FvH4_6g32300

non-haem dioxygenase in morphine synthesis N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014284
Physical Location & Seq
Reverse (-)
25340182 .. 25343007
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32300.t1

Sequence Viewer

Length: 1119 bp
ATGGCAAACTTACTTGAACCCGTCCACGAAAACCCTATTGATTTCCGTGCCCCGCCACCTTCTCCAGTTGCGTCTGGCCGCCGATCATCCCTCACAAACGACGATGTCATTACCGAATTCCTCCAGCACTCCCTCAATGTCCCTGACCTCATCTTGCCAGACAAGTTCTTCCCAAAACAAAAATCAGTTGAAAATCCCCCCACAATTGATTTTGAAACATTGTGTTCACATCATTATGATGATGCTAGTGTTGCCAAGATTCTGGAGTCCATAGCAAGAATCGGGTGCTTTCAGTTAGTGAACCATGGGATTTCAAGTGATTTTGTAAGGTCAGTGCAGGCTGAAGCCATCGGGATATTCCAGGTGCCACTGGAGAAGCGGGCAGACATAACAAGGTCACCGGAGAAGACATATGGGTTCGAGGAAGTTCACGGTGAGGAGGCTGACACGGAATTGAGTGAAGAGTTTGTTTGGTGTAGAGAACAAGGTTTGAAGTTGGCAATGGAGGGAATTTGGCCAATAGGGTATTCAAATTTCAGCAAGAAATTGGAGATCCTTATGACAGATACAGAGAAGGTTTGTGAGAGAATTCTACAAAGCTTATTGAACACTAAACAAAAGGAATCTGTTTATGGAAATACTTTTGTTCAGGGAAAAGAACTTGGGACAGTTTGTTGTTTTTACAAGCACAATCAGAATGTTTTAGCGGATAAGTGGGATAGCTATCTGAGATATGATGTGATTAGGATGCTGATAAGAGGAACTGATTACTCTCATGCCTTGTGTTTGCATCTTTGTGATGGATCCTCAGAGTTTCATGTTTACTCCAAGAAAGGTTGGGTTTCTTTTACCCCAGATAGAGATGCTCTTGTAGTTACCACAGGGGACCAAATGCAGGCATGGAGTGGAAGCCAATACAAGCATGTGATAGGAAGGCCAATCTTTCAGGCCAAGGAGGAATGCATTTCAATGGCCCTTCTCTATTCCCCTCTGAACATCATGACCAACTCCCAAGGCAACACGGAAAAGAAAATTTTACTCGGCCAACAATTTATAGCGGCTATATTACTAACTCTTGTCTACCAATTTTCAGTATACTACTTATACATGAATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

373

Amino Acids

42.26

Weight (kDa)

