MD09G1204500.v1.1

non-haem dioxygenase in morphine synthesis N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014284
Physical Location & Seq
Forward (+)
19467793 .. 19470119
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1204500.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGCAACCTCCCCGGAACCAATCTCCGAAAACCCTGTTGATTTTCGTGCCCCGCCGCCTTCTCCCGTTGCCTCTGGTCGCCTATCATCAATCGCAAACGATGAAGTCCTCACCGAATTCCTCGAGCACTCCCTCCAAGTCCCTGACCTCATCTTGCCAGACAAGTTCTTCCCCAAGCAAGTATCTATTGAAAACAATCCTCCCACAATTGATTTTGCAGCCCTGAATTCGGATGGATGTGACGCTAGTCTTTGCAAGGTGGTGGAGTCCATAGCAAGAAATGGATGTTTTCAGCTGGTGAACCATGGAATTTCAAGTGAGCTTGTGGGGTCAGTGCAGGCTGCCGCCACGGGAATCTTTAGCGTGTCACCGGAGAAGAGGGCGGAGGTTACTAGGTCACCGGAGAAGCCATACGGGTTCGAGGAAGTTCATGCTGAGGAGGCTGACAATGTATTGAGTGAAGAGTTTGTTTGGTGTAGAGACCAAGGCTTGAAGTTGGCAATGGATGGAATTGCCCCAATTGGATATTCAAATTTCAGTGAGAAACTAGAGACCCTTATGACAAATATTGAGAAGGTCTGTGAGAAAATTCTACTAAGCTTGTTGATTAATTCTCAAGAAAAAATCATTCATGGAGAAAGCAAAAATGATGAAATGGTGCAAAGGAAAGAAGTTGGGACGGTTTGTTGCTTGTACAAGCATAGCCAGAATGTGTTACAAGATGAATGGGATAGGTCTCTGAGATATGATGTGATTAAGATGCTGATTAGAGGGACTGATTACTCTCATGCCTTGTGTTTCCATATCTGTGATGGTTCTTCAGAGTTCCATGTTTACTCCAAGAAAGGTTGGGTTTCTTTTATCCCAGTTCAAAATGCCCTAATAGTCACCACTGGGGACCAAATACAGGCATGGAGTGGCAGCCAGTATAAGCATGTGATTGGAAGGCCAATCTTTAAAGGTGAGGAGGAATGTATTTCAATGGCTTTCCTCTATTCTCCTCCAAGCTTCATTACAAACTTCCAAAGCAGCAAGGAAAATACTATTTCGCTTGGTCAACAAGTTATAGTGGCTATACTTCTTACTCTTTTCTACCAATTTTCAGTATACTTGTCAAGCCATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

376

Amino Acids

41.76

Weight (kDa)

