Rroxscaffold_2G00104920

non-haem dioxygenase in morphine synthesis N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014284
Physical Location & Seq
Forward (+)
28297043 .. 28299375
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00104920.1

Sequence Viewer

Length: 1131 bp
ATGGCAAGCTTACCTGAACCCGTCCACGAAAACCCCATTGATTTTCGTGCCCCGCCACCTTCTCCAGTTGCCTCTGGCCGGCGATCATCCCTCACAAACGACGATGTCATTACCGAATTCCTCCAGCATTCCCTCAATGTCCCTAACCTCATCTTGCCAGACAAATTCTTTCCAAAACAAAAATCAGTTGAAAATCCCCCCACAATTGATTTTGAAACATTGTGTTCAGATCAATATGATGATGCTAGTGTTACCAAGATTCTTGAGTCAATAGCAAGAATTGGGTGTTTTCAGTTAGTGAACCATGGAATTTCAATTGATTTTGTAAGGTCAGTGCAGGCTGAAGCCACCGGGATTTTCCAGGTGCCACTGGAGAAGAGGGCGGACATAACAAGGTCACCGGAGAAGCCGTATGGGTTCGAGGAAGTTCATGGTGAGGAAGCTGACACCGAATTGAGTGAAGAGTTCGTTTGGTGTAGAGAACAAGGTTTGAAGTTGGCAATGGAGGGAATTTGGCCAATTGGGTATTCAAATTATTCAAATTTCAGCAAGAAATTGGAGACCCTTATGACAGACATTGAGAAGATTTGTGAGAGAATTCTACAAAGCTTATTGAACTTTAAACAAAAACAATCTGTTAATGGAAATGCTTTGGTTCAAGGAAAAGAACTTGGGACAGTTTGTTGTTTTTACAAGCACAATCAGAATGTTTTAGCTGATAAGTGGGATAGCTATCTGAGATATGATGTGATTAGGATGCTGATAAGAGGAACTGATTACTCTCATGCCTTGTGTCTGCATCTTTGTGATGGATCCTCAGAGTTTCATGTTTACTCCAAGAAAGGTTGGGTTTCTTCTTTTAGCCCAGATAAAGATGCTCTTGTAGTTACCACAGGGGACCAAATGCAGGCATGGAGTGGAAGCCAATACAAGCATGTGATAGGAAGGCCAATCTTTAAGGCTGAGAAGGAGGAATGCATTTCAATGGCCCTTCTCTATTCCCCTCCAAGCACCATGACAAACTCCCAAAGCAACAAGGAAAAGAAAATCTTACTCGGTCAACAATTTATATTGGCTATATTACTAACTCTTGTCTACCAATTTTCAGTATACTTCTACAGAAATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

376

Amino Acids

42.7

Weight (kDa)