5.42

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 67 - 151 2.2e-13 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014284)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79760 AT1G79760
fragaria_vesca FvH4_6g32300 FvH4_6g32300
malus_domestica MD09G1204500.v1.1
prunus_persica Prupe.3G060100_v2.0.a1
pyrus_communis pycom09g12140
rosa_chinensis RchiOBHm_Chr2g0140341
rosa_laevigata RLG00000019860
rosa_multiflora Rmu_sc0005446.1_g000032
rosa_roxburghii Rroxscaffold_2G00104920
rosa_rugosa Rorug02G0361700
rosa_samantha Rh2AG413000 Rh2BG423400 Rh2CG399000 Rh2DG432300
rosa_wichuraiana Rw2G033850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 364
AccI GTMKAC 2 cut(s) 1080, 1095
AciI CCGC 5 cut(s) 53, 79, 379, 707, 1058
AclWI GGATC 3 cut(s) 547, 798, 811
AcoI YGGCCR 3 cut(s) 76, 515, 1042
AcsI RAATTY 5 cut(s) 116, 510, 532, 588, 1032
AcuI CTGAAG 1 cut(s) 363
AfiI CCNNNNNNNGG 1 cut(s) 895
AgsI TTSAA 8 cut(s) 17, 191, 215, 315, 493, 531, 607, 969
AjnI CCWGG 1 cut(s) 360
AjuI GAANNNNNNNTTGG 2 cut(s) 511, 543
AluBI AGCT 2 cut(s) 600, 723
AluI AGCT 2 cut(s) 600, 723
AlwI GGATC 3 cut(s) 547, 798, 811
AoxI GGCC 6 cut(s) 76, 515, 935, 948, 972, 1042
ApoI RAATTY 5 cut(s) 116, 510, 532, 588, 1032
AspS9I GGNCC 2 cut(s) 886, 973
AsuHPI GGTGA 2 cut(s) 390, 446
AvaII GGWCC 1 cut(s) 886
BaeGI GKGCMC 1 cut(s) 52
BalI TGGCCA 1 cut(s) 517
BamHI GGATCC 1 cut(s) 803
BanI GGYRCC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 413
BccI CCATC 2 cut(s) 356, 794
BciT130I CCWGG 1 cut(s) 362
BfaI CTAG 1 cut(s) 246
BisI GCNGC 2 cut(s) 79, 1059
BlsI GCNGC 2 cut(s) 80, 1060
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 886
BmgT120I GGNCC 2 cut(s) 886, 973
BmiI GGNNCC 3 cut(s) 366, 805, 887
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 4 cut(s) 232, 738, 799, 853
BpiI GAAGAC 1 cut(s) 413
BpmI CTGGAG 4 cut(s) 48, 107, 284, 392
BsaBI GATNNNNATC 1 cut(s) 723
BsaJI CCNNGG 3 cut(s) 304, 951, 1012
BsaWI WCCGGW 1 cut(s) 400
Bsc4I CCNNNNNNNGG 1 cut(s) 895
Bse1I ACTGG 2 cut(s) 65, 375
Bse3DI GCAATG 1 cut(s) 507
Bse8I GATNNNNATC 1 cut(s) 723
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 3 cut(s) 304, 951, 1012
BseGI GGATG 2 cut(s) 86, 753
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 1 cut(s) 895
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 2 cut(s) 719, 822
BseNI ACTGG 2 cut(s) 65, 375
BseRI GAGGAG 1 cut(s) 452
BseSI GKGCMC 1 cut(s) 52
BsgI GTGCAG 1 cut(s) 356
BshFI GGCC 6 cut(s) 78, 517, 937, 950, 974, 1044
BshNI GGYRCC 1 cut(s) 364
BsiSI CCGG 1 cut(s) 401
BslFI GGGAC 3 cut(s) 125, 679, 899
BslI CCNNNNNNNGG 1 cut(s) 895
BsmFI GGGAC 3 cut(s) 125, 679, 899
BsmI GAATGC 1 cut(s) 965
BsnI GGCC 6 cut(s) 78, 517, 937, 950, 974, 1044
Bsp1286I GDGCHC 1 cut(s) 52
Bsp143I GATC 3 cut(s) 83, 552, 803