4.98

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 68 - 150 2.6e-12 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014284)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79760 AT1G79760
fragaria_vesca FvH4_6g32300 FvH4_6g32300
malus_domestica MD09G1204500.v1.1
prunus_persica Prupe.3G060100_v2.0.a1
pyrus_communis pycom09g12140
rosa_chinensis RchiOBHm_Chr2g0140341
rosa_laevigata RLG00000019860
rosa_multiflora Rmu_sc0005446.1_g000032
rosa_roxburghii Rroxscaffold_2G00104920
rosa_rugosa Rorug02G0361700
rosa_samantha Rh2AG413000 Rh2BG423400 Rh2CG399000 Rh2DG432300
rosa_wichuraiana Rw2G033850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1107
AciI CCGC 4 cut(s) 53, 56, 345, 383
AcsI RAATTY 5 cut(s) 116, 226, 309, 532, 588
AcuI CTGAAG 1 cut(s) 804
AfaI GTAC 1 cut(s) 695
AfiI CCNNNNNNNGG 2 cut(s) 229, 907
AgsI TTSAA 6 cut(s) 191, 315, 493, 531, 872, 981
AjuI GAANNNNNNNTTGG 2 cut(s) 511, 543
AluBI AGCT 4 cut(s) 295, 322, 600, 1008
AluI AGCT 4 cut(s) 295, 322, 600, 1008
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 3 cut(s) 474, 545, 741
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 1 cut(s) 947
ApeKI GCWGC 4 cut(s) 218, 341, 921, 1029
ApoI RAATTY 5 cut(s) 116, 226, 309, 532, 588
AseI ATTAAT 1 cut(s) 609
AspS9I GGNCC 1 cut(s) 898
AsuC2I CCSGG 1 cut(s) 14
AsuHPI GGTGA 6 cut(s) 103, 310, 360, 390, 880, 974
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 1 cut(s) 898
BaeGI GKGCMC 1 cut(s) 52
Bbv12I GWGCWC 1 cut(s) 129
BbvCI CCTCAGC 1 cut(s) 435
BbvI GCAGC 4 cut(s) 230, 328, 933, 1041
BccI CCATC 3 cut(s) 227, 500, 806
BcnI CCSGG 1 cut(s) 14
BcoDI GTCTC 3 cut(s) 474, 545, 741
BfaI CTAG 3 cut(s) 246, 393, 548
BisI GCNGC 6 cut(s) 56, 219, 342, 345, 922, 1030
BlsI GCNGC 6 cut(s) 57, 220, 343, 346, 923, 1031
Bme1390I CCNGG 1 cut(s) 14
Bme18I GGWCC 1 cut(s) 898
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 898
BmiI GGNNCC 2 cut(s) 18, 899
BmrFI CCNGG 1 cut(s) 14
BmrI ACTGGG 2 cut(s) 860, 903
BmsI GCATC 1 cut(s) 750
BmuI ACTGGG 2 cut(s) 860, 903
BoxI GACNNNNGTC 1 cut(s) 246
Bpu10I CCTNAGC 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 600
BpuMI CCSGG 1 cut(s) 14
BsaI GGTCTC 3 cut(s) 474, 545, 741
BsaJI CCNNGG 4 cut(s) 12, 304, 348, 484
BsaWI WCCGGW 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 907
Bse1I ACTGG 3 cut(s) 866, 898, 925
Bse3DI GCAATG 1 cut(s) 507
BseDI CCNNGG 4 cut(s) 12, 304, 348, 484
BseGI GGATG 4 cut(s) 238, 242, 290, 511
BseLI CCNNNNNNNGG 2 cut(s) 229, 907
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 2 cut(s) 426, 731
BseNI ACTGG 3 cut(s) 866, 898, 925
BseRI GAGGAG 3 cut(s) 452, 980, 990
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 4 cut(s) 230, 328, 933, 1041
BsgI GTGCAG 1 cut(s) 356
BshFI GGCC 1 cut(s) 949
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 3 cut(s) 14, 371, 401
