Prupe.3G060100_v2.0.a1

non-haem dioxygenase in morphine synthesis N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0014284
Physical Location & Seq
Forward (+)
4326582 .. 4328728
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G060100.1

Sequence Viewer

Length: 1116 bp
ATGGCAGCCTCACCTCAACCCGTCTCTGAAAACCCAATTGATTTTCGTGCCCCACCGCCTTCTCCCATCGCCTCAGGCCGCCGATCATTAGTCACAAACGATGAAGTCCTCACCGAATACCTCGAGCACTCCCTCCAAGTCCCTGACCTCATTTTGCCAGACAAGTTCTTCCCTAAACAAAATTCACTTGAAAATCCCCCCACCATTGATTTGTTAGCATTGAATCTGGATGAGTTTGATGCTAGTCTTTGCAAGGTTGTGGAGTCCATAGCAAGAAACGGGTGTTTTCAGCTGGTGAACCATGGAATTTCAAGTGAGCTTGTGAGCTCTGTGCAGGCTGCAGCCACCGGAATCTTCCAGGTACCGCCGGAGAAGAGGGCGGAGATAACCCGGTTACCGGAGAAGCCATACGGGTTCGAGGAGGTTCATGGTGAGGAGGTTGGCAGTGAACTGAGTGAAGAGTTTGTTTGGTGTAGAGACCAAGGTTTGAAGTTGACAATGGAGAGAATTGCACCAATTGGATATTCAAATTTCGGGGAGAAACTGGAGACCCTAATGACAGATATTGAGAAGGTTTCTGGGAAAATCCTACCAAGCTTATTGAAAAATTCTCAAACAAAATTTGTTTATGGAAATGATGATATGGTGCAAGGGAAAGAACTTGGGACAGTCTGTTGCCTGTACAGGCACAGCCAAAATGTTTTAGAAGATGAATGGGACAGCTCTTTAAGATATGATGTGATTAAGATGTTGATTAGAGGAACTGACTATTCTCATGCCTTGTGTTTGCATCTTTGTGATGGTTCTTCAGAGTTCCATGTTTACTCCAAGAAAGGTTGGGTTTCTTTTACCCCAGATAAAAATGCTCTAGTAGTCACCACTGGGGATCAAATACAGGCATGGAGTGGTGGCCAGTATAAGCATGTGATAGGAAGGCCAATCTTTAAAGGTGAGGAGGACTGCATCTCAATGGCTTTTCTCTATTCTCCTCCAACCTTTATTTCAAACTCCCAAAGCAACAAGGAAAAGACTATATCACTTGGTGAACAAGCTATAATGGCCATATTTTTAACTCTTGTCTACCAATTTTTAGTGTACTTCTACAGAAATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

372

Amino Acids

41.6

Weight (kDa)

