCGGTTGCCCTTGGTTCTCCTTTCACATTTGTTACTACGCTAGAGCAGGAATACAAAAGTGACATCTTTGGCGAGCGAGGCATATTACTTGGTGCTGTTCATGGAATTGTGGAGTCCCTGTTCAGAAGATACACCGACAGCGGAATGAGTGAAGATTCTGCTTATAAAAATACCGTTGAATGTGTTACTGGAATTGTGTCAAGAACCATATCAACTAAGGGCATGTTGGAAATATACAGCTCTTTGTCTGAAGCTGACAGGAAGGAGTTCGAGACTGCATATAGTGCTTCCTATTATCCTTGCATGGACATCCTCTATGAATGCTATGAGGATGTAGCAAGCGGCAGTGAGATCCGCAGCGTTGTTTTGGCTGGGCGGCGATTTTATGAGAAAGATGGTCTTCCTGCCTTCCCTATGGGGAAGATTGATCAGACGCGCATGTGGAAAGTTGGTGAACGTGTCCGATCGACACGTCCAGCTGGTGATCTAGGCCCTCTACACCCTTTTACAGCTGGTGTTTTTGTGGCCCTGATGATGGCACAGATTGAAGTGTTAAGGAAGAAAGGGCACTCCTACTCAGAGATAATCAACGAAAGTGTAATTGAGTCGGTGGATTCTTTGAATCCTTTTATGCATGCTAGGGGAGTTTCTTTCATGGTTGATAATTGCTCAACAACAGCGCGGTTGGGATCAAGGAAATGGGCTCCAAGATTTGATTATATTCTAACTCAGCAAGCTTTGGTTTCCGTGGACAACGGTACACCCATCAATCAGGACCTCATTAGCAACTTCATGTCAGATCCAGTGCATGGAGCCATTGAAGTATGTGCTCAACTGAGACCCACAGTTGATATTTCAGTGCCTGCTGATGCAGACTTTGTGCGACCAGAGTTGCGCCAATCTAGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

587

Amino Acids

63.53

Weight (kDa)

6.11

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KARI_N PF07991 116 - 290 1.7e-31 Acetohydroxy acid isomeroreductase, NADPH-binding domain
KARI_C PF01450 299 - 443 7.2e-34 Ketol-acid reductoisomerase, C-terminal domain
KARI_C PF01450 455 - 525 4.1e-07 Ketol-acid reductoisomerase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
Acc36I ACCTGC 1 cut(s) 391
AccB7I CCANNNNNTGG 1 cut(s) 1661
AccBSI CCGCTC 2 cut(s) 176, 220
AccII CGCG 6 cut(s) 71, 150, 168, 486, 1288, 1534
AclWI GGATC 7 cut(s) 165, 178, 456, 469, 1198, 1549, 1646
AcoI YGGCCR 1 cut(s) 163
AcuI CTGAAG 2 cut(s) 438, 1122
AfaI GTAC 1 cut(s) 1612
AfiI CCNNNNNNNGG 3 cut(s) 73, 1387, 1661
AflIII ACRYGT 2 cut(s) 1309, 1322
AgsI TTSAA 7 cut(s) 250, 337, 795, 1031, 1400, 1474, 1673
AhdI GACNNNNNGTC 1 cut(s) 755
AjiI CACGTC 1 cut(s) 1325
AjnI CCWGG 2 cut(s) 375, 810
AleI CACNNNNGTG 1 cut(s) 836
AloI GAACNNNNNNTCC 2 cut(s) 956, 988
Alw21I GWGCWC 2 cut(s) 187, 1684
Alw26I GTCTC 5 cut(s) 112, 199, 742, 1118, 1684
AlwI GGATC 7 cut(s) 165, 178, 456, 469, 1198, 1549, 1646
AlwNI CAGNNNCTG 1 cut(s) 1715
AoxI GGCC 3 cut(s) 163, 1342, 1377
ApeKI GCWGC 6 cut(s) 8, 152, 155, 322, 488, 1209
AseI ATTAAT 1 cut(s) 789
Asp700I GAANNNNTTC 2 cut(s) 790, 1118
AspLEI GCGC 5 cut(s) 152, 488, 1290, 1534, 1749
AspS9I GGNCC 5 cut(s) 380, 734, 1343, 1378, 1627
AsuHPI GGTGA 4 cut(s) 28, 533, 1316, 1346
AvaII GGWCC 3 cut(s) 380, 734, 1627
BaeGI GKGCMC 1 cut(s) 1422
BamHI GGATCC 2 cut(s) 170, 461
BanII GRGCYC 3 cut(s) 187, 453, 1558
BbsI GAAGAC 1 cut(s) 1244
