Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0009472

Physical Location & Seq

Genomic Coordinates Reverse (-)

28082045 .. 28087653

Gene Structure 5609 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1232500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1767 bp

ATGGCGGCGGCTACTTCCTCCTTCTCTCCTGCCCTCTCCCCTGCTCCCCAATCATCCTCCGCATCCAAAACCCATGTGGCTCCCTCCAGTCTAGGGTTTTCATCCGCGGTCTCCAAGTCTCTCAGCCCTCTGAGAGCCAAGGCCACCCGCGGTGGTAATAATGTCTCCGGCGGCGGCTCAGCTCTCGGAGCTCGCATGGTGTCAATGCCGTCCATTAAGCCCCCTCCATCTCTTGATTTCGATACCTCTGTTTTCACCAAGGAGAAGATCAACCTCGCTGGTCACAACGAGTATATTGTGAGAGGAGGAAGGGATCTGTTCCACTTGCTGCCCGATGCTTTCAAGGGTATTAAGCAGATTGGTGTTATCGGTTGGGGTTCTCAGGGACCTGCTCAAGCTCAGAATCTAAGAGATTCTCTAGTTGAAGCAAAGTCTGACATTGTTGTCAAGATTGGACTCAGGAAGGGTTCTCGTTCTTTTGCTGAAGCTCGTGCAGCTGGTTTCACTGAGGAGAATGGGACTTTGGGGGATATATGGGAAACAATCTCAGGCAGTGATCTCGTGTTGCTACTAATCTCTGATTCTGCACAGGCCGATAATTATGAGAAAGTATTTTCACACATGAAACCAAATAGTATACTTGGTCTTTCCCATGGTTTTCTTCTAGGGCATTTGCAGTCAGTGGGACTTGACTTTCCCAAGAACTTCAGTGTAATTGCTGTATGTCCCAAGGGGATGGGTCCATCTGTAAGGAGACTTTATGTCCAAGGCAAAGAGATAAATGGTGCAGGAATTAATTCCAGTTTTGCAGTCCACCAGGATGTTGATGGTAGAGCCACAGATGTGGCCCTGGGCTGGTCAGTTGCCCTTGGTTCTCCTTTCACATTTGCCACTACACTAGAGCAGGAATACAAAAGTGACATCTTTGGCGAACGAGGCATATTACTTGGTGCTGTTCATGGAATTGTGGAGTCCCTGTTTAGAAGGTACACTGAAAATGGAATGAGTGAGGATTTGGCTTATAAAAACACTGTTGAATGTATAACTGGAATTGTGTCAAGGACCATCTCGGCTAAGGGAATGTTAGAGATTTACAACTCTTTGTCTGAAGATGGCAAAAAGGAATTTGAGGCTGCATATAGCGCCTCATTTTATCCATGCATGGACATCTTGTATGAATGCTATGAAGATGTTGCCAGTGGCAGCGAGATCCGCAGTGTTGTCTTGGCCGGTCGTCGCTTTTATGAGAAGGAGGGTCTGCCTGCATTCCCAATGGGTAAAATTGATCAGACCCGCATGTGGAAAGTCGGTGAGCGTGTCCGATCAACAAGGCCAGCTGGTGATCTAGGCCCATTACACCCTTTCACTGCGGGTGTTTTTGTTGCCTTAATGATGGCACAGATCGAAGTATTGAGGAAGAAAGGACATTCATATTCGGAGATCATTAATGAAAGTGTTATTGAATCGGTGGATTCTTTGAATCCTTTTATGCATGCACGGGGAGTTTCTTTCATGGTTGATAACTGTTCAACAACAGCTCGGTTGGGATCAAGGAAATGGGCTCCACGATTTGATTACATCCTTACTCAGCAGGCCTTAGTTTCTGTGGACAATGGTGCACCCATCAACCGAGACCTCATCAGCAACTTCATGTCAGATCCAGTGCATGGAGCCATCAAAGTATGCGCTGAACTGAGACCTACAGTTGACATTTCAGTGACTGCTGATGCAGATTTTGTGCGACCAGAGTTGCGTCAGAGCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