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 5 cut(s) 53, 79, 379, 707, 1058
BspANI GGCC 6 cut(s) 78, 517, 937, 950, 974, 1044
BspCNI CTCAG 2 cut(s) 720, 821
BspHI TCATGA 1 cut(s) 999
BspLI GGNNCC 3 cut(s) 366, 805, 887
BspPI GGATC 3 cut(s) 547, 798, 811
BspT107I GGYRCC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 2 cut(s) 65, 375
BssECI CCNNGG 3 cut(s) 304, 951, 1012
BssMI GATC 3 cut(s) 83, 552, 803
BssNAI GTATAC 1 cut(s) 1096
BssT1I CCWWGG 3 cut(s) 304, 951, 1012
Bst1107I GTATAC 1 cut(s) 1096
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 434, 670
Bst6I CTCTTC 1 cut(s) 456
BstC8I GCNNGC 3 cut(s) 339, 381, 897
BstDEI CTNAG 2 cut(s) 728, 808
BstDSI CCRYGG 1 cut(s) 304
BstEII GGTNACC 1 cut(s) 396
BstF5I GGATG 2 cut(s) 86, 753
BstKTI GATC 3 cut(s) 86, 555, 806
BstMBI GATC 3 cut(s) 83, 552, 803
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 1 cut(s) 362
BstNSI RCATGY 1 cut(s) 926
BstPI GGTNACC 1 cut(s) 396
BstSCI CCNGG 1 cut(s) 360
BstSLI GKGCMC 1 cut(s) 52
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 2 cut(s) 552, 803
BstXI CCANNNNNNTGG 1 cut(s) 262
BstYI RGATCY 2 cut(s) 552, 803
BstZ17I GTATAC 1 cut(s) 1096
BsuRI GGCC 6 cut(s) 78, 517, 937, 950, 974, 1044
BtgI CCRYGG 1 cut(s) 304
BtsCI GGATG 2 cut(s) 86, 753
BtsIMutI CAGTG 2 cut(s) 339, 368
Cac8I GCNNGC 3 cut(s) 339, 381, 897
CciI TCATGA 1 cut(s) 999
Cfr13I GGNCC 2 cut(s) 886, 973
CseI GACGC 1 cut(s) 60
CviAII CATG 7 cut(s) 305, 776, 818, 900, 923, 1000, 1108
DdeI CTNAG 2 cut(s) 728, 808
DpnI GATC 3 cut(s) 85, 554, 805
DpnII GATC 3 cut(s) 83, 552, 803
EaeI YGGCCR 3 cut(s) 76, 515, 1042
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco130I CCWWGG 3 cut(s) 304, 951, 1012
Eco47I GGWCC 1 cut(s) 886
Eco57I CTGAAG 1 cut(s) 363
Eco91I GGTNACC 1 cut(s) 396
EcoO65I GGTNACC 1 cut(s) 396
EcoRI GAATTC 2 cut(s) 116, 588
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 3 cut(s) 304, 951, 1012
EcoT22I ATGCAT 1 cut(s) 965
ErhI CCWWGG 3 cut(s) 304, 951, 1012
FaeI CATG 7 cut(s) 308, 779, 821, 903, 926, 1003, 1111
FaqI GGGAC 3 cut(s) 125, 679, 899
FatI CATG 7 cut(s) 304, 775, 817, 899, 922, 999, 1107
FauI CCCGC 2 cut(s) 60, 372
FauNDI CATATG 1 cut(s) 412
FblI GTMKAC 2 cut(s) 1080, 1095
Fnu4HI GCNGC 2 cut(s) 79, 1059
FokI GGATG 2 cut(s) 73, 760
Fsp4HI GCNGC 2 cut(s) 79, 1059
FspBI CTAG 1 cut(s) 246
GluI GCNGC 2 cut(s) 79, 1059
GsuI CTGGAG 4 cut(s) 48, 107, 284, 392
HaeIII GGCC 6 cut(s) 78, 517, 937, 950, 974, 1044
HapII CCGG 1 cut(s) 401
HgaI GACGC 1 cut(s) 60
Hin1II CATG 7 cut(s) 308, 779, 821, 903, 926, 1003, 1111
HindIII AAGCTT 1 cut(s) 598
HinfI GANTC 4 cut(s) 259, 266, 279, 623
HpaII CCGG 1 cut(s) 401
HphI GGTGA 2 cut(s) 390, 446
Hpy166II GTNNAC 7 cut(s) 25, 227, 301, 430, 823, 1081, 1096
Hpy188I TCNGA 4 cut(s) 696, 729, 811, 993
Hpy188III TCNNGA 3 cut(s) 263, 352, 1000