Bbv12I GWGCWC 2 cut(s) 187, 1684
BbvI GCAGC 5 cut(s) 139, 142, 309, 500, 1221
BccI CCATC 6 cut(s) 155, 324, 815, 1241, 1381, 1625
BceAI ACGGC 1 cut(s) 89
BciT130I CCWGG 2 cut(s) 377, 812
BclI TGATCA 1 cut(s) 1279
BcoDI GTCTC 5 cut(s) 112, 199, 742, 1118, 1684
BfaI CTAG 6 cut(s) 413, 659, 893, 1340, 1491, 1755
BfuAI ACCTGC 1 cut(s) 391
BglI GCCNNNNNGGC 1 cut(s) 71
Bme1390I CCNGG 2 cut(s) 377, 812
Bme18I GGWCC 3 cut(s) 380, 734, 1627
BmeRI GACNNNNNGTC 1 cut(s) 755
BmgBI CACGTC 1 cut(s) 1325
BmgT120I GGNCC 5 cut(s) 380, 734, 1343, 1378, 1627
BmiI GGNNCC 8 cut(s) 78, 172, 373, 381, 463, 735, 1557, 1666
BmrFI CCNGG 2 cut(s) 377, 812
BmsI GCATC 3 cut(s) 319, 351, 1711
BpiI GAAGAC 1 cut(s) 1244
BplI GAGNNNNNCTC 2 cut(s) 394, 426
BpmI CTGGAG 1 cut(s) 12
Bpu10I CCTNAGC 1 cut(s) 452
BpuEI CTTGAG 2 cut(s) 372, 421
BsaI GGTCTC 2 cut(s) 199, 1684
BsaJI CCNNGG 7 cut(s) 166, 375, 376, 646, 760, 862, 1599
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 956, 986
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 1387, 1661
Bse1I ACTGG 2 cut(s) 1045, 1655
BseBI CCWGG 2 cut(s) 377, 812
BseDI CCNNGG 7 cut(s) 166, 375, 376, 646, 760, 862, 1599
BseGI GGATG 4 cut(s) 53, 820, 1161, 1189
BseLI CCNNNNNNNGG 3 cut(s) 73, 1387, 1661
BseMII CTCAG 9 cut(s) 142, 148, 407, 466, 492, 555, 1443, 1595, 1679
BseNI ACTGG 2 cut(s) 1045, 1655
BseRI GAGGAG 2 cut(s) 88, 518
BseSI GKGCMC 1 cut(s) 1422
BseXI GCAGC 5 cut(s) 139, 142, 309, 500, 1221
BseYI CCCAGC 1 cut(s) 1223
BsgI GTGCAG 1 cut(s) 564
Bsh1236I CGCG 6 cut(s) 71, 150, 168, 486, 1288, 1534
Bsh1285I CGRYCG 1 cut(s) 1319
BshFI GGCC 3 cut(s) 165, 1344, 1379
BsiEI CGRYCG 1 cut(s) 1319
BsiHKAI GWGCWC 2 cut(s) 187, 1684
BslFI GGGAC 6 cut(s) 100, 320, 393, 526, 693, 952
BslI CCNNNNNNNGG 3 cut(s) 73, 1387, 1661
BsmAI GTCTC 5 cut(s) 112, 199, 742, 1118, 1684
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 6 cut(s) 100, 320, 393, 526, 693, 952
BsmI GAATGC 1 cut(s) 1178
BsnI GGCC 3 cut(s) 165, 1344, 1379
Bso31I GGTCTC 2 cut(s) 199, 1684
Bsp1286I GDGCHC 5 cut(s) 187, 453, 1422, 1558, 1684
BspANI GGCC 3 cut(s) 165, 1344, 1379
BspCNI CTCAG 9 cut(s) 141, 147, 406, 465, 493, 554, 1442, 1594, 1680
BspFNI CGCG 6 cut(s) 71, 150, 168, 486, 1288, 1534
BspLI GGNNCC 8 cut(s) 78, 172, 373, 381, 463, 735, 1557, 1666
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 7 cut(s) 165, 178, 456, 469, 1198, 1549, 1646
BspTNI GGTCTC 2 cut(s) 199, 1684
BsrBI CCGCTC 2 cut(s) 176, 220
BsrI ACTGG 2 cut(s) 1045, 1655
BssECI CCNNGG 7 cut(s) 166, 375, 376, 646, 760, 862, 1599
BssT1I CCWWGG 2 cut(s) 760, 862
