FvH4_6g30000

DCC family protein At1g52590

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014125
Physical Location & Seq
Reverse (-)
23183085 .. 23187521
4437 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30000.t1

Sequence Viewer

Length: 531 bp
ATGGCGCTTCTGCTTCCTGGCGGGTGCATCCGAGTAAGGCCACCACTCAGCGAGTCTCGGCCAGCTCACGTTAGACGGAAGCTCACTACTTTTGCTACTGTATCCCCAACTCGAGGAGAATCAGTTGACTGGGTCCAAGCAACTTCATCCTTCTTTGAAGACGACACAAGACCCATCATGCTATTTGACGGTGTTTGCAACTTGTGTAATGGAGGTGTGAAGTTCGTGCGTGACAACGATCGAAACAGGAGAATGAGGTTTGAAGCTCTCCAGAGTGAAGCAGGGAGGAAACTGCTACAAAGATCGGGGAGAGCTCCTGATGATATATCTAGTGTTGTGCTGGTTGAAAAGGACAGATCATTCATAAAGTCAGAAGCTGTTGTGAAGATAATGGAACACATAGACCTACCCTTCCCCCAACTAGCATTCTTCTTACAGTTTGTTCCCCTCTTCATACGGGATTTCATATATGACAATGTTGCAAACAATCGTTACAGCATTTTTGGTCGCTCAGAGTCGTGTGAGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF393

Protein Analysis

177

Amino Acids

20.07

Weight (kDa)

8.8

Isoelectric Point (pI)