5.56

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 67 - 152 2.6e-13 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014284)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79760 AT1G79760
fragaria_vesca FvH4_6g32300 FvH4_6g32300
malus_domestica MD09G1204500.v1.1
prunus_persica Prupe.3G060100_v2.0.a1
pyrus_communis pycom09g12140
rosa_chinensis RchiOBHm_Chr2g0140341
rosa_laevigata RLG00000019860
rosa_multiflora Rmu_sc0005446.1_g000032
rosa_roxburghii Rroxscaffold_2G00104920
rosa_rugosa Rorug02G0361700
rosa_samantha Rh2AG413000 Rh2BG423400 Rh2CG399000 Rh2DG432300
rosa_wichuraiana Rw2G033850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 364
AccI GTMKAC 2 cut(s) 1095, 1110
AciI CCGC 2 cut(s) 53, 383
AclWI GGATC 2 cut(s) 807, 820
AcoI YGGCCR 2 cut(s) 76, 515
AcsI RAATTY 7 cut(s) 116, 164, 309, 510, 541, 597, 1123
AcuI CTGAAG 1 cut(s) 363
AfiI CCNNNNNNNGG 2 cut(s) 78, 907
AgsI TTSAA 9 cut(s) 191, 215, 315, 493, 531, 540, 616, 659, 984
AjnI CCWGG 1 cut(s) 360
AjuI GAANNNNNNNTTGG 2 cut(s) 511, 543
AluBI AGCT 5 cut(s) 9, 443, 609, 716, 732
AluI AGCT 5 cut(s) 9, 443, 609, 716, 732
Alw26I GTCTC 1 cut(s) 554
AlwI GGATC 2 cut(s) 807, 820
AoxI GGCC 4 cut(s) 76, 515, 947, 987
ApoI RAATTY 7 cut(s) 116, 164, 309, 510, 541, 597, 1123
AspS9I GGNCC 2 cut(s) 898, 988
AsuC2I CCSGG 1 cut(s) 352
AsuHPI GGTGA 2 cut(s) 390, 446
AvaII GGWCC 1 cut(s) 898
BaeGI GKGCMC 1 cut(s) 52
BalI TGGCCA 1 cut(s) 517
BamHI GGATCC 1 cut(s) 812
BanI GGYRCC 1 cut(s) 364
BccI CCATC 1 cut(s) 803
BceAI ACGGC 1 cut(s) 394
BciT130I CCWGG 1 cut(s) 362
BcnI CCSGG 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 554
BfaI CTAG 1 cut(s) 246
BfmI CTRYAG 1 cut(s) 1117
Bme1390I CCNGG 2 cut(s) 352, 362
Bme18I GGWCC 1 cut(s) 898
BmgT120I GGNCC 2 cut(s) 898, 988
BmiI GGNNCC 3 cut(s) 366, 814, 899
BmrFI CCNGG 2 cut(s) 352, 362
BmsI GCATC 4 cut(s) 232, 747, 808, 865
BpmI CTGGAG 3 cut(s) 48, 107, 392
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 352
BsaBI GATNNNNATC 1 cut(s) 732
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 1 cut(s) 304
BsaWI WCCGGW 1 cut(s) 400
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 907
Bse118I RCCGGY 1 cut(s) 78
Bse1I ACTGG 2 cut(s) 65, 375
Bse3DI GCAATG 1 cut(s) 507
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 1 cut(s) 304
BseGI GGATG 2 cut(s) 86, 762
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 2 cut(s) 78, 907
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 3 cut(s) 728, 831, 954
BseNI ACTGG 2 cut(s) 65, 375
BseSI GKGCMC 1 cut(s) 52
BsgI GTGCAG 1 cut(s) 356
BshFI GGCC 4 cut(s) 78, 517, 949, 989
BshNI GGYRCC 1 cut(s) 364
BsiSI CCGG 3 cut(s) 79, 351, 401
BslFI GGGAC 3 cut(s) 125, 688, 911
BslI CCNNNNNNNGG 2 cut(s) 78, 907
BsmAI GTCTC 1 cut(s) 554
BsmFI GGGAC 3 cut(s) 125, 688, 911
BsmI GAATGC 2 cut(s) 127, 980
BsnI GGCC 4 cut(s) 78, 517, 949, 989
Bso31I GGTCTC 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 52