IlvC IlvN

Protein Analysis

589

Amino Acids

63.39

Weight (kDa)

6.66

Isoelectric Point (pI)

43.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KARI_N	PF07991	118 - 292	2.6e-30	Acetohydroxy acid isomeroreductase, NADPH-binding domain
KARI_C	PF01450	301 - 445	7.9e-34	Ketol-acid reductoisomerase, C-terminal domain
KARI_C	PF01450	457 - 527	4.5e-07	Ketol-acid reductoisomerase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009472)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58610 AT3G58610 AT3G58610
fragaria_vesca	FvH4_6g27150 FvH4_6g27150
malus_domestica	MD09G1239300.v1.1 MD17G1231900.v1.1 MD17G1232500.v1.1
prunus_persica	Prupe.3G122300_v2.0.a1 Prupe.3G122300_v2.0.a1
pyrus_communis	pycom09g15800 pycom17g23520
rosa_chinensis	RchiOBHm_Chr2g0129141
rosa_laevigata	RLG00000019058 RLG00000019060
rosa_multiflora	Rmu_sc0003479.1_g000002
rosa_roxburghii	Rroxscaffold_2G00114800
rosa_rugosa	Rorug02G0285100
rosa_samantha	Rh2BG345200 Rh2CG324600 Rh2DG362000
rosa_wichuraiana	Rw2G027350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1023
AasI	GACNNNNNNGTC	1 cut(s)	443
Acc36I	ACCTGC	1 cut(s)	397
AccB7I	CCANNNNNTGG	1 cut(s)	1667
AccI	GTMKAC	1 cut(s)	637
AccII	CGCG	2 cut(s)	107, 150
AclWI	GGATC	4 cut(s)	321, 1204, 1555, 1652
AcoI	YGGCCR	1 cut(s)	1227
AcsI	RAATTY	1 cut(s)	1124
AcuI	CTGAAG	3 cut(s)	504, 691, 1128
AfaI	GTAC	1 cut(s)	989
AfiI	CCNNNNNNNGG	4 cut(s)	93, 855, 1299, 1667
AgsI	TTSAA	6 cut(s)	343, 425, 1037, 1463, 1480, 1530
AhdI	GACNNNNNGTC	1 cut(s)	761
AjnI	CCWGG	2 cut(s)	816, 849
AjuI	GAANNNNNNNTTGG	2 cut(s)	506, 538
AleI	CACNNNNGTG	1 cut(s)	842
AluBI	AGCT	7 cut(s)	182, 191, 398, 488, 497, 1337, 1538
AluI	AGCT	7 cut(s)	182, 191, 398, 488, 497, 1337, 1538
Alw21I	GWGCWC	2 cut(s)	193, 1621
Alw26I	GTCTC	6 cut(s)	115, 123, 169, 748, 1626, 1690
Alw44I	GTGCAC	1 cut(s)	1617
AlwI	GGATC	4 cut(s)	321, 1204, 1555, 1652
AlwNI	CAGNNNCTG	1 cut(s)	1721
AoxI	GGCC	7 cut(s)	141, 591, 846, 1227, 1331, 1348, 1593
ApaLI	GTGCAC	1 cut(s)	1617