60.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCC1-like PF04134 61 - 170 5.2e-27 DCC1-like thiol-disulfide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52590
fragaria_vesca FvH4_6g30000
malus_domestica MD00G1022200.v1.1
prunus_persica Prupe.3G083500_v2.0.a1 Prupe.3G083500_v2.0.a1 Prupe.3G083500_v2.0.a1
pyrus_communis pycom17g21400
rosa_chinensis RchiOBHm_Chr2g0136021
rosa_laevigata RLG00000019497
rosa_multiflora Rmu_ssc0000343.1_g000029
rosa_roxburghii Rroxscaffold_2G00108690
rosa_rugosa Rorug02G0327700.1
rosa_samantha Rh2AG379400 Rh2BG385800 Rh2CG365000 Rh2DG402200
rosa_wichuraiana Rw2G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 113
AgsI TTSAA 3 cut(s) 158, 263, 347
AjnI CCWGG 1 cut(s) 16
AluBI AGCT 5 cut(s) 65, 82, 266, 314, 377
AluI AGCT 5 cut(s) 65, 82, 266, 314, 377
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 1 cut(s) 60
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 111
AoxI GGCC 2 cut(s) 38, 59
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 133
AvaI CYCGRG 1 cut(s) 111
AvaII GGWCC 1 cut(s) 133
BanII GRGCYC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 316
BccI CCATC 1 cut(s) 182
BciT130I CCWGG 1 cut(s) 18
BciVI GTATCC 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 60
BfaI CTAG 2 cut(s) 330, 422
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 18
Bme18I GGWCC 1 cut(s) 133
BmeT110I CYCGRG 1 cut(s) 111
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 18
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 1 cut(s) 36
BmuI ACTGGG 1 cut(s) 139
BpiI GAAGAC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 254
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 1 cut(s) 113
Bse1I ACTGG 1 cut(s) 134
BseBI CCWGG 1 cut(s) 18
BseGI GGATG 2 cut(s) 27, 146
BseLI CCNNNNNNNGG 1 cut(s) 113
BseMII CTCAG 2 cut(s) 61, 525
BseNI ACTGG 1 cut(s) 134
BseRI GAGGAG 1 cut(s) 129
Bsh1285I CGRYCG 1 cut(s) 241
BshFI GGCC 2 cut(s) 40, 61
BsiEI CGRYCG 1 cut(s) 241
BsiHKAI GWGCWC 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 111
BslI CCNNNNNNNGG 1 cut(s) 113
BsmAI GTCTC 1 cut(s) 60
BsmI GAATGC 1 cut(s) 425
BsnI GGCC 2 cut(s) 40, 61
BsoBI CYCGRG 1 cut(s) 111
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 3 cut(s) 238, 302, 356
BspACI CCGC 1 cut(s) 21
BspANI GGCC 2 cut(s) 40, 61
BspCNI CTCAG 2 cut(s) 60, 524
BspLI GGNNCC 1 cut(s) 134
BsrI ACTGG 1 cut(s) 134
BssMI GATC 3 cut(s) 238, 302, 356
Bst2UI CCWGG 1 cut(s) 18
Bst4CI ACNGT 3 cut(s) 100, 191, 438
Bst6I CTCTTC 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 2 cut(s) 47, 511
BstF5I GGATG 2 cut(s) 27, 146
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 241, 305, 359
BstMAI GTCTC 1 cut(s) 60
BstMBI GATC 3 cut(s) 238, 302, 356
BstMCI CGRYCG 1 cut(s) 241
BstNI CCWGG 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 165
BsuI GTATCC 1 cut(s) 112
BsuRI GGCC 2 cut(s) 40, 61
BtsCI GGATG 2 cut(s) 27, 146
Cac8I GCNNGC 1 cut(s) 63
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 133
CviAII CATG 1 cut(s) 178
CviJI RGCY 7 cut(s) 40, 61, 65, 82, 266, 314, 377
CviKI_1 RGCY 7 cut(s) 40, 61, 65, 82, 266, 314, 377
DdeI CTNAG 2 cut(s) 47, 511
DpnI GATC 3 cut(s) 240, 304, 358
DpnII GATC 3 cut(s) 238, 302, 356
EaeI YGGCCR 1 cut(s) 59
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
Ecl136II GAGCTC 1 cut(s) 314
Eco24I GRGCYC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 314
Eco88I CYCGRG 1 cut(s) 111
EcoICRI GAGCTC 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 316
FaeI CATG 1 cut(s) 181
FaiI YATR 9 cut(s) 179, 326, 365, 401, 455, 467, 469, 471, 529
FatI CATG 1 cut(s) 177
FauI CCCGC 1 cut(s) 14
FokI GGATG 2 cut(s) 14, 133
FriOI GRGCYC 1 cut(s) 316
FspBI CTAG 2 cut(s) 330, 422
GlaI GCGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 254
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 40, 61
HhaI GCGC 1 cut(s) 7
Hin1II CATG 1 cut(s) 181
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 3 cut(s) 53, 119, 515
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 3 cut(s) 32, 373, 514
Hpy188III TCNNGA 2 cut(s) 271, 317
Hpy8I GTNNAC 1 cut(s) 127
HpyAV CCTTC 2 cut(s) 160, 421
HpyCH4III ACNGT 3 cut(s) 100, 191, 438
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 3 cut(s) 27, 198, 482
HpyF3I CTNAG 2 cut(s) 47, 511
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 1 cut(s) 181
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 3 cut(s) 238, 302, 356
LmnI GCTCC 1 cut(s) 319
LpnPI CCDG 9 cut(s) 3, 30, 75, 115, 232, 267, 284, 326, 330
LweI GCATC 1 cut(s) 36
MaeI CTAG 2 cut(s) 330, 422
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 2 cut(s) 230, 491
MalI GATC 3 cut(s) 240, 304, 358
MboI GATC 3 cut(s) 238, 302, 356
MboII GAAGA 4 cut(s) 170, 397, 421, 442
MhlI GDGCHC 1 cut(s) 316
MlyI GAGTC 2 cut(s) 62, 524
MnlI CCTC 5 cut(s) 107, 206, 249, 279, 458
MspR9I CCNGG 1 cut(s) 18
Mva1269I GAATGC 1 cut(s) 425
MvaI CCWGG 1 cut(s) 18
NdeII GATC 3 cut(s) 238, 302, 356
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 1 cut(s) 134
NmeAIII GCCGAG 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 230
PaeR7I CTCGAG 1 cut(s) 111
PctI GAATGC 1 cut(s) 425
PfeI GAWTC 1 cut(s) 119
PflFI GACNNNGTC 1 cut(s) 131
Ple19I CGATCG 1 cut(s) 241
PleI GAGTC 2 cut(s) 61, 523
PpsI GAGTC 2 cut(s) 61, 523
Psp124BI GAGCTC 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 134
PspPI GGNCC 1 cut(s) 133
PspXI VCTCGAGB 1 cut(s) 111
PstNI CAGNNNCTG 1 cut(s) 377
PsyI GACNNNGTC 1 cut(s) 131
PvuI CGATCG 1 cut(s) 241
SacI GAGCTC 1 cut(s) 316
Sau3AI GATC 3 cut(s) 238, 302, 356
Sau96I GGNCC 1 cut(s) 133
SchI GAGTC 2 cut(s) 62, 524
ScrFI CCNGG 1 cut(s) 18
SduI GDGCHC 1 cut(s) 316
SetI ASST 9 cut(s) 67, 72, 84, 217, 260, 268, 316, 379, 408
SfaNI GCATC 1 cut(s) 36
Sfr274I CTCGAG 1 cut(s) 111
SinI GGWCC 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
SsiI CCGC 1 cut(s) 21
SspMI CTAG 2 cut(s) 330, 422
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 1 cut(s) 16
TaaI ACNGT 3 cut(s) 100, 191, 438
TaiI ACGT 1 cut(s) 72
TaqI TCGA 2 cut(s) 112, 241
TfiI GAWTC 1 cut(s) 119
TseFI GTSAC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 4 cut(s) 135, 352, 442, 454
TspGWI ACGGA 1 cut(s) 91
Tth111I GACNNNGTC 1 cut(s) 131
VpaK11BI GGWCC 1 cut(s) 133
XhoI CTCGAG 1 cut(s) 111
XspI CTAG 2 cut(s) 330, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.