BslFI GGGAC 4 cut(s) 125, 691, 787, 911
BslI CCNNNNNNNGG 2 cut(s) 229, 907
BsmAI GTCTC 3 cut(s) 474, 545, 741
BsmFI GGGAC 4 cut(s) 125, 691, 787, 911
BsnI GGCC 1 cut(s) 949
Bso31I GGTCTC 3 cut(s) 474, 545, 741
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 2 cut(s) 52, 129
Bsp1407I TGTACA 1 cut(s) 693
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 4 cut(s) 53, 56, 345, 383
BspANI GGCC 1 cut(s) 949
BspCNI CTCAG 2 cut(s) 427, 732
BspLI GGNNCC 2 cut(s) 18, 899
BspTNI GGTCTC 3 cut(s) 474, 545, 741
BsrDI GCAATG 1 cut(s) 507
BsrGI TGTACA 1 cut(s) 693
BsrI ACTGG 3 cut(s) 866, 898, 925
BssECI CCNNGG 4 cut(s) 12, 304, 348, 484
BssNAI GTATAC 1 cut(s) 1108
BssT1I CCWWGG 2 cut(s) 304, 484
Bst1107I GTATAC 1 cut(s) 1108
Bst4CI ACNGT 1 cut(s) 682
Bst6I CTCTTC 2 cut(s) 371, 456
BstAUI TGTACA 1 cut(s) 693
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 3 cut(s) 435, 596, 740
BstDSI CCRYGG 2 cut(s) 304, 348
BstEII GGTNACC 1 cut(s) 396
BstF5I GGATG 4 cut(s) 238, 242, 290, 511
BstMAI GTCTC 3 cut(s) 474, 545, 741
BstMWI GCNNNNNNNGC 1 cut(s) 440
BstNSI RCATGY 1 cut(s) 938
BstPAI GACNNNNGTC 1 cut(s) 246
BstPI GGTNACC 1 cut(s) 396
BstSCI CCNGG 1 cut(s) 12
BstSLI GKGCMC 1 cut(s) 52
BstV1I GCAGC 4 cut(s) 230, 328, 933, 1041
BstZ17I GTATAC 1 cut(s) 1108
BsuRI GGCC 1 cut(s) 949
BtgI CCRYGG 2 cut(s) 304, 348
BtsCI GGATG 4 cut(s) 238, 242, 290, 511
BtsIMutI CAGTG 3 cut(s) 339, 544, 891
Cac8I GCNNGC 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 898
CseI GACGC 1 cut(s) 251
Csp6I GTAC 1 cut(s) 694
CviAII CATG 7 cut(s) 305, 431, 632, 788, 830, 912, 935
CviQI GTAC 1 cut(s) 694
DdeI CTNAG 3 cut(s) 435, 596, 740
DraI TTTAAA 1 cut(s) 958
Eam1104I CTCTTC 2 cut(s) 371, 456
EarI CTCTTC 2 cut(s) 371, 456
EciI GGCGGA 1 cut(s) 398
Eco130I CCWWGG 2 cut(s) 304, 484
Eco31I GGTCTC 3 cut(s) 474, 545, 741
Eco47I GGWCC 1 cut(s) 898
Eco57I CTGAAG 1 cut(s) 804
Eco88I CYCGRG 1 cut(s) 122
Eco91I GGTNACC 1 cut(s) 396
EcoO65I GGTNACC 1 cut(s) 396
EcoRI GAATTC 2 cut(s) 116, 226
EcoT14I CCWWGG 2 cut(s) 304, 484
ErhI CCWWGG 2 cut(s) 304, 484
FaeI CATG 7 cut(s) 308, 434, 635, 791, 833, 915, 938
FaqI GGGAC 4 cut(s) 125, 691, 787, 911
FatI CATG 7 cut(s) 304, 430, 631, 787, 829, 911, 934
FauI CCCGC 1 cut(s) 60
FblI GTMKAC 1 cut(s) 1107
Fnu4HI GCNGC 6 cut(s) 56, 219, 342, 345, 922, 1030
FokI GGATG 4 cut(s) 245, 249, 297, 518
Fsp4HI GCNGC 6 cut(s) 56, 219, 342, 345, 922, 1030
FspBI CTAG 3 cut(s) 246, 393, 548
GluI GCNGC 6 cut(s) 56, 219, 342, 345, 922, 1030
HaeIII GGCC 1 cut(s) 949
HapII CCGG 3 cut(s) 14, 371, 401
HgaI GACGC 1 cut(s) 251
Hin1II CATG 7 cut(s) 308, 434, 635, 791, 833, 915, 938
HincII GTYRAC 1 cut(s) 1058
HindII GTYRAC 1 cut(s) 1058
HindIII AAGCTT 2 cut(s) 598, 1006
HinfI GANTC 2 cut(s) 266, 354
HpaII CCGG 3 cut(s) 14, 371, 401
HphI GGTGA 6 cut(s) 103, 310, 360, 390, 880, 974