ApeKI	GCWGC	4 cut(s)	328, 494, 1133, 1203
ApoI	RAATTY	1 cut(s)	1124
AseI	ATTAAT	2 cut(s)	795, 1446
Asp700I	GAANNNNTTC	1 cut(s)	796
AspLEI	GCGC	2 cut(s)	1145, 1688
AspS9I	GGNCC	5 cut(s)	386, 740, 847, 1062, 1349
AsuHPI	GGTGA	3 cut(s)	247, 1322, 1352
AvaII	GGWCC	3 cut(s)	386, 740, 1062
BaeGI	GKGCMC	1 cut(s)	1621
BaeI	ACNNNNGTAYC	2 cut(s)	234, 267
BanII	GRGCYC	2 cut(s)	193, 1564
BauI	CACGAG	2 cut(s)	489, 560
Bbv12I	GWGCWC	2 cut(s)	193, 1621
BbvI	GCAGC	4 cut(s)	315, 506, 1120, 1215
BccI	CCATC	9 cut(s)	235, 730, 751, 821, 1073, 1106, 1387, 1631, 1682
BceAI	ACGGC	1 cut(s)	193
BcgI	CGANNNNNNTGC	2 cut(s)	541, 575
BciT130I	CCWGG	2 cut(s)	818, 851
BclI	TGATCA	1 cut(s)	1285
BcoDI	GTCTC	6 cut(s)	115, 123, 169, 748, 1626, 1690
BfaI	CTAG	5 cut(s)	92, 419, 665, 899, 1346
BfmI	CTRYAG	1 cut(s)	1701
BfoI	RGCGCY	1 cut(s)	1146
BfuAI	ACCTGC	1 cut(s)	397
BisI	GCNGC	8 cut(s)	6, 9, 172, 175, 329, 495, 1134, 1204
BlpI	GCTNAGC	1 cut(s)	178
BlsI	GCNGC	8 cut(s)	7, 10, 173, 176, 330, 496, 1135, 1205
Bme1390I	CCNGG	2 cut(s)	818, 851
Bme18I	GGWCC	3 cut(s)	386, 740, 1062
BmeRI	GACNNNNNGTC	1 cut(s)	761
BmgT120I	GGNCC	5 cut(s)	386, 740, 847, 1062, 1349
BmiI	GGNNCC	5 cut(s)	81, 387, 741, 1563, 1672
BmrFI	CCNGG	2 cut(s)	818, 851
BmsI	GCATC	3 cut(s)	71, 325, 1717
BpmI	CTGGAG	1 cut(s)	70
Bpu10I	CCTNAGC	1 cut(s)	1074
Bpu1102I	GCTNAGC	1 cut(s)	178
BpuEI	CTTGAG	1 cut(s)	378
BsaI	GGTCTC	3 cut(s)	115, 1626, 1690
BsaXI	ACNNNNNCTCC	2 cut(s)	962, 992
Bsc4I	CCNNNNNNNGG	4 cut(s)	93, 855, 1299, 1667
Bse118I	RCCGGY	1 cut(s)	1229
Bse1I	ACTGG	5 cut(s)	87, 801, 1051, 1197, 1661
BseBI	CCWGG	2 cut(s)	818, 851
BseGI	GGATG	6 cut(s)	53, 62, 101, 741, 826, 1578
BseLI	CCNNNNNNNGG	4 cut(s)	93, 855, 1299, 1667
BseNI	ACTGG	5 cut(s)	87, 801, 1051, 1197, 1661
BseRI	GAGGAG	2 cut(s)	318, 524
BseSI	GKGCMC	1 cut(s)	1621
BseXI	GCAGC	4 cut(s)	315, 506, 1120, 1215
BsgI	GTGCAG	3 cut(s)	513, 570, 807
Bsh1236I	CGCG	2 cut(s)	107, 150
Bsh1285I	CGRYCG	1 cut(s)	1234
BshFI	GGCC	7 cut(s)	143, 593, 848, 1229, 1333, 1350, 1595
BsiEI	CGRYCG	1 cut(s)	1234