Bsp143I GATC 3 cut(s) 83, 229, 812
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 2 cut(s) 53, 383
BspANI GGCC 4 cut(s) 78, 517, 949, 989
BspCNI CTCAG 3 cut(s) 729, 830, 955
BspLI GGNNCC 3 cut(s) 366, 814, 899
BspPI GGATC 2 cut(s) 807, 820
BspT107I GGYRCC 1 cut(s) 364
BspTNI GGTCTC 1 cut(s) 554
BsrDI GCAATG 1 cut(s) 507
BsrFI RCCGGY 1 cut(s) 78
BsrI ACTGG 2 cut(s) 65, 375
BssAI RCCGGY 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 304
BssMI GATC 3 cut(s) 83, 229, 812
BssNAI GTATAC 1 cut(s) 1111
BssT1I CCWWGG 1 cut(s) 304
Bst1107I GTATAC 1 cut(s) 1111
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 1 cut(s) 679
Bst6I CTCTTC 2 cut(s) 371, 456
BstC8I GCNNGC 4 cut(s) 7, 80, 339, 909
BstDEI CTNAG 3 cut(s) 737, 817, 963
BstDSI CCRYGG 1 cut(s) 304
BstEII GGTNACC 1 cut(s) 396
BstF5I GGATG 2 cut(s) 86, 762
BstKTI GATC 3 cut(s) 86, 232, 815
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 3 cut(s) 83, 229, 812
BstNI CCWGG 1 cut(s) 362
BstNSI RCATGY 1 cut(s) 938
BstPI GGTNACC 1 cut(s) 396
BstSCI CCNGG 2 cut(s) 350, 360
BstSFI CTRYAG 1 cut(s) 1117
BstSLI GKGCMC 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 812
BstYI RGATCY 1 cut(s) 812
BstZ17I GTATAC 1 cut(s) 1111
BsuRI GGCC 4 cut(s) 78, 517, 949, 989
BtgI CCRYGG 1 cut(s) 304
BtsCI GGATG 2 cut(s) 86, 762
BtsIMutI CAGTG 2 cut(s) 339, 368
Cac8I GCNNGC 4 cut(s) 7, 80, 339, 909
Cfr10I RCCGGY 1 cut(s) 78
Cfr13I GGNCC 2 cut(s) 898, 988
CviAII CATG 7 cut(s) 305, 431, 785, 827, 912, 935, 1015
DdeI CTNAG 3 cut(s) 737, 817, 963
DpnI GATC 3 cut(s) 85, 231, 814
DpnII GATC 3 cut(s) 83, 229, 812
DraI TTTAAA 1 cut(s) 622
EaeI YGGCCR 2 cut(s) 76, 515
Eam1104I CTCTTC 2 cut(s) 371, 456
EarI CTCTTC 2 cut(s) 371, 456
EciI GGCGGA 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 304
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 898
Eco57I CTGAAG 1 cut(s) 363
Eco91I GGTNACC 1 cut(s) 396
EcoO65I GGTNACC 1 cut(s) 396
EcoRI GAATTC 2 cut(s) 116, 597
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 304
EcoT22I ATGCAT 1 cut(s) 980
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 7 cut(s) 308, 434, 788, 830, 915, 938, 1018
FalI AAGNNNNNCTT 4 cut(s) 864, 896, 1034, 1066
FaqI GGGAC 3 cut(s) 125, 688, 911
FatI CATG 7 cut(s) 304, 430, 784, 826, 911, 934, 1014
FauI CCCGC 1 cut(s) 60
FblI GTMKAC 2 cut(s) 1095, 1110
FokI GGATG 2 cut(s) 73, 769
FspBI CTAG 1 cut(s) 246
GsuI CTGGAG 3 cut(s) 48, 107, 392
HaeIII GGCC 4 cut(s) 78, 517, 949, 989
HapII CCGG 3 cut(s) 79, 351, 401
Hin1II CATG 7 cut(s) 308, 434, 788, 830, 915, 938, 1018
HincII GTYRAC 1 cut(s) 1061
HindII GTYRAC 1 cut(s) 1061
HindIII AAGCTT 2 cut(s) 7, 607
HinfI GANTC 2 cut(s) 259, 266
HpaII CCGG 3 cut(s) 79, 351, 401
HphI GGTGA 2 cut(s) 390, 446
Hpy166II GTNNAC 6 cut(s) 25, 301, 832, 1061, 1096, 1111
Hpy188I TCNGA 4 cut(s) 229, 705, 738, 820
Hpy188III TCNNGA 1 cut(s) 263