Hpy166II GTNNAC 4 cut(s) 301, 835, 1058, 1108
Hpy188I TCNGA 5 cut(s) 28, 232, 741, 823, 1127
Hpy188III TCNNGA 1 cut(s) 617
Hpy8I GTNNAC 4 cut(s) 301, 835, 1058, 1108
HpyAV CCTTC 3 cut(s) 69, 568, 939
HpyCH4III ACNGT 1 cut(s) 682
HpyCH4V TGCA 4 cut(s) 218, 255, 337, 661
HpyF10VI GCNNNNNNNGC 1 cut(s) 440
HpyF3I CTNAG 3 cut(s) 435, 596, 740
Hsp92II CATG 7 cut(s) 308, 434, 635, 791, 833, 915, 938
Lsp1109I GCAGC 4 cut(s) 230, 328, 933, 1041
LweI GCATC 1 cut(s) 750
MaeI CTAG 3 cut(s) 246, 393, 548
MaeIII GTNAC 6 cut(s) 239, 366, 388, 396, 714, 886
MboII GAAGA 4 cut(s) 160, 388, 473, 810
MfeI CAATTG 2 cut(s) 207, 519
MhlI GDGCHC 2 cut(s) 52, 129
MlyI GAGTC 1 cut(s) 275
MseI TTAA 3 cut(s) 609, 756, 957
MslI CAYNNNNRTG 1 cut(s) 807
MspA1I CMGCKG 1 cut(s) 295
MspI CCGG 3 cut(s) 14, 371, 401
MspR9I CCNGG 1 cut(s) 14
MunI CAATTG 2 cut(s) 207, 519
MwoI GCNNNNNNNGC 1 cut(s) 440
NciI CCSGG 1 cut(s) 14
NcoI CCATGG 1 cut(s) 304
NlaIII CATG 7 cut(s) 308, 434, 635, 791, 833, 915, 938
NlaIV GGNNCC 2 cut(s) 18, 899
NmuCI GTSAC 4 cut(s) 239, 366, 396, 886
NspI RCATGY 1 cut(s) 938
PaeR7I CTCGAG 1 cut(s) 122
PfeI GAWTC 1 cut(s) 354
PkrI GCNGC 6 cut(s) 57, 220, 343, 346, 923, 1031
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PshAI GACNNNNGTC 1 cut(s) 246
PshBI ATTAAT 1 cut(s) 609
PspEI GGTNACC 1 cut(s) 396
PspN4I GGNNCC 2 cut(s) 18, 899
PspPI GGNCC 1 cut(s) 898
PspXI VCTCGAGB 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 295
RsaI GTAC 1 cut(s) 695
RsaNI GTAC 1 cut(s) 694
RseI CAYNNNNRTG 1 cut(s) 807
SaqAI TTAA 3 cut(s) 609, 756, 957
SatI GCNGC 6 cut(s) 56, 219, 342, 345, 922, 1030
Sau96I GGNCC 1 cut(s) 898
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 1 cut(s) 14
SduI GDGCHC 2 cut(s) 52, 129
SfaNI GCATC 1 cut(s) 750
Sfr274I CTCGAG 1 cut(s) 122
SinI GGWCC 1 cut(s) 898
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 807
SmlI CTYRAG 2 cut(s) 122, 615
SmoI CTYRAG 2 cut(s) 122, 615
SsiI CCGC 4 cut(s) 53, 56, 345, 383
SspI AATATT 1 cut(s) 568
SspMI CTAG 3 cut(s) 246, 393, 548
StyD4I CCNGG 1 cut(s) 12
StyI CCWWGG 2 cut(s) 304, 484
TaaI ACNGT 1 cut(s) 682
TaqI TCGA 2 cut(s) 123, 420
TatI WGTACW 1 cut(s) 693
TauI GCSGC 2 cut(s) 58, 347
TfiI GAWTC 1 cut(s) 354
Tru1I TTAA 3 cut(s) 609, 756, 957
Tru9I TTAA 3 cut(s) 609, 756, 957
TscAI CASTG 3 cut(s) 339, 544, 898
TseFI GTSAC 4 cut(s) 239, 366, 396, 886
TseI GCWGC 4 cut(s) 218, 341, 921, 1029
Tsp45I GTSAC 4 cut(s) 239, 366, 396, 886
TspDTI ATGAA 6 cut(s) 117, 419, 620, 666, 738, 1000
TspRI CASTG 3 cut(s) 339, 544, 898
VpaK11BI GGWCC 1 cut(s) 898
VspI ATTAAT 1 cut(s) 609
XapI RAATTY 5 cut(s) 116, 226, 309, 532, 588
XceI RCATGY 1 cut(s) 938
XcmI CCANNNNNNNNNTGG 1 cut(s) 809
XhoI CTCGAG 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 1107
XspI CTAG 3 cut(s) 246, 393, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.