BsiHKAI	GWGCWC	2 cut(s)	193, 1621
BsiSI	CCGG	2 cut(s)	168, 1230
BslFI	GGGAC	5 cut(s)	399, 532, 699, 711, 958
BslI	CCNNNNNNNGG	4 cut(s)	93, 855, 1299, 1667
BsmAI	GTCTC	6 cut(s)	115, 123, 169, 748, 1626, 1690
BsmFI	GGGAC	5 cut(s)	399, 532, 699, 711, 958
BsmI	GAATGC	2 cut(s)	1184, 1265
BsnI	GGCC	7 cut(s)	143, 593, 848, 1229, 1333, 1350, 1595
Bso31I	GGTCTC	3 cut(s)	115, 1626, 1690
Bsp1286I	GDGCHC	3 cut(s)	193, 1564, 1621
Bsp1720I	GCTNAGC	1 cut(s)	178
Bsp19I	CCATGG	1 cut(s)	652
BspANI	GGCC	7 cut(s)	143, 593, 848, 1229, 1333, 1350, 1595
BspFNI	CGCG	2 cut(s)	107, 150
BspLI	GGNNCC	5 cut(s)	81, 387, 741, 1563, 1672
BspMI	ACCTGC	1 cut(s)	397
BspPI	GGATC	4 cut(s)	321, 1204, 1555, 1652
BspTNI	GGTCTC	3 cut(s)	115, 1626, 1690
BsrFI	RCCGGY	1 cut(s)	1229
BsrI	ACTGG	5 cut(s)	87, 801, 1051, 1197, 1661
BssAI	RCCGGY	1 cut(s)	1229
BssNAI	GTATAC	1 cut(s)	638
BssSI	CACGAG	2 cut(s)	489, 560
BssT1I	CCWWGG	6 cut(s)	138, 258, 652, 729, 766, 868
Bst1107I	GTATAC	1 cut(s)	638
Bst2BI	CACGAG	2 cut(s)	489, 560
Bst2UI	CCWGG	2 cut(s)	818, 851
Bst4CI	ACNGT	3 cut(s)	1033, 1526, 1705
BstC8I	GCNNGC	5 cut(s)	193, 1263, 1335, 1494, 1593
BstDSI	CCRYGG	3 cut(s)	105, 148, 652
BstF5I	GGATG	6 cut(s)	53, 62, 101, 741, 826, 1578
BstFNI	CGCG	2 cut(s)	107, 150
BstH2I	RGCGCY	1 cut(s)	1146
BstHHI	GCGC	2 cut(s)	1145, 1688
BstMAI	GTCTC	6 cut(s)	115, 123, 169, 748, 1626, 1690
BstMCI	CGRYCG	1 cut(s)	1234
BstMWI	GCNNNNNNNGC	5 cut(s)	188, 494, 936, 1142, 1212
BstNI	CCWGG	2 cut(s)	818, 851
BstNSI	RCATGY	2 cut(s)	1300, 1496
BstSCI	CCNGG	2 cut(s)	816, 849
BstSFI	CTRYAG	1 cut(s)	1701
BstSLI	GKGCMC	1 cut(s)	1621
BstUI	CGCG	2 cut(s)	107, 150
BstV1I	GCAGC	4 cut(s)	315, 506, 1120, 1215
BstX2I	RGATCY	3 cut(s)	313, 1209, 1657
BstXI	CCANNNNNNTGG	2 cut(s)	736, 844
BstYI	RGATCY	3 cut(s)	313, 1209, 1657
BstZ17I	GTATAC	1 cut(s)	638
BsuRI	GGCC	7 cut(s)	143, 593, 848, 1229, 1333, 1350, 1595
BtgI	CCRYGG	3 cut(s)	105, 148, 652
BtsCI	GGATG	6 cut(s)	53, 62, 101, 741, 826, 1578
BtsI	GCAGTG	3 cut(s)	559, 1222, 1365