Bst2UI CCWGG 2 cut(s) 377, 812
Bst4CI ACNGT 4 cut(s) 650, 1027, 1610, 1699
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstC8I GCNNGC 7 cut(s) 90, 484, 926, 1192, 1488, 1587, 1716
BstDSI CCRYGG 3 cut(s) 166, 646, 1599
BstF5I GGATG 4 cut(s) 53, 820, 1161, 1189
BstFNI CGCG 6 cut(s) 71, 150, 168, 486, 1288, 1534
BstHHI GCGC 5 cut(s) 152, 488, 1290, 1534, 1749
BstMAI GTCTC 5 cut(s) 112, 199, 742, 1118, 1684
BstMCI CGRYCG 1 cut(s) 1319
BstMWI GCNNNNNNNGC 8 cut(s) 38, 71, 149, 182, 488, 930, 1136, 1755
BstNI CCWGG 2 cut(s) 377, 812
BstNSI RCATGY 3 cut(s) 1078, 1294, 1490
BstSCI CCNGG 2 cut(s) 375, 810
BstSLI GKGCMC 1 cut(s) 1422
BstUI CGCG 6 cut(s) 71, 150, 168, 486, 1288, 1534
BstV1I GCAGC 5 cut(s) 139, 142, 309, 500, 1221
BstV2I GAAGAC 1 cut(s) 1244
BstX2I RGATCY 4 cut(s) 170, 461, 1203, 1651
BstXI CCANNNNNNTGG 1 cut(s) 838
BstYI RGATCY 4 cut(s) 170, 461, 1203, 1651
BsuRI GGCC 3 cut(s) 165, 1344, 1379
BtgI CCRYGG 3 cut(s) 166, 646, 1599
BtrI CACGTC 1 cut(s) 1325
BtsCI GGATG 4 cut(s) 53, 820, 1161, 1189
BtsI GCAGTG 1 cut(s) 1204
BtsIMutI CAGTG 5 cut(s) 498, 709, 1204, 1662, 1716
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 7 cut(s) 90, 484, 926, 1192, 1488, 1587, 1716
CaiI CAGNNNCTG 1 cut(s) 1715
CfoI GCGC 5 cut(s) 152, 488, 1290, 1534, 1749
Cfr13I GGNCC 5 cut(s) 380, 734, 1343, 1378, 1627
Cfr42I CCGCGG 1 cut(s) 169
CseI GACGC 1 cut(s) 1294
Csp6I GTAC 1 cut(s) 1611
CspCI CAANNNNNGTGG 2 cut(s) 417, 452
CviAII CATG 9 cut(s) 616, 953, 1075, 1156, 1291, 1487, 1507, 1645, 1661
CviQI GTAC 1 cut(s) 1611
DriI GACNNNNNGTC 1 cut(s) 755
EaeI YGGCCR 1 cut(s) 163
Eam1105I GACNNNNNGTC 1 cut(s) 755
EciI GGCGGA 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 185
Eco130I CCWWGG 2 cut(s) 760, 862
Eco24I GRGCYC 3 cut(s) 187, 453, 1558
Eco31I GGTCTC 2 cut(s) 199, 1684
Eco47I GGWCC 3 cut(s) 380, 734, 1627
Eco53kI GAGCTC 1 cut(s) 185
Eco57I CTGAAG 2 cut(s) 438, 1122
EcoICRI GAGCTC 1 cut(s) 185
EcoO109I RGGNCCY 3 cut(s) 380, 1343, 1627
EcoRII CCWGG 2 cut(s) 375, 810
EcoT14I CCWWGG 2 cut(s) 760, 862
EcoT22I ATGCAT 1 cut(s) 1488
EcoT38I GRGCYC 3 cut(s) 187, 453, 1558
ErhI CCWWGG 2 cut(s) 760, 862
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 9 cut(s) 619, 956, 1078, 1159, 1294, 1490, 1510, 1648, 1664
FalI AAGNNNNNCTT 2 cut(s) 1236, 1268
FaqI GGGAC 6 cut(s) 100, 320, 393, 526, 693, 952
FatI CATG 9 cut(s) 615, 952, 1074, 1155, 1290, 1486, 1506, 1644, 1660
FauI CCCGC 2 cut(s) 225, 775
FbaI TGATCA 1 cut(s) 1279
FokI GGATG 4 cut(s) 40, 827, 1148, 1196
FriOI GRGCYC 3 cut(s) 187, 453, 1558