4.99

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014284)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79760 AT1G79760
fragaria_vesca FvH4_6g32300 FvH4_6g32300
malus_domestica MD09G1204500.v1.1
prunus_persica Prupe.3G060100_v2.0.a1
pyrus_communis pycom09g12140
rosa_chinensis RchiOBHm_Chr2g0140341
rosa_laevigata RLG00000019860
rosa_multiflora Rmu_sc0005446.1_g000032
rosa_roxburghii Rroxscaffold_2G00104920
rosa_rugosa Rorug02G0361700
rosa_samantha Rh2AG413000 Rh2BG423400 Rh2CG399000 Rh2DG432300
rosa_wichuraiana Rw2G033850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 361
AccB1I GGYRCC 1 cut(s) 361
AccI GTMKAC 1 cut(s) 1080
AciI CCGC 4 cut(s) 56, 79, 365, 380
AclWI GGATC 1 cut(s) 894
AcoI YGGCCR 2 cut(s) 910, 1059
AcsI RAATTY 6 cut(s) 181, 306, 529, 607, 620, 1108
AcuI CTGAAG 1 cut(s) 792
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 3 cut(s) 363, 683, 1097
AfiI CCNNNNNNNGG 1 cut(s) 397
AgsI TTSAA 7 cut(s) 191, 223, 312, 490, 528, 604, 1005
AjnI CCWGG 1 cut(s) 357
AjuI GAANNNNNNNTTGG 4 cut(s) 508, 540, 985, 1017
AluBI AGCT 6 cut(s) 292, 319, 327, 597, 723, 1052
AluI AGCT 6 cut(s) 292, 319, 327, 597, 723, 1052
Alw21I GWGCWC 2 cut(s) 129, 329
Alw26I GTCTC 3 cut(s) 28, 471, 542
AlwI GGATC 1 cut(s) 894
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 4 cut(s) 76, 910, 935, 1059
ApeKI GCWGC 3 cut(s) 5, 338, 341
ApoI RAATTY 6 cut(s) 181, 306, 529, 607, 620, 1108
Asp718I GGTACC 1 cut(s) 361
AsuC2I CCSGG 1 cut(s) 391
AsuHPI GGTGA 7 cut(s) 3, 103, 307, 443, 868, 962, 1055
AvaI CYCGRG 1 cut(s) 122
AxyI CCTNAGG 1 cut(s) 73
BaeGI GKGCMC 1 cut(s) 52
BalI TGGCCA 2 cut(s) 912, 1061
BanI GGYRCC 1 cut(s) 361
BanII GRGCYC 1 cut(s) 329
Bbv12I GWGCWC 2 cut(s) 129, 329
BbvI GCAGC 3 cut(s) 17, 325, 353
BccI CCATC 2 cut(s) 74, 794
BciT130I CCWGG 1 cut(s) 359
BcnI CCSGG 1 cut(s) 391
BcoDI GTCTC 3 cut(s) 28, 471, 542
BfaI CTAG 2 cut(s) 243, 869
BfmI CTRYAG 2 cut(s) 339, 1102
BisI GCNGC 4 cut(s) 6, 79, 339, 342
BlsI GCNGC 4 cut(s) 7, 80, 340, 343
Bme1390I CCNGG 2 cut(s) 359, 391
BmeT110I CYCGRG 1 cut(s) 122
BmiI GGNNCC 1 cut(s) 363
BmrFI CCNGG 2 cut(s) 359, 391
BmrI ACTGGG 1 cut(s) 891
BmsI GCATC 3 cut(s) 229, 799, 972
BmuI ACTGGG 1 cut(s) 891
BpmI CTGGAG 1 cut(s) 566
BpuMI CCSGG 1 cut(s) 391
BsaI GGTCTC 2 cut(s) 471, 542
BsaJI CCNNGG 2 cut(s) 301, 481
BsaWI WCCGGW 2 cut(s) 347, 397
Bsc4I CCNNNNNNNGG 1 cut(s) 397
Bse1I ACTGG 3 cut(s) 549, 886, 913
Bse21I CCTNAGG 1 cut(s) 73
BseBI CCWGG 1 cut(s) 359
BseDI CCNNGG 2 cut(s) 301, 481
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 1 cut(s) 397
BseMII CTCAG 2 cut(s) 87, 443
BseNI ACTGG 3 cut(s) 549, 886, 913
BseRI GAGGAG 4 cut(s) 434, 449, 968, 978
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 3 cut(s) 17, 325, 353
BsgI GTGCAG 1 cut(s) 353
BshFI GGCC 4 cut(s) 78, 912, 937, 1061
BshNI GGYRCC 1 cut(s) 361
BsiHKAI GWGCWC 2 cut(s) 129, 329
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 4 cut(s) 348, 368, 391, 398
BslFI GGGAC 3 cut(s) 125, 679, 731
BslI CCNNNNNNNGG 1 cut(s) 397
BsmAI GTCTC 3 cut(s) 28, 471, 542
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 3 cut(s) 125, 679, 731