BveI	ACCTGC	1 cut(s)	397
Cac8I	GCNNGC	5 cut(s)	193, 1263, 1335, 1494, 1593
CaiI	CAGNNNCTG	1 cut(s)	1721
CfoI	GCGC	2 cut(s)	1145, 1688
Cfr10I	RCCGGY	1 cut(s)	1229
Cfr13I	GGNCC	5 cut(s)	386, 740, 847, 1062, 1349
Cfr42I	CCGCGG	2 cut(s)	108, 151
CseI	GACGC	1 cut(s)	1742
Csp6I	GTAC	1 cut(s)	988
CviQI	GTAC	1 cut(s)	988
DrdI	GACNNNNNNGTC	1 cut(s)	443
DriI	GACNNNNNGTC	1 cut(s)	761
DseDI	GACNNNNNNGTC	1 cut(s)	443
EaeI	YGGCCR	1 cut(s)	1227
Eam1105I	GACNNNNNGTC	1 cut(s)	761
Ecl136II	GAGCTC	1 cut(s)	191
Eco130I	CCWWGG	6 cut(s)	138, 258, 652, 729, 766, 868
Eco147I	AGGCCT	1 cut(s)	1595
Eco24I	GRGCYC	2 cut(s)	193, 1564
Eco31I	GGTCTC	3 cut(s)	115, 1626, 1690
Eco47I	GGWCC	3 cut(s)	386, 740, 1062
Eco53kI	GAGCTC	1 cut(s)	191
Eco57I	CTGAAG	3 cut(s)	504, 691, 1128
EcoICRI	GAGCTC	1 cut(s)	191
EcoO109I	RGGNCCY	1 cut(s)	386
EcoRII	CCWGG	2 cut(s)	816, 849
EcoT14I	CCWWGG	6 cut(s)	138, 258, 652, 729, 766, 868
EcoT22I	ATGCAT	2 cut(s)	1163, 1494
EcoT38I	GRGCYC	2 cut(s)	193, 1564
ErhI	CCWWGG	6 cut(s)	138, 258, 652, 729, 766, 868
FaqI	GGGAC	5 cut(s)	399, 532, 699, 711, 958
FauI	CCCGC	3 cut(s)	155, 1301, 1363
FbaI	TGATCA	1 cut(s)	1285
FblI	GTMKAC	1 cut(s)	637
Fnu4HI	GCNGC	8 cut(s)	6, 9, 172, 175, 329, 495, 1134, 1204
FokI	GGATG	6 cut(s)	40, 49, 88, 748, 833, 1565
FriOI	GRGCYC	2 cut(s)	193, 1564
Fsp4HI	GCNGC	8 cut(s)	6, 9, 172, 175, 329, 495, 1134, 1204
FspBI	CTAG	5 cut(s)	92, 419, 665, 899, 1346
GlaI	GCGC	2 cut(s)	1144, 1687
GluI	GCNGC	8 cut(s)	6, 9, 172, 175, 329, 495, 1134, 1204
GsuI	CTGGAG	1 cut(s)	70
HaeII	RGCGCY	1 cut(s)	1146
HaeIII	GGCC	7 cut(s)	143, 593, 848, 1229, 1333, 1350, 1595
HapII	CCGG	2 cut(s)	168, 1230
HgaI	GACGC	1 cut(s)	1742
HhaI	GCGC	2 cut(s)	1145, 1688
Hin6I	GCGC	2 cut(s)	1143, 1686
HinP1I	GCGC	2 cut(s)	1143, 1686
HincII	GTYRAC	1 cut(s)	1708
HindII	GTYRAC	1 cut(s)	1708
HinfI	GANTC	8 cut(s)	403, 413, 456, 581, 971, 1463, 1472, 1480
HpaII	CCGG	2 cut(s)	168, 1230
HphI	GGTGA	3 cut(s)	247, 1322, 1352
Hpy166II	GTNNAC	6 cut(s)	638, 814, 990, 1609, 1619, 1708
Hpy188III	TCNNGA	3 cut(s)	233, 448, 460