FspBI CTAG 6 cut(s) 413, 659, 893, 1340, 1491, 1755
GlaI GCGC 5 cut(s) 151, 487, 1289, 1533, 1748
GsaI CCCAGC 1 cut(s) 1227
GsuI CTGGAG 1 cut(s) 12
HaeIII GGCC 3 cut(s) 165, 1344, 1379
HgaI GACGC 1 cut(s) 1294
HhaI GCGC 5 cut(s) 152, 488, 1290, 1534, 1749
Hin1II CATG 9 cut(s) 619, 956, 1078, 1159, 1294, 1490, 1510, 1648, 1664
Hin6I GCGC 5 cut(s) 150, 486, 1288, 1532, 1747
HinP1I GCGC 5 cut(s) 150, 486, 1288, 1532, 1747
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 1587
HinfI GANTC 7 cut(s) 407, 575, 965, 1007, 1457, 1466, 1474
HphI GGTGA 4 cut(s) 28, 533, 1316, 1346
Hpy166II GTNNAC 5 cut(s) 265, 808, 1307, 1603, 1613
Hpy188III TCNNGA 7 cut(s) 227, 278, 442, 454, 1053, 1123, 1625
Hpy8I GTNNAC 5 cut(s) 265, 808, 1307, 1603, 1613
Hpy99I CGWCG 1 cut(s) 208
HpyCH4III ACNGT 4 cut(s) 650, 1027, 1610, 1699
HpyCH4IV ACGT 2 cut(s) 1309, 1324
HpyCH4V TGCA 9 cut(s) 11, 581, 670, 803, 1130, 1155, 1486, 1660, 1724
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 71, 149, 182, 488, 930, 1136, 1755
HpySE526I ACGT 2 cut(s) 1309, 1324
Hsp92II CATG 9 cut(s) 619, 956, 1078, 1159, 1294, 1490, 1510, 1648, 1664
HspAI GCGC 5 cut(s) 150, 486, 1288, 1532, 1747
Ksp22I TGATCA 1 cut(s) 1279
KspI CCGCGG 1 cut(s) 169
LmnI GCTCC 4 cut(s) 82, 182, 1561, 1664
Lsp1109I GCAGC 5 cut(s) 139, 142, 309, 500, 1221
LweI GCATC 3 cut(s) 319, 351, 1711
MaeI CTAG 6 cut(s) 413, 659, 893, 1340, 1491, 1755
MaeII ACGT 2 cut(s) 1309, 1324
MaeIII GTNAC 4 cut(s) 275, 883, 911, 1036
MbiI CCGCTC 2 cut(s) 176, 220
MboII GAAGA 5 cut(s) 990, 1016, 1244, 1285, 1423
MflI RGATCY 4 cut(s) 170, 461, 1203, 1651
MhlI GDGCHC 5 cut(s) 187, 453, 1422, 1558, 1684
MluCI AATT 9 cut(s) 592, 708, 786, 790, 957, 1044, 1452, 1516, 1759
MlyI GAGTC 2 cut(s) 974, 1466
MmeI TCCRAC 2 cut(s) 654, 1059
Mph1103I ATGCAT 1 cut(s) 1488
MroXI GAANNNNTTC 2 cut(s) 790, 1118
MseI TTAA 4 cut(s) 210, 474, 789, 1406
MslI CAYNNNNRTG 2 cut(s) 813, 836
MspA1I CMGCKG 5 cut(s) 168, 491, 993, 1331, 1364
MspR9I CCNGG 2 cut(s) 377, 812
Mva1269I GAATGC 1 cut(s) 1178
MvaI CCWGG 2 cut(s) 377, 812
MvnI CGCG 6 cut(s) 71, 150, 168, 486, 1288, 1534
MwoI GCNNNNNNNGC 8 cut(s) 38, 71, 149, 182, 488, 930, 1136, 1755
NlaIII CATG 9 cut(s) 619, 956, 1078, 1159, 1294, 1490, 1510, 1648, 1664
NlaIV GGNNCC 8 cut(s) 78, 172, 373, 381, 463, 735, 1557, 1666
NmuCI GTSAC 2 cut(s) 275, 911
NsiI ATGCAT 1 cut(s) 1488
NspI RCATGY 3 cut(s) 1078, 1294, 1490
OliI CACNNNNGTG 1 cut(s) 836
PaeI GCATGC 1 cut(s) 1490
PasI CCCWGGG 1 cut(s) 376
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 1178