Hpy8I GTNNAC 6 cut(s) 25, 301, 832, 1061, 1096, 1111
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 4 cut(s) 69, 939, 961, 1001
HpyCH4III ACNGT 1 cut(s) 679
HpyCH4V TGCA 4 cut(s) 337, 799, 907, 978
HpyF3I CTNAG 3 cut(s) 737, 817, 963
Hsp92II CATG 7 cut(s) 308, 434, 788, 830, 915, 938, 1018
KroI GCCGGC 1 cut(s) 78
KroNI GCCGGC 1 cut(s) 80
Kzo9I GATC 3 cut(s) 83, 229, 812
LweI GCATC 4 cut(s) 232, 747, 808, 865
MaeI CTAG 1 cut(s) 246
MaeIII GTNAC 3 cut(s) 250, 396, 886
MalI GATC 3 cut(s) 85, 231, 814
MboI GATC 3 cut(s) 83, 229, 812
MboII GAAGA 4 cut(s) 388, 473, 595, 846
MfeI CAATTG 3 cut(s) 204, 315, 519
MflI RGATCY 1 cut(s) 812
MhlI GDGCHC 1 cut(s) 52
MlsI TGGCCA 1 cut(s) 517
MluNI TGGCCA 1 cut(s) 517
MlyI GAGTC 1 cut(s) 275
Mox20I TGGCCA 1 cut(s) 517
Mph1103I ATGCAT 1 cut(s) 980
MroNI GCCGGC 1 cut(s) 78
MscI TGGCCA 1 cut(s) 517
MseI TTAA 3 cut(s) 621, 639, 957
MslI CAYNNNNRTG 2 cut(s) 804, 983
Msp20I TGGCCA 1 cut(s) 517
MspI CCGG 3 cut(s) 79, 351, 401
MspR9I CCNGG 2 cut(s) 352, 362
MunI CAATTG 3 cut(s) 204, 315, 519
Mva1269I GAATGC 2 cut(s) 127, 980
MvaI CCWGG 1 cut(s) 362
NaeI GCCGGC 1 cut(s) 80
NciI CCSGG 1 cut(s) 352
NcoI CCATGG 1 cut(s) 304
NdeII GATC 3 cut(s) 83, 229, 812
NgoMIV GCCGGC 1 cut(s) 78
NlaIII CATG 7 cut(s) 308, 434, 788, 830, 915, 938, 1018
NlaIV GGNNCC 3 cut(s) 366, 814, 899
NmuCI GTSAC 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 980
NspI RCATGY 1 cut(s) 938
PctI GAATGC 2 cut(s) 127, 980
PdiI GCCGGC 1 cut(s) 80
PfeI GAWTC 1 cut(s) 259
PflFI GACNNNGTC 1 cut(s) 104
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 360
PspEI GGTNACC 1 cut(s) 396
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 3 cut(s) 366, 814, 899
PspPI GGNCC 2 cut(s) 898, 988
PsuI RGATCY 1 cut(s) 812
PsyI GACNNNGTC 1 cut(s) 104
RseI CAYNNNNRTG 2 cut(s) 804, 983
SaqAI TTAA 3 cut(s) 621, 639, 957
Sau3AI GATC 3 cut(s) 83, 229, 812
Sau96I GGNCC 2 cut(s) 898, 988
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 2 cut(s) 352, 362
SduI GDGCHC 1 cut(s) 52
SfaNI GCATC 4 cut(s) 232, 747, 808, 865
SfcI CTRYAG 1 cut(s) 1117
SinI GGWCC 1 cut(s) 898
SmiMI CAYNNNNRTG 2 cut(s) 804, 983
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SsiI CCGC 2 cut(s) 53, 383
SspMI CTAG 1 cut(s) 246
StyD4I CCNGG 2 cut(s) 350, 360
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 1 cut(s) 679
TaqI TCGA 1 cut(s) 420
TaqII GACCGA 1 cut(s) 1046
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 3 cut(s) 621, 639, 957
Tru9I TTAA 3 cut(s) 621, 639, 957
TscAI CASTG 2 cut(s) 339, 375
TseFI GTSAC 1 cut(s) 396
Tsp45I GTSAC 1 cut(s) 396
TspDTI ATGAA 2 cut(s) 419, 815
TspRI CASTG 2 cut(s) 339, 375
Tth111I GACNNNGTC 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 898
XapI RAATTY 7 cut(s) 116, 164, 309, 510, 541, 597, 1123
XceI RCATGY 1 cut(s) 938
XmiI GTMKAC 2 cut(s) 1095, 1110
XspI CTAG 1 cut(s) 246
Zsp2I ATGCAT 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.