Hpy8I	GTNNAC	6 cut(s)	638, 814, 990, 1609, 1619, 1708
Hpy99I	CGWCG	1 cut(s)	1239
HpyAV	CCTTC	5 cut(s)	31, 303, 457, 978, 1243
HpyCH4III	ACNGT	3 cut(s)	1033, 1526, 1705
HpyF10VI	GCNNNNNNNGC	5 cut(s)	188, 494, 936, 1142, 1212
HspAI	GCGC	2 cut(s)	1143, 1686
Ksp22I	TGATCA	1 cut(s)	1285
KspI	CCGCGG	2 cut(s)	108, 151
LmnI	GCTCC	5 cut(s)	49, 85, 188, 1567, 1670
Lsp1109I	GCAGC	4 cut(s)	315, 506, 1120, 1215
LweI	GCATC	3 cut(s)	71, 325, 1717
MaeI	CTAG	5 cut(s)	92, 419, 665, 899, 1346
MaeIII	GTNAC	3 cut(s)	281, 917, 1717
MboII	GAAGA	5 cut(s)	277, 653, 1121, 1199, 1429
MflI	RGATCY	3 cut(s)	313, 1209, 1657
MhlI	GDGCHC	3 cut(s)	193, 1564, 1621
MluCI	AATT	8 cut(s)	598, 714, 792, 796, 963, 1050, 1124, 1281
MlyI	GAGTC	2 cut(s)	450, 980
Mph1103I	ATGCAT	2 cut(s)	1163, 1494
MroXI	GAANNNNTTC	1 cut(s)	796
MseI	TTAA	5 cut(s)	216, 351, 795, 1388, 1446
MslI	CAYNNNNRTG	3 cut(s)	819, 842, 1715
MspA1I	CMGCKG	4 cut(s)	107, 150, 497, 1337
MspI	CCGG	2 cut(s)	168, 1230
MspR9I	CCNGG	2 cut(s)	818, 851
Mva1269I	GAATGC	2 cut(s)	1184, 1265
MvaI	CCWGG	2 cut(s)	818, 851
MvnI	CGCG	2 cut(s)	107, 150
MwoI	GCNNNNNNNGC	5 cut(s)	188, 494, 936, 1142, 1212
NcoI	CCATGG	1 cut(s)	652
NlaIV	GGNNCC	5 cut(s)	81, 387, 741, 1563, 1672
NmeAIII	GCCGAG	1 cut(s)	1049
NmuCI	GTSAC	3 cut(s)	281, 917, 1717
NsiI	ATGCAT	2 cut(s)	1163, 1494
NspI	RCATGY	2 cut(s)	1300, 1496
OliI	CACNNNNGTG	1 cut(s)	842
PaeI	GCATGC	1 cut(s)	1496
PasI	CCCWGGG	1 cut(s)	850
PceI	AGGCCT	1 cut(s)	1595
PctI	GAATGC	2 cut(s)	1184, 1265
PdmI	GAANNNNTTC	1 cut(s)	796
PfeI	GAWTC	6 cut(s)	403, 413, 581, 1463, 1472, 1480
PflMI	CCANNNNNTGG	1 cut(s)	1667
PkrI	GCNGC	8 cut(s)	7, 10, 173, 176, 330, 496, 1135, 1205
PleI	GAGTC	2 cut(s)	450, 979
PpsI	GAGTC	2 cut(s)	450, 979
PpuMI	RGGWCCY	1 cut(s)	386
PshBI	ATTAAT	2 cut(s)	795, 1446
PsiI	TTATAA	1 cut(s)	1023
Psp124BI	GAGCTC	1 cut(s)	193
Psp5II	RGGWCCY	1 cut(s)	386
Psp6I	CCWGG	2 cut(s)	816, 849
PspGI	CCWGG	2 cut(s)	816, 849
PspN4I	GGNNCC	5 cut(s)	81, 387, 741, 1563, 1672