PdmI GAANNNNTTC 2 cut(s) 790, 1118
PfeI GAWTC 5 cut(s) 407, 575, 1007, 1466, 1474
PflMI CCANNNNNTGG 1 cut(s) 1661
Ple19I CGATCG 1 cut(s) 1319
PleI GAGTC 2 cut(s) 973, 1465
PpsI GAGTC 2 cut(s) 973, 1465
PpuMI RGGWCCY 2 cut(s) 380, 1627
PshBI ATTAAT 1 cut(s) 789
PsiI TTATAA 1 cut(s) 1017
Psp124BI GAGCTC 1 cut(s) 187
Psp5II RGGWCCY 2 cut(s) 380, 1627
Psp6I CCWGG 2 cut(s) 375, 810
PspFI CCCAGC 1 cut(s) 1223
PspGI CCWGG 2 cut(s) 375, 810
PspN4I GGNNCC 8 cut(s) 78, 172, 373, 381, 463, 735, 1557, 1666
PspPI GGNCC 5 cut(s) 380, 734, 1343, 1378, 1627
PspPPI RGGWCCY 2 cut(s) 380, 1627
PstNI CAGNNNCTG 1 cut(s) 1715
PsuI RGATCY 4 cut(s) 170, 461, 1203, 1651
PvuI CGATCG 1 cut(s) 1319
PvuII CAGCTG 3 cut(s) 491, 1331, 1364
RsaI GTAC 1 cut(s) 1612
RsaNI GTAC 1 cut(s) 1611
RseI CAYNNNNRTG 2 cut(s) 813, 836
SacI GAGCTC 1 cut(s) 187
SacII CCGCGG 1 cut(s) 169
SaqAI TTAA 4 cut(s) 210, 474, 789, 1406
Sau96I GGNCC 5 cut(s) 380, 734, 1343, 1378, 1627
SchI GAGTC 2 cut(s) 974, 1466
ScrFI CCNGG 2 cut(s) 377, 812
SduI GDGCHC 5 cut(s) 187, 453, 1422, 1558, 1684
SfaNI GCATC 3 cut(s) 319, 351, 1711
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 3 cut(s) 380, 734, 1627
SmiMI CAYNNNNRTG 2 cut(s) 813, 836
SmlI CTYRAG 2 cut(s) 387, 400
SmoI CTYRAG 2 cut(s) 387, 400
SphI GCATGC 1 cut(s) 1490
Sse9I AATT 9 cut(s) 592, 708, 786, 790, 957, 1044, 1452, 1516, 1759
SspI AATATT 2 cut(s) 433, 606
SspMI CTAG 6 cut(s) 413, 659, 893, 1340, 1491, 1755
SstI GAGCTC 1 cut(s) 187
StyD4I CCNGG 2 cut(s) 375, 810
StyI CCWWGG 2 cut(s) 760, 862
TaaI ACNGT 4 cut(s) 650, 1027, 1610, 1699
TaiI ACGT 2 cut(s) 1312, 1327
TaqI TCGA 3 cut(s) 234, 1122, 1319
TasI AATT 9 cut(s) 592, 708, 786, 790, 957, 1044, 1452, 1516, 1759
TauI GCSGC 6 cut(s) 74, 77, 143, 168, 1197, 1231
TfiI GAWTC 5 cut(s) 407, 575, 1007, 1466, 1474
Tru1I TTAA 4 cut(s) 210, 474, 789, 1406
Tru9I TTAA 4 cut(s) 210, 474, 789, 1406
TscAI CASTG 5 cut(s) 505, 709, 1204, 1662, 1716
TseFI GTSAC 2 cut(s) 275, 911
TseI GCWGC 6 cut(s) 8, 152, 155, 322, 488, 1209
Tsp45I GTSAC 2 cut(s) 275, 911
TspDTI ATGAA 8 cut(s) 42, 459, 492, 632, 941, 1185, 1495, 1633
TspGWI ACGGA 2 cut(s) 726, 1588
TspRI CASTG 5 cut(s) 505, 709, 1204, 1662, 1716
Van91I CCANNNNNTGG 1 cut(s) 1661
VpaK11BI GGWCC 3 cut(s) 380, 734, 1627
VspI ATTAAT 1 cut(s) 789
XceI RCATGY 3 cut(s) 1078, 1294, 1490
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmnI GAANNNNTTC 2 cut(s) 790, 1118
XspI CTAG 6 cut(s) 413, 659, 893, 1340, 1491, 1755
Zsp2I ATGCAT 1 cut(s) 1488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.