BsnI GGCC 4 cut(s) 78, 912, 937, 1061
Bso31I GGTCTC 2 cut(s) 471, 542
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 3 cut(s) 52, 129, 329
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 2 cut(s) 83, 886
Bsp19I CCATGG 1 cut(s) 301
BspACI CCGC 4 cut(s) 56, 79, 365, 380
BspANI GGCC 4 cut(s) 78, 912, 937, 1061
BspCNI CTCAG 2 cut(s) 86, 444
BspLI GGNNCC 1 cut(s) 363
BspMAI CTGCAG 1 cut(s) 343
BspPI GGATC 1 cut(s) 894
BspT107I GGYRCC 1 cut(s) 361
BspTNI GGTCTC 2 cut(s) 471, 542
BsrGI TGTACA 1 cut(s) 681
BsrI ACTGG 3 cut(s) 549, 886, 913
BssECI CCNNGG 2 cut(s) 301, 481
BssMI GATC 2 cut(s) 83, 886
BssT1I CCWWGG 2 cut(s) 301, 481
Bst2UI CCWGG 1 cut(s) 359
Bst4CI ACNGT 1 cut(s) 670
Bst6I CTCTTC 2 cut(s) 368, 453
BstAUI TGTACA 1 cut(s) 681
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 2 cut(s) 73, 452
BstDSI CCRYGG 1 cut(s) 301
BstEII GGTNACC 1 cut(s) 393
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 2 cut(s) 86, 889
BstMAI GTCTC 3 cut(s) 28, 471, 542
BstMBI GATC 2 cut(s) 83, 886
BstMWI GCNNNNNNNGC 1 cut(s) 1058
BstNI CCWGG 1 cut(s) 359
BstNSI RCATGY 1 cut(s) 926
BstPI GGTNACC 1 cut(s) 393
BstSCI CCNGG 2 cut(s) 357, 389
BstSFI CTRYAG 2 cut(s) 339, 1102
BstSLI GKGCMC 1 cut(s) 52
BstV1I GCAGC 3 cut(s) 17, 325, 353
Bsu36I CCTNAGG 1 cut(s) 73
BsuRI GGCC 4 cut(s) 78, 912, 937, 1061
BtgI CCRYGG 1 cut(s) 301
BtgZI GCGATG 1 cut(s) 52
BtsCI GGATG 1 cut(s) 235
BtsI GCAGTG 1 cut(s) 451
BtsIMutI CAGTG 2 cut(s) 451, 879
Cac8I GCNNGC 1 cut(s) 336
Csp6I GTAC 3 cut(s) 362, 682, 1096
CviAII CATG 6 cut(s) 302, 428, 776, 818, 900, 923
CviQI GTAC 3 cut(s) 362, 682, 1096
DdeI CTNAG 2 cut(s) 73, 452
DpnI GATC 2 cut(s) 85, 888
DpnII GATC 2 cut(s) 83, 886
DraI TTTAAA 1 cut(s) 946
DraIII CACNNNGTG 1 cut(s) 1043
EaeI YGGCCR 2 cut(s) 910, 1059
Eam1104I CTCTTC 2 cut(s) 368, 453
EarI CTCTTC 2 cut(s) 368, 453
EciI GGCGGA 1 cut(s) 395
Ecl136II GAGCTC 1 cut(s) 327
Eco130I CCWWGG 2 cut(s) 301, 481
Eco24I GRGCYC 1 cut(s) 329
Eco31I GGTCTC 2 cut(s) 471, 542
Eco53kI GAGCTC 1 cut(s) 327
Eco57I CTGAAG 1 cut(s) 792
Eco81I CCTNAGG 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 122
Eco91I GGTNACC 1 cut(s) 393
EcoICRI GAGCTC 1 cut(s) 327
EcoO65I GGTNACC 1 cut(s) 393
EcoRII CCWGG 1 cut(s) 357
EcoT14I CCWWGG 2 cut(s) 301, 481
EcoT38I GRGCYC 1 cut(s) 329
ErhI CCWWGG 2 cut(s) 301, 481
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 6 cut(s) 305, 431, 779, 821, 903, 926
FaqI GGGAC 3 cut(s) 125, 679, 731
FatI CATG 6 cut(s) 301, 427, 775, 817, 899, 922
FblI GTMKAC 1 cut(s) 1080
Fnu4HI GCNGC 4 cut(s) 6, 79, 339, 342
FokI GGATG 1 cut(s) 242
FriOI GRGCYC 1 cut(s) 329
Fsp4HI GCNGC 4 cut(s) 6, 79, 339, 342
FspBI CTAG 2 cut(s) 243, 869
GluI GCNGC 4 cut(s) 6, 79, 339, 342
GsuI CTGGAG 1 cut(s) 566
HaeIII GGCC 4 cut(s) 78, 912, 937, 1061
HapII CCGG 4 cut(s) 348, 368, 391, 398
Hin1II CATG 6 cut(s) 305, 431, 779, 821, 903, 926
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HindIII AAGCTT 1 cut(s) 595
HinfI GANTC 3 cut(s) 223, 263, 351
HpaII CCGG 4 cut(s) 348, 368, 391, 398
HphI GGTGA 7 cut(s) 3, 103, 307, 443, 868, 962, 1055
Hpy166II GTNNAC 7 cut(s) 298, 449, 495, 823, 1046, 1081, 1096