PspPI	GGNCC	5 cut(s)	386, 740, 847, 1062, 1349
PspPPI	RGGWCCY	1 cut(s)	386
PstNI	CAGNNNCTG	1 cut(s)	1721
PsuI	RGATCY	3 cut(s)	313, 1209, 1657
PvuII	CAGCTG	2 cut(s)	497, 1337
RsaI	GTAC	1 cut(s)	989
RsaNI	GTAC	1 cut(s)	988
RseI	CAYNNNNRTG	3 cut(s)	819, 842, 1715
SacI	GAGCTC	1 cut(s)	193
SacII	CCGCGG	2 cut(s)	108, 151
SaqAI	TTAA	5 cut(s)	216, 351, 795, 1388, 1446
SatI	GCNGC	8 cut(s)	6, 9, 172, 175, 329, 495, 1134, 1204
Sau96I	GGNCC	5 cut(s)	386, 740, 847, 1062, 1349
SchI	GAGTC	2 cut(s)	450, 980
ScrFI	CCNGG	2 cut(s)	818, 851
SduI	GDGCHC	3 cut(s)	193, 1564, 1621
SfaNI	GCATC	3 cut(s)	71, 325, 1717
SfcI	CTRYAG	1 cut(s)	1701
Sfr303I	CCGCGG	2 cut(s)	108, 151
SgrBI	CCGCGG	2 cut(s)	108, 151
SinI	GGWCC	3 cut(s)	386, 740, 1062
SmiMI	CAYNNNNRTG	3 cut(s)	819, 842, 1715
SmlI	CTYRAG	1 cut(s)	393
SmoI	CTYRAG	1 cut(s)	393
SphI	GCATGC	1 cut(s)	1496
Sse9I	AATT	8 cut(s)	598, 714, 792, 796, 963, 1050, 1124, 1281
SseBI	AGGCCT	1 cut(s)	1595
SspMI	CTAG	5 cut(s)	92, 419, 665, 899, 1346
SstI	GAGCTC	1 cut(s)	193
StuI	AGGCCT	1 cut(s)	1595
StyD4I	CCNGG	2 cut(s)	816, 849
StyI	CCWWGG	6 cut(s)	138, 258, 652, 729, 766, 868
TaaI	ACNGT	3 cut(s)	1033, 1526, 1705
TaqI	TCGA	2 cut(s)	240, 1404
TasI	AATT	8 cut(s)	598, 714, 792, 796, 963, 1050, 1124, 1281
TauI	GCSGC	4 cut(s)	8, 11, 174, 177
TfiI	GAWTC	6 cut(s)	403, 413, 581, 1463, 1472, 1480
Tru1I	TTAA	5 cut(s)	216, 351, 795, 1388, 1446
Tru9I	TTAA	5 cut(s)	216, 351, 795, 1388, 1446
TseFI	GTSAC	3 cut(s)	281, 917, 1717
TseI	GCWGC	4 cut(s)	328, 494, 1133, 1203
Tsp45I	GTSAC	3 cut(s)	281, 917, 1717
TspDTI	ATGAA	9 cut(s)	90, 638, 947, 1191, 1200, 1419, 1464, 1501, 1639
Van91I	CCANNNNNTGG	1 cut(s)	1667
VneI	GTGCAC	1 cut(s)	1617
VpaK11BI	GGWCC	3 cut(s)	386, 740, 1062
VspI	ATTAAT	2 cut(s)	795, 1446
XapI	RAATTY	1 cut(s)	1124
XceI	RCATGY	2 cut(s)	1300, 1496
XcmI	CCANNNNNNNNNTGG	2 cut(s)	73, 824
XmiI	GTMKAC	1 cut(s)	637
XmnI	GAANNNNTTC	1 cut(s)	796
XspI	CTAG	5 cut(s)	92, 419, 665, 899, 1346
Zsp2I	ATGCAT	2 cut(s)	1163, 1494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.