Hpy8I GTNNAC 7 cut(s) 25, 227, 301, 430, 823, 1081, 1096
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 4 cut(s) 69, 568, 927, 986
HpyCH4III ACNGT 2 cut(s) 434, 670
HpyCH4V TGCA 4 cut(s) 337, 790, 895, 963
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 2 cut(s) 728, 808
Hsp92II CATG 7 cut(s) 308, 779, 821, 903, 926, 1003, 1111
Kzo9I GATC 3 cut(s) 83, 552, 803
LweI GCATC 4 cut(s) 232, 738, 799, 853
MaeI CTAG 1 cut(s) 246
MaeIII GTNAC 2 cut(s) 396, 874
MalI GATC 3 cut(s) 85, 554, 805
MboI GATC 3 cut(s) 83, 552, 803
MboII GAAGA 3 cut(s) 160, 418, 473
MfeI CAATTG 1 cut(s) 204
MflI RGATCY 2 cut(s) 552, 803
MhlI GDGCHC 1 cut(s) 52
MlsI TGGCCA 1 cut(s) 517
MluNI TGGCCA 1 cut(s) 517
MlyI GAGTC 1 cut(s) 275
Mox20I TGGCCA 1 cut(s) 517
Mph1103I ATGCAT 1 cut(s) 965
MscI TGGCCA 1 cut(s) 517
MslI CAYNNNNRTG 4 cut(s) 234, 237, 795, 968
Msp20I TGGCCA 1 cut(s) 517
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 1 cut(s) 362
MunI CAATTG 1 cut(s) 204
Mva1269I GAATGC 1 cut(s) 965
MvaI CCWGG 1 cut(s) 362
MwoI GCNNNNNNNGC 1 cut(s) 251
NcoI CCATGG 1 cut(s) 304
NdeI CATATG 1 cut(s) 412
NdeII GATC 3 cut(s) 83, 552, 803
NlaIII CATG 7 cut(s) 308, 779, 821, 903, 926, 1003, 1111
NlaIV GGNNCC 3 cut(s) 366, 805, 887
NmeAIII GCCGAG 1 cut(s) 1020
NmuCI GTSAC 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 965
NspI RCATGY 1 cut(s) 926
PagI TCATGA 1 cut(s) 999
PctI GAATGC 1 cut(s) 965
PfeI GAWTC 3 cut(s) 259, 279, 623
PflFI GACNNNGTC 1 cut(s) 104
PkrI GCNGC 2 cut(s) 80, 1060
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 360
PspEI GGTNACC 1 cut(s) 396
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 3 cut(s) 366, 805, 887
PspPI GGNCC 2 cut(s) 886, 973
PsuI RGATCY 2 cut(s) 552, 803
PsyI GACNNNGTC 1 cut(s) 104
RseI CAYNNNNRTG 4 cut(s) 234, 237, 795, 968
SatI GCNGC 2 cut(s) 79, 1059
Sau3AI GATC 3 cut(s) 83, 552, 803
Sau96I GGNCC 2 cut(s) 886, 973
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 1 cut(s) 52
SfaNI GCATC 4 cut(s) 232, 738, 799, 853
SinI GGWCC 1 cut(s) 886
SmiMI CAYNNNNRTG 4 cut(s) 234, 237, 795, 968
SsiI CCGC 5 cut(s) 53, 79, 379, 707, 1058
SspMI CTAG 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 3 cut(s) 304, 951, 1012
TaaI ACNGT 2 cut(s) 434, 670
TaqI TCGA 1 cut(s) 420
TauI GCSGC 2 cut(s) 81, 1061
TfiI GAWTC 3 cut(s) 259, 279, 623
TscAI CASTG 2 cut(s) 339, 375
TseFI GTSAC 1 cut(s) 396
Tsp45I GTSAC 1 cut(s) 396
TspDTI ATGAA 1 cut(s) 806
TspGWI ACGGA 3 cut(s) 35, 464, 1037
TspRI CASTG 2 cut(s) 339, 375
Tth111I GACNNNGTC 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 886
XapI RAATTY 5 cut(s) 116, 510, 532, 588, 1032
XceI RCATGY 1 cut(s) 926
XmiI GTMKAC 2 cut(s) 1080, 1095
XspI CTAG 1 cut(s) 246
Zsp2I ATGCAT 1 cut(s) 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.