Hpy188I TCNGA 3 cut(s) 28, 811, 1115
Hpy188III TCNNGA 1 cut(s) 227
Hpy8I GTNNAC 7 cut(s) 298, 449, 495, 823, 1046, 1081, 1096
HpyAV CCTTC 3 cut(s) 69, 565, 927
HpyCH4III ACNGT 1 cut(s) 670
HpyCH4V TGCA 7 cut(s) 252, 334, 341, 512, 649, 790, 963
HpyF10VI GCNNNNNNNGC 1 cut(s) 1058
HpyF3I CTNAG 2 cut(s) 73, 452
Hsp92II CATG 6 cut(s) 305, 431, 779, 821, 903, 926
KpnI GGTACC 1 cut(s) 365
Kzo9I GATC 2 cut(s) 83, 886
Lsp1109I GCAGC 3 cut(s) 17, 325, 353
LweI GCATC 3 cut(s) 229, 799, 972
MaeI CTAG 2 cut(s) 243, 869
MaeIII GTNAC 3 cut(s) 91, 393, 874
MalI GATC 2 cut(s) 85, 888
MboI GATC 2 cut(s) 83, 886
MboII GAAGA 6 cut(s) 160, 346, 385, 470, 719, 798
MfeI CAATTG 2 cut(s) 36, 516
MhlI GDGCHC 3 cut(s) 52, 129, 329
MlsI TGGCCA 2 cut(s) 912, 1061
MluNI TGGCCA 2 cut(s) 912, 1061
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 1016
Mox20I TGGCCA 2 cut(s) 912, 1061
MscI TGGCCA 2 cut(s) 912, 1061
MseI TTAA 4 cut(s) 728, 744, 945, 1070
MslI CAYNNNNRTG 2 cut(s) 795, 968
Msp20I TGGCCA 2 cut(s) 912, 1061
MspA1I CMGCKG 1 cut(s) 292
MspI CCGG 4 cut(s) 348, 368, 391, 398
MspR9I CCNGG 2 cut(s) 359, 391
MunI CAATTG 2 cut(s) 36, 516
MvaI CCWGG 1 cut(s) 359
MwoI GCNNNNNNNGC 1 cut(s) 1058
NciI CCSGG 1 cut(s) 391
NcoI CCATGG 1 cut(s) 301
NdeII GATC 2 cut(s) 83, 886
NlaIII CATG 6 cut(s) 305, 431, 779, 821, 903, 926
NlaIV GGNNCC 1 cut(s) 363
NmuCI GTSAC 2 cut(s) 91, 874
NspI RCATGY 1 cut(s) 926
PaeR7I CTCGAG 1 cut(s) 122
PfeI GAWTC 2 cut(s) 223, 351
PkrI GCNGC 4 cut(s) 7, 80, 340, 343
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
Psp124BI GAGCTC 1 cut(s) 329
Psp6I CCWGG 1 cut(s) 357
PspEI GGTNACC 1 cut(s) 393
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 1 cut(s) 363
PspXI VCTCGAGB 1 cut(s) 122
PstI CTGCAG 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 292
RsaI GTAC 3 cut(s) 363, 683, 1097
RsaNI GTAC 3 cut(s) 362, 682, 1096
RseI CAYNNNNRTG 2 cut(s) 795, 968
SacI GAGCTC 1 cut(s) 329
SaqAI TTAA 4 cut(s) 728, 744, 945, 1070
SatI GCNGC 4 cut(s) 6, 79, 339, 342
Sau3AI GATC 2 cut(s) 83, 886
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 2 cut(s) 359, 391
SduI GDGCHC 3 cut(s) 52, 129, 329
SfaNI GCATC 3 cut(s) 229, 799, 972
SfcI CTRYAG 2 cut(s) 339, 1102
Sfr274I CTCGAG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 2 cut(s) 795, 968
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SsiI CCGC 4 cut(s) 56, 79, 365, 380
SspMI CTAG 2 cut(s) 243, 869
SstI GAGCTC 1 cut(s) 329
StyD4I CCNGG 2 cut(s) 357, 389
StyI CCWWGG 2 cut(s) 301, 481
TaaI ACNGT 1 cut(s) 670
TaqI TCGA 2 cut(s) 123, 417
TatI WGTACW 2 cut(s) 681, 1095
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 2 cut(s) 223, 351
Tru1I TTAA 4 cut(s) 728, 744, 945, 1070
Tru9I TTAA 4 cut(s) 728, 744, 945, 1070
TscAI CASTG 2 cut(s) 451, 886
TseFI GTSAC 2 cut(s) 91, 874
TseI GCWGC 3 cut(s) 5, 338, 341
Tsp45I GTSAC 2 cut(s) 91, 874
TspDTI ATGAA 3 cut(s) 117, 416, 726
TspRI CASTG 2 cut(s) 451, 886
XapI RAATTY 6 cut(s) 181, 306, 529, 607, 620, 1108
XceI RCATGY 1 cut(s) 926
XhoI CTCGAG 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 1080
XspI CTAG 2 cut(s) 243, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.